LepnA.19522.a
Urocanate hydratase
| CENTER ID: | LepnA.19522.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.19522.a.B1.GE41648 | Full length(LepnA.19522.a) | 1 | 555 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.19522.a.B1.PW38395 | Full length(LepnA.19522.a) | 1 | 555 |
Structures
7JFZ
DEPOSITED: 7/17/2020
DETERMINATION: XRay
CLONE: LepnA.19522.a.B1.GE41648
PROTEIN: LepnA.19522.a.B1.PW38395
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.1449 |
| UniProt: | Q5ZVR1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMNKDKNKRV ISAPRGTEIQ AKNWLTEAAL RMICNNLDPN VAEDPDSLIV YGGLGKAARN WECFDEIVHV LKLLNNDQTL LIQSGKPVGV FTTHEEAPRI LIANSNLVPR WATWEHFNEL DKKGLMMYGQ MTAGSWIYIG SQGIVQGTYE TFVAAAKKHY QGDLSGRWIL TAGLGGMGGA QPLAGTMAGA SVLAVECDRQ RIEKRLQTKY LDRYTDNLSE ALDWINESCR RKKPVSVAVL GNAAEIFPQL VKLGVQPSLV TDQTSAHDPL NGYLPLGWTL EQAVEMRKKS PEEVVDAAKK SMAVQVHAML EFHNRGIPVF DYGNNIRQMA FEAGEKNAFS FEGFVPAYIR PLFCEGIGPF RWVALSGDPE DIYATDERVK QLIPDAPHLH HWLDMAREKI SFQGLPARIC WVGLKDRARL ALAFNEMVKN KQVKAPIVIG RDHLDSGSVA SPNRETEGML DGSDAVSDWP LLNALLNCAS GATWVSIHHG GGVGMGFSQH AGVVIVADGT EKAAKRLARV LHNDPATGVM RHADAGYQIA KQCAKENSLW LPMES
NT Sequence
TTGATGAACA AGGACAAGAA TAAACGTGTT ATTTCTGCCC CTAGAGGTAC CGAAATTCAA GCTAAAAATT GGTTAACTGA GGCGGCATTA AGGATGATTT GCAATAATTT GGATCCCAAT GTTGCAGAGG ATCCCGATTC TTTGATTGTT TATGGTGGAT TGGGCAAGGC AGCCAGAAAC TGGGAATGTT TTGATGAAAT TGTTCATGTA TTGAAATTAT TAAATAATGA TCAGACTTTA CTCATTCAGT CCGGAAAACC TGTCGGTGTA TTTACTACCC ATGAAGAGGC TCCCAGAATA CTAATTGCCA ATTCTAATTT GGTACCGCGC TGGGCTACTT GGGAGCATTT TAACGAACTG GATAAGAAAG GCCTGATGAT GTATGGCCAG ATGACCGCCG GCAGCTGGAT TTATATTGGT TCTCAAGGCA TTGTGCAAGG AACTTATGAG ACATTTGTTG CGGCAGCTAA AAAACATTAT CAGGGTGATT TGTCCGGGCG TTGGATTTTA ACAGCGGGTT TAGGGGGAAT GGGGGGAGCT CAACCCTTGG CTGGAACTAT GGCTGGTGCC AGCGTTCTTG CTGTAGAATG TGACAGACAA AGGATTGAGA AACGTTTACA GACAAAGTAT CTGGATCGGT ACACTGATAA TTTAAGTGAA GCATTAGACT GGATTAATGA ATCCTGTCGC CGTAAAAAGC CAGTTTCAGT AGCTGTTTTG GGAAATGCTG CTGAAATATT TCCTCAACTG GTTAAGTTGG GAGTGCAGCC TTCTCTGGTT ACCGATCAAA CCAGCGCTCA TGATCCGCTC AATGGCTATT TGCCATTAGG ATGGACATTA GAACAAGCTG TTGAAATGAG AAAAAAATCA CCAGAAGAGG TTGTTGATGC AGCCAAGAAA TCGATGGCTG TTCAAGTGCA TGCAATGCTT GAATTTCATA ATAGAGGCAT TCCTGTATTT GATTATGGCA ATAACATCAG ACAAATGGCA TTTGAAGCGG GAGAAAAAAA CGCTTTTTCC TTTGAGGGAT TTGTACCTGC TTATATCAGG CCATTATTTT GCGAAGGAAT TGGACCATTT CGATGGGTTG CATTGTCAGG TGATCCAGAA GATATTTATG CAACGGATGA GCGAGTAAAG CAGTTAATTC CTGACGCTCC TCATTTGCAT CATTGGTTAG ATATGGCCAG GGAAAAAATT TCATTTCAGG GATTACCAGC AAGGATATGT TGGGTAGGGT TAAAGGATAG AGCACGCCTT GCCCTGGCTT TCAATGAAAT GGTTAAAAAT AAACAAGTTA AAGCGCCTAT TGTGATTGGC CGTGACCATT TGGATTCTGG TTCTGTGGCT AGCCCCAATC GTGAAACAGA AGGTATGCTG GATGGCAGTG ATGCTGTTTC TGATTGGCCT TTGCTTAATG CGCTGTTAAA TTGTGCCAGT GGGGCTACTT GGGTAAGTAT TCACCACGGA GGAGGGGTTG GTATGGGCTT TTCGCAACAT GCTGGCGTTG TGATTGTTGC TGATGGGACA GAAAAGGCAG CTAAACGATT AGCCCGTGTG TTACACAATG ATCCTGCGAC AGGGGTAATG AGGCATGCTG ATGCAGGTTA TCAAATTGCC AAGCAATGTG CCAAAGAGAA CTCGCTTTGG CTACCCATGG AATCT
Details for LepnA.19522.a.B1.GE41648
| HARVESTED ON: | 6/23/2017 |
| SEQUENCED ON: | 6/28/2017 |
| EXPECTED MW: | 62kDa |
| OBSERVED MW: | 62kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 57 |
Validated AA Sequence
MAHHHHHHML MNKDKNKRVI SAPRGTEIQA KNWLTEAALR MICNNLDPNV AEDPDSLIVY GGLGKAARNW ECFDEIVHVL KLLNNDQTLL IQSGKPVGVF TTHEEAPRIL IANSNLVPRW ATWEHFNELD KKGLMMYGQM TAGSWIYIGS QGIVQGTYET FVAAAKKHYQ GDLSGRWILT AGLGGMGGAQ PLAGTMAGAS VLAVECDRQR IEKRLQTKYL DRYTDNXXEX LDWINESCXR KKPVXXAVLX NAAEIXXXXX XXGXXXSLVX XQTXXXXXXX XXXXXXMDIR TSXXNXXKXX RRXXXCSQEX XXCSSACNXX IS
Validated NT Sequence
gttctctttg gcacattgct tggcaatttg ataacctgca tcagcatgcc tcattacccc tgtcgcagga tcattgtgta acacacgggc taatcgttta gctgcctttt ctgtcccatc agcaacaatc acaacgccag catgttgcga aaagcccata ccaacccctc ctccgtggtg aatacttacc caagtagccc cactggcaca atttaacagc gcattaagca aaggccaatc agaaacagca tcactgccat ccagcatacc ttctgtttca cgattggggc tagccacaga accagaatcc aaatggtcac ggccaatcac aataggcgct ttaacttgtt tatttttaac catttcattg aaagccaggg caaggcgtgc tctatccttt aaccctaccc aacatatcct tgctggtaat ccctgaaatg aaattttttc cctggccata tctaaccaat gatgcaaatg aggagcgtca ggaattaact gctttactcg ctcatccgtt gcataaatat cttctggatc acctgacaat gcaacccatc gaaatggtcc aattccttcg caaaataatg gcctgatata agcaggtann nntccctcaa aggaaaaagc gtttttttct cccgcttcaa atgccatttg tctgatgtta ttgccataat caaatacagg aatgcctcta ttatgaaatt nnnncattgc atgcacttga acagnnntnn ntttcttggc tgcatnnnnn nnntcttctg nnnnnttttt nnncattnnn nnngcttgtt ctaatgtcca tcnnnnnnnn nnnnnnnnat nnnnnnngan nnnnnnnnnn ggtttgatnn nnnaccagag annnnnnnnc tccnnnnnnn nnnnnnnnnn nnaatatttc agcagcattn nccaaaacag ctnnnnaaac tggcttttta cggcnacagg attcattaat ccagtctaat ncttcannna aattatcagt gtaccgatcc agatactttg tctgtaaacg tttctcaatc ctttgtctgt cacattctac agcaagaacg ctggcaccag ccatagttcc agccaagggt tgagctcccc ccattccccc taaacccgct gttaaaatcc aacgcccgga caaatcaccc tgataatgtt ttttagctgc cgcaacaaat gtctcataag ttccttgcac aatgccttga gaaccaatat aaatccagct gccggcggtc atctggccat acatcatcag gcctttctta tccagttcgt taaaatgctc ccaagtagcc cagcgcggta ccaaattaga attggcaatt agtattctgg gagcctcttc atgggtagta aatacaccga caggttttcc ggactgaatg agtaaagtct gatcattatt taataatttc aatacatgaa caatttcatc aaaacattcc cagtttctgg ctgccttgcc caatccacca taaacaatca aagaatcggg atcctctgca acattgggat ccaaattatt gcaaatcatc cttaatgccg cctcagttaa ccaattttta gcttgaattt cggtacctct aggggcagaa ataacacgtt tattcttgtc cttgttcatc aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMM NKDKNKRVIS APRGTEIQAK NWLTEAALRM ICNNLDPNVA EDPDSLIVYG GLGKAARNWE CFDEIVHVLK LLNNDQTLLI QSGKPVGVFT THEEAPRILI ANSNLVPRWA TWEHFNELDK KGLMMYGQMT AGSWIYIGSQ GIVQGTYETF VAAAKKHYQG DLSGRWILTA GLGGMGGAQP LAGTMAGASV LAVECDRQRI EKRLQTKYLD RYTDNLSEAL DWINESCRRK KPVSVAVLGN AAEIFPQLVK LGVQPSLVTD QTSAHDPLNG YLPLGWTLEQ AVEMRKKSPE EVVDAAKKSM AVQVHAMLEF HNRGIPVFDY GNNIRQMAFE AGEKNAFSFE GFVPAYIRPL FCEGIGPFRW VALSGDPEDI YATDERVKQL IPDAPHLHHW LDMAREKISF QGLPARICWV GLKDRARLAL AFNEMVKNKQ VKAPIVIGRD HLDSGSVASP NRETEGMLDG SDAVSDWPLL NALLNCASGA TWVSIHHGGG VGMGFSQHAG VVIVADGTEK AAKRLARVLH NDPATGVMRH ADAGYQIAKQ CAKENSLWLP MES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgatga acaaggacaa gaataaacgt gttatttctg cccctagagg taccgaaatt caagctaaaa attggttaac tgaggcggca ttaaggatga tttgcaataa tttggatccc aatgttgcag aggatcccga ttctttgatt gtttatggtg gattgggcaa ggcagccaga aactgggaat gttttgatga aattgttcat gtattgaaat tattaaataa tgatcagact ttactcattc agtccggaaa acctgtcggt gtatttacta cccatgaaga ggctcccaga atactaattg ccaattctaa tttggtaccg cgctgggcta cttgggagca ttttaacgaa ctggataaga aaggcctgat gatgtatggc cagatgaccg ccggcagctg gatttatatt ggttctcaag gcattgtgca aggaacttat gagacatttg ttgcggcagc taaaaaacat tatcagggtg atttgtccgg gcgttggatt ttaacagcgg gtttaggggg aatgggggga gctcaaccct tggctggaac tatggctggt gccagcgttc ttgctgtaga atgtgacaga caaaggattg agaaacgttt acagacaaag tatctggatc ggtacactga taatttaagt gaagcattag actggattaa tgaatcctgt cgccgtaaaa agccagtttc agtagctgtt ttgggaaatg ctgctgaaat atttcctcaa ctggttaagt tgggagtgca gccttctctg gttaccgatc aaaccagcgc tcatgatccg ctcaatggct atttgccatt aggatggaca ttagaacaag ctgttgaaat gagaaaaaaa tcaccagaag aggttgttga tgcagccaag aaatcgatgg ctgttcaagt gcatgcaatg cttgaatttc ataatagagg cattcctgta tttgattatg gcaataacat cagacaaatg gcatttgaag cgggagaaaa aaacgctttt tcctttgagg gatttgtacc tgcttatatc aggccattat tttgcgaagg aattggacca tttcgatggg ttgcattgtc aggtgatcca gaagatattt atgcaacgga tgagcgagta aagcagttaa ttcctgacgc tcctcatttg catcattggt tagatatggc cagggaaaaa atttcatttc agggattacc agcaaggata tgttgggtag ggttaaagga tagagcacgc cttgccctgg ctttcaatga aatggttaaa aataaacaag ttaaagcgcc tattgtgatt ggccgtgacc atttggattc tggttctgtg gctagcccca atcgtgaaac agaaggtatg ctggatggca gtgatgctgt ttctgattgg cctttgctta atgcgctgtt aaattgtgcc agtggggcta cttgggtaag tattcaccac ggaggagggg ttggtatggg cttttcgcaa catgctggcg ttgtgattgt tgctgatggg acagaaaagg cagctaaacg attagcccgt gtgttacaca atgatcctgc gacaggggta atgaggcatg ctgatgcagg ttatcaaatt gccaagcaat gtgccaaaga gaactcgctt tggctaccca tggaatcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LepnA.19522.a.B1.PW38395
| PURIFICATION DATe: | 11/27/2017 |
| CONCENTRATION: | 25.57mg/ml |
| OBSERVED MW: | 62kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 80 |
| PERCENT COVERAGE: | 57 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML MNKDKNKRVI SAPRGTEIQA KNWLTEAALR MICNNLDPNV AEDPDSLIVY GGLGKAARNW ECFDEIVHVL KLLNNDQTLL IQSGKPVGVF TTHEEAPRIL IANSNLVPRW ATWEHFNELD KKGLMMYGQM TAGSWIYIGS QGIVQGTYET FVAAAKKHYQ GDLSGRWILT AGLGGMGGAQ PLAGTMAGAS VLAVECDRQR IEKRLQTKYL DRYTDNXXEX LDWINESCXR KKPVXXAVLX NAAEIXXXXX XXGXXXSLVX XQTXXXXXXX XXXXXXMDIR TSXXNXXKXX RRXXXCSQEX XXCSSACNXX IS
Validated NT Sequence
gttctctttg gcacattgct tggcaatttg ataacctgca tcagcatgcc tcattacccc tgtcgcagga tcattgtgta acacacgggc taatcgttta gctgcctttt ctgtcccatc agcaacaatc acaacgccag catgttgcga aaagcccata ccaacccctc ctccgtggtg aatacttacc caagtagccc cactggcaca atttaacagc gcattaagca aaggccaatc agaaacagca tcactgccat ccagcatacc ttctgtttca cgattggggc tagccacaga accagaatcc aaatggtcac ggccaatcac aataggcgct ttaacttgtt tatttttaac catttcattg aaagccaggg caaggcgtgc tctatccttt aaccctaccc aacatatcct tgctggtaat ccctgaaatg aaattttttc cctggccata tctaaccaat gatgcaaatg aggagcgtca ggaattaact gctttactcg ctcatccgtt gcataaatat cttctggatc acctgacaat gcaacccatc gaaatggtcc aattccttcg caaaataatg gcctgatata agcaggtann nntccctcaa aggaaaaagc gtttttttct cccgcttcaa atgccatttg tctgatgtta ttgccataat caaatacagg aatgcctcta ttatgaaatt nnnncattgc atgcacttga acagnnntnn ntttcttggc tgcatnnnnn nnntcttctg nnnnnttttt nnncattnnn nnngcttgtt ctaatgtcca tcnnnnnnnn nnnnnnnnat nnnnnnngan nnnnnnnnnn ggtttgatnn nnnaccagag annnnnnnnc tccnnnnnnn nnnnnnnnnn nnaatatttc agcagcattn nccaaaacag ctnnnnaaac tggcttttta cggcnacagg attcattaat ccagtctaat ncttcannna aattatcagt gtaccgatcc agatactttg tctgtaaacg tttctcaatc ctttgtctgt cacattctac agcaagaacg ctggcaccag ccatagttcc agccaagggt tgagctcccc ccattccccc taaacccgct gttaaaatcc aacgcccgga caaatcaccc tgataatgtt ttttagctgc cgcaacaaat gtctcataag ttccttgcac aatgccttga gaaccaatat aaatccagct gccggcggtc atctggccat acatcatcag gcctttctta tccagttcgt taaaatgctc ccaagtagcc cagcgcggta ccaaattaga attggcaatt agtattctgg gagcctcttc atgggtagta aatacaccga caggttttcc ggactgaatg agtaaagtct gatcattatt taataatttc aatacatgaa caatttcatc aaaacattcc cagtttctgg ctgccttgcc caatccacca taaacaatca aagaatcggg atcctctgca acattgggat ccaaattatt gcaaatcatc cttaatgccg cctcagttaa ccaattttta gcttgaattt cggtacctct aggggcagaa ataacacgtt tattcttgtc cttgttcatc aacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMM NKDKNKRVIS APRGTEIQAK NWLTEAALRM ICNNLDPNVA EDPDSLIVYG GLGKAARNWE CFDEIVHVLK LLNNDQTLLI QSGKPVGVFT THEEAPRILI ANSNLVPRWA TWEHFNELDK KGLMMYGQMT AGSWIYIGSQ GIVQGTYETF VAAAKKHYQG DLSGRWILTA GLGGMGGAQP LAGTMAGASV LAVECDRQRI EKRLQTKYLD RYTDNLSEAL DWINESCRRK KPVSVAVLGN AAEIFPQLVK LGVQPSLVTD QTSAHDPLNG YLPLGWTLEQ AVEMRKKSPE EVVDAAKKSM AVQVHAMLEF HNRGIPVFDY GNNIRQMAFE AGEKNAFSFE GFVPAYIRPL FCEGIGPFRW VALSGDPEDI YATDERVKQL IPDAPHLHHW LDMAREKISF QGLPARICWV GLKDRARLAL AFNEMVKNKQ VKAPIVIGRD HLDSGSVASP NRETEGMLDG SDAVSDWPLL NALLNCASGA TWVSIHHGGG VGMGFSQHAG VVIVADGTEK AAKRLARVLH NDPATGVMRH ADAGYQIAKQ CAKENSLWLP MES
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGATGA ACAAGGACAA GAATAAACGT GTTATTTCTG CCCCTAGAGG TACCGAAATT CAAGCTAAAA ATTGGTTAAC TGAGGCGGCA TTAAGGATGA TTTGCAATAA TTTGGATCCC AATGTTGCAG AGGATCCCGA TTCTTTGATT GTTTATGGTG GATTGGGCAA GGCAGCCAGA AACTGGGAAT GTTTTGATGA AATTGTTCAT GTATTGAAAT TATTAAATAA TGATCAGACT TTACTCATTC AGTCCGGAAA ACCTGTCGGT GTATTTACTA CCCATGAAGA GGCTCCCAGA ATACTAATTG CCAATTCTAA TTTGGTACCG CGCTGGGCTA CTTGGGAGCA TTTTAACGAA CTGGATAAGA AAGGCCTGAT GATGTATGGC CAGATGACCG CCGGCAGCTG GATTTATATT GGTTCTCAAG GCATTGTGCA AGGAACTTAT GAGACATTTG TTGCGGCAGC TAAAAAACAT TATCAGGGTG ATTTGTCCGG GCGTTGGATT TTAACAGCGG GTTTAGGGGG AATGGGGGGA GCTCAACCCT TGGCTGGAAC TATGGCTGGT GCCAGCGTTC TTGCTGTAGA ATGTGACAGA CAAAGGATTG AGAAACGTTT ACAGACAAAG TATCTGGATC GGTACACTGA TAATTTAAGT GAAGCATTAG ACTGGATTAA TGAATCCTGT CGCCGTAAAA AGCCAGTTTC AGTAGCTGTT TTGGGAAATG CTGCTGAAAT ATTTCCTCAA CTGGTTAAGT TGGGAGTGCA GCCTTCTCTG GTTACCGATC AAACCAGCGC TCATGATCCG CTCAATGGCT ATTTGCCATT AGGATGGACA TTAGAACAAG CTGTTGAAAT GAGAAAAAAA TCACCAGAAG AGGTTGTTGA TGCAGCCAAG AAATCGATGG CTGTTCAAGT GCATGCAATG CTTGAATTTC ATAATAGAGG CATTCCTGTA TTTGATTATG GCAATAACAT CAGACAAATG GCATTTGAAG CGGGAGAAAA AAACGCTTTT TCCTTTGAGG GATTTGTACC TGCTTATATC AGGCCATTAT TTTGCGAAGG AATTGGACCA TTTCGATGGG TTGCATTGTC AGGTGATCCA GAAGATATTT ATGCAACGGA TGAGCGAGTA AAGCAGTTAA TTCCTGACGC TCCTCATTTG CATCATTGGT TAGATATGGC CAGGGAAAAA ATTTCATTTC AGGGATTACC AGCAAGGATA TGTTGGGTAG GGTTAAAGGA TAGAGCACGC CTTGCCCTGG CTTTCAATGA AATGGTTAAA AATAAACAAG TTAAAGCGCC TATTGTGATT GGCCGTGACC ATTTGGATTC TGGTTCTGTG GCTAGCCCCA ATCGTGAAAC AGAAGGTATG CTGGATGGCA GTGATGCTGT TTCTGATTGG CCTTTGCTTA ATGCGCTGTT AAATTGTGCC AGTGGGGCTA CTTGGGTAAG TATTCACCAC GGAGGAGGGG TTGGTATGGG CTTTTCGCAA CATGCTGGCG TTGTGATTGT TGCTGATGGG ACAGAAAAGG CAGCTAAACG ATTAGCCCGT GTGTTACACA ATGATCCTGC GACAGGGGTA ATGAGGCATG CTGATGCAGG TTATCAAATT GCCAAGCAAT GTGCCAAAGA GAACTCGCTT TGGCTACCCA TGGAATCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT