LepnA.17292.a

Type II secretory pathway protein E

CENTER ID: LepnA.17292.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.17292.a.B1.GE41635 Full length(LepnA.17292.a) 1 494
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.17292.a.B1.PW38406 Full length(LepnA.17292.a) 1 494

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.1387
UniProt: Q5ZVW9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENEEKSLKI PYSFAKSNGI VVGEIQENLA IVYHLPNTPL QAFAEIKRLL QCELDLKQVD ESTFQQHLAN IYQSKSSILD AAEGMEEDMD LSMLASQLPV SEDLLENQDD APIIRLLNAL FTQAIKQKAS DIHIETYEDR VLVRNRIDGV LQEVLEIQRA IAPLVISRVK VMAKLDIAEK RIPQDGRISL RIGGHNIDVR VSTLPSNHGE RVVLRILDKQ AAQLDLNLLG MPEHTLKTVR QMIAEPHGII LVTGPTGSGK TTSLYAMLTE LNQVTRNILT IEDPIEYDLP GVGQTQVNTK VQMTFAKGLR AILRQDPDVV MIGEIRDLET AEIAVQASLT GHLVLSTLHT NTALGALTRL HDMGVESFLL SSSIVGLIAQ RLVRKLCSHC KTPHELRQEE KELMGLKPDA DVSQVFEPKG CDYCNHLGYK GRTGIYELII VDETLRGMIH RNENIQTMEN YLRPTTPSIR EDGFKRVLCG DTSLAEILRV TSQN
NT Sequence
ATGGAAAATG AAGAAAAATC ACTTAAAATT CCTTACAGTT TTGCCAAAAG CAATGGAATT GTTGTGGGAG AAATACAAGA AAATCTGGCT ATTGTCTATC ATTTACCTAA CACACCACTG CAGGCTTTCG CTGAGATAAA AAGATTATTG CAATGCGAAC TTGATCTAAA ACAAGTCGAT GAATCCACTT TTCAGCAACA TTTAGCCAAT ATTTACCAAT CTAAATCTTC AATACTGGAT GCCGCAGAAG GCATGGAAGA AGATATGGAT TTATCCATGT TGGCCAGTCA ACTTCCAGTC AGTGAAGACT TATTGGAAAA CCAAGACGAT GCCCCAATTA TCCGCTTACT AAACGCTCTG TTTACCCAAG CGATTAAACA AAAAGCATCT GATATACACA TTGAAACTTA TGAAGACAGA GTATTGGTGC GCAATCGAAT TGATGGTGTT CTGCAAGAGG TCCTTGAAAT TCAACGAGCT ATCGCTCCTT TGGTCATTTC CAGAGTTAAG GTGATGGCCA AATTGGATAT CGCCGAAAAG AGAATTCCTC AAGATGGTCG AATTTCATTG CGTATAGGAG GACACAATAT TGATGTCAGG GTCTCAACTT TACCCTCAAA CCATGGAGAG CGAGTGGTTT TAAGGATATT GGATAAACAA GCTGCTCAAC TGGATTTAAA TCTTTTGGGT ATGCCTGAAC ATACATTAAA AACAGTACGA CAAATGATTG CTGAACCTCA TGGCATTATT TTAGTCACTG GCCCAACAGG TTCAGGCAAG ACAACATCTC TCTATGCTAT GCTCACCGAA CTAAATCAGG TGACTCGTAA TATTTTAACC ATTGAAGATC CCATTGAATA TGACCTGCCT GGTGTAGGAC AAACGCAGGT TAATACTAAA GTTCAAATGA CTTTTGCCAA AGGATTAAGG GCTATATTAC GTCAAGATCC GGATGTTGTC ATGATAGGAG AAATCCGTGA TTTGGAAACG GCTGAAATTG CTGTTCAAGC CAGCTTAACA GGGCATTTGG TACTATCAAC CCTTCATACC AATACCGCAT TGGGAGCATT AACTCGCCTG CATGATATGG GAGTTGAGTC TTTTTTATTG TCATCCAGTA TAGTCGGCCT CATTGCACAA CGTTTGGTAA GGAAATTATG CTCTCATTGC AAAACACCCC ATGAATTAAG ACAGGAAGAA AAGGAGTTAA TGGGCCTTAA ACCTGATGCT GATGTATCTC AGGTCTTTGA ACCCAAGGGA TGCGACTATT GCAACCACTT GGGTTATAAG GGAAGAACAG GAATTTATGA ATTAATTATC GTAGATGAAA CCCTGAGAGG AATGATTCAT CGTAATGAAA ATATTCAAAC GATGGAAAAT TATTTACGAC CCACCACTCC AAGCATCCGT GAAGATGGTT TTAAAAGAGT ATTATGTGGC GATACGTCAC TTGCAGAAAT TTTAAGAGTA ACCTCTCAAA AC
Details for LepnA.17292.a.B1.GE41635
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 56kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 88
PERCENT COVERAGE: 53
Validated AA Sequence
MSGSQLYPSN HGXXVXLXIX XKQAAQLDLN LLXMPEHTLK XXXQMIAEPX XIILVTGPTG SXKTTXXXXX LXXXXXVTRN XLTIEDPIEY DLPGVGQTXX XTKVQMTFAK GLRAILRQDP DVVMIGEIXX LETAEIAVQA SLTGHLVLST LHTNTALGAL TRLHDMGVES FLLSSSIVGL IAQRLVRKLC SHCKTPHELR QEEKELMGLK PDADVSQVFE PKGCDYCNHL GYKGRTGIYE LIIVDETLRG MIHRNENIQT MENYLRPTTP
Validated NT Sequence
cttggagtgg tgggtcgtaa ataattttcc atcgtttgaa tattttcatt acgatgaatc attcctctca gggtttcatc tacgataatt aattcataaa ttcctgttct tcccttataa cccaagtggt tgcaatagtc gcatcccttg ggttcaaaga cctgagatac atcagcatca ggtttaaggc ccattaactc cttttcttcc tgtcttaatt catggggtgt tttgcaatga gagcataatt tccttaccaa acgttgtgca atgaggccga ctatactgga tgacaataaa aaagactcaa ctcccatatc atgcaggcga gttaatgctc ccaatgcggt attggtatga agggttgata gtaccaaatg ccctgttaag ctggcttgaa cagcaatttc agccgtttcc aaannnnnga tttctcctat catgacaaca tccggatctt gacgtaatat agcccttaat cctttggcaa aagtcatttg aactttagta nnnnnntgcg tttgtcctac accaggcagg tcatattcaa tgggatcttc aatggttaan nnattacgag tcaccnnnnn nnnnnnnnnn agcannnnnn nnnnngntgt tgtcttnnnn gaacctgttg ggccagtgac taaaataatg cnnnnnggtt cagcaatcat ttgtcnnnnn ntttttaatg tatgttcagg catnnccaaa agatttaaat ccagttgagc agcttgtttn nnnaatatnn ttaaannnac nnnnnntcca tggtttgagg ggtaaagttg agaccctgac atcaatattg tnnnctccta tacgcaatga aattcnnnca tcttgaggan ttctcttttc ggcgatatcc aatnnngcca tcaccttaac tctggaaatg accaaaggag cgatagctcg ttgaatttca aggacctctt gcagaacacc atcaattcga ttgcgcacca atactctgtc ttcataagtt tcaatgtgta tatcagatgc tttttgttta atcgcttggg taaacagagc gtttagtaag cggataattg gggcatcgtc ttggttttcc aataagtctt cactgactgg aagttgactg gccaacatgg ataaatccat atcttcttcc atgccttctg cggcatccag tattgaagat ttagattggt aaatattggc taaatgttgc tgaaaagtgg attcatcgac ttgttttaga tcaagttcgc attgcaataa tctttttatc tcagcgaaag cctgcagtgg tgtgttaggt aaatgataga caatagccag attttcttgt atttctccca caacaattcc attgcttttg gcaaaactgt aaggaatttt aagtgatttt tct
Expected Protein Sequence
MAHHHHHHME NEEKSLKIPY SFAKSNGIVV GEIQENLAIV YHLPNTPLQA FAEIKRLLQC ELDLKQVDES TFQQHLANIY QSKSSILDAA EGMEEDMDLS MLASQLPVSE DLLENQDDAP IIRLLNALFT QAIKQKASDI HIETYEDRVL VRNRIDGVLQ EVLEIQRAIA PLVISRVKVM AKLDIAEKRI PQDGRISLRI GGHNIDVRVS TLPSNHGERV VLRILDKQAA QLDLNLLGMP EHTLKTVRQM IAEPHGIILV TGPTGSGKTT SLYAMLTELN QVTRNILTIE DPIEYDLPGV GQTQVNTKVQ MTFAKGLRAI LRQDPDVVMI GEIRDLETAE IAVQASLTGH LVLSTLHTNT ALGALTRLHD MGVESFLLSS SIVGLIAQRL VRKLCSHCKT PHELRQEEKE LMGLKPDADV SQVFEPKGCD YCNHLGYKGR TGIYELIIVD ETLRGMIHRN ENIQTMENYL RPTTPSIRED GFKRVLCGDT SLAEILRVTS QN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa atgaagaaaa atcacttaaa attccttaca gttttgccaa aagcaatgga attgttgtgg gagaaataca agaaaatctg gctattgtct atcatttacc taacacacca ctgcaggctt tcgctgagat aaaaagatta ttgcaatgcg aacttgatct aaaacaagtc gatgaatcca cttttcagca acatttagcc aatatttacc aatctaaatc ttcaatactg gatgccgcag aaggcatgga agaagatatg gatttatcca tgttggccag tcaacttcca gtcagtgaag acttattgga aaaccaagac gatgccccaa ttatccgctt actaaacgct ctgtttaccc aagcgattaa acaaaaagca tctgatatac acattgaaac ttatgaagac agagtattgg tgcgcaatcg aattgatggt gttctgcaag aggtccttga aattcaacga gctatcgctc ctttggtcat ttccagagtt aaggtgatgg ccaaattgga tatcgccgaa aagagaattc ctcaagatgg tcgaatttca ttgcgtatag gaggacacaa tattgatgtc agggtctcaa ctttaccctc aaaccatgga gagcgagtgg ttttaaggat attggataaa caagctgctc aactggattt aaatcttttg ggtatgcctg aacatacatt aaaaacagta cgacaaatga ttgctgaacc tcatggcatt attttagtca ctggcccaac aggttcaggc aagacaacat ctctctatgc tatgctcacc gaactaaatc aggtgactcg taatatttta accattgaag atcccattga atatgacctg cctggtgtag gacaaacgca ggttaatact aaagttcaaa tgacttttgc caaaggatta agggctatat tacgtcaaga tccggatgtt gtcatgatag gagaaatccg tgatttggaa acggctgaaa ttgctgttca agccagctta acagggcatt tggtactatc aacccttcat accaataccg cattgggagc attaactcgc ctgcatgata tgggagttga gtctttttta ttgtcatcca gtatagtcgg cctcattgca caacgtttgg taaggaaatt atgctctcat tgcaaaacac cccatgaatt aagacaggaa gaaaaggagt taatgggcct taaacctgat gctgatgtat ctcaggtctt tgaacccaag ggatgcgact attgcaacca cttgggttat aagggaagaa caggaattta tgaattaatt atcgtagatg aaaccctgag aggaatgatt catcgtaatg aaaatattca aacgatggaa aattatttac gacccaccac tccaagcatc cgtgaagatg gttttaaaag agtattatgt ggcgatacgt cacttgcaga aattttaaga gtaacctctc aaaactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LepnA.17292.a.B1.PW38406
PURIFICATION DATe: 12/11/2017
CONCENTRATION: 30.19mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 88
PERCENT COVERAGE: 53
Protocol Notes
notes unavailable
Validated AA Sequence
MSGSQLYPSN HGXXVXLXIX XKQAAQLDLN LLXMPEHTLK XXXQMIAEPX XIILVTGPTG SXKTTXXXXX LXXXXXVTRN XLTIEDPIEY DLPGVGQTXX XTKVQMTFAK GLRAILRQDP DVVMIGEIXX LETAEIAVQA SLTGHLVLST LHTNTALGAL TRLHDMGVES FLLSSSIVGL IAQRLVRKLC SHCKTPHELR QEEKELMGLK PDADVSQVFE PKGCDYCNHL GYKGRTGIYE LIIVDETLRG MIHRNENIQT MENYLRPTTP
Validated NT Sequence
cttggagtgg tgggtcgtaa ataattttcc atcgtttgaa tattttcatt acgatgaatc attcctctca gggtttcatc tacgataatt aattcataaa ttcctgttct tcccttataa cccaagtggt tgcaatagtc gcatcccttg ggttcaaaga cctgagatac atcagcatca ggtttaaggc ccattaactc cttttcttcc tgtcttaatt catggggtgt tttgcaatga gagcataatt tccttaccaa acgttgtgca atgaggccga ctatactgga tgacaataaa aaagactcaa ctcccatatc atgcaggcga gttaatgctc ccaatgcggt attggtatga agggttgata gtaccaaatg ccctgttaag ctggcttgaa cagcaatttc agccgtttcc aaannnnnga tttctcctat catgacaaca tccggatctt gacgtaatat agcccttaat cctttggcaa aagtcatttg aactttagta nnnnnntgcg tttgtcctac accaggcagg tcatattcaa tgggatcttc aatggttaan nnattacgag tcaccnnnnn nnnnnnnnnn agcannnnnn nnnnngntgt tgtcttnnnn gaacctgttg ggccagtgac taaaataatg cnnnnnggtt cagcaatcat ttgtcnnnnn ntttttaatg tatgttcagg catnnccaaa agatttaaat ccagttgagc agcttgtttn nnnaatatnn ttaaannnac nnnnnntcca tggtttgagg ggtaaagttg agaccctgac atcaatattg tnnnctccta tacgcaatga aattcnnnca tcttgaggan ttctcttttc ggcgatatcc aatnnngcca tcaccttaac tctggaaatg accaaaggag cgatagctcg ttgaatttca aggacctctt gcagaacacc atcaattcga ttgcgcacca atactctgtc ttcataagtt tcaatgtgta tatcagatgc tttttgttta atcgcttggg taaacagagc gtttagtaag cggataattg gggcatcgtc ttggttttcc aataagtctt cactgactgg aagttgactg gccaacatgg ataaatccat atcttcttcc atgccttctg cggcatccag tattgaagat ttagattggt aaatattggc taaatgttgc tgaaaagtgg attcatcgac ttgttttaga tcaagttcgc attgcaataa tctttttatc tcagcgaaag cctgcagtgg tgtgttaggt aaatgataga caatagccag attttcttgt atttctccca caacaattcc attgcttttg gcaaaactgt aaggaatttt aagtgatttt tct
Expressed Protein Sequence
MAHHHHHHME NEEKSLKIPY SFAKSNGIVV GEIQENLAIV YHLPNTPLQA FAEIKRLLQC ELDLKQVDES TFQQHLANIY QSKSSILDAA EGMEEDMDLS MLASQLPVSE DLLENQDDAP IIRLLNALFT QAIKQKASDI HIETYEDRVL VRNRIDGVLQ EVLEIQRAIA PLVISRVKVM AKLDIAEKRI PQDGRISLRI GGHNIDVRVS TLPSNHGERV VLRILDKQAA QLDLNLLGMP EHTLKTVRQM IAEPHGIILV TGPTGSGKTT SLYAMLTELN QVTRNILTIE DPIEYDLPGV GQTQVNTKVQ MTFAKGLRAI LRQDPDVVMI GEIRDLETAE IAVQASLTGH LVLSTLHTNT ALGALTRLHD MGVESFLLSS SIVGLIAQRL VRKLCSHCKT PHELRQEEKE LMGLKPDADV SQVFEPKGCD YCNHLGYKGR TGIYELIIVD ETLRGMIHRN ENIQTMENYL RPTTPSIRED GFKRVLCGDT SLAEILRVTS QN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA ATGAAGAAAA ATCACTTAAA ATTCCTTACA GTTTTGCCAA AAGCAATGGA ATTGTTGTGG GAGAAATACA AGAAAATCTG GCTATTGTCT ATCATTTACC TAACACACCA CTGCAGGCTT TCGCTGAGAT AAAAAGATTA TTGCAATGCG AACTTGATCT AAAACAAGTC GATGAATCCA CTTTTCAGCA ACATTTAGCC AATATTTACC AATCTAAATC TTCAATACTG GATGCCGCAG AAGGCATGGA AGAAGATATG GATTTATCCA TGTTGGCCAG TCAACTTCCA GTCAGTGAAG ACTTATTGGA AAACCAAGAC GATGCCCCAA TTATCCGCTT ACTAAACGCT CTGTTTACCC AAGCGATTAA ACAAAAAGCA TCTGATATAC ACATTGAAAC TTATGAAGAC AGAGTATTGG TGCGCAATCG AATTGATGGT GTTCTGCAAG AGGTCCTTGA AATTCAACGA GCTATCGCTC CTTTGGTCAT TTCCAGAGTT AAGGTGATGG CCAAATTGGA TATCGCCGAA AAGAGAATTC CTCAAGATGG TCGAATTTCA TTGCGTATAG GAGGACACAA TATTGATGTC AGGGTCTCAA CTTTACCCTC AAACCATGGA GAGCGAGTGG TTTTAAGGAT ATTGGATAAA CAAGCTGCTC AACTGGATTT AAATCTTTTG GGTATGCCTG AACATACATT AAAAACAGTA CGACAAATGA TTGCTGAACC TCATGGCATT ATTTTAGTCA CTGGCCCAAC AGGTTCAGGC AAGACAACAT CTCTCTATGC TATGCTCACC GAACTAAATC AGGTGACTCG TAATATTTTA ACCATTGAAG ATCCCATTGA ATATGACCTG CCTGGTGTAG GACAAACGCA GGTTAATACT AAAGTTCAAA TGACTTTTGC CAAAGGATTA AGGGCTATAT TACGTCAAGA TCCGGATGTT GTCATGATAG GAGAAATCCG TGATTTGGAA ACGGCTGAAA TTGCTGTTCA AGCCAGCTTA ACAGGGCATT TGGTACTATC AACCCTTCAT ACCAATACCG CATTGGGAGC ATTAACTCGC CTGCATGATA TGGGAGTTGA GTCTTTTTTA TTGTCATCCA GTATAGTCGG CCTCATTGCA CAACGTTTGG TAAGGAAATT ATGCTCTCAT TGCAAAACAC CCCATGAATT AAGACAGGAA GAAAAGGAGT TAATGGGCCT TAAACCTGAT GCTGATGTAT CTCAGGTCTT TGAACCCAAG GGATGCGACT ATTGCAACCA CTTGGGTTAT AAGGGAAGAA CAGGAATTTA TGAATTAATT ATCGTAGATG AAACCCTGAG AGGAATGATT CATCGTAATG AAAATATTCA AACGATGGAA AATTATTTAC GACCCACCAC TCCAAGCATC CGTGAAGATG GTTTTAAAAG AGTATTATGT GGCGATACGT CACTTGCAGA AATTTTAAGA GTAACCTCTC AAAACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT