SSGCID: Target Page

LepnA.17966.a

Succinate dehydrogenase iron-sulfur protein subunit B

CENTER ID:	LepnA.17966.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.17966.a.B1.GE41642	Full length(LepnA.17966.a)		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272624.6.peg.555
UniProt:	Q5ZY42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADSRNLILS IYRYNPEVDA KPYMKDYEIE IPAKSDPMLL TLLERLKAEQ DPSITYRRSC REGVCGSDGM NINGTNGLAC ITHISQLKTD KIVIRPLPGF PVIRDLAVDM TQFYQQYERI EPYLQNDEVA PARERLQSPE ERAQLDGLYE CILCACCTSS CPSYWWNPDK FVGPAGLLQA RRFLADSRDK ATQHRLDLLQ DPFSVFRCRT IMNCTNVCPK GLNPTQAIAK IRTQMLTQET

NT Sequence

ATGGCTGATA GTAGAAATTT AATCCTGTCG ATTTATCGCT ATAATCCTGA GGTGGATGCC AAGCCTTACA TGAAAGATTA TGAGATAGAA ATCCCGGCTA AAAGCGATCC CATGTTACTT ACTTTGCTTG AGCGCTTGAA AGCTGAGCAA GATCCTTCTA TTACTTACAG GCGGTCATGT CGAGAAGGAG TATGTGGCTC TGACGGGATG AATATCAATG GGACTAATGG ATTAGCTTGC ATTACACATA TTTCACAATT AAAAACAGAT AAAATTGTCA TACGTCCTTT ACCAGGTTTT CCAGTCATTC GTGATTTGGC AGTTGATATG ACACAATTTT ATCAGCAATA TGAGCGCATT GAGCCTTATT TACAGAATGA TGAAGTAGCG CCTGCTCGAG AGAGGTTACA ATCTCCTGAA GAGCGAGCAC AATTGGATGG CTTGTATGAG TGCATTTTAT GCGCTTGTTG TACCAGTTCA TGTCCTTCTT ACTGGTGGAA TCCTGATAAA TTTGTAGGCC CTGCTGGACT TTTACAAGCA AGGCGTTTTT TAGCAGACAG TCGTGATAAA GCAACGCAAC ATCGTTTGGA TTTATTACAA GATCCTTTCA GTGTATTTCG TTGTCGTACT ATTATGAATT GCACGAATGT ATGCCCGAAG GGACTCAATC CGACGCAAGC GATTGCAAAA ATTCGCACGC AAATGTTGAC GCAGGAAACG

Details for LepnA.17966.a.B1.GE41642

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMA DSRNLILSIY RYNPEVDAKP YMKDYEIEIP AKSDPMLLTL LERLKAEQDP SITYRRSCRE GVCGSDGMNI NGTNGLACIT HISQLKTDKI VIRPLPGFPV IRDLAVDMTQ FYQQYERIEP YLQNDEVAPA RERLQSPEER AQLDGLYECI LCACCTSSCP SYWWNPDKFV GPAGLLQARR FLADSRDKAT QHRLDLLQDP FSVFRCRTIM NCTNVCPKGL NPTQAIAKIR XXXFDAGNVS E

Validated NT Sequence

atcttactca cttacgtttc ctgcgtcaaa cnnnnnnntn cgaatttttg caatcgcttg cgtcggattg agtcccttcg ggcatacatt cgtgcaattc ataatagtac gacaacgaaa tacactgaaa ggatcttgta ataaatccaa acgatgttgc gttgctttat cacgactgtc tgctaaaaaa cgccttgctt gtaaaagtcc agcagggcct acaaatttat caggattcca ccagtaagaa ggacatgaac tggtacaaca agcgcataaa atgcactcat acaagccatc caattgtgct cgctcttcag gagattgtaa cctctctcga gcaggcgcta cttcatcatt ctgtaaataa ggctcaatgc gctcatattg ctgataaaat tgtgtcatat caactgccaa atcacgaatg actggaaaac ctggtaaagg acgtatgaca attttatctg tttttaattg tgaaatatgt gtaatgcaag ctaatccatt agtcccattg atattcatcc cgtcagagcc acatactcct tctcgacatg accgcctgta agtaatagaa ggatcttgct cagctttcaa gcgctcaagc aaagtaagta acatgggatc gcttttagcc gggatttcta tctcataatc tttcatgtaa ggcttggcat ccacctcagg attatagcga taaatcgaca ggattaaatt tctactatca gccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg atagtagaaa tttaatcctg tcgatttatc gctataatcc tgaggtggat gccaagcctt acatgaaaga ttatgagata gaaatcccgg ctaaaagcga tcccatgtta cttactttgc ttgagcgctt gaaagctgag caagatcctt ctattactta caggcggtca tgtcgagaag gagtatgtgg ctctgacggg atgaatatca atgggactaa tggattagct tgcattacac atatttcaca attaaaaaca gataaaattg tcatacgtcc tttaccaggt tttccagtca ttcgtgattt ggcagttgat atgacacaat tttatcagca atatgagcgc attgagcctt atttacagaa tgatgaagta gcgcctgctc gagagaggtt acaatctcct gaagagcgag cacaattgga tggcttgtat gagtgcattt tatgcgcttg ttgtaccagt tcatgtcctt cttactggtg gaatcctgat aaatttgtag gccctgctgg acttttacaa gcaaggcgtt ttttagcaga cagtcgtgat aaagcaacgc aacatcgttt ggatttatta caagatcctt tcagtgtatt tcgttgtcgt actattatga attgcacgaa tgtatgcccg aagggactca atccgacgca agcgattgca aaaattcgca cgcaaatgtt gacgcaggaa acgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat