SSGCID: Target Page

LetaA.00612.a

Lysyl-tRNA synthetase, putative

CENTER ID:	LetaA.00612.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00612.a.B2.GE42551	soluble domain		44	721

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00612.a.B2.PW38573	soluble domain		44	721

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP30.0190

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRPRRLCLSS VRAGMRRVTK APAAALLLPS LNFQYRRTSA VVCQDVSMTA DTASLTAEGA ASSACASPPT SAPENHATVG AEREASRRKE RRRKGRDRSP ASTATSPARQ ASRSQRSGAA STTVSAGATL PLPRTILDFP NAYQPFDAVT PLAEFREKYD YIRADQRCSD ASGVRVGGRV VSIRDMGSIL FITIRSGEAQ LQVLRQVNES FSKADLRAFA NTLHTGDILG ATGVPGRTVK GELSLYATEA SILVPYVCTD QALCPDLKGF VPLTDTGIKY RYRFVDMMTN REVVQHFRKR HAITRALRNF LDARSFIEVE TPVLHSVASG ANAKPFVTHH NANNADLFLR VAPELYLKQC VVGGMERVYE IGRVFRNEDA DRSHNPEFTT CEFYAAYHTY EDLMRMTEEI LRHLALAANG TTKISVTSCT ANESVEIDLA QPFRRVNAYD AVQEAAGVDL PPPTELNTPR GLAYMSAIML RYNIPLPSVR TAAKMFDKLI DYFITDRVVE PTFVTDHPIF MSPLAMEQRA RPGLSARFEL FINGTEYCNA YSELNDPQEQ YYRFQQQLLD RQTGDDEAMA LDETFLKALQ VGLPPTAGWG MGIDRVAMLL NGSSNIREEI LFPLLRHDAT SHDAKRRCKS ASFFGFNHNM TLFCLNALEE EMLKRAAPTE SLVKIRQLRQ CLATMQQRDM ETHAPDTGAS RGWRYEATMA VLRLFCCRGK Y

NT Sequence

ATGCGTCCTC GTCGTCTCTG CCTGTCTTCG GTGAGGGCGG GGATGCGCCG TGTTACTAAA GCACCGGCAG CAGCGCTGCT GCTGCCCTCT TTGAACTTCC AATACCGGAG AACATCCGCC GTCGTCTGCC AGGATGTATC CATGACTGCT GACACTGCGT CACTGACGGC AGAAGGGGCT GCCTCGAGCG CCTGTGCCTC TCCTCCGACG TCGGCACCTG AGAACCACGC CACCGTCGGT GCTGAGAGGG AGGCGTCAAG ACGCAAAGAG CGGAGGCGGA AAGGGCGAGA TCGGAGCCCT GCCTCAACCG CTACATCTCC TGCCCGTCAA GCTTCGAGAA GCCAGCGCTC GGGAGCCGCA TCCACCACTG TCTCGGCTGG TGCCACTCTT CCACTGCCGC GCACCATTCT TGACTTCCCA AATGCGTATC AGCCATTCGA CGCCGTCACG CCACTCGCGG AGTTTCGCGA AAAATACGAC TACATCAGGG CGGATCAGCG GTGCAGCGAC GCATCTGGAG TACGCGTTGG GGGCCGTGTC GTGAGCATCC GGGATATGGG AAGTATTCTC TTCATCACCA TCCGTAGTGG CGAGGCCCAA CTGCAGGTGC TACGGCAGGT GAACGAGTCC TTCTCCAAGG CCGACCTGCG TGCCTTTGCC AACACACTGC ACACGGGCGA CATTCTCGGT GCCACCGGCG TGCCAGGCCG CACCGTGAAA GGGGAACTCT CCCTCTACGC AACTGAGGCC TCCATCTTGG TACCGTACGT CTGCACAGAC CAGGCGCTCT GCCCCGACCT GAAAGGCTTC GTACCGCTCA CGGACACCGG CATTAAGTAC CGCTACCGCT TTGTCGACAT GATGACCAAC CGGGAGGTGG TACAGCACTT TCGAAAGCGC CACGCCATCA CTCGAGCTCT GCGCAACTTT CTCGACGCGC GCAGCTTTAT CGAAGTGGAG ACGCCGGTGC TGCACTCTGT CGCCTCAGGC GCCAACGCAA AACCGTTTGT CACGCATCAC AACGCCAACA ACGCGGATCT CTTCCTTCGG GTGGCCCCGG AGCTTTACCT AAAGCAGTGC GTTGTGGGTG GCATGGAGCG CGTGTACGAG ATCGGCCGCG TGTTCCGGAA CGAGGACGCT GACCGTAGCC ACAACCCCGA ATTCACGACG TGCGAATTCT ACGCCGCCTA TCACACCTAC GAGGATCTGA TGAGAATGAC GGAGGAGATC CTGCGTCACT TGGCGCTCGC AGCGAACGGC ACCACCAAAA TATCTGTGAC GTCATGTACG GCAAACGAGT CCGTGGAAAT TGACCTTGCG CAGCCCTTTC GCCGCGTCAA CGCGTACGAT GCAGTGCAGG AGGCGGCCGG TGTCGATCTC CCACCCCCGA CAGAGCTGAA TACGCCGCGT GGATTGGCGT ACATGTCTGC CATCATGCTC CGCTACAACA TCCCACTTCC GTCGGTGCGC ACGGCTGCTA AGATGTTCGA CAAGCTCATC GACTACTTCA TCACTGATCG CGTGGTGGAA CCGACGTTCG TGACGGACCA TCCGATCTTC ATGAGCCCGC TGGCAATGGA GCAGCGCGCG CGACCGGGGC TGTCGGCTCG CTTCGAGTTG TTCATCAATG GCACCGAATA CTGCAACGCG TACAGCGAAT TGAATGATCC ACAGGAGCAG TATTACCGCT TCCAGCAGCA ACTGCTGGAC AGGCAAACCG GTGACGACGA GGCAATGGCG CTGGACGAAA CATTTCTCAA GGCGCTGCAG GTTGGCCTAC CCCCAACGGC GGGGTGGGGG ATGGGGATCG ATCGTGTTGC CATGCTCCTC AACGGGTCCT CGAACATCCG AGAGGAGATT TTGTTCCCCC TGCTTCGCCA CGACGCCACG TCGCACGATG CGAAGCGCCG GTGCAAGTCG GCTTCATTTT TCGGCTTCAA CCACAACATG ACGCTCTTCT GTCTCAACGC GCTCGAGGAG GAGATGCTGA AGCGCGCCGC ACCGACGGAG TCTCTCGTGA AAATACGTCA GCTGCGGCAG TGCCTGGCCA CGATGCAGCA GCGCGACATG GAGACGCACG CACCTGATAC GGGCGCATCG AGAGGATGGC GCTATGAGGC CACCATGGCT GTACTTCGCC TTTTCTGCTG CCGCGGAAAG TACTGA

Details for LetaA.00612.a.B2.GE42551

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	77kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHQD VSMTADTASL TAEGAASSAC ASPPTSAPEN HATVGAEREA SRRKERRRKG RDRSPASTAT SPARQASRSQ RSGAASTTVS AGATLPLPRT ILDFPNAYQP FDAVTPLAEF REKYDYIRAD QRCSDASGVR VGGRVVSIRD MGSILFITIR SGEAQLQVLR QVNESFSKAD LRAFANTLHT GDILGATGVP GRTVKGELSL YATEASILVP YVCTDQALCP DLKGFVPLTD TGIKYRYRFV DMMTNREVVQ HFRKRHAITR ALRNFLDARS FIEVETPVLH SVASGANAKP FVTHHNANNA DLFLRVAPEL YLKQCVVGGM ERVYEIGRVF RNEDADRSHN PEFTTCEFYA AYHTYEDLMR MTEEILRHLA LAANGTTKIS VTSCTANESV EIDLAQPFRR VNAYDAVQEA AGVDLPPPTE LNTPRGLAYM SAIMLRYNIP LPSVRTAAKM FDKLIDYFIT DRVVEPTFVT DHPIFMSPLA MEQRARPGLS ARFELFINGT EYCNAYSELN DPQEQYYRFQ QQLLDRQTGD DEAMALDETF LKALQVGLPP TAGWGMGIDR VAMLLNGSSN IREEILFPLL RHDATSHDAK RRCKSASFFG FNHNMTLFCL NALEEEMLKR AAPTESLVKI RQLRQCLATM QQRDMETHAP DTGASRGWRY EATMAVLRLF CCRGKY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggatg tatccatgac tgctgacact gcgtcactga cggcagaagg ggctgcctcg agcgcctgtg cctctcctcc gacgtcggca cctgagaacc acgccaccgt cggtgctgag agggaggcgt caagacgcaa agagcggagg cggaaagggc gagatcggag ccctgcctca accgctacat ctcctgcccg tcaagcttcg agaagccagc gctcgggagc cgcatccacc actgtctcgg ctggtgccac tcttccactg ccgcgcacca ttcttgactt cccaaatgcg tatcagccat tcgacgccgt cacgccactc gcggagtttc gcgaaaaata cgactacatc agggcggatc agcggtgcag cgacgcatct ggagtacgcg ttgggggccg tgtcgtgagc atccgggata tgggaagtat tctcttcatc accatccgta gtggcgaggc ccaactgcag gtgctacggc aggtgaacga gtccttctcc aaggccgacc tgcgtgcctt tgccaacaca ctgcacacgg gcgacattct cggtgccacc ggcgtgccag gccgcaccgt gaaaggggaa ctctccctct acgcaactga ggcctccatc ttggtaccgt acgtctgcac agaccaggcg ctctgccccg acctgaaagg cttcgtaccg ctcacggaca ccggcattaa gtaccgctac cgctttgtcg acatgatgac caaccgggag gtggtacagc actttcgaaa gcgccacgcc atcactcgag ctctgcgcaa ctttctcgac gcgcgcagct ttatcgaagt ggagacgccg gtgctgcact ctgtcgcctc aggcgccaac gcaaaaccgt ttgtcacgca tcacaacgcc aacaacgcgg atctcttcct tcgggtggcc ccggagcttt acctaaagca gtgcgttgtg ggtggcatgg agcgcgtgta cgagatcggc cgcgtgttcc ggaacgagga cgctgaccgt agccacaacc ccgaattcac gacgtgcgaa ttctacgccg cctatcacac ctacgaggat ctgatgagaa tgacggagga gatcctgcgt cacttggcgc tcgcagcgaa cggcaccacc aaaatatctg tgacgtcatg tacggcaaac gagtccgtgg aaattgacct tgcgcagccc tttcgccgcg tcaacgcgta cgatgcagtg caggaggcgg ccggtgtcga tctcccaccc ccgacagagc tgaatacgcc gcgtggattg gcgtacatgt ctgccatcat gctccgctac aacatcccac ttccgtcggt gcgcacggct gctaagatgt tcgacaagct catcgactac ttcatcactg atcgcgtggt ggaaccgacg ttcgtgacgg accatccgat cttcatgagc ccgctggcaa tggagcagcg cgcgcgaccg gggctgtcgg ctcgcttcga gttgttcatc aatggcaccg aatactgcaa cgcgtacagc gaattgaatg atccacagga gcagtattac cgcttccagc agcaactgct ggacaggcaa accggtgacg acgaggcaat ggcgctggac gaaacatttc tcaaggcgct gcaggttggc ctacccccaa cggcggggtg ggggatgggg atcgatcgtg ttgccatgct cctcaacggg tcctcgaaca tccgagagga gattttgttc cccctgcttc gccacgacgc cacgtcgcac gatgcgaagc gccggtgcaa gtcggcttca tttttcggct tcaaccacaa catgacgctc ttctgtctca acgcgctcga ggaggagatg ctgaagcgcg ccgcaccgac ggagtctctc gtgaaaatac gtcagctgcg gcagtgcctg gccacgatgc agcagcgcga catggagacg cacgcacctg atacgggcgc atcgagagga tggcgctatg aggccaccat ggctgtactt cgccttttct gctgccgcgg aaagtactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for LetaA.00612.a.B2.PW38573

PURIFICATION DATe:	12/3/2018
CONCENTRATION:	2.5mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP, 1mM L-Lysine
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	22
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAGGATG TATCCATGAC TGCTGACACT GCGTCACTGA CGGCAGAAGG GGCTGCCTCG AGCGCCTGTG CCTCTCCTCC GACGTCGGCA CCTGAGAACC ACGCCACCGT CGGTGCTGAG AGGGAGGCGT CAAGACGCAA AGAGCGGAGG CGGAAAGGGC GAGATCGGAG CCCTGCCTCA ACCGCTACAT CTCCTGCCCG TCAAGCTTCG AGAAGCCAGC GCTCGGGAGC CGCATCCACC ACTGTCTCGG CTGGTGCCAC TCTTCCACTG CCGCGCACCA TTCTTGACTT CCCAAATGCG TATCAGCCAT TCGACGCCGT CACGCCACTC GCGGAGTTTC GCGAAAAATA CGACTACATC AGGGCGGATC AGCGGTGCAG CGACGCATCT GGAGTACGCG TTGGGGGCCG TGTCGTGAGC ATCCGGGATA TGGGAAGTAT TCTCTTCATC ACCATCCGTA GTGGCGAGGC CCAACTGCAG GTGCTACGGC AGGTGAACGA GTCCTTCTCC AAGGCCGACC TGCGTGCCTT TGCCAACACA CTGCACACGG GCGACATTCT CGGTGCCACC GGCGTGCCAG GCCGCACCGT GAAAGGGGAA CTCTCCCTCT ACGCAACTGA GGCCTCCATC TTGGTACCGT ACGTCTGCAC AGACCAGGCG CTCTGCCCCG ACCTGAAAGG CTTCGTACCG CTCACGGACA CCGGCATTAA GTACCGCTAC CGCTTTGTCG ACATGATGAC CAACCGGGAG GTGGTACAGC ACTTTCGAAA GCGCCACGCC ATCACTCGAG CTCTGCGCAA CTTTCTCGAC GCGCGCAGCT TTATCGAAGT GGAGACGCCG GTGCTGCACT CTGTCGCCTC AGGCGCCAAC GCAAAACCGT TTGTCACGCA TCACAACGCC AACAACGCGG ATCTCTTCCT TCGGGTGGCC CCGGAGCTTT ACCTAAAGCA GTGCGTTGTG GGTGGCATGG AGCGCGTGTA CGAGATCGGC CGCGTGTTCC GGAACGAGGA CGCTGACCGT AGCCACAACC CCGAATTCAC GACGTGCGAA TTCTACGCCG CCTATCACAC CTACGAGGAT CTGATGAGAA TGACGGAGGA GATCCTGCGT CACTTGGCGC TCGCAGCGAA CGGCACCACC AAAATATCTG TGACGTCATG TACGGCAAAC GAGTCCGTGG AAATTGACCT TGCGCAGCCC TTTCGCCGCG TCAACGCGTA CGATGCAGTG CAGGAGGCGG CCGGTGTCGA TCTCCCACCC CCGACAGAGC TGAATACGCC GCGTGGATTG GCGTACATGT CTGCCATCAT GCTCCGCTAC AACATCCCAC TTCCGTCGGT GCGCACGGCT GCTAAGATGT TCGACAAGCT CATCGACTAC TTCATCACTG ATCGCGTGGT GGAACCGACG TTCGTGACGG ACCATCCGAT CTTCATGAGC CCGCTGGCAA TGGAGCAGCG CGCGCGACCG GGGCTGTCGG CTCGCTTCGA GTTGTTCATC AATGGCACCG AATACTGCAA CGCGTACAGC GAATTGAATG ATCCACAGGA GCAGTATTAC CGCTTCCAGC AGCAACTGCT GGACAGGCAA ACCGGTGACG ACGAGGCAAT GGCGCTGGAC GAAACATTTC TCAAGGCGCT GCAGGTTGGC CTACCCCCAA CGGCGGGGTG GGGGATGGGG ATCGATCGTG TTGCCATGCT CCTCAACGGG TCCTCGAACA TCCGAGAGGA GATTTTGTTC CCCCTGCTTC GCCACGACGC CACGTCGCAC GATGCGAAGC GCCGGTGCAA GTCGGCTTCA TTTTTCGGCT TCAACCACAA CATGACGCTC TTCTGTCTCA ACGCGCTCGA GGAGGAGATG CTGAAGCGCG CCGCACCGAC GGAGTCTCTC GTGAAAATAC GTCAGCTGCG GCAGTGCCTG GCCACGATGC AGCAGCGCGA CATGGAGACG CACGCACCTG ATACGGGCGC ATCGAGAGGA TGGCGCTATG AGGCCACCAT GGCTGTACTT CGCCTTTTCT GCTGCCGCGG AAAGTACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT