LetaA.01010.a

Mitogen-activated protein kinase kinase 3, putative

CENTER ID: LetaA.01010.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.01010.a.HZ2.GE43732 kinase domain (Jensen) 22 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP17.0060

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFYALQDSL FCNAKSGGSS DTVDVESRYI LLEKIGSGSY GDVWSATRRT GGKDMYAVKR INKRRAGTKG LRSVMGEVET MSLLSHPNIV KLEETYQDEA CLSIVMEYLP GGSLQHRIDR QNVSEQEVRR FITQLLMAVE YIHEKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVFAGNK QCLTTCCGTL HFMAPEILLE KNYGKLVDMW AMGVMAHVMF FGSYPFQART EAALKNEICR GYQPPAEGGG GLRCPPQLQD FISQLLLYDP HRRMSAKDAL KHPWIKEGLE ANRRTSLYAS ESPLLTAMRP SARWRSAVLA VMVVQRLLYL QKIQRLVRLG FDGFPILRDY RYLVTGKYVP ASTSLECSHM FHSRPMALLE LISMIDTCPT LKHVDISWNN IHSLSVIQSL LKVVARHPSL QSIDLSHNPI PAVAGRSIVR LIRNPLSQVT CINVADTGIT ADTIGQINSF LREKLTPAAI SYGVSTGDTQ SRVTPGSCED LLLTSSSTTV SSLQQKRGIT SGPPKQEAKK EWRGVRLPPI SRPPASKRNS KMQ
NT Sequence
ATGGACTTCT ACGCTTTGCA AGACAGTCTC TTCTGCAATG CCAAGAGCGG AGGAAGTTCG GACACCGTCG ATGTCGAGTC TAGGTATATC TTGCTCGAGA AAATTGGCTC TGGGTCTTAT GGCGATGTCT GGAGCGCGAC TCGGCGCACA GGGGGGAAGG ACATGTACGC GGTGAAGCGC ATTAACAAGC GCCGGGCGGG CACCAAGGGA CTGCGGAGCG TCATGGGGGA AGTTGAGACG ATGAGTCTTC TTTCTCATCC TAACATCGTC AAGCTTGAGG AGACGTATCA GGACGAGGCG TGCCTTTCAA TTGTAATGGA GTACCTTCCT GGCGGTTCCC TTCAGCACCG CATAGACCGC CAAAACGTTT CTGAACAGGA GGTGCGACGC TTTATCACGC AGCTTTTGAT GGCGGTCGAG TATATTCATG AAAAAGGTAT TGTTCACCGA GATTTGAAGC CGAGCAACTG TCTACTATCT CAAAACGATC TCGTTGTGAA GATATCAGAC TTTGGACTCT CCGTGTTTGC TGGCAATAAG CAATGTTTGA CAACGTGTTG CGGCACACTG CACTTCATGG CACCCGAGAT TCTGCTAGAG AAGAACTACG GAAAGCTGGT GGACATGTGG GCTATGGGGG TCATGGCACA CGTGATGTTT TTCGGTAGTT ACCCATTTCA AGCCCGTACA GAGGCGGCTC TGAAGAACGA AATATGTCGC GGGTATCAGC CTCCGGCGGA GGGTGGTGGA GGGCTGCGTT GCCCACCGCA GCTCCAAGAC TTCATCAGTC AACTTCTCCT TTACGACCCA CATCGTCGAA TGAGCGCCAA AGACGCTCTT AAGCACCCGT GGATCAAGGA AGGCCTGGAG GCCAACCGGC GGACTTCCCT CTACGCATCG GAGTCTCCCT TGCTGACAGC GATGAGACCA TCTGCGCGGT GGCGGTCGGC AGTACTCGCC GTCATGGTAG TGCAGCGTCT GCTGTACCTG CAGAAAATTC AGCGGCTAGT GCGGTTGGGC TTCGACGGGT TTCCAATTTT GCGTGACTAC CGCTACCTTG TCACCGGCAA GTATGTGCCT GCTAGCACCT CTCTAGAGTG TAGCCACATG TTTCACTCGC GACCGATGGC GCTTCTCGAG TTGATTTCTA TGATTGATAC GTGCCCGACA CTGAAACACG TAGATATTAG TTGGAATAAC ATTCACAGCT TATCTGTAAT CCAGTCCCTT CTCAAAGTGG TAGCGCGACA TCCTTCGTTG CAGTCTATTG ACCTGTCTCA CAATCCTATT CCTGCTGTGG CAGGGCGTTC GATCGTGCGA TTAATCCGCA ACCCACTTTC ACAGGTAACG TGCATCAACG TCGCGGACAC CGGCATCACT GCCGACACCA TTGGGCAGAT TAACTCCTTT CTGAGGGAGA AGCTGACCCC TGCTGCGATA TCCTACGGCG TCTCCACAGG AGACACGCAA AGCCGAGTCA CTCCGGGCAG CTGCGAAGAC CTGCTCCTAA CATCATCCTC CACTACCGTA AGCTCCTTGC AGCAGAAGCG GGGCATTACT AGCGGACCAC CCAAGCAGGA AGCGAAGAAA GAATGGCGCG GTGTACGGCT TCCGCCAATT TCCCGCCCAC CGGCGTCGAA GCGGAATTCG AAAATGCAGT GA
Details for LetaA.01010.a.HZ2.GE43732
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 61
Validated AA Sequence
MGXVETMSLL SHPNIVKLEE TYQDEACLSI VMEYXPGGXL QHRIDRQNVS EQEVRRFITQ LLMAVEYIHE KGIVHRDLKP SNCLLSQNDL VVKISDFGLS VFAGNKQCLT TCCGTLHFMA PEILLEKNYG KLVDMWAMGV MAHVMFFGSY PFQARTEAAL KNEICRGYQP PAEGGGGLRC PPQLQDFISQ LLLYDPHRRM SAKDALKHPW IKEGLEANRR TSLYASESPL LTAMRPSARW RSAVLAVMVV QRLLYLQKIQ RLVRLGFDGF P
Validated NT Sequence
gttnagcagc cggatcctcg agaagcttgg ctgcagaact tgttcgtgct gtttattatg gaaacccgtc gaagcccaac cgcactagcc gctgaatttt ctgcaggtac agcagacgct gcactaccat gacggcgagt actgccgacc gccaccgcgc agatggtctc atcgctgtca gcaagggaga ctccgatgcg tagagggaag tccgccggtt ggcctccagg ccttccttga tccacgggtg cttaagagcg tctttggcgc tcattcgacg atgtgggtcg taaaggagaa gttgactgat gaagtcttgg agctgcggtg ggcaacgcag ccctccacca ccctccgccg gaggctgata cccgcgacat atttcgttct tcagagccgc ctctgtacgg gcttgaaatg ggtaactacc gaaaaacatc acgtgtgcca tgacccccat agcccacatg tccaccagct ttccgtagtt cttctctagc agaatctcgg gtgccatgaa gtgcagtgtg ccgcaacacg ttgtcaaaca ttgcttattg ccagcaaaca cggagagtcc aaagtctgat atcttcacaa cgagatcgtt ttgagatagt agacagttgc tcggcttcaa atctcggtga acaatacctt tttcatgaat atactcgacc gccatcaaaa gctgcgtgat aaagcgtcgc acctcctgtt cagaaacgtt ttggcggtct atgcggtgct gaaggnaacc gccaggaang tactccatta caattgaaag gcacgcctcg tcctgatacg tctcctcnag cttgacgatg ttaggatgag aaagaagact catcgtctca actnncccca tgacgctccg cagtcccttt ggtgcccgcc cnggngcttg ttaatgcgct tcaccgcgta nnngtccttn cccncctgtg cgccgannnn ngctcagaca tcgccntaag acccagagcn nattttnnnn cnagcaagaa nntnncc
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSTV DVESRYILLE KIGSGSYGDV WSATRRTGGK DMYAVKRINK RRAGTKGLRS VMGEVETMSL LSHPNIVKLE ETYQDEACLS IVMEYLPGGS LQHRIDRQNV SEQEVRRFIT QLLMAVEYIH EKGIVHRDLK PSNCLLSQND LVVKISDFGL SVFAGNKQCL TTCCGTLHFM APEILLEKNY GKLVDMWAMG VMAHVMFFGS YPFQARTEAA LKNEICRGYQ PPAEGGGGLR CPPQLQDFIS QLLLYDPHRR MSAKDALKHP WIKEGLEANR RTSLYASESP LLTAMRPSAR WRSAVLAVMV VQRLLYLQKI QRLVRLGFDG FP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga ccgtcgatgt cgagtctagg tatatcttgc tcgagaaaat tggctctggg tcttatggcg atgtctggag cgcgactcgg cgcacagggg ggaaggacat gtacgcggtg aagcgcatta acaagcgccg ggcgggcacc aagggactgc ggagcgtcat gggggaagtt gagacgatga gtcttctttc tcatcctaac atcgtcaagc ttgaggagac gtatcaggac gaggcgtgcc tttcaattgt aatggagtac cttcctggcg gttcccttca gcaccgcata gaccgccaaa acgtttctga acaggaggtg cgacgcttta tcacgcagct tttgatggcg gtcgagtata ttcatgaaaa aggtattgtt caccgagatt tgaagccgag caactgtcta ctatctcaaa acgatctcgt tgtgaagata tcagactttg gactctccgt gtttgctggc aataagcaat gtttgacaac gtgttgcggc acactgcact tcatggcacc cgagattctg ctagagaaga actacggaaa gctggtggac atgtgggcta tgggggtcat ggcacacgtg atgtttttcg gtagttaccc atttcaagcc cgtacagagg cggctctgaa gaacgaaata tgtcgcgggt atcagcctcc ggcggagggt ggtggagggc tgcgttgccc accgcagctc caagacttca tcagtcaact tctcctttac gacccacatc gtcgaatgag cgccaaagac gctcttaagc acccgtggat caaggaaggc ctggaggcca accggcggac ttccctctac gcatcggagt ctcccttgct gacagcgatg agaccatctg cgcggtggcg gtcggcagta ctcgccgtca tggtagtgca gcgtctgctg tacctgcaga aaattcagcg gctagtgcgg ttgggcttcg acgggtttcc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa