LepnA.19523.a

ATP phosphoribosyltransferase

CENTER ID:	LepnA.19523.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.19523.a.B1.GE41649	Full length(LepnA.19523.a)		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272624.6.peg.1262
UniProt:	Q5ZW86

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFKLWYRRLS FNSRKIGEVV KNRLRLALQK KGRLSDESLN LLQRCGLKFR IKPNALLTHV DNFPIDLLFV RDDDIPTLVF DSLCDGGIVG ENVLLEASFN RPDKAYRKIL ALGSCTCRLS IAVPEAFDYQ GAGSLDGKRI ATSYPNLLNN YLAERKISAE ILTLSGSVEV APRMGMADVI CDLVSTGQTL EDNKLKEVDT VLNSQAVFIQ TQRQLDPDIQ ELFAMLARRI QAVQQAQERK YIVFHAPKSA LERIAQKLPG AESPTILPLP GNNEKVAVHV VSSEGVFWNT LETIQYLGAS SILVLPIEKM LE

NT Sequence

TTGTTTAAGC TTTGGTACAG GCGGTTATCG TTTAATAGCC GAAAGATTGG AGAAGTTGTG AAAAATCGTT TACGTCTGGC CTTACAAAAA AAAGGCCGAT TATCAGATGA ATCTTTAAAT CTCCTACAAC GCTGTGGTTT AAAGTTTCGT ATTAAACCAA ACGCATTATT AACTCATGTT GATAATTTTC CAATCGATTT ACTATTTGTT CGCGATGATG ACATCCCTAC TCTCGTATTC GACAGTTTAT GTGATGGTGG CATCGTGGGA GAAAATGTGC TGCTGGAAGC TTCTTTTAAC AGACCAGACA AAGCCTATAG AAAAATATTG GCATTAGGCA GTTGTACTTG TCGTTTATCG ATAGCAGTAC CAGAAGCATT CGACTATCAA GGAGCCGGTA GTTTGGATGG GAAGCGAATT GCAACCAGCT ATCCAAATTT ACTCAACAAT TATCTTGCTG AACGAAAAAT TAGTGCGGAA ATTCTGACAT TATCCGGCTC GGTTGAGGTA GCGCCACGAA TGGGAATGGC TGATGTCATA TGCGACTTGG TATCCACTGG CCAAACACTC GAAGATAACA AACTCAAAGA AGTCGATACT GTTCTTAACA GTCAGGCCGT CTTTATTCAA ACTCAGAGAC AATTGGATCC TGATATTCAA GAGTTGTTTG CCATGCTTGC CAGAAGAATC CAGGCTGTCC AGCAAGCTCA GGAAAGAAAA TACATAGTTT TTCATGCGCC CAAATCCGCC CTTGAGCGAA TTGCTCAGAA ATTACCAGGC GCTGAGTCCC CAACCATTCT TCCATTACCG GGAAACAATG AAAAAGTTGC AGTGCATGTA GTCTCCAGTG AAGGTGTGTT TTGGAATACG TTAGAGACAA TTCAATATTT AGGTGCCAGC TCCATCCTGG TATTACCCAT TGAAAAAATG CTGGAG

Details for LepnA.19523.a.B1.GE41649

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML FKLWYRRLSF NSRKIGEVVK NRLRLALQKK GRLSDESLNL LQRCGLKFRI KPNALLTHVD NFPIDLLFVR DDDIPTLVFD SLCDGGIVGE NVLLEASFNR PDKAYRKILA LGSCTCRLSI AVPEAFDYQG AGSLDGKRIA TSYPNLLNNY LAERKISAEI LTLSGSVEVA PRMGMADVIC DLVSTGQTLE DNKLKEVDTV LNSQAVFIQT QRQLDPDIQE LFAMLARRIQ AVQQAQERKY IVFHAPKSAL ERIAQKLPGA ESPTILPLPG NNEKVAVHVV SSEGVFWNTL ETIQYLGASS IXXXXXXKML E

Validated NT Sequence

ttactccagc attttttnnn nnnnnnnnnn nnggatggag ctggcaccta aatattgaat tgtctctaac gtattccaaa acacaccttc actggagact acatgcactg caactttttc attgtttccc ggtaatggaa gaatggttgg ggactcagcg cctggtaatt tctgagcaat tcgctcaagg gcggatttgg gcgcatgaaa aactatgtat tttctttcct gagcttgctg gacagcctgg attcttctgg caagcatggc aaacaactct tgaatatcag gatccaattg tctctgagtt tgaataaaga cggcctgact gttaagaaca gtatcgactt ctttgagttt gttatcttcg agtgtttggc cagtggatac caagtcgcat atgacatcag ccattcccat tcgtggcgct acctcaaccg agccggataa tgtcagaatt tccgcactaa tttttcgttc agcaagataa ttgttgagta aatttggata gctggttgca attcgcttcc catccaaact accggctcct tgatagtcga atgcttctgg tactgctatc gataaacgac aagtacaact gcctaatgcc aatatttttc tataggcttt gtctggtctg ttaaaagaag cttccagcag cacattttct cccacgatgc caccatcaca taaactgtcg aatacgagag tagggatgtc atcatcgcga acaaatagta aatcgattgg aaaattatca acatgagtta ataatgcgtt tggtttaata cgaaacttta aaccacagcg ttgtaggaga tttaaagatt catctgataa tcggcctttt ttttgtaagg ccagacgtaa acgatttttc acaacttctc caatctttcg gctattaaac gataaccgcc tgtaccaaag cttaaacaac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMF KLWYRRLSFN SRKIGEVVKN RLRLALQKKG RLSDESLNLL QRCGLKFRIK PNALLTHVDN FPIDLLFVRD DDIPTLVFDS LCDGGIVGEN VLLEASFNRP DKAYRKILAL GSCTCRLSIA VPEAFDYQGA GSLDGKRIAT SYPNLLNNYL AERKISAEIL TLSGSVEVAP RMGMADVICD LVSTGQTLED NKLKEVDTVL NSQAVFIQTQ RQLDPDIQEL FAMLARRIQA VQQAQERKYI VFHAPKSALE RIAQKLPGAE SPTILPLPGN NEKVAVHVVS SEGVFWNTLE TIQYLGASSI LVLPIEKMLE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgttta agctttggta caggcggtta tcgtttaata gccgaaagat tggagaagtt gtgaaaaatc gtttacgtct ggccttacaa aaaaaaggcc gattatcaga tgaatcttta aatctcctac aacgctgtgg tttaaagttt cgtattaaac caaacgcatt attaactcat gttgataatt ttccaatcga tttactattt gttcgcgatg atgacatccc tactctcgta ttcgacagtt tatgtgatgg tggcatcgtg ggagaaaatg tgctgctgga agcttctttt aacagaccag acaaagccta tagaaaaata ttggcattag gcagttgtac ttgtcgttta tcgatagcag taccagaagc attcgactat caaggagccg gtagtttgga tgggaagcga attgcaacca gctatccaaa tttactcaac aattatcttg ctgaacgaaa aattagtgcg gaaattctga cattatccgg ctcggttgag gtagcgccac gaatgggaat ggctgatgtc atatgcgact tggtatccac tggccaaaca ctcgaagata acaaactcaa agaagtcgat actgttctta acagtcaggc cgtctttatt caaactcaga gacaattgga tcctgatatt caagagttgt ttgccatgct tgccagaaga atccaggctg tccagcaagc tcaggaaaga aaatacatag tttttcatgc gcccaaatcc gcccttgagc gaattgctca gaaattacca ggcgctgagt ccccaaccat tcttccatta ccgggaaaca atgaaaaagt tgcagtgcat gtagtctcca gtgaaggtgt gttttggaat acgttagaga caattcaata tttaggtgcc agctccatcc tggtattacc cattgaaaaa atgctggagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat