LetaA.01169.a

protein phosphatase 1 catalytic subunit, putative (PP1C)

CENTER ID: LetaA.01169.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.01169.a.B1.GE42329 full length 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP15.0230

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASTKRGRFT MDLSMCTLSS LPTDTLSVEK ENDVLYRLLE LLPERYTSKV LEEQRIPEGL IVQILIKARA IIASEPMLIE LDSPVYTCGD LHGQYYDLVN IFKRCPPLGG TVFGTDAKKA RKENNSVIDD TTYSFLFLGD YVDRGARSVE VVVTLLALKI ISPRHITMLR GNHEDEQIML LYGFYDECKR RYGIKLFKMF SDLFRVLPVA ALVNGSIFCV HGGLSAELHV VRDIPDTRPC NVPHSGVICD LLWADPETDL PAGVDWAPSS RRISSVFSER ALERFLVEND IDLVCRAHQV VEEGFQFFPD HKKRHLLTIF SATNYCNEFG NRGGILRVDE KGVCSIITLE PPDFVQQRDI MLLRDPLPNP LSHD
NT Sequence
atggcaagca cgaaaagagg gagattcact atggatctgt ccatgtgtac tctcagcagc cttccaacag atactctctc cgtggaaaaa gagaatgatg tgctttatcg ccttttagaa cttcttccgg agcgctacac gagcaaagtt ttggaagagc agcgaattcc tgaaggtctc attgtgcaga ttctcatcaa ggcacgtgct ataatagcta gtgagccaat gcttatcgag ctagattccc ctgtttacac ttgcggcgat ctacacgggc agtactacga cttggttaac attttcaaac gatgcccgcc actaggaggt acggttttcg gaactgatgc gaagaaggca cgtaaagaaa ataactcagt catcgatgat acaacataca gcttcctctt ccttggcgac tatgtcgacc gcggcgctcg ttccgttgaa gtcgttgtga cacttctcgc tctcaagatt atttctccgc gacacatcac tatgcttcgc ggtaaccatg aggacgaaca aattatgctc ttgtatggat tctatgacga atgtaagaga cgctatggca ttaaactttt caaaatgttc tctgatcttt ttcgtgttct gcctgtggca gccttggtca acggctctat tttctgcgtc cacggaggtc tttccgcaga actccatgtc gtacgagaca tcccggacac gcgtccgtgc aacgttccgc actccggtgt tatttgcgac cttctgtggg ccgatcccga aacggatttg ccagcgggtg tagactgggc cccgagctcg cgcagaattt catctgtgtt ttccgagcgt gcactcgagc ggtttttagt ggaaaatgac attgatttag tgtgccgtgc acatcaagtg gtcgaagaag gatttcagtt tttcccggat cacaaaaaga ggcatttgct taccatcttc tccgctacta actactgcaa cgagtttgga aatagaggtg gcattttacg tgtggatgag aaaggtgtgt gtagcattat cacattggag ccaccggact ttgttcagca gcgggatatc atgcttttac gggacccact tccaaatcct ttgagccacg actga
Details for LetaA.01169.a.B1.GE42329
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Validated AA Sequence
MLRGNHEDEQ IMLLYGFYDE CKRRYGIKLF KMFSDLFRVL PVAALVNGSI FCVHGGLSAE LHVVRDIPDT RPCNVPHSGV ICDLLWADPE TDLPAGVDWA PSSRRISSVF SERALERFLV ENDIDLVCRA HQVVEEGFQF FPDHKKRHLL TIFSATNYCN EFGNRGGILR VDEKGVCSII TLEPPDFVQQ RDIMLLRD
Validated NT Sequence
gggtcccgta aaagcatgat atcccgctgc tgaacaaagt ccggtggctc caatgtgata atgctacaca cacctttctc atccacacgt aaaatgccac ctctatttcc aaactcgttg cagtagttag tagcggagaa gatggtaagc aaatgcctct ttttgtgatc cgggaaaaac tgaaatcctt cttcgaccac ttgatgtgca cggcacacta aatcaatgtc attttccact aaaaaccgct cgagtgcacg ctcggaaaac acagatgaaa ttctgcgcga gctcggggcc cagtctacac ccgctggcaa atccgtttcg ggatcggccc acagaaggtc gcaaataaca ccggagtgcg gaacgttgca cggacgcgtg tccgggatgt ctcgtacgac atggagttct gcggaaagac ctccgtggac gcagaaaata gagccgttga ccaaggctgc cacaggcaga acacgaaaaa gatcagagaa cattttgaaa agtttaatgc catagcgtct cttacattcg tcatagaatc catacaagag cataatttgt tcgtcctcat ggttaccgcg aagcatagtg atgtgtcgcg gagaaataat cttgagagcg agaagtgtca caacgacttc aacggaacga gcgccgcggt cgacatagtc gccaaggaag aggaagctgt atgttgtntc atcgatgact gagctatttt ctttacgtgc cttcttcgca tcnnnnnnna aaaccgnncc tcnnnntggc gggcatnnnt ngaaaatgtt
Expected Protein Sequence
MAHHHHHHMA STKRGRFTMD LSMCTLSSLP TDTLSVEKEN DVLYRLLELL PERYTSKVLE EQRIPEGLIV QILIKARAII ASEPMLIELD SPVYTCGDLH GQYYDLVNIF KRCPPLGGTV FGTDAKKARK ENNSVIDDTT YSFLFLGDYV DRGARSVEVV VTLLALKIIS PRHITMLRGN HEDEQIMLLY GFYDECKRRY GIKLFKMFSD LFRVLPVAAL VNGSIFCVHG GLSAELHVVR DIPDTRPCNV PHSGVICDLL WADPETDLPA GVDWAPSSRR ISSVFSERAL ERFLVENDID LVCRAHQVVE EGFQFFPDHK KRHLLTIFSA TNYCNEFGNR GGILRVDEKG VCSIITLEPP DFVQQRDIML LRDPLPNPLS HD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa gcacgaaaag agggagattc actatggatc tgtccatgtg tactctcagc agccttccaa cagatactct ctccgtggaa aaagagaatg atgtgcttta tcgcctttta gaacttcttc cggagcgcta cacgagcaaa gttttggaag agcagcgaat tcctgaaggt ctcattgtgc agattctcat caaggcacgt gctataatag ctagtgagcc aatgcttatc gagctagatt cccctgttta cacttgcggc gatctacacg ggcagtacta cgacttggtt aacattttca aacgatgccc gccactagga ggtacggttt tcggaactga tgcgaagaag gcacgtaaag aaaataactc agtcatcgat gatacaacat acagcttcct cttccttggc gactatgtcg accgcggcgc tcgttccgtt gaagtcgttg tgacacttct cgctctcaag attatttctc cgcgacacat cactatgctt cgcggtaacc atgaggacga acaaattatg ctcttgtatg gattctatga cgaatgtaag agacgctatg gcattaaact tttcaaaatg ttctctgatc tttttcgtgt tctgcctgtg gcagccttgg tcaacggctc tattttctgc gtccacggag gtctttccgc agaactccat gtcgtacgag acatcccgga cacgcgtccg tgcaacgttc cgcactccgg tgttatttgc gaccttctgt gggccgatcc cgaaacggat ttgccagcgg gtgtagactg ggccccgagc tcgcgcagaa tttcatctgt gttttccgag cgtgcactcg agcggttttt agtggaaaat gacattgatt tagtgtgccg tgcacatcaa gtggtcgaag aaggatttca gtttttcccg gatcacaaaa agaggcattt gcttaccatc ttctccgcta ctaactactg caacgagttt ggaaatagag gtggcatttt acgtgtggat gagaaaggtg tgtgtagcat tatcacattg gagccaccgg actttgttca gcagcgggat atcatgcttt tacgggaccc acttccaaat cctttgagcc acgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat