SSGCID: Target Page

LetaA.00825.c

Aspartyl-tRNA synthetase, putative

CENTER ID:	LetaA.00825.c
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00825.c.B1.GE42554	full length		1	549

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00825.c.B1.PW38619	full length		1	549

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP30.0530

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNPNPADASA PAVKKMSDKE ARKAARLAEE KARAEEKAAL VEKYKDVFGA APMVQSTTYK SRTHIPVSEL SDPALVGKTV LIRARVSTTR KKGKMAFMVL RDGSDSVQAM AAVEGDVPKE MIDFMGQIST ESIVDVEATV CKVEQPITST SHSDVELKVK KIHTVTESLR TLPFTLEDAS RKESDEGAKV NLDTRLSSRW MDLRTPASGA IFRLQSRVCQ YFRQFLIDND FCEIHSPKII NAPSEGGANV FKLEYFNRFA YLAQSPQLYK QMVLQGDVPR VFEVGPVFRS ENSNTHRHLT EFVGLDVEMR IDEHYYEVLD VAENLFNYMF EHLSTHTKEL KSVCQQYPFE PLVWKLTPEK MKELGVGIIS EGVEPTDKFQ ARVHNMDSRM LRINYMHCIE LLNTVLDEKM EPTDDINTTN EKLLGKLVKE RYGTDFFISD RFPSSVRPFY TMECKDDVRF TNSYDMFIRG EEISSGAQRI HDPDMLLARA KVLNVDLTPI KEYVDSFRLG AWPHGGFGVG LERVVMLYLG LSNVRLASLF PRDPQRTTP

NT Sequence

ATGAACCCCA ACCCCGCTGA TGCCAGCGCA CCAGCCGTCA AGAAGATGAG CGACAAGGAG GCTCGAAAGG CGGCCCGCCT GGCTGAGGAG AAGGCCCGTG CGGAGGAAAA GGCTGCCCTT GTGGAGAAGT ACAAGGATGT GTTTGGTGCT GCACCAATGG TGCAGTCGAC GACGTACAAG TCGCGCACGC ACATCCCTGT CTCGGAGCTG TCAGATCCGG CGTTGGTGGG GAAGACGGTA TTGATCCGTG CCCGCGTCTC GACGACGCGC AAGAAGGGCA AGATGGCGTT CATGGTGCTG CGCGATGGGA GCGACTCGGT GCAGGCGATG GCCGCCGTAG AGGGTGACGT GCCAAAGGAG ATGATCGACT TCATGGGACA GATCTCGACA GAGTCCATCG TTGACGTGGA GGCGACCGTG TGCAAGGTGG AGCAGCCTAT CACGTCGACG TCCCACTCGG ATGTGGAGCT GAAAGTGAAG AAGATTCACA CGGTGACAGA GTCGCTGCGT ACATTGCCGT TCACGCTAGA GGACGCGAGC CGCAAGGAGT CGGACGAGGG CGCGAAGGTG AACCTTGACA CGCGCTTGAG TAGCCGCTGG ATGGACCTGC GCACGCCGGC GTCGGGCGCT ATTTTCCGCC TTCAGTCGCG CGTGTGCCAG TACTTCCGCC AGTTTCTTAT CGACAACGAC TTCTGTGAGA TTCACTCGCC CAAAATCATC AACGCACCGA GCGAGGGGGG CGCCAACGTG TTCAAGCTGG AGTACTTCAA CCGCTTTGCG TATCTTGCAC AGTCACCACA GCTGTACAAG CAGATGGTGC TCCAGGGCGA TGTGCCACGT GTGTTTGAGG TGGGGCCTGT GTTTCGCTCC GAAAACAGCA ACACACATCG CCACTTGACA GAGTTTGTTG GGTTGGATGT GGAGATGCGC ATCGATGAAC ACTACTACGA AGTGCTGGAT GTGGCCGAGA ACCTTTTCAA CTACATGTTC GAGCACCTTT CCACTCACAC AAAGGAGCTG AAGAGCGTGT GCCAGCAGTA CCCCTTCGAG CCTCTTGTGT GGAAGCTCAC GCCAGAGAAG ATGAAGGAGC TCGGCGTTGG CATCATCTCG GAGGGCGTGG AGCCGACAGA CAAGTTCCAG GCACGCGTGC ATAACATGGA TAGTCGTATG CTGCGCATCA ACTACATGCA CTGCATAGAG CTGCTGAACA CTGTGCTGGA CGAGAAGATG GAGCCGACTG ATGATATCAA CACGACGAAC GAGAAGCTTC TCGGCAAGCT TGTGAAAGAG CGCTACGGCA CCGACTTCTT CATCTCGGAT CGCTTCCCGT CTTCGGTGCG GCCGTTCTAC ACGATGGAGT GCAAGGACGA CGTGCGCTTC ACGAACTCAT ACGATATGTT CATTCGAGGT GAGGAGATCT CCAGTGGAGC GCAGCGCATA CACGACCCCG ATATGCTGCT GGCGCGCGCC AAGGTACTGA ACGTGGACCT CACGCCGATC AAGGAGTACG TTGACTCGTT CCGTCTCGGT GCATGGCCGC ATGGCGGTTT TGGTGTTGGG CTGGAACGTG TGGTGATGCT GTACCTTGGA CTCAGCAACG TGCGCCTTGC TTCGCTCTTT CCGCGTGACC CCCAGCGCAC GACGCCGTAG

Details for LetaA.00825.c.B1.GE42554

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/11/2018
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN PNPADASAPA VKKMSDKEAR KAARLAEEKA RAEEKAALVE KYKDVFGAAP MVQSTTYKSR THIPVSELSD PALVGKTVLI RARVSTTRKK GKMAFMVLRD GSDSVQAMAA VEGDVPKEMI DFMGQISTES IVDVEATVCK VEQPITSTSH SDVELKVKKI HTVTESLRTL PFTLEDASRK ESDEGAKVNL DTRLSSRWMD LRTPASGAIF RLQSRVCQYF RQFLIDNDFC EIHSPKIINA PSEGGANVFK LEYFNRFAYL AQSPQLYKQM VLQGDVPRVF EVGPVFRSEN SNTHRHLTEF VGLDVEMRID EHYYEVLDVA ENLFNYMFEH LSTHTKELKS VCQQYPFEPL VWKLTPEKMK ELGVGIISEG VEPTDKFQAR VHNMDSRMLR INYMHCIELL NTVLDEKMEP TDDINTTNEK LLGKLVKERY GTDFFISDRF PSSVRPFYTM ECKDDVRFTN SYDMFIRGEE ISSGAQRIHD PDMLLARAKV LNVDLTPIKE YVDSFRLGAW PHGGFGVGLE RVVMLYLGLS NVRLASLFPR DPQRTTP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc ccaaccccgc tgatgccagc gcaccagccg tcaagaagat gagcgacaag gaggctcgaa aggcggcccg cctggctgag gagaaggccc gtgcggagga aaaggctgcc cttgtggaga agtacaagga tgtgtttggt gctgcaccaa tggtgcagtc gacgacgtac aagtcgcgca cgcacatccc tgtctcggag ctgtcagatc cggcgttggt ggggaagacg gtattgatcc gtgcccgcgt ctcgacgacg cgcaagaagg gcaagatggc gttcatggtg ctgcgcgatg ggagcgactc ggtgcaggcg atggccgccg tagagggtga cgtgccaaag gagatgatcg acttcatggg acagatctcg acagagtcca tcgttgacgt ggaggcgacc gtgtgcaagg tggagcagcc tatcacgtcg acgtcccact cggatgtgga gctgaaagtg aagaagattc acacggtgac agagtcgctg cgtacattgc cgttcacgct agaggacgcg agccgcaagg agtcggacga gggcgcgaag gtgaaccttg acacgcgctt gagtagccgc tggatggacc tgcgcacgcc ggcgtcgggc gctattttcc gccttcagtc gcgcgtgtgc cagtacttcc gccagtttct tatcgacaac gacttctgtg agattcactc gcccaaaatc atcaacgcac cgagcgaggg gggcgccaac gtgttcaagc tggagtactt caaccgcttt gcgtatcttg cacagtcacc acagctgtac aagcagatgg tgctccaggg cgatgtgcca cgtgtgtttg aggtggggcc tgtgtttcgc tccgaaaaca gcaacacaca tcgccacttg acagagtttg ttgggttgga tgtggagatg cgcatcgatg aacactacta cgaagtgctg gatgtggccg agaacctttt caactacatg ttcgagcacc tttccactca cacaaaggag ctgaagagcg tgtgccagca gtaccccttc gagcctcttg tgtggaagct cacgccagag aagatgaagg agctcggcgt tggcatcatc tcggagggcg tggagccgac agacaagttc caggcacgcg tgcataacat ggatagtcgt atgctgcgca tcaactacat gcactgcata gagctgctga acactgtgct ggacgagaag atggagccga ctgatgatat caacacgacg aacgagaagc ttctcggcaa gcttgtgaaa gagcgctacg gcaccgactt cttcatctcg gatcgcttcc cgtcttcggt gcggccgttc tacacgatgg agtgcaagga cgacgtgcgc ttcacgaact catacgatat gttcattcga ggtgaggaga tctccagtgg agcgcagcgc atacacgacc ccgatatgct gctggcgcgc gccaaggtac tgaacgtgga cctcacgccg atcaaggagt acgttgactc gttccgtctc ggtgcatggc cgcatggcgg ttttggtgtt gggctggaac gtgtggtgat gctgtacctt ggactcagca acgtgcgcct tgcttcgctc tttccgcgtg acccccagcg cacgacgccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for LetaA.00825.c.B1.PW38619

PURIFICATION DATe:	5/16/2019
CONCENTRATION:	10.5mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	10µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CCAACCCCGC TGATGCCAGC GCACCAGCCG TCAAGAAGAT GAGCGACAAG GAGGCTCGAA AGGCGGCCCG CCTGGCTGAG GAGAAGGCCC GTGCGGAGGA AAAGGCTGCC CTTGTGGAGA AGTACAAGGA TGTGTTTGGT GCTGCACCAA TGGTGCAGTC GACGACGTAC AAGTCGCGCA CGCACATCCC TGTCTCGGAG CTGTCAGATC CGGCGTTGGT GGGGAAGACG GTATTGATCC GTGCCCGCGT CTCGACGACG CGCAAGAAGG GCAAGATGGC GTTCATGGTG CTGCGCGATG GGAGCGACTC GGTGCAGGCG ATGGCCGCCG TAGAGGGTGA CGTGCCAAAG GAGATGATCG ACTTCATGGG ACAGATCTCG ACAGAGTCCA TCGTTGACGT GGAGGCGACC GTGTGCAAGG TGGAGCAGCC TATCACGTCG ACGTCCCACT CGGATGTGGA GCTGAAAGTG AAGAAGATTC ACACGGTGAC AGAGTCGCTG CGTACATTGC CGTTCACGCT AGAGGACGCG AGCCGCAAGG AGTCGGACGA GGGCGCGAAG GTGAACCTTG ACACGCGCTT GAGTAGCCGC TGGATGGACC TGCGCACGCC GGCGTCGGGC GCTATTTTCC GCCTTCAGTC GCGCGTGTGC CAGTACTTCC GCCAGTTTCT TATCGACAAC GACTTCTGTG AGATTCACTC GCCCAAAATC ATCAACGCAC CGAGCGAGGG GGGCGCCAAC GTGTTCAAGC TGGAGTACTT CAACCGCTTT GCGTATCTTG CACAGTCACC ACAGCTGTAC AAGCAGATGG TGCTCCAGGG CGATGTGCCA CGTGTGTTTG AGGTGGGGCC TGTGTTTCGC TCCGAAAACA GCAACACACA TCGCCACTTG ACAGAGTTTG TTGGGTTGGA TGTGGAGATG CGCATCGATG AACACTACTA CGAAGTGCTG GATGTGGCCG AGAACCTTTT CAACTACATG TTCGAGCACC TTTCCACTCA CACAAAGGAG CTGAAGAGCG TGTGCCAGCA GTACCCCTTC GAGCCTCTTG TGTGGAAGCT CACGCCAGAG AAGATGAAGG AGCTCGGCGT TGGCATCATC TCGGAGGGCG TGGAGCCGAC AGACAAGTTC CAGGCACGCG TGCATAACAT GGATAGTCGT ATGCTGCGCA TCAACTACAT GCACTGCATA GAGCTGCTGA ACACTGTGCT GGACGAGAAG ATGGAGCCGA CTGATGATAT CAACACGACG AACGAGAAGC TTCTCGGCAA GCTTGTGAAA GAGCGCTACG GCACCGACTT CTTCATCTCG GATCGCTTCC CGTCTTCGGT GCGGCCGTTC TACACGATGG AGTGCAAGGA CGACGTGCGC TTCACGAACT CATACGATAT GTTCATTCGA GGTGAGGAGA TCTCCAGTGG AGCGCAGCGC ATACACGACC CCGATATGCT GCTGGCGCGC GCCAAGGTAC TGAACGTGGA CCTCACGCCG ATCAAGGAGT ACGTTGACTC GTTCCGTCTC GGTGCATGGC CGCATGGCGG TTTTGGTGTT GGGCTGGAAC GTGTGGTGAT GCTGTACCTT GGACTCAGCA ACGTGCGCCT TGCTTCGCTC TTTCCGCGTG ACCCCCAGCG CACGACGCCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT