SSGCID: Target Page

LetaA.00600.b

Tryptophanyl-tRNA synthetase, putative

CENTER ID:	LetaA.00600.b
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00600.b.B2.GE42550	soluble domain		25	475

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00600.b.B2.PW38550	soluble domain		25	475

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP23.0360

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRFTCRPWA LHTGLHAAAA RCNAAAYSGR TSESAEHPRE SDTGAEDIIT PWVVTAKGPK GVNYDRVLTT FRAERVDDSA RQHMRDVITK CHQRAVKAPS ATPEDVSTAS STATSPAMQQ PPSPPSPLRL HHFFKRDIAF SHRDLHKALV DIEASINTGE HSVFLYTGRG PSAGSMHLGH VLPFLLTKYL QDVFDLPLVI QITDDEKFLF RDVPFEGAEA EELISNNIKD IIAFDFNPRH TFIFRNTHYM GDMYATVLRL QRSMTSNAVK HTLGITDSDN VGKLAFPATQ AAPCFSTAFC RVLKNGNRKM RCLIPCAIDQ DPFFVLTRAA ALRLKQPPPA LLHTKFLPAL KGLEHKMSSS AEENGVITLH DTDKQVRKKL RRAFSGGCAT LEEMKETGAN LEVDVAYQYL RFFCPDDTVF ADVTERYRSG TLNSGQVKEL AADCIIREVL HGWRERRAKV TDEDVAEFCS IRSIL

NT Sequence

ATGCGTCGCT TCACTTGCCG GCCATGGGCT TTGCACACCG GCTTGCATGC GGCCGCAGCA CGCTGTAACG CAGCCGCGTA CTCGGGACGC ACGAGCGAGT CTGCGGAGCA CCCGAGAGAA AGCGATACCG GTGCCGAAGA CATCATTACC CCGTGGGTGG TGACCGCCAA GGGTCCGAAA GGTGTCAACT ACGATCGTGT GCTCACCACA TTTAGGGCGG AGCGCGTGGA TGACAGTGCG CGTCAGCACA TGCGCGATGT CATAACGAAG TGCCACCAGC GGGCCGTCAA GGCCCCGTCC GCTACACCGG AGGACGTGTC CACCGCTTCT TCGACTGCCA CATCCCCTGC CATGCAGCAG CCACCATCGC CGCCATCGCC ACTGCGACTT CACCACTTCT TCAAGCGCGA CATTGCTTTC TCGCACCGCG ATTTACACAA GGCACTGGTG GATATAGAGG CTTCCATCAA CACGGGAGAG CACTCCGTTT TCCTCTACAC TGGTCGTGGG CCCAGCGCCG GTTCCATGCA CCTGGGCCAC GTGCTGCCCT TTCTGCTCAC CAAGTATCTT CAAGACGTCT TCGACCTTCC GCTCGTGATA CAGATCACCG ATGACGAGAA ATTTCTCTTT CGTGATGTTC CCTTCGAGGG CGCCGAGGCG GAGGAGCTGA TTTCCAACAA CATCAAGGAT ATCATCGCTT TCGACTTCAA CCCGCGTCAC ACCTTTATAT TCCGCAACAC GCACTACATG GGCGACATGT ACGCGACAGT GCTCCGCCTG CAGCGTAGCA TGACAAGCAA CGCAGTGAAG CACACGCTCG GCATCACGGA CAGCGATAAC GTCGGTAAGC TCGCCTTTCC TGCCACCCAA GCGGCACCCT GTTTCAGCAC CGCTTTCTGT CGTGTTCTGA AAAACGGCAA CAGGAAGATG CGGTGCCTGA TTCCATGCGC CATTGATCAG GATCCCTTCT TCGTTCTCAC GCGCGCCGCC GCGCTGCGGC TGAAGCAGCC GCCACCGGCC CTTCTTCACA CCAAATTCCT GCCGGCGCTC AAAGGCCTTG AGCACAAGAT GAGCAGCAGC GCAGAAGAGA ATGGTGTCAT TACTTTGCAT GACACGGATA AGCAGGTGCG CAAGAAACTA CGCCGCGCAT TCTCTGGGGG CTGCGCCACC CTGGAGGAGA TGAAAGAAAC GGGCGCGAAC CTGGAAGTGG ATGTGGCTTA TCAGTACCTG CGGTTCTTCT GCCCGGATGA CACCGTTTTC GCTGACGTGA CGGAGCGCTA CCGTAGCGGC ACCCTGAATA GTGGACAAGT GAAGGAACTG GCGGCAGATT GCATCATCCG TGAGGTGTTG CACGGTTGGC GGGAGCGGCG TGCAAAGGTG ACGGACGAGG ACGTAGCAGA GTTCTGCAGT ATCCGAAGCA TCCTTTGA

Details for LetaA.00600.b.B2.GE42550

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA AYSGRTSESA EHPRESDTGA EDIITPWVVT AKGPKGVNYD RVLTTFRAER VDDSARQHMR DVITKCHQRA VKAPSATPED VSTASSTATS PAMQQPPSPP SPLRLHHFFK RDIAFSHRDL HKALVDIEAS INTGEHSVFL YTGRGPSAGS MHLGHVLPFL LTKYLQDVFD LPLVIQITDD EKFLFRDVPF EGAEAEELIS NNIKDIIAFD FNPRHTFIFR NTHYMGDMYA TVLRLQRSMT SNAVKHTLGI TDSDNVGKLA FPATQAAPCF STAFCRVLKN GNRKMRCLIP CAIDQDPFFV LTRAAALRLK QPPPALLHTK FLPALKGLEH KMSSSAEENG VITLHDTDKQ VRKKLRRAFS GGCATLEEMK ETGANLEVDV AYQYLRFFCP DDTVFADVTE RYRSGTLNSG QVKELAADCI IREVLHGWRE RRAKVTDEDV AE

Validated NT Sequence

ccatggctca ccaccaccac caccatatgg ccgcgtactc gggacgcacg agcgagtctg cggagcaccc gagagaaagc gataccggtg ccgaagacat cattaccccg tgggtggtga ccgccaaggg tccgaaaggt gtcaactacg atcgtgtgct caccacattt agggcggagc gcgtggatga cagtgcgcgt cagcacatgc gcgatgtcat aacgaagtgc caccagcggg ccgtcaaggc cccgtccgct acaccggagg acgtgtccac cgcttcttcg actgccacat cccctgccat gcagcagcca ccatcgccgc catcgccact gcgacttcac cacttcttca agcgcgacat tgctttctcg caccgcgatt tacacaaggc actggtggat atagaggctt ccatcaacac gggagagcac tccgttttcc tctacactgg tcgtgggccc agcgccggtt ccatgcacct gggccacgtg ctgccctttc tgctcaccaa gtatcttcaa gacgtcttcg accttccgct cgtgatacag atcaccgatg acgagaaatt tctctttcgt gatgttccct tcgagggcgc cgaggcggag gagctgattt ccaacaacat caaggatatc atcgctttcg acttcaaccc gcgtcacacc tttatattcc gcaacacgca ctacatgggc gacatgtacg cgacagtgct ccgcctgcag cgtagcatga caagcaacgc agtgaagcac acgctcggca tcacggacag cgataacgtc ggtaagctcg cctttcctgc cacccaagcg gcaccctgtt tcagcaccgc tttctgtcgt gttctgaaaa acggcaacag gaagatgcgg tgcctgattc catgcgccat tgatcaggat cccttcttcg ttctcacgcg cgccgccgcg ctgcggctga agcagccgcc accggccctt cttcacacca aattcctgcc ggcgctcaaa ggccttgagc acaagatgag cagcagcgca gaagagaatg gtgtcattac tttgcatgac acggataagc aggtgcgcaa gaaactacgc cgcgcattct ctgggggctg cgccaccctg gaggagatga aagaaacggg cgcgaacctg gaagtggatg tggcttatca gtacctgcgg ttcttctgcc cggatgacac cgttttcgct gacgtgacgg agcgctaccg tagcggcacc ctgaatagtg gacaagtgaa ggaactggcg gcagattgca tcatccgtga ggtgttgcac ggttggcggg agcggcgtgc aaaggtgacg gacgaggacg tagcagagtt

Expected Protein Sequence

MAHHHHHHAA YSGRTSESAE HPRESDTGAE DIITPWVVTA KGPKGVNYDR VLTTFRAERV DDSARQHMRD VITKCHQRAV KAPSATPEDV STASSTATSP AMQQPPSPPS PLRLHHFFKR DIAFSHRDLH KALVDIEASI NTGEHSVFLY TGRGPSAGSM HLGHVLPFLL TKYLQDVFDL PLVIQITDDE KFLFRDVPFE GAEAEELISN NIKDIIAFDF NPRHTFIFRN THYMGDMYAT VLRLQRSMTS NAVKHTLGIT DSDNVGKLAF PATQAAPCFS TAFCRVLKNG NRKMRCLIPC AIDQDPFFVL TRAAALRLKQ PPPALLHTKF LPALKGLEHK MSSSAEENGV ITLHDTDKQV RKKLRRAFSG GCATLEEMKE TGANLEVDVA YQYLRFFCPD DTVFADVTER YRSGTLNSGQ VKELAADCII REVLHGWRER RAKVTDEDVA EFCSIRSIL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgcgt actcgggacg cacgagcgag tctgcggagc acccgagaga aagcgatacc ggtgccgaag acatcattac cccgtgggtg gtgaccgcca agggtccgaa aggtgtcaac tacgatcgtg tgctcaccac atttagggcg gagcgcgtgg atgacagtgc gcgtcagcac atgcgcgatg tcataacgaa gtgccaccag cgggccgtca aggccccgtc cgctacaccg gaggacgtgt ccaccgcttc ttcgactgcc acatcccctg ccatgcagca gccaccatcg ccgccatcgc cactgcgact tcaccacttc ttcaagcgcg acattgcttt ctcgcaccgc gatttacaca aggcactggt ggatatagag gcttccatca acacgggaga gcactccgtt ttcctctaca ctggtcgtgg gcccagcgcc ggttccatgc acctgggcca cgtgctgccc tttctgctca ccaagtatct tcaagacgtc ttcgaccttc cgctcgtgat acagatcacc gatgacgaga aatttctctt tcgtgatgtt cccttcgagg gcgccgaggc ggaggagctg atttccaaca acatcaagga tatcatcgct ttcgacttca acccgcgtca cacctttata ttccgcaaca cgcactacat gggcgacatg tacgcgacag tgctccgcct gcagcgtagc atgacaagca acgcagtgaa gcacacgctc ggcatcacgg acagcgataa cgtcggtaag ctcgcctttc ctgccaccca agcggcaccc tgtttcagca ccgctttctg tcgtgttctg aaaaacggca acaggaagat gcggtgcctg attccatgcg ccattgatca ggatcccttc ttcgttctca cgcgcgccgc cgcgctgcgg ctgaagcagc cgccaccggc ccttcttcac accaaattcc tgccggcgct caaaggcctt gagcacaaga tgagcagcag cgcagaagag aatggtgtca ttactttgca tgacacggat aagcaggtgc gcaagaaact acgccgcgca ttctctgggg gctgcgccac cctggaggag atgaaagaaa cgggcgcgaa cctggaagtg gatgtggctt atcagtacct gcggttcttc tgcccggatg acaccgtttt cgctgacgtg acggagcgct accgtagcgg caccctgaat agtggacaag tgaaggaact ggcggcagat tgcatcatcc gtgaggtgtt gcacggttgg cgggagcggc gtgcaaaggt gacggacgag gacgtagcag agttctgcag tatccgaagc atcctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for LetaA.00600.b.B2.PW38550

PURIFICATION DATe:	10/16/2018
CONCENTRATION:	26.11mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	220µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGCGT ACTCGGGACG CACGAGCGAG TCTGCGGAGC ACCCGAGAGA AAGCGATACC GGTGCCGAAG ACATCATTAC CCCGTGGGTG GTGACCGCCA AGGGTCCGAA AGGTGTCAAC TACGATCGTG TGCTCACCAC ATTTAGGGCG GAGCGCGTGG ATGACAGTGC GCGTCAGCAC ATGCGCGATG TCATAACGAA GTGCCACCAG CGGGCCGTCA AGGCCCCGTC CGCTACACCG GAGGACGTGT CCACCGCTTC TTCGACTGCC ACATCCCCTG CCATGCAGCA GCCACCATCG CCGCCATCGC CACTGCGACT TCACCACTTC TTCAAGCGCG ACATTGCTTT CTCGCACCGC GATTTACACA AGGCACTGGT GGATATAGAG GCTTCCATCA ACACGGGAGA GCACTCCGTT TTCCTCTACA CTGGTCGTGG GCCCAGCGCC GGTTCCATGC ACCTGGGCCA CGTGCTGCCC TTTCTGCTCA CCAAGTATCT TCAAGACGTC TTCGACCTTC CGCTCGTGAT ACAGATCACC GATGACGAGA AATTTCTCTT TCGTGATGTT CCCTTCGAGG GCGCCGAGGC GGAGGAGCTG ATTTCCAACA ACATCAAGGA TATCATCGCT TTCGACTTCA ACCCGCGTCA CACCTTTATA TTCCGCAACA CGCACTACAT GGGCGACATG TACGCGACAG TGCTCCGCCT GCAGCGTAGC ATGACAAGCA ACGCAGTGAA GCACACGCTC GGCATCACGG ACAGCGATAA CGTCGGTAAG CTCGCCTTTC CTGCCACCCA AGCGGCACCC TGTTTCAGCA CCGCTTTCTG TCGTGTTCTG AAAAACGGCA ACAGGAAGAT GCGGTGCCTG ATTCCATGCG CCATTGATCA GGATCCCTTC TTCGTTCTCA CGCGCGCCGC CGCGCTGCGG CTGAAGCAGC CGCCACCGGC CCTTCTTCAC ACCAAATTCC TGCCGGCGCT CAAAGGCCTT GAGCACAAGA TGAGCAGCAG CGCAGAAGAG AATGGTGTCA TTACTTTGCA TGACACGGAT AAGCAGGTGC GCAAGAAACT ACGCCGCGCA TTCTCTGGGG GCTGCGCCAC CCTGGAGGAG ATGAAAGAAA CGGGCGCGAA CCTGGAAGTG GATGTGGCTT ATCAGTACCT GCGGTTCTTC TGCCCGGATG ACACCGTTTT CGCTGACGTG ACGGAGCGCT ACCGTAGCGG CACCCTGAAT AGTGGACAAG TGAAGGAACT GGCGGCAGAT TGCATCATCC GTGAGGTGTT GCACGGTTGG CGGGAGCGGC GTGCAAAGGT GACGGACGAG GACGTAGCAG AGTTCTGCAG TATCCGAAGC ATCCTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T