LepnA.18123.a

Oxaloacetate decarboxylase alpha subunit

CENTER ID: LepnA.18123.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.18123.a.B1.GE41644 Full length(LepnA.18123.a) 1 602
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.481
UniProt: Q5ZYA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQEELMSKT FITDVTLRDA HQCLIATRMR TEDMLPICNK MDDVGFWAME VWGGATFDAC LRFLKEDPWS RLRQLRQMLP NTQLSMLLRG QNLLGYRNYA DDVVRAFVKL AVNNGVDVFR VFDALNDARN LKVAIDAIKS HKKHAQGAIC YTTSPVHTLD NFLELGKKLA EMGCDSIAIK DMAGLLTPTV TVELYAGLKQ ATGLPVHLHS HSTSGLASIC HYEAVLAGCN HIDTAISSFS GGASHPPTEA LVAALTDTPY DTELDLNILL EIDDYFKAVR KKYSQFESEA QNIDPRVQLY QVPGGMISNL YNQLREQNAL DKMDAVHKEI PRVRKDLGYP PLVTPTSQVV GTQAVINVLT GERYKTITNE VKLYCQGKYG TPPGKISSAL RKKAIGRTEV IEVRPGDLLP NELDQLQNEI SDLALSDEDV LLYAMFPEIG RQFLEQRKNN QLIPEPLLTQ SSAPDNSVMS EFDIILHGES YHVKVAGYGM IEHGQQSCFL WVDGVPEEVV VQHSELHDKI ERSSVNNKIG PGDITVAIPG SIIAIHVSVG DEVKAGQAVL VIEAMKMETE IKAPANGVVA EILCQKGDKV TPGQVLIRVE VS
NT Sequence
TTGAGCCAGG AAGAGCTTAT GTCTAAAACG TTTATAACAG ATGTCACCTT GCGTGATGCC CATCAATGCT TAATCGCTAC AAGAATGCGT ACTGAAGATA TGTTGCCAAT CTGTAATAAA ATGGATGATG TAGGATTTTG GGCTATGGAA GTCTGGGGAG GCGCTACTTT TGATGCCTGT TTGCGATTTT TAAAGGAAGA TCCCTGGTCA AGATTGCGTC AACTTCGCCA AATGTTACCG AATACTCAAC TCTCTATGCT GTTAAGAGGT CAAAATTTGT TAGGCTATAG GAATTATGCA GATGATGTAG TCCGAGCGTT TGTTAAATTG GCCGTGAATA ATGGAGTGGA TGTATTTCGT GTGTTTGATG CTTTAAATGA CGCGCGAAAC CTTAAAGTAG CCATAGACGC GATTAAATCA CATAAAAAAC ATGCCCAGGG AGCAATTTGT TACACAACCA GCCCGGTTCA TACTTTGGAC AATTTTTTGG AGCTAGGGAA AAAATTGGCT GAAATGGGAT GTGATAGTAT CGCCATAAAA GATATGGCAG GTTTGCTAAC CCCAACGGTG ACTGTCGAAT TGTATGCCGG TTTAAAACAA GCGACTGGCT TACCTGTACA TTTGCATAGT CATTCTACTT CCGGTTTGGC AAGTATATGC CATTATGAGG CCGTTTTAGC GGGTTGTAAT CACATTGATA CAGCGATTTC ATCTTTTTCT GGCGGTGCAT CTCATCCACC TACGGAAGCA TTGGTTGCCG CATTGACTGA CACTCCGTAT GACACGGAAT TGGATTTAAA TATTTTATTG GAAATAGACG ATTATTTTAA AGCTGTCCGC AAGAAGTATT CTCAATTTGA AAGTGAGGCA CAGAATATTG ACCCTCGTGT TCAATTGTAT CAAGTGCCAG GTGGCATGAT CTCAAATCTG TATAATCAGT TAAGGGAGCA GAATGCATTA GACAAGATGG ATGCTGTTCA CAAGGAAATC CCCCGCGTTC GTAAGGACTT GGGTTATCCG CCTTTGGTTA CGCCAACATC TCAAGTCGTT GGAACTCAGG CAGTTATTAA TGTTCTAACG GGAGAACGGT ATAAAACAAT AACCAATGAA GTGAAACTCT ACTGTCAGGG AAAATATGGC ACTCCTCCTG GCAAAATTAG TTCTGCTTTA CGGAAAAAAG CGATCGGACG AACAGAAGTG ATTGAAGTCA GGCCTGGCGA TTTGTTACCT AATGAATTAG ATCAATTGCA AAATGAAATA AGTGATTTGG CACTAAGTGA TGAGGATGTT CTTCTTTATG CGATGTTTCC CGAAATTGGG CGCCAATTCT TAGAGCAGCG TAAAAATAAT CAATTAATTC CTGAGCCATT ATTGACTCAA TCAAGTGCAC CAGATAATTC CGTTATGTCT GAGTTTGATA TTATTCTTCA TGGTGAGAGT TATCATGTAA AAGTAGCTGG TTATGGAATG ATTGAGCATG GTCAACAATC CTGTTTTTTG TGGGTTGATG GTGTTCCTGA AGAAGTTGTT GTGCAACATT CTGAACTTCA TGACAAAATA GAGCGTTCAT CTGTTAATAA CAAGATAGGA CCTGGAGATA TTACTGTAGC TATCCCCGGT TCTATTATTG CAATTCATGT TTCTGTTGGT GATGAAGTCA AGGCCGGGCA GGCTGTACTG GTTATTGAGG CAATGAAAAT GGAGACAGAA ATAAAAGCAC CTGCAAATGG GGTGGTTGCA GAAATATTAT GTCAAAAGGG AGACAAAGTG ACTCCAGGGC AAGTATTAAT CAGAGTGGAG GTGAGT
Details for LepnA.18123.a.B1.GE41644
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 67kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS QEELMSKTFI TDVTLRDAHQ CLIATRMRTE DMLPICNKMD DVGFWAMEVW GGATFDACLR FLKEDPWSRL RQLRQMLPNT QLSMLLRGQN LLGYRNYADD VVRAFVKLAV NNGVDVFRVF DALNDARNLK VAIDAIKSHK KHAQGAICYT TSPVHTLDNF LELGKKLAEM GCDSIAIKDM AGLLTPTVTV ELYAGLKQAT GLPVHLHSHS TSGLASICHY EAVLAGCNHI DTAISSFSGG ASHPPTEALV AALTDTPYDT ELDLNILLEI DDYFKAVRKK YSQFESEAQN IDPRVQLYQV PGGMISNLYN QLREQNALDK MDAVHKEIPR VRKDLGYPPL VTPTSQVVGT QAVINVLTGE RYKTITNEVK LYCQGKYGTP PGKISSALRK KAIGRTEVIE VRPGDLLPNE LDQLQNEISD LALSDEDVLL YAMFPEIGRQ FLEQRKNNQL IPEPLLTQSS APDNSVMSEF DIILHGESYH VKVAGYGMIE HGQQSCFLWV DGVPEEVVVQ HSELHDKIER SSVNNKIGPG DITVAIPGSI IAIHVSVGDE VKAGQAVLVI EAMKMETEIK APANGVVAEI LCQKGDKVTP GQVLIRVEVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagcc aggaagagct tatgtctaaa acgtttataa cagatgtcac cttgcgtgat gcccatcaat gcttaatcgc tacaagaatg cgtactgaag atatgttgcc aatctgtaat aaaatggatg atgtaggatt ttgggctatg gaagtctggg gaggcgctac ttttgatgcc tgtttgcgat ttttaaagga agatccctgg tcaagattgc gtcaacttcg ccaaatgtta ccgaatactc aactctctat gctgttaaga ggtcaaaatt tgttaggcta taggaattat gcagatgatg tagtccgagc gtttgttaaa ttggccgtga ataatggagt ggatgtattt cgtgtgtttg atgctttaaa tgacgcgcga aaccttaaag tagccataga cgcgattaaa tcacataaaa aacatgccca gggagcaatt tgttacacaa ccagcccggt tcatactttg gacaattttt tggagctagg gaaaaaattg gctgaaatgg gatgtgatag tatcgccata aaagatatgg caggtttgct aaccccaacg gtgactgtcg aattgtatgc cggtttaaaa caagcgactg gcttacctgt acatttgcat agtcattcta cttccggttt ggcaagtata tgccattatg aggccgtttt agcgggttgt aatcacattg atacagcgat ttcatctttt tctggcggtg catctcatcc acctacggaa gcattggttg ccgcattgac tgacactccg tatgacacgg aattggattt aaatatttta ttggaaatag acgattattt taaagctgtc cgcaagaagt attctcaatt tgaaagtgag gcacagaata ttgaccctcg tgttcaattg tatcaagtgc caggtggcat gatctcaaat ctgtataatc agttaaggga gcagaatgca ttagacaaga tggatgctgt tcacaaggaa atcccccgcg ttcgtaagga cttgggttat ccgcctttgg ttacgccaac atctcaagtc gttggaactc aggcagttat taatgttcta acgggagaac ggtataaaac aataaccaat gaagtgaaac tctactgtca gggaaaatat ggcactcctc ctggcaaaat tagttctgct ttacggaaaa aagcgatcgg acgaacagaa gtgattgaag tcaggcctgg cgatttgtta cctaatgaat tagatcaatt gcaaaatgaa ataagtgatt tggcactaag tgatgaggat gttcttcttt atgcgatgtt tcccgaaatt gggcgccaat tcttagagca gcgtaaaaat aatcaattaa ttcctgagcc attattgact caatcaagtg caccagataa ttccgttatg tctgagtttg atattattct tcatggtgag agttatcatg taaaagtagc tggttatgga atgattgagc atggtcaaca atcctgtttt ttgtgggttg atggtgttcc tgaagaagtt gttgtgcaac attctgaact tcatgacaaa atagagcgtt catctgttaa taacaagata ggacctggag atattactgt agctatcccc ggttctatta ttgcaattca tgtttctgtt ggtgatgaag tcaaggccgg gcaggctgta ctggttattg aggcaatgaa aatggagaca gaaataaaag cacctgcaaa tggggtggtt gcagaaatat tatgtcaaaa gggagacaaa gtgactccag ggcaagtatt aatcagagtg gaggtgagtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat