LetaA.00063.a

Histidyl-tRNA synthetase, putative

CENTER ID: LetaA.00063.a
ORGANISM: Leishmania tarentolae Parrot-TarII
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LetaA.00063.a.B1.GE42547 full length 1 473
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LtaP30.0700

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASTASPNAA LLAEIHDLKV KLAEKEALLQ PEGAISKKSK KKSQANMIEK EPVQGCRDFP PEDMRVRRYL FDVFHETARK FGFEEYDAPV LESEELYIRK AGEEITEQMF NFVTKGGHRV TLRPEMTPSL VRLQLAKGRS LLLPAKWYSI PQCWRYEAIS RGRRREHYQW NMDIVGVKSV AAEVELVCAA CAAMEALGLT AQDVGVKVNS RRLLQCALAG AGVPDSLFAP VCVIVDKMEK LPEEQIRAEL KDLGLDGQQA DDIIRTLSLK SVDDFDKDLF DKNGALSEIA EFFSQIQMYG YGDWIQFDAS VVRGLAYYTG IVFEAFDRSG EFRAICGGGR YDNLFTLYGS LQPVPCVGFG FGDCVIVELL KKKKLINSLP HKVDDVVIPF NESQRGTALK VLKQLRDKGR SVDIIMDPKK VAQTFSYADR IGADRAVLIA PDEMEKGMVR VKMLREGQRG ENDRGDLVPF EDL
NT Sequence
ATGGCCTCGA CAGCATCGCC CAATGCCGCT CTTCTCGCGG AAATCCACGA CCTCAAGGTG AAACTTGCCG AGAAGGAGGC GCTTTTGCAG CCGGAGGGGG CAATATCGAA GAAGTCCAAG AAAAAGAGTC AGGCAAACAT GATTGAGAAA GAGCCAGTGC AAGGCTGCCG CGACTTCCCT CCCGAGGACA TGCGCGTTCG CAGGTACCTC TTCGACGTCT TCCACGAAAC GGCCCGAAAA TTCGGCTTCG AGGAGTACGA TGCCCCAGTT CTAGAGTCGG AGGAGCTCTA CATCCGCAAG GCAGGAGAGG AGATTACGGA GCAAATGTTC AACTTTGTGA CAAAAGGTGG CCACCGTGTT ACACTTCGCC CGGAGATGAC GCCGTCGCTG GTGCGTCTGC AGCTCGCCAA GGGCCGCTCT CTGCTTCTGC CTGCTAAATG GTACTCCATT CCGCAGTGCT GGCGTTACGA AGCCATCTCC CGCGGCCGCC GCCGTGAGCA CTACCAGTGG AATATGGATA TCGTCGGCGT GAAGAGCGTT GCCGCAGAGG TTGAGCTTGT GTGTGCGGCG TGCGCGGCGA TGGAGGCGCT TGGCCTCACC GCGCAAGATG TCGGCGTGAA GGTTAACTCC CGCAGATTGC TGCAGTGTGC ACTCGCCGGC GCCGGCGTGC CAGATTCTCT CTTTGCTCCG GTGTGCGTGA TTGTCGACAA GATGGAGAAG CTGCCTGAGG AGCAAATCCG CGCGGAGTTG AAGGACCTGG GCTTGGATGG CCAGCAGGCG GATGACATCA TCCGGACACT TAGTCTCAAG TCTGTCGACG ACTTCGATAA GGACCTCTTT GATAAGAACG GCGCCCTCTC GGAGATTGCG GAGTTCTTCT CGCAGATACA GATGTACGGG TACGGCGACT GGATCCAGTT CGATGCGAGT GTTGTGCGTG GACTTGCCTA TTACACTGGG ATCGTCTTCG AAGCGTTTGA CCGAAGTGGT GAGTTCCGCG CGATCTGCGG AGGCGGCCGC TACGACAACC TCTTCACCCT GTACGGCAGC CTGCAGCCTG TCCCGTGCGT CGGGTTTGGC TTTGGCGACT GTGTCATCGT TGAACTTCTG AAGAAGAAGA AGTTGATCAA CTCGCTGCCA CATAAGGTGG ATGATGTGGT GATTCCGTTC AATGAGTCTC AGCGAGGCAC CGCCCTGAAG GTACTGAAGC AGCTGCGTGA CAAGGGCCGC AGTGTAGACA TCATTATGGA CCCCAAGAAG GTTGCTCAGA CCTTCAGTTA CGCCGATCGT ATCGGCGCTG ACCGTGCCGT TCTGATTGCG CCAGACGAAA TGGAGAAGGG CATGGTACGC GTCAAGATGC TGCGTGAAGG ACAAAGGGGC GAGAATGACC GCGGTGACCT TGTGCCCTTC GAGGACTTGT AG
Details for LetaA.00063.a.B1.GE42547
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA STASPNAALL AEIHDLKVKL AEKEALLQPE GAISKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRYLFD VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMEALGLTAQ DVGVKVNSRR LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LGLDGQQADD IIRTLSLKSV DDFDKDLFDK NGALSEIAEF FSQIQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSLQ PVPCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSV DIIMDPKKVA QTFSYADRIG ADRAVLIAPD EMEKGMVRVK MLREGQRGEN DRG
Validated NT Sequence
atggctcacc accaccacca ccatatggcc tcgacagcat cgcccaatgc cgctcttctc gcggaaatcc acgacctcaa ggtgaaactt gccgagaagg aggcgctttt gcagccggag ggggcaatat cgaagaagtc caagaaaaag agtcaggcaa acatgattga gaaagagcca gtgcaaggct gccgcgactt ccctcccgag gacatgcgcg ttcgcaggta cctcttcgac gtcttccacg aaacggcccg aaaattcggc ttcgaggagt acgatgcccc agttctagag tcggaggagc tctacatccg caaggcagga gaggagatta cggagcaaat gttcaacttt gtgacaaaag gtggccaccg tgttacactt cgcccggaga tgacgccgtc gctggtgcgt ctgcagctcg ccaagggccg ctctctgctt ctgcctgcta aatggtactc cattccgcag tgctggcgtt acgaagccat ctcccgcggc cgccgccgtg agcactacca gtggaatatg gatatcgtcg gcgtgaagag cgttgccgca gaggttgagc ttgtgtgtgc ggcgtgcgcg gcgatggagg cgcttggcct caccgcgcaa gatgtcggcg tgaaggttaa ctcccgcaga ttgctgcagt gtgcactcgc cggcgccggc gtgccagatt ctctctttgc tccggtgtgc gtgattgtcg acaagatgga gaagctgcct gaggagcaaa tccgcgcgga gttgaaggac ctgggcttgg atggccagca ggcggatgac atcatccgga cacttagtct caagtctgtc gacgacttcg ataaggacct ctttgataag aacggcgccc tctcggagat tgcggagttc ttctcgcaga tacagatgta cgggtacggc gactggatcc agttcgatgc gagtgttgtg cgtggacttg cctattacac tgggatcgtc ttcgaagcgt ttgaccgaag tggtgagttc cgcgcgatct gcggaggcgg ccgctacgac aacctcttca ccctgtacgg cagcctgcag cctgtcccgt gcgtcgggtt tggctttggc gactgtgtca tcgttgaact tctgaagaag aagaagttga tcaactcgct gccacataag gtggatgatg tggtgattcc gttcaatgag tctcagcgag gcaccgccct gaaggtactg aagcagctgc gtgacaaggg ccgcagtgta gacatcatta tggaccccaa gaaggttgct cagaccttca gttacgccga tcgtatcggc gctgaccgtg ccgttctgat tgcgccagac gaaatggaga agggcatggt acgcgtcaag atgctgcgtg aaggacaaag gggcgagaat gaccgcggtg
Expected Protein Sequence
MAHHHHHHMA STASPNAALL AEIHDLKVKL AEKEALLQPE GAISKKSKKK SQANMIEKEP VQGCRDFPPE DMRVRRYLFD VFHETARKFG FEEYDAPVLE SEELYIRKAG EEITEQMFNF VTKGGHRVTL RPEMTPSLVR LQLAKGRSLL LPAKWYSIPQ CWRYEAISRG RRREHYQWNM DIVGVKSVAA EVELVCAACA AMEALGLTAQ DVGVKVNSRR LLQCALAGAG VPDSLFAPVC VIVDKMEKLP EEQIRAELKD LGLDGQQADD IIRTLSLKSV DDFDKDLFDK NGALSEIAEF FSQIQMYGYG DWIQFDASVV RGLAYYTGIV FEAFDRSGEF RAICGGGRYD NLFTLYGSLQ PVPCVGFGFG DCVIVELLKK KKLINSLPHK VDDVVIPFNE SQRGTALKVL KQLRDKGRSV DIIMDPKKVA QTFSYADRIG ADRAVLIAPD EMEKGMVRVK MLREGQRGEN DRGDLVPFED L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct cgacagcatc gcccaatgcc gctcttctcg cggaaatcca cgacctcaag gtgaaacttg ccgagaagga ggcgcttttg cagccggagg gggcaatatc gaagaagtcc aagaaaaaga gtcaggcaaa catgattgag aaagagccag tgcaaggctg ccgcgacttc cctcccgagg acatgcgcgt tcgcaggtac ctcttcgacg tcttccacga aacggcccga aaattcggct tcgaggagta cgatgcccca gttctagagt cggaggagct ctacatccgc aaggcaggag aggagattac ggagcaaatg ttcaactttg tgacaaaagg tggccaccgt gttacacttc gcccggagat gacgccgtcg ctggtgcgtc tgcagctcgc caagggccgc tctctgcttc tgcctgctaa atggtactcc attccgcagt gctggcgtta cgaagccatc tcccgcggcc gccgccgtga gcactaccag tggaatatgg atatcgtcgg cgtgaagagc gttgccgcag aggttgagct tgtgtgtgcg gcgtgcgcgg cgatggaggc gcttggcctc accgcgcaag atgtcggcgt gaaggttaac tcccgcagat tgctgcagtg tgcactcgcc ggcgccggcg tgccagattc tctctttgct ccggtgtgcg tgattgtcga caagatggag aagctgcctg aggagcaaat ccgcgcggag ttgaaggacc tgggcttgga tggccagcag gcggatgaca tcatccggac acttagtctc aagtctgtcg acgacttcga taaggacctc tttgataaga acggcgccct ctcggagatt gcggagttct tctcgcagat acagatgtac gggtacggcg actggatcca gttcgatgcg agtgttgtgc gtggacttgc ctattacact gggatcgtct tcgaagcgtt tgaccgaagt ggtgagttcc gcgcgatctg cggaggcggc cgctacgaca acctcttcac cctgtacggc agcctgcagc ctgtcccgtg cgtcgggttt ggctttggcg actgtgtcat cgttgaactt ctgaagaaga agaagttgat caactcgctg ccacataagg tggatgatgt ggtgattccg ttcaatgagt ctcagcgagg caccgccctg aaggtactga agcagctgcg tgacaagggc cgcagtgtag acatcattat ggaccccaag aaggttgctc agaccttcag ttacgccgat cgtatcggcg ctgaccgtgc cgttctgatt gcgccagacg aaatggagaa gggcatggta cgcgtcaaga tgctgcgtga aggacaaagg ggcgagaatg accgcggtga ccttgtgccc ttcgaggact tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat