LetaA.01391.a

Protein kinase, putative

CENTER ID: LetaA.01391.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.01391.a.HZ3.GE43733 kinase domain #2 (Jensen) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP36.0740

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTEIESHIL KKYEIQTQLG QGAYGIVWRA LERKHNRVVA LKKIYDAFQN STDAQRTFRE IMFLHRLHHP NIIKLLHVHR AFNDRDIYLV FEYMETDLHV VIRANILEDI HKQFIIYQLL KTMKYLHSAE ILHRDMKPSN LLVNSDCTMK VADFGLARSI LSLEGEQVSR PVLTDYIATR WYRPPEILLG STRYTKGVDM WSVGCILGEL MLGKPIFPGR STTNQLELIC SVTGMPSAAD VASTNSQFAH AMLRDIHCAH RRTFAELLPG ASPDALDLIE RLMCFNPNKR MTAAEALEHP YVAAFHRPDE EPVAAEPITV SLPDSQRLPL AKYRDAIYEQ IAALRRSSTS TDQRQRAERQ AAGSTASRKT SVSSTTASAA PGGSRAGTST SGVTRPTNSS SGSAAARAAL QRGVVKSSST STVNEPSSKL YGRPAFRSAT SATSGLESRP VAREAAVRK
NT Sequence
ATGTCGACTG AAATCGAGTC TCATATTCTG AAGAAGTATG AGATTCAGAC ACAGCTGGGA CAGGGTGCGT ACGGCATTGT TTGGCGCGCC CTGGAGCGCA AACACAACCG TGTTGTTGCA CTCAAGAAGA TTTACGATGC CTTCCAAAAC TCGACCGATG CGCAGCGTAC CTTCCGAGAG ATCATGTTCT TACACCGGTT GCACCACCCA AATATCATCA AGCTGCTGCA CGTTCATCGA GCCTTCAATG ACCGCGACAT ATACCTGGTT TTCGAATACA TGGAGACGGA CCTGCACGTG GTGATCAGGG CAAACATCTT GGAGGACATC CACAAACAGT TCATCATTTA CCAGCTGCTG AAGACGATGA AGTACCTGCA CTCTGCTGAG ATTCTTCACC GTGACATGAA ACCCAGCAAT CTGCTTGTCA ACAGTGACTG CACCATGAAG GTGGCTGACT TCGGTCTTGC ACGCTCCATT TTGTCCCTTG AGGGTGAGCA GGTGTCGCGA CCGGTGCTAA CGGACTATAT TGCGACACGG TGGTATCGCC CCCCGGAGAT TTTGCTCGGT TCGACACGAT ACACGAAGGG TGTGGATATG TGGTCTGTGG GCTGCATTCT TGGGGAGTTG ATGCTGGGAA AGCCCATCTT TCCTGGGCGC TCCACGACAA ACCAGTTGGA ACTAATTTGT AGCGTGACGG GGATGCCTTC CGCGGCGGAT GTGGCGTCGA CCAATTCGCA GTTTGCCCAC GCAATGCTCC GTGACATCCA CTGCGCTCAT CGACGCACTT TCGCCGAGCT GCTGCCAGGG GCTTCCCCCG ATGCATTGGA CCTAATCGAG CGCCTGATGT GTTTCAACCC GAACAAGCGC ATGACGGCTG CCGAGGCCCT GGAGCACCCG TATGTCGCTG CGTTTCACCG CCCTGACGAA GAGCCGGTGG CGGCAGAGCC CATTACCGTC TCTCTCCCCG ACAGCCAACG TCTGCCCTTG GCCAAGTACA GAGACGCTAT TTACGAGCAA ATCGCCGCGC TGCGCCGTAG CTCCACGTCC ACCGATCAGC GGCAGCGCGC GGAGCGGCAA GCCGCTGGCA GCACCGCTAG CCGAAAAACG TCCGTGAGCA GCACCACTGC CTCCGCCGCC CCTGGTGGCT CTCGAGCAGG CACCAGCACC TCTGGGGTCA CGCGACCCAC GAACAGCAGC AGCGGCAGCG CCGCCGCACG GGCGGCTCTG CAGCGGGGCG TTGTGAAGTC TAGCTCCACT AGTACGGTCA ACGAACCATC ATCTAAGTTG TATGGCCGCC CGGCCTTCCG ATCAGCAACA TCTGCTACCT CTGGTCTGGA AAGCAGGCCT GTCGCGCGTG AGGCAGCGGT TCGCAAATGA
Details for LetaA.01391.a.HZ3.GE43733
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS TEIESHILKK YEIQTQLGQG AYGIVWRALE RKHNRVVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAF NDRDIYLVFE YMETDLHVVI RANILEDIHK QFIIYQLLKT MKYLHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLARSILS LEGEQVSRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILGELML GKPIFPGRST TNQLELICSV TGMPSAADVA STNSQFAHAM LRDIHCAHRR TFAELLPGAS PDALDLIERL MCFNPNKRMT AAEALEHPYV AAFHRPDEEP VAAEPITVSL PDSQRLPLAK YRDAIYEQIA ALRRSSTSTD QRQRAER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtcgactga aatcgagtct catattctga agaagtatga gattcagaca cagctgggac agggtgcgta cggcattgtt tggcgcgccc tggagcgcaa acacaaccgt gttgttgcac tcaagaagat ttacgatgcc ttccaaaact cgaccgatgc gcagcgtacc ttccgagaga tcatgttctt acaccggttg caccacccaa atatcatcaa gctgctgcac gttcatcgag ccttcaatga ccgcgacata tacctggttt tcgaatacat ggagacggac ctgcacgtgg tgatcagggc aaacatcttg gaggacatcc acaaacagtt catcatttac cagctgctga agacgatgaa gtacctgcac tctgctgaga ttcttcaccg tgacatgaaa cccagcaatc tgcttgtcaa cagtgactgc accatgaagg tggctgactt cggtcttgca cgctccattt tgtcccttga gggtgagcag gtgtcgcgac cggtgctaac ggactatatt gcgacacggt ggtatcgccc cccggagatt ttgctcggtt cgacacgata cacgaagggt gtggatatgt ggtctgtggg ctgcattctt ggggagttga tgctgggaaa gcccatcttt cctgggcgct ccacgacaaa ccagttggaa ctaatttgta gcgtgacggg gatgccttcc gcggcggatg tggcgtcgac caattcgcag tttgcccacg caatgctccg tgacatccac tgcgctcatc gacgcacttt cgccgagctg ctgccagggg cttcccccga tgcattggac ctaatcgagc gcctgatgtg tttcaacccg aacaagcgca tgacggctgc cgaggccctg gagcacccgt atgtcgctgc gtttcaccgc cctgacgaag agccggtggc ggcagagccc attaccgtct ctctccccga cagccaacgt ctgcccttgg ccaagtacag agacgctatt tacgagcaaa tcgccgcgct gcgccgtagc tccacgtcca ccgatcagcg gcagcgcgcg gagcggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa