SSGCID: Target Page

LetaA.01238.a

Seryl-tRNA synthetase, putative

CENTER ID:	LetaA.01238.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.01238.a.AE1.GE42993	full length		1	474

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.01238.a.AE1.PW38624	full length		1	474

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP11.0110

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLDIQLFRD PQTAVIVRES ERRRYAKAEV VDEIIDIDKR WRRTQFLTEA SKKMINTCSK AVGAKKKAKE ADGDVSEVPV DVMNAAQEGT LDTKKLESLC ILQLKELSKA LATQVESLAK MAAEQEAERD RVLASVGNVL HESVPVSNDE ETGNVVVRTF GDVTRRMKMT HVDCMERLGL MDTSKTVTGM AGGRAFVLRG GLVQLQFALI SYALNFLIAR GYEPFYPPFF LNKEYMGAVA QLSDFDESLY KVSGDGDEKY LIATSEAPIA AYHTNKWFTE MKEPIKYAGV SSCFRKEAGA HGRDTLGIFR VHQFDKIEQF VVCSPREDQS WKMLEEMIQT SQDFNESLGL PYRVINICSG ALNNAAAKKY DLEAWFPGSG AFRELVSCSN CTDYQARSVN CRFGPNTKGS TANNTKEYCH MLNGTLCAVT RTMCCICENY QTEEGIVIPE VLRPFMMGTE ILKFPAEAPV ADKE

NT Sequence

ATGGGGCTCG ACATCCAGCT GTTCCGCGAT CCCCAGACAG CGGTGATAGT GCGCGAGTCG GAGCGCCGCC GCTACGCCAA GGCGGAGGTC GTCGATGAAA TCATAGACAT TGACAAGCGC TGGCGCCGCA CCCAGTTTCT CACGGAAGCG AGTAAGAAGA TGATAAACAC TTGCAGCAAG GCGGTCGGTG CTAAGAAGAA AGCAAAGGAA GCGGACGGCG ACGTGTCTGA GGTGCCTGTA GATGTCATGA ATGCCGCCCA GGAAGGCACA CTGGACACGA AGAAGCTGGA ATCATTGTGC ATCCTTCAGC TAAAGGAGTT GTCCAAGGCA CTGGCGACGC AGGTGGAGAG TCTCGCAAAG ATGGCTGCGG AGCAGGAAGC GGAGCGGGAT CGCGTGCTGG CCAGCGTTGG CAACGTTCTG CACGAGTCCG TCCCAGTCTC AAACGATGAG GAAACGGGAA ACGTGGTCGT GCGCACCTTT GGTGACGTGA CGCGCCGCAT GAAGATGACA CATGTAGATT GCATGGAGCG TCTGGGACTT ATGGACACGT CGAAGACAGT GACCGGGATG GCGGGTGGTC GCGCGTTTGT TCTTCGCGGT GGTCTTGTGC AACTGCAGTT CGCCCTTATC TCATACGCGC TCAACTTCCT TATCGCCCGC GGCTACGAGC CCTTCTACCC GCCCTTCTTC CTCAACAAGG AATATATGGG TGCTGTGGCG CAGCTTTCTG ACTTTGACGA GTCTCTCTAC AAAGTATCCG GCGACGGGGA CGAGAAGTAT CTGATTGCGA CGAGCGAGGC ACCGATTGCC GCCTACCACA CCAACAAGTG GTTTACGGAG ATGAAGGAAC CGATCAAGTA CGCGGGTGTC TCGTCGTGCT TCCGAAAGGA AGCGGGTGCG CACGGTCGTG ACACACTTGG CATTTTCCGC GTTCACCAGT TCGACAAGAT TGAGCAGTTT GTGGTGTGCT CTCCTCGCGA AGATCAGTCG TGGAAGATGC TGGAAGAAAT GATCCAGACC TCGCAGGACT TCAATGAAAG CCTCGGTCTG CCGTACCGCG TCATCAATAT CTGCTCTGGT GCCCTCAACA ACGCCGCTGC GAAAAAGTAT GATCTGGAGG CATGGTTCCC CGGCTCTGGC GCATTCCGTG AGCTTGTTTC GTGCTCAAAC TGCACGGACT ACCAGGCCCG CAGCGTTAAC TGCCGCTTCG GCCCCAACAC AAAGGGAAGC ACAGCGAACA ACACGAAGGA GTACTGTCAC ATGTTAAACG GCACTCTCTG CGCTGTCACC CGCACAATGT GCTGTATCTG CGAAAACTAC CAGACCGAGG AAGGCATCGT GATCCCCGAG GTACTACGGC CTTTTATGAT GGGGACGGAG ATTCTCAAGT TCCCTGCAGA AGCCCCTGTA GCCGATAAGG AATAA

Details for LetaA.01238.a.AE1.GE42993

HARVESTED ON:	3/4/2019
SEQUENCED ON:	3/12/2019
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggggctcgac atccagctgt tccgcgatcc ccagacagcg gtgatagtgc gcgagtcgga gcgccgccgc tacgccaagg cggaggtcgt cgatgaaatc atagacattg acaagcgctg gcgccgcacc cagtttctca cggaagcgag taagaagatg ataaacactt gcagcaaggc ggtcggtgct aagaagaaag caaaggaagc ggacggcgac gtgtctgagg tgcctgtaga tgtcatgaat gccgcccagg aaggcacact ggacacgaag aagctggaat cattgtgcat ccttcagcta aaggagttgt ccaaggcact ggcgacgcag gtggagagtc tcgcaaagat ggctgcggag caggaagcgg agcgggatcg cgtgctggcc agcgttggca acgttctgca cgagtccgtc ccagtctcaa acgatgagga aacgggaaac gtggtcgtgc gcacctttgg tgacgtgacg cgccgcatga agatgacaca tgtagattgc atggagcgtc tgggacttat ggacacgtcg aagacagtga ccgggatggc gggtggtcgc gcgtttgttc ttcgcggtgg tcttgtgcaa ctgcagttcg cccttatctc atacgcgctc aacttcctta tcgcccgcgg ctacgagccc ttctacccgc ccttcttcct caacaaggaa tatatgggtg ctgtggcgca gctttctgac tttgacgagt ctctctacaa agtatccggc gacggggacg agaagtatct gattgcgacg agcgaggcac cgattgccgc ctaccacacc aacaagtggt ttacggagat gaaggaaccg atcaagtacg cgggtgtctc gtcgtgcttc cgaaaggaag cgggtgcgca cggtcgtgac acacttggca ttttccgcgt tcaccagttc gacaagattg agcagtttgt ggtgtgctct cctcgcgaag atcagtcgtg gaagatgctg gaagaaatga tccagacctc gcaggacttc aatgaaagcc tcggtctgcc gtaccgcgtc atcaatatct gctctggtgc cctcaacaac gccgctgcga aaaagtatga tctggaggca tggttccccg gctctggcgc attccgtgag cttgtttcgt gctcaaactg cacggactac caggcccgca gcgttaactg ccgcttcggc cccaacacaa agggaagcac agcgaacaac acgaaggagt actgtcacat gttaaacggc actctctgcg ctgtcacccg cacaatgtgc tgtatctgcg aaaactacca gaccgaggaa ggcatcgtga tccccgaggt actacggcct tttatgatgg ggacggagat tctcaagttc cctgcagaag cccctgtagc cgataaggaa gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat

Details for LetaA.01238.a.AE1.PW38624

PURIFICATION DATe:	6/4/2019
CONCENTRATION:	11mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GGGGCTCGAC ATCCAGCTGT TCCGCGATCC CCAGACAGCG GTGATAGTGC GCGAGTCGGA GCGCCGCCGC TACGCCAAGG CGGAGGTCGT CGATGAAATC ATAGACATTG ACAAGCGCTG GCGCCGCACC CAGTTTCTCA CGGAAGCGAG TAAGAAGATG ATAAACACTT GCAGCAAGGC GGTCGGTGCT AAGAAGAAAG CAAAGGAAGC GGACGGCGAC GTGTCTGAGG TGCCTGTAGA TGTCATGAAT GCCGCCCAGG AAGGCACACT GGACACGAAG AAGCTGGAAT CATTGTGCAT CCTTCAGCTA AAGGAGTTGT CCAAGGCACT GGCGACGCAG GTGGAGAGTC TCGCAAAGAT GGCTGCGGAG CAGGAAGCGG AGCGGGATCG CGTGCTGGCC AGCGTTGGCA ACGTTCTGCA CGAGTCCGTC CCAGTCTCAA ACGATGAGGA AACGGGAAAC GTGGTCGTGC GCACCTTTGG TGACGTGACG CGCCGCATGA AGATGACACA TGTAGATTGC ATGGAGCGTC TGGGACTTAT GGACACGTCG AAGACAGTGA CCGGGATGGC GGGTGGTCGC GCGTTTGTTC TTCGCGGTGG TCTTGTGCAA CTGCAGTTCG CCCTTATCTC ATACGCGCTC AACTTCCTTA TCGCCCGCGG CTACGAGCCC TTCTACCCGC CCTTCTTCCT CAACAAGGAA TATATGGGTG CTGTGGCGCA GCTTTCTGAC TTTGACGAGT CTCTCTACAA AGTATCCGGC GACGGGGACG AGAAGTATCT GATTGCGACG AGCGAGGCAC CGATTGCCGC CTACCACACC AACAAGTGGT TTACGGAGAT GAAGGAACCG ATCAAGTACG CGGGTGTCTC GTCGTGCTTC CGAAAGGAAG CGGGTGCGCA CGGTCGTGAC ACACTTGGCA TTTTCCGCGT TCACCAGTTC GACAAGATTG AGCAGTTTGT GGTGTGCTCT CCTCGCGAAG ATCAGTCGTG GAAGATGCTG GAAGAAATGA TCCAGACCTC GCAGGACTTC AATGAAAGCC TCGGTCTGCC GTACCGCGTC ATCAATATCT GCTCTGGTGC CCTCAACAAC GCCGCTGCGA AAAAGTATGA TCTGGAGGCA TGGTTCCCCG GCTCTGGCGC ATTCCGTGAG CTTGTTTCGT GCTCAAACTG CACGGACTAC CAGGCCCGCA GCGTTAACTG CCGCTTCGGC CCCAACACAA AGGGAAGCAC AGCGAACAAC ACGAAGGAGT ACTGTCACAT GTTAAACGGC ACTCTCTGCG CTGTCACCCG CACAATGTGC TGTATCTGCG AAAACTACCA GACCGAGGAA GGCATCGTGA TCCCCGAGGT ACTACGGCCT TTTATGATGG GGACGGAGAT TCTCAAGTTC CCTGCAGAAG CCCCTGTAGC CGATAAGGAA GGGCACCACC ATCATCATCA TTAACGGATC CGAATTCGAG CTCCGTCGAC AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT