LetaA.00825.a

Lysyl-tRNA synthetase, putative

CENTER ID: LetaA.00825.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.00825.a.B1.GE42552 full length 1 586
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.00825.a.B1.PW38563 full length 1 586

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP15.0260

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPEELRKQ IEAIASQIAD TKKTKGVDCD EYKSLVKQMA TLRSQLPQDE KKLKKDKTVE PSYFESRLAM VKEMGLLGAA YPHKYHRQYT IPQYRRKYAP LLTEPDTSLN ETVTIAGRII NKRSSGSKLH FITIQGDMET VQVISSITDY IDQSKFPEIH SKLKRGDIIG IAGKPSLSKS SEFSLKATEI TLLSTCYHML PDDYFGLSSF EQRFRQRYLD FIVNRDNIKT FIQRANIIKY IRKFFDERDF VEVETPVLNQ IAGGAAARPF VTHHNDLNQT MFLRIAPELY LKELIVGGMD RVYEIGKQFR NEGIDLTHNP EFTSCEAYWA YMDYHDWMAT TEDLLYGLAM ELHGSPIVRY APKDSEGNPL PETTFNFSKP FKRLHIIPEL EKRLQVSFDN VDFESDAGTQ FLMDLCKKHK ADCPPPYTAA RLLDALIAEF LEPECHDPCF ICDHPRVMSP LAKWHRNDPR LTERFELFVN KKELANAYTE LNNPIVQREE FVKQVRNRDK GDDESMEIDE GFVAALEHAL PPTGGWGLGI DRLVMFLTSQ SNIKEVLLFP AMKPEGKNAI SYPPGTMLNG QGVPLL
NT Sequence
ATGTCCTCCC CCGAAGAGCT ACGTAAGCAA ATAGAGGCTA TTGCCTCTCA GATTGCAGAT ACCAAAAAAA CGAAGGGTGT TGACTGTGAT GAGTACAAGT CGCTGGTGAA GCAGATGGCT ACGCTACGGA GCCAGCTTCC GCAAGATGAG AAGAAGCTGA AGAAGGACAA GACGGTAGAG CCGTCATACT TCGAGTCACG ACTGGCGATG GTCAAGGAGA TGGGTCTCCT CGGTGCTGCC TATCCGCACA AGTACCACCG TCAGTACACA ATTCCACAGT ATCGCAGGAA GTACGCGCCT TTGCTCACAG AACCCGACAC CTCACTCAAC GAAACAGTCA CAATTGCTGG CCGCATTATC AATAAGCGTA GCTCGGGCTC AAAGCTGCAC TTCATTACCA TCCAGGGCGA TATGGAGACT GTGCAAGTGA TTTCTTCCAT CACTGATTAT ATCGACCAGT CAAAGTTCCC TGAGATCCAC TCTAAGCTGA AGCGCGGTGA TATTATCGGC ATTGCAGGTA AGCCGTCTCT ATCTAAGAGC AGCGAGTTTT CCCTCAAGGC AACAGAGATC ACGCTTCTTT CCACTTGCTA CCACATGCTC CCCGATGACT ATTTCGGACT TTCTTCCTTT GAGCAGCGCT TCCGTCAGCG ATACCTCGAT TTTATTGTCA ATCGCGACAA TATCAAGACC TTCATTCAGC GTGCAAATAT CATCAAGTAC ATTCGCAAAT TTTTTGATGA GCGGGACTTC GTGGAGGTCG AGACACCAGT GCTCAACCAA ATCGCCGGTG GTGCAGCGGC GCGTCCTTTT GTGACGCACC ACAACGACCT CAACCAGACC ATGTTTCTTC GTATCGCTCC AGAACTGTAC CTGAAGGAGC TCATCGTAGG TGGGATGGAT CGCGTGTACG AAATTGGCAA GCAATTCCGT AACGAGGGCA TCGATCTGAC TCACAACCCC GAATTCACGA GCTGTGAGGC GTACTGGGCA TACATGGACT ACCATGATTG GATGGCTACC ACAGAGGATC TTCTGTACGG TCTGGCCATG GAGCTTCATG GCTCACCAAT AGTTCGCTAT GCGCCGAAGG ATTCCGAGGG CAATCCGCTG CCAGAGACTA CCTTCAACTT CAGCAAGCCG TTTAAGCGTC TGCATATTAT TCCAGAGCTG GAGAAGCGCT TGCAGGTATC CTTTGATAAC GTTGACTTCG AGTCTGATGC GGGCACTCAG TTTCTGATGG ACCTGTGTAA AAAGCATAAG GCCGACTGCC CTCCGCCCTA CACGGCCGCT CGCCTTCTTG ATGCTCTAAT CGCTGAGTTC CTCGAGCCTG AGTGCCACGA CCCTTGCTTC ATTTGCGATC ACCCTCGTGT GATGTCACCT CTCGCCAAGT GGCACCGCAA TGACCCACGC CTGACAGAGC GTTTTGAGCT ATTTGTAAAC AAGAAGGAGC TTGCCAATGC ATACACCGAG CTGAACAACC CTATTGTGCA GCGCGAGGAG TTCGTTAAGC AGGTGCGGAA CCGCGACAAA GGCGATGATG AGTCTATGGA GATCGATGAG GGCTTCGTTG CAGCATTGGA GCACGCGCTG CCGCCCACTG GTGGGTGGGG TCTTGGCATC GACCGTCTTG TGATGTTCCT CACCAGTCAG TCCAACATTA AGGAGGTGCT TCTATTCCCA GCCATGAAGC CCGAAGGAAA GAATGCGATT AGCTACCCTC CGGGCACGAT GCTCAACGGT CAGGGTGTTC CTCTGTTGTA G
Details for LetaA.00825.a.B1.GE42552
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS SPEELRKQIE AIASQIADTK KTKGVDCDEY KSLVKQMATL RSQLPQDEKK LKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLNET VTIAGRIINK RSSGSKLHFI TIQGDMETVQ VISSITDYID QSKFPEIHSK LKRGDIIGIA GKPSLSKSSE FSLKATEITL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDFI VNRDNIKTFI QRANIIKYIR KFFDERDXXE VEXXXLXXIA XXXAARPFVT HHNXXXXXXX XXXXXXXYLK ELIVGXXXRV YEXXKXXXXX GIXXXXXXXF TSCEAYWAYM DYHDWMXTTE DLLYGLAMEL HGSPIVRYAP KDSEGNPLPE TTFNFSKPFK RLHIIPELEK RLQVSFDNVD FESDAGTQFL MDLCKKHKAD CPPPYTAARL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTML
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgtcct cccccgaaga gctacgtaag caaatagagg ctattgcctc tcagattgca gataccaaaa aaacgaaggg tgttgactgt gatgagtaca agtcgctggt gaagcagatg gctacgctac ggagccagct tccgcaagat gagaagaagc tgaagaagga caagacggta gagccgtcat acttcgagtc acgactggcg atggtcaagg agatgggtct cctcggtgct gcctatccgc acaagtacca ccgtcagtac acaattccac agtatcgcag gaagtacgcg cctttgctca cagaacccga cacctcactc aacgaaacag tcacaattgc tggccgcatt atcaataaac gtagctcggg ctcaaagctg cacttcatta ccatccaggg cgatatggag actgtgcaag tgatttcttc catcactgat tatatcgacc agtcaaagtt ccctgagatc cactctaagc tgaagcgcgg tgatattatc ggcattgcag gtaagccgtc tctatctaag agcagcgagt tttccctcaa ggcaacagag atcacgcttc tttccacttg ctaccacatg ctccccgatg actatttcgg actttcttcc tttgagcagc gcttccgtca gcgatacctc gattttattg tcaatcgcga caatatcaag accttcattc agcgtgcaaa tatcatcaag tacattcgca aattttttga tgagcgggac nnnnnggagg tcgagnnnnn nnnnctnnnn naaatcgccn nnnntgnagc ggcgcgtcct tttgtgacgc accacaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg tacctgaagg agctcatcgt aggtngnnnn natcgcgtgt acgaannnnn naagcnnnnn nnnaannang gcatcnannn nnnnnnnnnc cnngnnttca cgagctgtga ggcgtactgg gcatacatgg actaccatga ttggatgnnn accacagagg atcttctgta cggtctggcc atggagcttc atggctcacc aatagttcgc tatgcgccga aggattccga gggcaatccg ctgccagaga ctaccttcaa cttcagcaag ccgtttaagc gtctgcatat tattccagag ctggagaagc gcttgcaggt atcctttgat aacgttgact tcgagtctga tgcgggcact cagtttctga tggacctgtg taaaaagcat aaggccgact gccctccgcc ctacacggcc gctcgccttc ttgatgctct aatcgctgag ttcctcgagc ctgagtgcca cgacccttgc ttcatttgcg atcaccctcg tgtgatgtca cctctcgcca agtggcaccg caatgaccca cgcctgacag agcgttttga gctatttgta aacaagaagg agcttgccaa tgcatacacc gagctgaaca accctattgt gcagcgcgag gagttcgtta agcaggtgcg gaaccgcgac aaaggcgatg atgagtctat ggagatcgat gagggcttcg ttgcagcatt ggagcacgcg ctgccgccca ctggtgggtg gggtcttggc atcgaccgtc ttgtgatgtt cctcacgagt cagtccaaca ttaaggaggt gcttctattc ccagccatga agcccgaagg aaagaatgcg attagctacc ctccgggcac gatgctca
Expected Protein Sequence
MAHHHHHHMS SPEELRKQIE AIASQIADTK KTKGVDCDEY KSLVKQMATL RSQLPQDEKK LKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLNET VTIAGRIINK RSSGSKLHFI TIQGDMETVQ VISSITDYID QSKFPEIHSK LKRGDIIGIA GKPSLSKSSE FSLKATEITL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDFI VNRDNIKTFI QRANIIKYIR KFFDERDFVE VETPVLNQIA GGAAARPFVT HHNDLNQTMF LRIAPELYLK ELIVGGMDRV YEIGKQFRNE GIDLTHNPEF TSCEAYWAYM DYHDWMATTE DLLYGLAMEL HGSPIVRYAP KDSEGNPLPE TTFNFSKPFK RLHIIPELEK RLQVSFDNVD FESDAGTQFL MDLCKKHKAD CPPPYTAARL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTMLNGQG VPLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct cccccgaaga gctacgtaag caaatagagg ctattgcctc tcagattgca gataccaaaa aaacgaaggg tgttgactgt gatgagtaca agtcgctggt gaagcagatg gctacgctac ggagccagct tccgcaagat gagaagaagc tgaagaagga caagacggta gagccgtcat acttcgagtc acgactggcg atggtcaagg agatgggtct cctcggtgct gcctatccgc acaagtacca ccgtcagtac acaattccac agtatcgcag gaagtacgcg cctttgctca cagaacccga cacctcactc aacgaaacag tcacaattgc tggccgcatt atcaataagc gtagctcggg ctcaaagctg cacttcatta ccatccaggg cgatatggag actgtgcaag tgatttcttc catcactgat tatatcgacc agtcaaagtt ccctgagatc cactctaagc tgaagcgcgg tgatattatc ggcattgcag gtaagccgtc tctatctaag agcagcgagt tttccctcaa ggcaacagag atcacgcttc tttccacttg ctaccacatg ctccccgatg actatttcgg actttcttcc tttgagcagc gcttccgtca gcgatacctc gattttattg tcaatcgcga caatatcaag accttcattc agcgtgcaaa tatcatcaag tacattcgca aattttttga tgagcgggac ttcgtggagg tcgagacacc agtgctcaac caaatcgccg gtggtgcagc ggcgcgtcct tttgtgacgc accacaacga cctcaaccag accatgtttc ttcgtatcgc tccagaactg tacctgaagg agctcatcgt aggtgggatg gatcgcgtgt acgaaattgg caagcaattc cgtaacgagg gcatcgatct gactcacaac cccgaattca cgagctgtga ggcgtactgg gcatacatgg actaccatga ttggatggct accacagagg atcttctgta cggtctggcc atggagcttc atggctcacc aatagttcgc tatgcgccga aggattccga gggcaatccg ctgccagaga ctaccttcaa cttcagcaag ccgtttaagc gtctgcatat tattccagag ctggagaagc gcttgcaggt atcctttgat aacgttgact tcgagtctga tgcgggcact cagtttctga tggacctgtg taaaaagcat aaggccgact gccctccgcc ctacacggcc gctcgccttc ttgatgctct aatcgctgag ttcctcgagc ctgagtgcca cgacccttgc ttcatttgcg atcaccctcg tgtgatgtca cctctcgcca agtggcaccg caatgaccca cgcctgacag agcgttttga gctatttgta aacaagaagg agcttgccaa tgcatacacc gagctgaaca accctattgt gcagcgcgag gagttcgtta agcaggtgcg gaaccgcgac aaaggcgatg atgagtctat ggagatcgat gagggcttcg ttgcagcatt ggagcacgcg ctgccgccca ctggtgggtg gggtcttggc atcgaccgtc ttgtgatgtt cctcaccagt cagtccaaca ttaaggaggt gcttctattc ccagccatga agcccgaagg aaagaatgcg attagctacc ctccgggcac gatgctcaac ggtcagggtg ttcctctgtt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for LetaA.00825.a.B1.PW38563
PURIFICATION DATe: 11/5/2018
CONCENTRATION: 10.17mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SPEELRKQIE AIASQIADTK KTKGVDCDEY KSLVKQMATL RSQLPQDEKK LKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLNET VTIAGRIINK RSSGSKLHFI TIQGDMETVQ VISSITDYID QSKFPEIHSK LKRGDIIGIA GKPSLSKSSE FSLKATEITL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDFI VNRDNIKTFI QRANIIKYIR KFFDERDXXE VEXXXLXXIA XXXAARPFVT HHNXXXXXXX XXXXXXXYLK ELIVGXXXRV YEXXKXXXXX GIXXXXXXXF TSCEAYWAYM DYHDWMXTTE DLLYGLAMEL HGSPIVRYAP KDSEGNPLPE TTFNFSKPFK RLHIIPELEK RLQVSFDNVD FESDAGTQFL MDLCKKHKAD CPPPYTAARL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTML
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgtcct cccccgaaga gctacgtaag caaatagagg ctattgcctc tcagattgca gataccaaaa aaacgaaggg tgttgactgt gatgagtaca agtcgctggt gaagcagatg gctacgctac ggagccagct tccgcaagat gagaagaagc tgaagaagga caagacggta gagccgtcat acttcgagtc acgactggcg atggtcaagg agatgggtct cctcggtgct gcctatccgc acaagtacca ccgtcagtac acaattccac agtatcgcag gaagtacgcg cctttgctca cagaacccga cacctcactc aacgaaacag tcacaattgc tggccgcatt atcaataaac gtagctcggg ctcaaagctg cacttcatta ccatccaggg cgatatggag actgtgcaag tgatttcttc catcactgat tatatcgacc agtcaaagtt ccctgagatc cactctaagc tgaagcgcgg tgatattatc ggcattgcag gtaagccgtc tctatctaag agcagcgagt tttccctcaa ggcaacagag atcacgcttc tttccacttg ctaccacatg ctccccgatg actatttcgg actttcttcc tttgagcagc gcttccgtca gcgatacctc gattttattg tcaatcgcga caatatcaag accttcattc agcgtgcaaa tatcatcaag tacattcgca aattttttga tgagcgggac nnnnnggagg tcgagnnnnn nnnnctnnnn naaatcgccn nnnntgnagc ggcgcgtcct tttgtgacgc accacaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg tacctgaagg agctcatcgt aggtngnnnn natcgcgtgt acgaannnnn naagcnnnnn nnnaannang gcatcnannn nnnnnnnnnc cnngnnttca cgagctgtga ggcgtactgg gcatacatgg actaccatga ttggatgnnn accacagagg atcttctgta cggtctggcc atggagcttc atggctcacc aatagttcgc tatgcgccga aggattccga gggcaatccg ctgccagaga ctaccttcaa cttcagcaag ccgtttaagc gtctgcatat tattccagag ctggagaagc gcttgcaggt atcctttgat aacgttgact tcgagtctga tgcgggcact cagtttctga tggacctgtg taaaaagcat aaggccgact gccctccgcc ctacacggcc gctcgccttc ttgatgctct aatcgctgag ttcctcgagc ctgagtgcca cgacccttgc ttcatttgcg atcaccctcg tgtgatgtca cctctcgcca agtggcaccg caatgaccca cgcctgacag agcgttttga gctatttgta aacaagaagg agcttgccaa tgcatacacc gagctgaaca accctattgt gcagcgcgag gagttcgtta agcaggtgcg gaaccgcgac aaaggcgatg atgagtctat ggagatcgat gagggcttcg ttgcagcatt ggagcacgcg ctgccgccca ctggtgggtg gggtcttggc atcgaccgtc ttgtgatgtt cctcacgagt cagtccaaca ttaaggaggt gcttctattc ccagccatga agcccgaagg aaagaatgcg attagctacc ctccgggcac gatgctca
Expressed Protein Sequence
MAHHHHHHMS SPEELRKQIE AIASQIADTK KTKGVDCDEY KSLVKQMATL RSQLPQDEKK LKKDKTVEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLL TEPDTSLNET VTIAGRIINK RSSGSKLHFI TIQGDMETVQ VISSITDYID QSKFPEIHSK LKRGDIIGIA GKPSLSKSSE FSLKATEITL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDFI VNRDNIKTFI QRANIIKYIR KFFDERDFVE VETPVLNQIA GGAAARPFVT HHNDLNQTMF LRIAPELYLK ELIVGGMDRV YEIGKQFRNE GIDLTHNPEF TSCEAYWAYM DYHDWMATTE DLLYGLAMEL HGSPIVRYAP KDSEGNPLPE TTFNFSKPFK RLHIIPELEK RLQVSFDNVD FESDAGTQFL MDLCKKHKAD CPPPYTAARL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQVRNRDKGD DESMEIDEGF VAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTMLNGQG VPLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT CCCCCGAAGA GCTACGTAAG CAAATAGAGG CTATTGCCTC TCAGATTGCA GATACCAAAA AAACGAAGGG TGTTGACTGT GATGAGTACA AGTCGCTGGT GAAGCAGATG GCTACGCTAC GGAGCCAGCT TCCGCAAGAT GAGAAGAAGC TGAAGAAGGA CAAGACGGTA GAGCCGTCAT ACTTCGAGTC ACGACTGGCG ATGGTCAAGG AGATGGGTCT CCTCGGTGCT GCCTATCCGC ACAAGTACCA CCGTCAGTAC ACAATTCCAC AGTATCGCAG GAAGTACGCG CCTTTGCTCA CAGAACCCGA CACCTCACTC AACGAAACAG TCACAATTGC TGGCCGCATT ATCAATAAGC GTAGCTCGGG CTCAAAGCTG CACTTCATTA CCATCCAGGG CGATATGGAG ACTGTGCAAG TGATTTCTTC CATCACTGAT TATATCGACC AGTCAAAGTT CCCTGAGATC CACTCTAAGC TGAAGCGCGG TGATATTATC GGCATTGCAG GTAAGCCGTC TCTATCTAAG AGCAGCGAGT TTTCCCTCAA GGCAACAGAG ATCACGCTTC TTTCCACTTG CTACCACATG CTCCCCGATG ACTATTTCGG ACTTTCTTCC TTTGAGCAGC GCTTCCGTCA GCGATACCTC GATTTTATTG TCAATCGCGA CAATATCAAG ACCTTCATTC AGCGTGCAAA TATCATCAAG TACATTCGCA AATTTTTTGA TGAGCGGGAC TTCGTGGAGG TCGAGACACC AGTGCTCAAC CAAATCGCCG GTGGTGCAGC GGCGCGTCCT TTTGTGACGC ACCACAACGA CCTCAACCAG ACCATGTTTC TTCGTATCGC TCCAGAACTG TACCTGAAGG AGCTCATCGT AGGTGGGATG GATCGCGTGT ACGAAATTGG CAAGCAATTC CGTAACGAGG GCATCGATCT GACTCACAAC CCCGAATTCA CGAGCTGTGA GGCGTACTGG GCATACATGG ACTACCATGA TTGGATGGCT ACCACAGAGG ATCTTCTGTA CGGTCTGGCC ATGGAGCTTC ATGGCTCACC AATAGTTCGC TATGCGCCGA AGGATTCCGA GGGCAATCCG CTGCCAGAGA CTACCTTCAA CTTCAGCAAG CCGTTTAAGC GTCTGCATAT TATTCCAGAG CTGGAGAAGC GCTTGCAGGT ATCCTTTGAT AACGTTGACT TCGAGTCTGA TGCGGGCACT CAGTTTCTGA TGGACCTGTG TAAAAAGCAT AAGGCCGACT GCCCTCCGCC CTACACGGCC GCTCGCCTTC TTGATGCTCT AATCGCTGAG TTCCTCGAGC CTGAGTGCCA CGACCCTTGC TTCATTTGCG ATCACCCTCG TGTGATGTCA CCTCTCGCCA AGTGGCACCG CAATGACCCA CGCCTGACAG AGCGTTTTGA GCTATTTGTA AACAAGAAGG AGCTTGCCAA TGCATACACC GAGCTGAACA ACCCTATTGT GCAGCGCGAG GAGTTCGTTA AGCAGGTGCG GAACCGCGAC AAAGGCGATG ATGAGTCTAT GGAGATCGAT GAGGGCTTCG TTGCAGCATT GGAGCACGCG CTGCCGCCCA CTGGTGGGTG GGGTCTTGGC ATCGACCGTC TTGTGATGTT CCTCACCAGT CAGTCCAACA TTAAGGAGGT GCTTCTATTC CCAGCCATGA AGCCCGAAGG AAAGAATGCG ATTAGCTACC CTCCGGGCAC GATGCTCAAC GGTCAGGGTG TTCCTCTGTT GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT