SSGCID: Target Page

LepnA.18851.a

Electron transferring flavoprotein dehydrogenase

CENTER ID:	LepnA.18851.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.18851.a.B1.GE41646	Full length(LepnA.18851.a)		1	543

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.18851.a.B1.PS38454	Full length(LepnA.18851.a)		1	543

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272624.6.peg.1344
UniProt:	Q5ZW12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEHETMEFDV IIVGAGPSGL SAAIKLKQLA LASQKEITIC ILEKGAQVGA HILSGAVLEP RSLKELLPDN WQQAPLDTPV NKDLFYFLTQ NKSFKLPTPK PMHNEGNFII SLGELCKFLA EQAESLGCEI YPGFAAAEIL YNDKNQVIGV ATGDVGIDRQ GKKTDNYQPG MHLLAKQTLF AEGCRGQLSQ TLMSRYHLRD HSQPQTYGIG IKEIWQVDAS RHEPGKVIHT VGWPLDHATY GGSFLYHLSE QRVAIGFVVG LDYKNPWLNP FGEFQRFKTH PLIKSVLTGG ERIGYGARAL NEGGWQSLPK LTFPGGALIG DAAGFLNVPK IKGIHTAMQS GMLAAQACFD ALSEEKSGQF ELSAYPKKIN QSWLKQELYA VRNIRPGFKY GLFPGLINAA FETYVTRGYS PWTLKNHSDY KSLIPADKAK KINYPKPDGV LTFDKLSSVF LSNTFHEENQ PCHLKLKQPK LAIEVNLNQY ASPESRYCPA AVYEIIEDVE KGPRLQINAQ NCIHCKTCDI KDPRQNIVWQ APEGGGGPNY SGM

NT Sequence

GTGGAACACG AAACGATGGA ATTTGATGTT ATCATAGTCG GTGCAGGGCC TTCTGGATTA TCCGCTGCAA TCAAATTAAA GCAACTGGCT TTAGCTTCAC AAAAAGAAAT TACTATTTGT ATCCTGGAAA AAGGAGCTCA GGTTGGCGCT CATATTCTTT CTGGCGCAGT TTTAGAGCCT AGAAGCTTAA AAGAATTACT ACCAGATAAT TGGCAACAGG CCCCACTGGA TACTCCTGTT AACAAAGATC TGTTTTATTT TTTGACTCAA AATAAATCAT TTAAGCTCCC TACTCCAAAA CCTATGCATA ATGAAGGAAA TTTTATTATC AGTTTAGGGG AACTATGTAA GTTTCTTGCA GAACAAGCAG AGTCTCTAGG CTGTGAAATA TACCCTGGAT TTGCTGCAGC AGAAATTCTC TACAATGATA AAAATCAAGT CATAGGTGTT GCTACTGGGG ATGTAGGAAT TGATAGACAA GGGAAAAAAA CTGATAATTA TCAACCTGGA ATGCATTTAT TAGCCAAACA AACTTTATTT GCTGAGGGAT GTCGCGGGCA ATTAAGTCAA ACATTAATGA GTCGCTATCA TTTAAGAGAT CATTCGCAGC CACAAACTTA CGGCATAGGG ATTAAAGAAA TCTGGCAAGT TGATGCATCG CGCCATGAAC CTGGAAAAGT TATCCATACG GTTGGTTGGC CTCTTGATCA TGCGACTTAT GGAGGCTCCT TTTTGTATCA TCTTTCAGAG CAGAGAGTTG CAATAGGTTT TGTTGTTGGG CTTGACTACA AGAACCCATG GCTCAATCCC TTTGGCGAAT TTCAGAGATT TAAAACGCAT CCCCTTATTA AATCTGTTCT AACAGGAGGT GAGCGAATTG GTTATGGAGC CAGAGCACTT AATGAAGGAG GATGGCAATC TCTCCCTAAA CTTACCTTTC CTGGCGGCGC CTTGATTGGA GATGCTGCCG GTTTCCTAAA TGTGCCTAAA ATCAAAGGAA TTCACACCGC AATGCAATCT GGAATGCTTG CTGCACAAGC TTGCTTTGAT GCGCTAAGTG AAGAAAAGAG CGGTCAATTT GAACTAAGTG CATACCCTAA GAAAATCAAT CAATCCTGGC TAAAGCAAGA GCTCTATGCA GTGCGAAATA TTCGACCAGG ATTCAAATAC GGTTTATTTC CAGGTCTAAT TAATGCTGCC TTCGAGACTT ATGTGACTCG AGGATATTCT CCATGGACCT TAAAAAATCA TTCTGACTAT AAGTCGTTAA TTCCAGCAGA CAAAGCTAAA AAAATTAACT ACCCAAAACC AGATGGCGTA CTCACGTTTG ATAAACTCTC ATCTGTATTT TTATCAAATA CATTTCATGA GGAAAATCAA CCCTGTCATC TCAAATTAAA ACAACCCAAG CTGGCCATTG AAGTGAACTT AAACCAATAC GCTTCTCCGG AAAGTCGATA CTGCCCGGCT GCTGTTTATG AAATAATTGA GGACGTTGAA AAAGGACCTC GATTACAAAT TAACGCCCAG AATTGTATTC ATTGTAAAAC GTGCGATATT AAGGATCCTC GTCAAAATAT AGTTTGGCAA GCACCAGAAG GTGGAGGTGG ACCGAATTAT TCAGGAATG

Details for LepnA.18851.a.B1.GE41646

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME HETMEFDVII VGAGPSGLSA AIKLKQLALA SQKEITICIL EKGAQVGAHI LSGAVLEPRS LKELLPDNWQ QAPLDTPVNK DLFYFLTQNK SFKLPTPKPM HNEGNFIISL GELCKFLAEQ AESLGCEIYP GFAAAEILYN DKNQVIGVAT GDVGIDRQGK KTDNYQPGMH LLAKQTLFAE GCRGQLSQTL MSRYHLRDHS QPQTYGIGIK EIWQVDASRH EPGKVIHTVG WPLDHATYGG SFLYHLSEQR VAIGFVVGLD YKNPWLNPFG EFQRFKTHPL IKSVLTGGER IGYGARALNE GGWQSLPKLT FPGGALIGDA AGFLNVPKIK GIHTAMQSGM LAAQACFDAL SEEKSGQFEL SAYPKKINQS WLKQELYAVR NIRPGFKYGL FPGLINAAFE TYVTRGYSPW TLKNHSDYKS LIPADKAKKI NYPKPDGVLT FDKLSSVFLS NTFHEENQPC HLKLKQPKLA IEVNLNQYAS PESRYCPAAV YEIIEDVEKG PRLQINAQNC IHCKTCDIKD PRQNIVWQAP EGGGGPNYSG M

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggaac acgaaacgat ggaatttgat gttatcatag tcggtgcagg gccttctgga ttatccgctg caatcaaatt aaagcaactg gctttagctt cacaaaaaga aattactatt tgtatcctgg aaaaaggagc tcaggttggc gctcatattc tttctggcgc agttttagag cctagaagct taaaagaatt actaccagat aattggcaac aggccccact ggatactcct gttaacaaag atctgtttta ttttttgact caaaataaat catttaagct ccctactcca aaacctatgc ataatgaagg aaattttatt atcagtttag gggaactatg taagtttctt gcagaacaag cagagtctct aggctgtgaa atataccctg gatttgctgc agcagaaatt ctctacaatg ataaaaatca agtcataggt gttgctactg gggatgtagg aattgataga caagggaaaa aaactgataa ttatcaacct ggaatgcatt tattagccaa acaaacttta tttgctgagg gatgtcgcgg gcaattaagt caaacattaa tgagtcgcta tcatttaaga gatcattcgc agccacaaac ttacggcata gggattaaag aaatctggca agttgatgca tcgcgccatg aacctggaaa agttatccat acggttggtt ggcctcttga tcatgcgact tatggaggct cctttttgta tcatctttca gagcagagag ttgcaatagg ttttgttgtt gggcttgact acaagaaccc atggctcaat ccctttggcg aatttcagag atttaaaacg catcccctta ttaaatctgt tctaacagga ggtgagcgaa ttggttatgg agccagagca cttaatgaag gaggatggca atctctccct aaacttacct ttcctggcgg cgccttgatt ggagatgctg ccggtttcct aaatgtgcct aaaatcaaag gaattcacac cgcaatgcaa tctggaatgc ttgctgcaca agcttgcttt gatgcgctaa gtgaagaaaa gagcggtcaa tttgaactaa gtgcataccc taagaaaatc aatcaatcct ggctaaagca agagctctat gcagtgcgaa atattcgacc aggattcaaa tacggtttat ttccaggtct aattaatgct gccttcgaga cttatgtgac tcgaggatat tctccatgga ccttaaaaaa tcattctgac tataagtcgt taattccagc agacaaagct aaaaaaatta actacccaaa accagatggc gtactcacgt ttgataaact ctcatctgta tttttatcaa atacatttca tgaggaaaat caaccctgtc atctcaaatt aaaacaaccc aagctggcca ttgaagtgaa cttaaaccaa tacgcttctc cggaaagtcg atactgcccg gctgctgttt atgaaataat tgaggacgtt gaaaaaggac ctcgattaca aattaacgcc cagaattgta ttcattgtaa aacgtgcgat attaaggatc ctcgtcaaaa tatagtttgg caagcaccag aaggtggagg tggaccgaat tattcaggaa tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for LepnA.18851.a.B1.PS38454

PURIFICATION DATe:	5/30/2018
CONCENTRATION:	7.7mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGAAC ACGAAACGAT GGAATTTGAT GTTATCATAG TCGGTGCAGG GCCTTCTGGA TTATCCGCTG CAATCAAATT AAAGCAACTG GCTTTAGCTT CACAAAAAGA AATTACTATT TGTATCCTGG AAAAAGGAGC TCAGGTTGGC GCTCATATTC TTTCTGGCGC AGTTTTAGAG CCTAGAAGCT TAAAAGAATT ACTACCAGAT AATTGGCAAC AGGCCCCACT GGATACTCCT GTTAACAAAG ATCTGTTTTA TTTTTTGACT CAAAATAAAT CATTTAAGCT CCCTACTCCA AAACCTATGC ATAATGAAGG AAATTTTATT ATCAGTTTAG GGGAACTATG TAAGTTTCTT GCAGAACAAG CAGAGTCTCT AGGCTGTGAA ATATACCCTG GATTTGCTGC AGCAGAAATT CTCTACAATG ATAAAAATCA AGTCATAGGT GTTGCTACTG GGGATGTAGG AATTGATAGA CAAGGGAAAA AAACTGATAA TTATCAACCT GGAATGCATT TATTAGCCAA ACAAACTTTA TTTGCTGAGG GATGTCGCGG GCAATTAAGT CAAACATTAA TGAGTCGCTA TCATTTAAGA GATCATTCGC AGCCACAAAC TTACGGCATA GGGATTAAAG AAATCTGGCA AGTTGATGCA TCGCGCCATG AACCTGGAAA AGTTATCCAT ACGGTTGGTT GGCCTCTTGA TCATGCGACT TATGGAGGCT CCTTTTTGTA TCATCTTTCA GAGCAGAGAG TTGCAATAGG TTTTGTTGTT GGGCTTGACT ACAAGAACCC ATGGCTCAAT CCCTTTGGCG AATTTCAGAG ATTTAAAACG CATCCCCTTA TTAAATCTGT TCTAACAGGA GGTGAGCGAA TTGGTTATGG AGCCAGAGCA CTTAATGAAG GAGGATGGCA ATCTCTCCCT AAACTTACCT TTCCTGGCGG CGCCTTGATT GGAGATGCTG CCGGTTTCCT AAATGTGCCT AAAATCAAAG GAATTCACAC CGCAATGCAA TCTGGAATGC TTGCTGCACA AGCTTGCTTT GATGCGCTAA GTGAAGAAAA GAGCGGTCAA TTTGAACTAA GTGCATACCC TAAGAAAATC AATCAATCCT GGCTAAAGCA AGAGCTCTAT GCAGTGCGAA ATATTCGACC AGGATTCAAA TACGGTTTAT TTCCAGGTCT AATTAATGCT GCCTTCGAGA CTTATGTGAC TCGAGGATAT TCTCCATGGA CCTTAAAAAA TCATTCTGAC TATAAGTCGT TAATTCCAGC AGACAAAGCT AAAAAAATTA ACTACCCAAA ACCAGATGGC GTACTCACGT TTGATAAACT CTCATCTGTA TTTTTATCAA ATACATTTCA TGAGGAAAAT CAACCCTGTC ATCTCAAATT AAAACAACCC AAGCTGGCCA TTGAAGTGAA CTTAAACCAA TACGCTTCTC CGGAAAGTCG ATACTGCCCG GCTGCTGTTT ATGAAATAAT TGAGGACGTT GAAAAAGGAC CTCGATTACA AATTAACGCC CAGAATTGTA TTCATTGTAA AACGTGCGAT ATTAAGGATC CTCGTCAAAA TATAGTTTGG CAAGCACCAG AAGGTGGAGG TGGACCGAAT TATTCAGGAA TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT