SSGCID: Target Page

LetaA.00600.a

Tryptophanyl-tRNA synthetase, putative

CENTER ID:	LetaA.00600.a
ORGANISM:	Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00600.a.B2.GE42549	soluble domain		71	467

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LetaA.00600.a.B2.PW38564	soluble domain		71	467

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LtaP29.0060

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLSFSGASI LHASITFYRC CLPSLMKTRV TLLHYLLRQD RLRLHNRIEF LAALFFLVSL CFPSFVAHII RMEDPAAQDA VVTPWTVEGD VNYDKLIKHF GCQAIDEKLL ERVERLTGKK PHHFLRRGIF FSHRDLNLIL DAYEKGQPFY LYTGRGPSSE SMHVGHLIPF MFTKWLQDTF QVPLVIQLTD DEKFFFKDLT MEEIDKMTTE NIKDIIALGF DPKLTFIFRD FEYVGQMYRI VARIEKAYTA SQVRGCFGFK MEDNCGRWMF PAIQAAPSFS VAFPHIFPPE KGNVFCMIPQ AIDQDPYFRL TRDVAPRMGY LKPAVIHSKF FPGLCGSKGK MSSSTGAAVF LTDTPKMIKD KINKHAFSGG GADKKEQLLL GGNTNVDVPV QWLRFFLEDD EELRRLQRDY MLGRIMTGDV KKVLIQRLTN IVTAHQEARK KVTEADVELF TAVRLMGPAK EEAECRS

NT Sequence

ATGCCTCTTT CGTTCTCAGG CGCGTCCATC CTTCATGCTT CTATTACCTT CTATCGCTGT TGTTTGCCTT CACTCATGAA AACACGTGTT ACACTGCTTC ACTACCTGCT CAGGCAAGAT CGACTGCGGC TTCACAACCG AATCGAATTT CTCGCCGCAC TTTTTTTTTT GGTCTCGCTG TGTTTTCCGT CGTTTGTTGC CCACATCATA AGGATGGAGG ACCCGGCTGC GCAGGATGCT GTGGTAACCC CGTGGACAGT GGAGGGTGAC GTGAATTATG ACAAGCTCAT CAAACATTTT GGGTGCCAGG CGATCGACGA AAAGCTGCTG GAGCGGGTGG AGCGACTGAC CGGGAAAAAA CCTCATCACT TTCTGCGCCG CGGAATATTC TTCTCTCACC GTGATTTGAA CCTAATTCTC GATGCGTACG AGAAGGGGCA GCCGTTCTAT CTGTACACCG GCCGAGGGCC CAGCAGCGAG TCGATGCATG TTGGGCATCT CATACCTTTT ATGTTCACCA AGTGGCTGCA GGACACTTTC CAGGTGCCAC TGGTGATTCA GCTCACCGAC GACGAAAAGT TCTTCTTCAA GGATTTGACG ATGGAGGAGA TCGATAAGAT GACGACGGAG AACATAAAGG ACATCATTGC CTTGGGTTTT GACCCGAAGC TGACGTTTAT ATTCAGGGAT TTTGAGTATG TCGGACAAAT GTATCGCATT GTCGCACGCA TCGAGAAGGC GTACACCGCT AGCCAGGTGC GGGGCTGCTT CGGCTTCAAG ATGGAGGACA ACTGCGGCCG CTGGATGTTC CCTGCGATTC AGGCAGCGCC ATCCTTCTCA GTGGCGTTTC CACACATTTT TCCGCCGGAG AAAGGAAACG TCTTTTGTAT GATTCCGCAG GCCATCGACC AAGATCCGTA CTTCCGACTT ACGCGTGATG TGGCACCCCG TATGGGCTAT TTGAAGCCAG CCGTCATACA CTCCAAGTTC TTCCCGGGTC TTTGTGGGTC AAAGGGCAAG ATGAGCTCAT CCACCGGTGC GGCTGTCTTT CTGACAGATA CTCCTAAGAT GATTAAAGAC AAAATTAACA AGCACGCGTT CAGCGGTGGT GGCGCGGATA AGAAGGAACA GCTGCTTCTT GGTGGCAACA CCAATGTGGA CGTACCCGTA CAGTGGCTGC GCTTTTTCCT CGAGGATGAC GAGGAGTTGA GGCGACTCCA GAGGGACTAC ATGCTTGGTC GCATCATGAC GGGTGACGTA AAGAAAGTTC TCATTCAAAG ACTTACCAAC ATCGTCACGG CACACCAAGA AGCGCGGAAA AAGGTGACGG AAGCCGATGT GGAGCTCTTT ACCGCTGTCC GCTTGATGGG GCCGGCGAAG GAGGAGGCAG AATGCAGGAG TTAA

Details for LetaA.00600.a.B2.GE42549

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR MEDPAAQDAV VTPWTVEGDV NYDKLIKHFG CQAIDEKLLE RVERLTGKKP HHFLRRGIFF SHRDLNLILD AYEKGQPFYL YTGRGPSSES MHVGHLIPFM FTKWLQDTFQ VPLVIQLTDD EKFFFKDLTM EEIDKMTTEN IKDIIALGFD PKLTFIFRDF EYVGQMYRIV ARIEKAYTAS QVRGCFGFKM EDNCGRWMFP AIQAAPSFSV AFPHIFPPEK GNVFCMIPQA IDQDPYFRLT RDVAPRMGYL KPAVIHSKFF PGLCGSKGKM SSSTGAAVFL TDTPKMIKDK INKHAFSGGG ADKKEQLLLG GNTNVDVPVQ WLRFFLEDDE ELRRLQRDYM LGRIMTGDVK KVLIQRLTNI VTAHQEARKK VTEADVELFT AVRLMGP

Validated NT Sequence

accatggctc accaccacca ccaccatatg aggatggagg acccggctgc gcaggatgct gtggtaaccc cgtggacagt ggagggtgac gtgaattatg acaagctcat caaacatttt gggtgccagg cgatcgacga aaagctgctg gagcgggtgg agcgactgac cgggaaaaaa cctcatcact ttctgcgccg cggaatattc ttctctcacc gtgatttaaa cctaattctc gatgcgtacg agaaggggca gccgttctat ctgtacaccg gccgagggcc cagcagcgag tcgatgcatg ttgggcatct catacctttt atgttcacca agtggctgca ggacactttc caggtgccac tggtgattca gctcaccgac gacgaaaagt tcttcttcaa ggatttgacg atggaggaga tcgataagat gacgacggag aacataaagg acatcattgc cttgggtttt gacccgaagc tgacgtttat attcagggat tttgagtatg tcggacaaat gtatcgcatt gtcgcacgca tcgagaaggc gtacaccgct agccaggtgc ggggctgctt cggcttcaag atggaggaca actgcggccg ctggatgttc cctgcgattc aggcagcgcc atccttctca gtggcgtttc cacacatttt tccgccggag aaaggaaacg tcttttgtat gattccgcag gccatcgacc aagatccgta cttccgactt acgcgtgatg tggcaccccg tatgggctat ttgaagccag ccgtcataca ctccaagttc ttcccgggtc tttgtgggtc aaagggcaag atgagctcat ccaccggtgc ggctgtcttt ctgacagata ctcctaagat gattaaagac aaaattaaca agcacgcgtt cagcggtggt ggcgcggata agaaggaaca gctgcttctt ggtggcaaca ccaatgtgga cgtacccgta cagtggctgc gctttttcct cgaggatgac gaggagttga ggcgactcca gagggactac atgcttggtc gcatcatgac gggtgacgta aagaaagttc tcattcaaag acttaccaac atcgtcacgg cacaccaaga agcgcggaaa aaggtgacgg aagccgatgt ggagctcttt accgctgtcc gcttgatggg gccggcg

Expected Protein Sequence

MAHHHHHHRM EDPAAQDAVV TPWTVEGDVN YDKLIKHFGC QAIDEKLLER VERLTGKKPH HFLRRGIFFS HRDLNLILDA YEKGQPFYLY TGRGPSSESM HVGHLIPFMF TKWLQDTFQV PLVIQLTDDE KFFFKDLTME EIDKMTTENI KDIIALGFDP KLTFIFRDFE YVGQMYRIVA RIEKAYTASQ VRGCFGFKME DNCGRWMFPA IQAAPSFSVA FPHIFPPEKG NVFCMIPQAI DQDPYFRLTR DVAPRMGYLK PAVIHSKFFP GLCGSKGKMS SSTGAAVFLT DTPKMIKDKI NKHAFSGGGA DKKEQLLLGG NTNVDVPVQW LRFFLEDDEE LRRLQRDYML GRIMTGDVKK VLIQRLTNIV TAHQEARKKV TEADVELFTA VRLMGPAKEE AECRS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataggatgg aggacccggc tgcgcaggat gctgtggtaa ccccgtggac agtggagggt gacgtgaatt atgacaagct catcaaacat tttgggtgcc aggcgatcga cgaaaagctg ctggagcggg tggagcgact gaccgggaaa aaacctcatc actttctgcg ccgcggaata ttcttctctc accgtgattt gaacctaatt ctcgatgcgt acgagaaggg gcagccgttc tatctgtaca ccggccgagg gcccagcagc gagtcgatgc atgttgggca tctcatacct tttatgttca ccaagtggct gcaggacact ttccaggtgc cactggtgat tcagctcacc gacgacgaaa agttcttctt caaggatttg acgatggagg agatcgataa gatgacgacg gagaacataa aggacatcat tgccttgggt tttgacccga agctgacgtt tatattcagg gattttgagt atgtcggaca aatgtatcgc attgtcgcac gcatcgagaa ggcgtacacc gctagccagg tgcggggctg cttcggcttc aagatggagg acaactgcgg ccgctggatg ttccctgcga ttcaggcagc gccatccttc tcagtggcgt ttccacacat ttttccgccg gagaaaggaa acgtcttttg tatgattccg caggccatcg accaagatcc gtacttccga cttacgcgtg atgtggcacc ccgtatgggc tatttgaagc cagccgtcat acactccaag ttcttcccgg gtctttgtgg gtcaaagggc aagatgagct catccaccgg tgcggctgtc tttctgacag atactcctaa gatgattaaa gacaaaatta acaagcacgc gttcagcggt ggtggcgcgg ataagaagga acagctgctt cttggtggca acaccaatgt ggacgtaccc gtacagtggc tgcgcttttt cctcgaggat gacgaggagt tgaggcgact ccagagggac tacatgcttg gtcgcatcat gacgggtgac gtaaagaaag ttctcattca aagacttacc aacatcgtca cggcacacca agaagcgcgg aaaaaggtga cggaagccga tgtggagctc tttaccgctg tccgcttgat ggggccggcg aaggaggagg cagaatgcag gagttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for LetaA.00600.a.B2.PW38564

PURIFICATION DATe:	11/5/2018
CONCENTRATION:	20.77mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	210µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGGATGG AGGACCCGGC TGCGCAGGAT GCTGTGGTAA CCCCGTGGAC AGTGGAGGGT GACGTGAATT ATGACAAGCT CATCAAACAT TTTGGGTGCC AGGCGATCGA CGAAAAGCTG CTGGAGCGGG TGGAGCGACT GACCGGGAAA AAACCTCATC ACTTTCTGCG CCGCGGAATA TTCTTCTCTC ACCGTGATTT GAACCTAATT CTCGATGCGT ACGAGAAGGG GCAGCCGTTC TATCTGTACA CCGGCCGAGG GCCCAGCAGC GAGTCGATGC ATGTTGGGCA TCTCATACCT TTTATGTTCA CCAAGTGGCT GCAGGACACT TTCCAGGTGC CACTGGTGAT TCAGCTCACC GACGACGAAA AGTTCTTCTT CAAGGATTTG ACGATGGAGG AGATCGATAA GATGACGACG GAGAACATAA AGGACATCAT TGCCTTGGGT TTTGACCCGA AGCTGACGTT TATATTCAGG GATTTTGAGT ATGTCGGACA AATGTATCGC ATTGTCGCAC GCATCGAGAA GGCGTACACC GCTAGCCAGG TGCGGGGCTG CTTCGGCTTC AAGATGGAGG ACAACTGCGG CCGCTGGATG TTCCCTGCGA TTCAGGCAGC GCCATCCTTC TCAGTGGCGT TTCCACACAT TTTTCCGCCG GAGAAAGGAA ACGTCTTTTG TATGATTCCG CAGGCCATCG ACCAAGATCC GTACTTCCGA CTTACGCGTG ATGTGGCACC CCGTATGGGC TATTTGAAGC CAGCCGTCAT ACACTCCAAG TTCTTCCCGG GTCTTTGTGG GTCAAAGGGC AAGATGAGCT CATCCACCGG TGCGGCTGTC TTTCTGACAG ATACTCCTAA GATGATTAAA GACAAAATTA ACAAGCACGC GTTCAGCGGT GGTGGCGCGG ATAAGAAGGA ACAGCTGCTT CTTGGTGGCA ACACCAATGT GGACGTACCC GTACAGTGGC TGCGCTTTTT CCTCGAGGAT GACGAGGAGT TGAGGCGACT CCAGAGGGAC TACATGCTTG GTCGCATCAT GACGGGTGAC GTAAAGAAAG TTCTCATTCA AAGACTTACC AACATCGTCA CGGCACACCA AGAAGCGCGG AAAAAGGTGA CGGAAGCCGA TGTGGAGCTC TTTACCGCTG TCCGCTTGAT GGGGCCGGCG AAGGAGGAGG CAGAATGCAG GAGTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT