LetaA.00302.a

Cysteinyl-tRNA synthetase, putative

CENTER ID: LetaA.00302.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.00302.a.B1.GE42548 full length 1 782
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP12.0270

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNNCASKVV AGLNGAHPVK RERHPVWYPP LKLDGNGLKV MNSLTETLED FAPRDGRVVR WYTCGPTVYD LSHMGHARAY LTFDIVRRIM EDYFGYSVIY QMNITDIDDK IIKRARVNKL LEDFKEGELH HRDVAKLMAF TAEAVAAAES RLAERKAALL EPIAEGATSR AKAEREEKML EVQLKESQLA ETKEKIAAAK EDFDVLFSVA SGVNGDLLDE RSGHTVSDQQ IFEDHARKYE RAFFEDMQRL GIREPDIVTR VTEYVPQVVA FVQKIIDNGF AYLGKTSVFF DTEAYIRAGH DYPKLKPGGD RNTTEDEMAE GEGVLSKGIE GEKRSPNDFA LWKFSKPGEP RWPSPWGEGR PGWHIECSVM ASDILGENMD IHSGGWDLKF PHHDNECAQS EACNLHNQWV NYFLHCGHLH IKGLKMSKSL KNFITIRQAL DELGVTARTM RLLFLANPWY KPMNFSDQSL DEAKEKERVL RAFFGSVDIV LRTDNWHATQ GANRHDRELL SKWVETEASV HAALQDNFDT VTALQQLMSL VAAMNQYLLS GERPSATLVR KVGCYVTKMF RIFGVVEGSD DIGLQKQGSG DGEARFIEVV NTLVRFRDDV RNAAKEQKVV AGFLPLCDKV RDEWLVDAGV RLEDNPVGPT TWKSDEPALL RRELEERRAQ QEGDRRKKLA NQVETKRKLV EKWQLFTYPP SAYFRRQDEQ RGEAKYAAYD DVTGLPTRTA AGEEVNEKEA KKLSKEQAKY AKSYDEFISK GGVSWLHEQE AELASMVAEL ER
NT Sequence
ATGAACAACA ACTGTGCCTC CAAGGTAGTG GCCGGCCTCA ATGGCGCCCA CCCAGTGAAG AGGGAGCGCC ACCCCGTGTG GTACCCGCCC CTGAAGCTGG ATGGCAACGG ACTCAAGGTG ATGAACTCAT TGACAGAGAC CCTCGAGGAC TTCGCGCCGC GTGATGGCCG GGTTGTGCGG TGGTACACGT GCGGTCCGAC CGTCTACGAT CTGTCTCACA TGGGCCACGC CCGCGCTTAC CTCACCTTCG ACATCGTTCG CCGCATCATG GAGGACTACT TTGGCTACTC TGTGATTTAC CAGATGAACA TCACAGATAT CGACGACAAA ATCATCAAGC GCGCTCGCGT AAACAAACTT TTGGAAGACT TCAAGGAGGG AGAGCTGCAT CACAGAGACG TTGCCAAGCT CATGGCGTTC ACGGCAGAGG CCGTCGCCGC CGCCGAAAGC CGCCTGGCAG AGCGCAAGGC AGCGCTGTTG GAGCCGATTG CCGAAGGCGC CACCAGCCGC GCCAAGGCGG AGCGCGAGGA GAAGATGTTG GAGGTGCAGC TGAAGGAGTC GCAACTTGCC GAGACCAAGG AAAAAATTGC GGCAGCCAAG GAGGATTTTG ACGTCCTGTT CTCCGTGGCC AGCGGCGTGA ACGGTGACCT GCTTGACGAG CGCAGCGGCC ACACCGTCTC CGATCAGCAG ATCTTCGAAG ATCACGCCCG AAAGTACGAG CGTGCTTTCT TCGAGGATAT GCAGCGCTTG GGCATTCGTG AACCGGACAT CGTTACACGC GTCACCGAGT ACGTGCCGCA GGTGGTGGCA TTTGTACAGA AGATCATCGA CAACGGCTTT GCCTACCTTG GCAAGACGTC TGTCTTCTTC GACACGGAAG CGTACATCAG GGCCGGCCAC GACTACCCGA AGCTGAAGCC GGGCGGCGAT CGCAACACCA CCGAGGATGA AATGGCGGAG GGCGAGGGCG TACTGTCGAA GGGCATCGAG GGCGAGAAGC GCAGCCCGAA CGACTTTGCG CTGTGGAAGT TCTCGAAGCC CGGTGAGCCG CGCTGGCCAT CCCCGTGGGG GGAGGGCCGT CCTGGCTGGC ACATCGAGTG CTCCGTGATG GCGTCCGATA TTCTTGGCGA GAACATGGAC ATCCACAGCG GTGGGTGGGA CTTGAAGTTT CCGCACCACG ACAACGAGTG CGCGCAGAGC GAGGCCTGCA ACCTTCACAA CCAGTGGGTA AACTACTTCC TCCACTGCGG CCATCTCCAC ATCAAGGGAC TGAAGATGAG CAAGAGCCTC AAGAACTTTA TCACCATCCG CCAGGCGCTC GACGAACTCG GCGTCACCGC GCGCACGATG CGGCTTCTGT TCCTCGCTAA CCCCTGGTAT AAGCCGATGA ACTTCTCAGA CCAGTCGCTC GACGAGGCGA AGGAGAAGGA GCGTGTGCTG CGTGCCTTCT TCGGCAGCGT AGACATCGTC CTCCGCACCG ACAACTGGCA TGCCACACAG GGCGCCAACA GACACGACCG CGAGCTGTTG TCCAAGTGGG TCGAGACCGA GGCGTCCGTG CACGCGGCGC TGCAGGACAA CTTTGACACG GTCACTGCTC TGCAGCAACT CATGTCTCTC GTCGCCGCGA TGAACCAGTA CCTGCTGTCG GGCGAGCGAC CGAGTGCGAC CCTGGTGCGC AAGGTGGGCT GCTATGTGAC GAAGATGTTC CGCATCTTTG GCGTCGTCGA GGGGTCTGAC GACATTGGCC TGCAGAAGCA GGGCTCAGGT GACGGCGAGG CGCGCTTCAT CGAGGTTGTG AACACCCTCG TGCGCTTCCG TGATGACGTG CGCAATGCAG CGAAAGAGCA GAAGGTGGTG GCGGGCTTCC TGCCCCTGTG CGACAAGGTG CGCGACGAAT GGCTTGTGGA CGCTGGCGTA CGGCTCGAGG ACAACCCAGT GGGCCCGACA ACGTGGAAAA GCGACGAGCC AGCACTGCTG CGCAGGGAAC TGGAAGAGCG CCGCGCTCAG CAGGAAGGGG ATCGCCGGAA GAAGCTGGCG AACCAGGTGG AGACAAAGCG CAAGCTCGTG GAGAAGTGGC AACTGTTTAC CTACCCGCCG TCCGCCTACT TCCGTCGCCA GGACGAGCAG CGGGGGGAAG CGAAGTACGC CGCCTATGAC GATGTCACCG GTCTCCCAAC ACGGACGGCT GCCGGCGAAG AGGTGAACGA AAAGGAGGCA AAGAAGCTGA GCAAGGAGCA GGCAAAGTAC GCCAAGTCGT ACGATGAGTT CATCAGCAAG GGCGGTGTGT CGTGGCTTCA TGAGCAGGAG GCGGAGCTCG CCAGCATGGT GGCTGAGCTC GAAAGGTAA
Details for LetaA.00302.a.B1.GE42548
HARVESTED ON: data unavailable
SEQUENCED ON: 7/10/2018
EXPECTED MW: 90kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN NNCASKVVAG LNGAHPVKRE RHPVWYPPLK LDGNGLKVMN SLTETLEDFA PRDGRVVRWY TCGPTVYDLS HMGHARAYLT FDIVRRIMED YFGYSVIYQM NITDIDDKII KRARVNKLLE DFKEGELHHR DVAKLMAFTA EAVAAAESRL AERKAALLEP IAEGATSRAK AEREEKMLEV QLKESQLAET KEKIAAAKED FDVLFSVASG VNGDLLDERS GHTVSDQQIF EDHARKYERA FFEDMQRLGI REPDIVTRVT EYVPQVVAFV QKIIDNGFAY LGKTSVFFDT EAYIRAGHDY PKLKPGGDRN TTEDEMAEGE GVLSKGIEGE KRSPNDFALW KFSKPGEPRW PSPWGEGRPG WHIECSVMAS DILGENMDIH SGGWDLKFPH HDNECAQSEA CNLHNQWVNY FLHCGHLHIK GLKMSKSLKN FITIRQALDE LGVTARTMRL LFLANPWYKP MNFSDQSLDE AKEKERVLRA FFGSVDIVLR TDNWHATQGA NRHDRELLSK WVETEASVHA ALQDNFDTVT ALQQLMSLVA AMNQYLLSGE RPSATLVRKV GCYVTKMFRI FGVVEGSDDI GLQKQGSGDG EARFIEVVNT LVRFRDDVRN AAKEQKVVAG FLPLCDKVRD EWLVDAGVRL EDNPVGPTTW KSDEPALLRR ELEERRAQQE GDRRKKLANQ VETKRKLVEK WQLFTYPPSA YFRRQDEQRG EAKYAAYDDV TGLPTRTAAG EEVNEKEAKK LSKEQAKYAK SYDEFISKGG VSWLHEQEAE LASMVAELER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca acaactgtgc ctccaaggta gtggccggcc tcaatggcgc ccacccagtg aagagggagc gccaccccgt gtggtacccg cccctgaagc tggatggcaa cggactcaag gtgatgaact cattgacaga gaccctcgag gacttcgcgc cgcgtgatgg ccgggttgtg cggtggtaca cgtgcggtcc gaccgtctac gatctgtctc acatgggcca cgcccgcgct tacctcacct tcgacatcgt tcgccgcatc atggaggact actttggcta ctctgtgatt taccagatga acatcacaga tatcgacgac aaaatcatca agcgcgctcg cgtaaacaaa cttttggaag acttcaagga gggagagctg catcacagag acgttgccaa gctcatggcg ttcacggcag aggccgtcgc cgccgccgaa agccgcctgg cagagcgcaa ggcagcgctg ttggagccga ttgccgaagg cgccaccagc cgcgccaagg cggagcgcga ggagaagatg ttggaggtgc agctgaagga gtcgcaactt gccgagacca aggaaaaaat tgcggcagcc aaggaggatt ttgacgtcct gttctccgtg gccagcggcg tgaacggtga cctgcttgac gagcgcagcg gccacaccgt ctccgatcag cagatcttcg aagatcacgc ccgaaagtac gagcgtgctt tcttcgagga tatgcagcgc ttgggcattc gtgaaccgga catcgttaca cgcgtcaccg agtacgtgcc gcaggtggtg gcatttgtac agaagatcat cgacaacggc tttgcctacc ttggcaagac gtctgtcttc ttcgacacgg aagcgtacat cagggccggc cacgactacc cgaagctgaa gccgggcggc gatcgcaaca ccaccgagga tgaaatggcg gagggcgagg gcgtactgtc gaagggcatc gagggcgaga agcgcagccc gaacgacttt gcgctgtgga agttctcgaa gcccggtgag ccgcgctggc catccccgtg gggggagggc cgtcctggct ggcacatcga gtgctccgtg atggcgtccg atattcttgg cgagaacatg gacatccaca gcggtgggtg ggacttgaag tttccgcacc acgacaacga gtgcgcgcag agcgaggcct gcaaccttca caaccagtgg gtaaactact tcctccactg cggccatctc cacatcaagg gactgaagat gagcaagagc ctcaagaact ttatcaccat ccgccaggcg ctcgacgaac tcggcgtcac cgcgcgcacg atgcggcttc tgttcctcgc taacccctgg tataagccga tgaacttctc agaccagtcg ctcgacgagg cgaaggagaa ggagcgtgtg ctgcgtgcct tcttcggcag cgtagacatc gtcctccgca ccgacaactg gcatgccaca cagggcgcca acagacacga ccgcgagctg ttgtccaagt gggtcgagac cgaggcgtcc gtgcacgcgg cgctgcagga caactttgac acggtcactg ctctgcagca actcatgtct ctcgtcgccg cgatgaacca gtacctgctg tcgggcgagc gaccgagtgc gaccctggtg cgcaaggtgg gctgctatgt gacgaagatg ttccgcatct ttggcgtcgt cgaggggtct gacgacattg gcctgcagaa gcagggctca ggtgacggcg aggcgcgctt catcgaggtt gtgaacaccc tcgtgcgctt ccgtgatgac gtgcgcaatg cagcgaaaga gcagaaggtg gtggcgggct tcctgcccct gtgcgacaag gtgcgcgacg aatggcttgt ggacgctggc gtacggctcg aggacaaccc agtgggcccg acaacgtgga aaagcgacga gccagcactg ctgcgcaggg aactggaaga gcgccgcgct cagcaggaag gggatcgccg gaagaagctg gcgaaccagg tggagacaaa gcgcaagctc gtggagaagt ggcaactgtt tacctacccg ccgtccgcct acttccgtcg ccaggacgag cagcgggggg aagcgaagta cgccgcctat gacgatgtca ccggtctccc aacacggacg gctgccggcg aagaggtgaa cgaaaaggag gcaaagaagc tgagcaagga gcaggcaaag tacgccaagt cgtacgatga gttcatcagc aagggcggtg tgtcgtggct tcatgagcag gaggcggagc tcgccagcat ggtggctgag ctcgaaaggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat