LepnA.10201.a

Methionine--tRNA ligase

CENTER ID: LepnA.10201.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.10201.a.B1.GE41634 Full length(LepnA.10201.a) 1 670
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.10201.a.B1.PW38391 Full length(LepnA.10201.a) 1 670

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.3070
UniProt: Q5ZRJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSERKMLVT SALPYANGHL HLGHLVEHIQ TDIWVRTHKM LGIQCISVCG DDAHGTPIML KAEQLGITPE ALTAEIKLSH EKDFKAFAID YDYYHTTHSP ENQALATTIF ERLQAGGDIV KKTIRQFYDP VKQMFLPDRY VKGTCPKCAA VDQYGDNCEV CGATYSPTDL INPVSVISGV SPVEKESEHY FFDLPRYEEL LKDWTRKGHL QTEVTNKLSE WFEAGLKQWD ISRDAPYFGF PIPGVPDKYF YVWLDAPIGY MASFKKYCDE RGVSFDEFWD KASKTELYHF VGKDIVYFHA LFWPAMLAAS GFRTPTAVYT HGFLTVEGQK MSKSRGTFIE ARAYLAHLHP EYLRYYFAAK LNGRVDDLDL NFDDFVNRVN ADLVGKVVNI ASRCAGFINK RFDNRLSSEL NDQKLYNDLL SARESVIDAF VSRDYARAIR QIMDCADKVN QYIDANKPWV LAKDESKLNE VHAICTMGIN LFRILITYLK PVLPMMAKAS EEFLNSEPLH WDSIDKPLLN HRINTFKPLM VRVEKEKIEA MLVQSKESLM TTPVKENTPV EDANLISIED FAKVDLRIAK IVNAEPVEGA DKLMRLILDL GDAQKQVFAG IKKAYDAEEL IGRLTVMVAN LEPRTMRFGV SEGMVLAAGD GQGIYLLQPD AGALPGMKVK
NT Sequence
ATGACTAGTG AACGAAAAAT GTTAGTGACC AGTGCACTGC CTTATGCTAA TGGGCATTTG CATCTGGGAC ATTTGGTTGA GCATATTCAA ACAGATATTT GGGTCCGTAC TCACAAAATG TTGGGTATTC AATGTATTAG CGTTTGTGGG GATGACGCTC ATGGAACACC CATTATGCTG AAAGCAGAGC AACTGGGAAT TACTCCGGAA GCTTTAACCG CCGAAATTAA GTTAAGTCAT GAAAAGGATT TTAAAGCCTT CGCCATTGAT TATGATTATT ACCATACCAC CCATTCACCT GAAAATCAGG CATTGGCGAC TACCATATTT GAACGTTTGC AGGCAGGCGG TGATATAGTC AAGAAAACAA TTCGCCAGTT TTATGATCCC GTAAAGCAAA TGTTTTTGCC GGACAGGTAT GTGAAGGGAA CTTGCCCCAA ATGTGCTGCT GTCGATCAGT ATGGCGATAA TTGCGAGGTT TGTGGCGCCA CTTATTCGCC GACGGATTTG ATTAACCCTG TTTCTGTTAT ATCGGGAGTC AGCCCAGTAG AAAAAGAATC AGAGCATTAT TTTTTTGATT TGCCGCGTTA CGAAGAGTTA CTTAAGGATT GGACAAGAAA GGGGCATTTA CAAACCGAAG TAACGAATAA ACTCAGCGAA TGGTTTGAGG CGGGTTTAAA GCAATGGGAT ATTTCTCGCG ATGCTCCCTA TTTTGGCTTC CCTATCCCCG GTGTTCCGGA TAAATACTTT TACGTTTGGT TAGATGCTCC CATAGGCTAT ATGGCAAGCT TTAAAAAGTA TTGCGATGAG CGTGGCGTTT CTTTTGATGA GTTTTGGGAT AAGGCTTCAA AAACAGAATT ATATCATTTT GTAGGCAAGG ATATTGTGTA TTTTCATGCT TTGTTCTGGC CAGCTATGTT AGCTGCCAGC GGTTTCAGAA CACCTACGGC CGTGTATACC CATGGGTTTT TAACGGTTGA GGGGCAGAAA ATGTCCAAAT CGCGCGGTAC TTTTATAGAA GCACGCGCTT ATCTGGCTCA CTTGCATCCT GAATATCTGC GTTACTATTT CGCTGCCAAA TTGAATGGTC GAGTAGACGA TTTGGATTTG AATTTTGACG ATTTTGTCAA TAGGGTGAAT GCGGATCTGG TCGGGAAGGT AGTGAATATT GCAAGCCGAT GTGCGGGATT TATTAATAAG CGTTTTGATA ATCGGTTGAG CTCAGAGTTG AATGATCAAA AATTATACAA CGACTTGCTG AGCGCAAGAG AGTCCGTGAT TGACGCATTT GTTTCAAGAG ACTATGCGCG GGCAATAAGA CAAATCATGG ATTGTGCTGA CAAAGTCAAT CAATACATCG ATGCCAATAA ACCTTGGGTA TTGGCTAAAG ATGAAAGCAA ATTAAATGAA GTTCATGCCA TATGCACAAT GGGAATCAAT TTATTCCGCA TTTTAATAAC CTATTTAAAA CCAGTCTTGC CAATGATGGC CAAAGCTTCA GAAGAATTTT TAAATTCTGA GCCTTTACAC TGGGATTCTA TCGATAAACC TTTGCTAAAC CATCGTATCA ATACTTTTAA GCCTTTGATG GTTAGAGTAG AAAAAGAAAA AATTGAAGCG ATGTTGGTTC AAAGCAAGGA ATCTCTGATG ACTACCCCAG TAAAAGAAAA CACGCCAGTT GAGGACGCTA ACTTAATCAG TATTGAGGAC TTTGCCAAAG TCGATTTACG GATTGCAAAA ATTGTCAATG CAGAGCCTGT GGAAGGCGCA GATAAACTAA TGCGCTTGAT TTTAGACTTG GGTGATGCTC AAAAGCAGGT TTTTGCCGGC ATAAAAAAGG CTTATGATGC TGAAGAATTG ATTGGGCGTT TGACAGTAAT GGTTGCTAAT TTGGAGCCCC GCACTATGCG TTTCGGTGTT TCTGAAGGCA TGGTATTGGC GGCAGGGGAT GGGCAGGGAA TTTATTTATT ACAGCCTGAT GCAGGAGCAC TTCCTGGCAT GAAGGTGAAG
Details for LepnA.10201.a.B1.GE41634
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 77kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT SERKMLVTSA LPYANGHLHL GHLVEHIQTD IWVRTHKMLG IQCISVCGDD AHGTPIMLKA EQLGITPEAL TAEIKLSHEK DFKAFAIDYD YYHTTHSPEN QALATTIFER LQAGGDIVKK TIRQFYDPVK QMFLPDRYVK GTCPKCAAVD QYGDNCEVCG ATYSPTDLIN PVSVISGVSP VEKESEHYFF DLPRYEELLK DWTRKGHLQT EVTNKLSEWF EAGLKQWDIS RDAPYFGFPI PGVPDKYFYV WLDAPIGYMA SFKKYCDERG VSFDEFWDKA SKTELYHFVG KDIVYFHALF WPAMLAASGF RTPTAVYTHG FLTVEGQKMS KSRGTFIEAR AYLAHLHPEY LRYYFAAKLN GRVDDLDLNF DDFVNRVNAD LVGKVVNIAS RCAGFINKRF DNRLSSELND QKLYNDLLSA RESVIDAFVS RDYARAIRQI MDCADKVNQY IDANKPWVLA KDESKLNEVH AICTMGINLF RILITYLKPV LPMMAKASEE FLNSEPLHWD SIDKPLLNHR INTFKPLMVR VEKEKIEAML VQSKESLMTT PVKENTPVED ANLISIEDFA KVDLRIAKIV NAEPVEGADK LMRLILDLGD AQKQVFAGIK KAYDAEELIG RLTVMVANLE PRTMRFGVSE GMVLAAGDGQ GIYLLQPDAG ALPGMKVK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta gtgaacgaaa aatgttagtg accagtgcac tgccttatgc taatgggcat ttgcatctgg gacatttggt tgagcatatt caaacagata tttgggtccg tactcacaaa atgttgggta ttcaatgtat tagcgtttgt ggggatgacg ctcatggaac acccattatg ctgaaagcag agcaactggg aattactccg gaagctttaa ccgccgaaat taagttaagt catgaaaagg attttaaagc cttcgccatt gattatgatt attaccatac cacccattca cctgaaaatc aggcattggc gactaccata tttgaacgtt tgcaggcagg cggtgatata gtcaagaaaa caattcgcca gttttatgat cccgtaaagc aaatgttttt gccggacagg tatgtgaagg gaacttgccc caaatgtgct gctgtcgatc agtatggcga taattgcgag gtttgtggcg ccacttattc gccgacggat ttgattaacc ctgtttctgt tatatcggga gtcagcccag tagaaaaaga atcagagcat tatttttttg atttgccgcg ttacgaagag ttacttaagg attggacaag aaaggggcat ttacaaaccg aagtaacgaa taaactcagc gaatggtttg aggcgggttt aaagcaatgg gatatttctc gcgatgctcc ctattttggc ttccctatcc ccggtgttcc ggataaatac ttttacgttt ggttagatgc tcccataggc tatatggcaa gctttaaaaa gtattgcgat gagcgtggcg tttcttttga tgagttttgg gataaggctt caaaaacaga attatatcat tttgtaggca aggatattgt gtattttcat gctttgttct ggccagctat gttagctgcc agcggtttca gaacacctac ggccgtgtat acccatgggt ttttaacggt tgaggggcag aaaatgtcca aatcgcgcgg tacttttata gaagcacgcg cttatctggc tcacttgcat cctgaatatc tgcgttacta tttcgctgcc aaattgaatg gtcgagtaga cgatttggat ttgaattttg acgattttgt caatagggtg aatgcggatc tggtcgggaa ggtagtgaat attgcaagcc gatgtgcggg atttattaat aagcgttttg ataatcggtt gagctcagag ttgaatgatc aaaaattata caacgacttg ctgagcgcaa gagagtccgt gattgacgca tttgtttcaa gagactatgc gcgggcaata agacaaatca tggattgtgc tgacaaagtc aatcaataca tcgatgccaa taaaccttgg gtattggcta aagatgaaag caaattaaat gaagttcatg ccatatgcac aatgggaatc aatttattcc gcattttaat aacctattta aaaccagtct tgccaatgat ggccaaagct tcagaagaat ttttaaattc tgagccttta cactgggatt ctatcgataa acctttgcta aaccatcgta tcaatacttt taagcctttg atggttagag tagaaaaaga aaaaattgaa gcgatgttgg ttcaaagcaa ggaatctctg atgactaccc cagtaaaaga aaacacgcca gttgaggacg ctaacttaat cagtattgag gactttgcca aagtcgattt acggattgca aaaattgtca atgcagagcc tgtggaaggc gcagataaac taatgcgctt gattttagac ttgggtgatg ctcaaaagca ggtttttgcc ggcataaaaa aggcttatga tgctgaagaa ttgattgggc gtttgacagt aatggttgct aatttggagc cccgcactat gcgtttcggt gtttctgaag gcatggtatt ggcggcaggg gatgggcagg gaatttattt attacagcct gatgcaggag cacttcctgg catgaaggtg aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LepnA.10201.a.B1.PW38391
PURIFICATION DATe: 11/15/2017
CONCENTRATION: 28.23mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMT SERKMLVTSA LPYANGHLHL GHLVEHIQTD IWVRTHKMLG IQCISVCGDD AHGTPIMLKA EQLGITPEAL TAEIKLSHEK DFKAFAIDYD YYHTTHSPEN QALATTIFER LQAGGDIVKK TIRQFYDPVK QMFLPDRYVK GTCPKCAAVD QYGDNCEVCG ATYSPTDLIN PVSVISGVSP VEKESEHYFF DLPRYEELLK DWTRKGHLQT EVTNKLSEWF EAGLKQWDIS RDAPYFGFPI PGVPDKYFYV WLDAPIGYMA SFKKYCDERG VSFDEFWDKA SKTELYHFVG KDIVYFHALF WPAMLAASGF RTPTAVYTHG FLTVEGQKMS KSRGTFIEAR AYLAHLHPEY LRYYFAAKLN GRVDDLDLNF DDFVNRVNAD LVGKVVNIAS RCAGFINKRF DNRLSSELND QKLYNDLLSA RESVIDAFVS RDYARAIRQI MDCADKVNQY IDANKPWVLA KDESKLNEVH AICTMGINLF RILITYLKPV LPMMAKASEE FLNSEPLHWD SIDKPLLNHR INTFKPLMVR VEKEKIEAML VQSKESLMTT PVKENTPVED ANLISIEDFA KVDLRIAKIV NAEPVEGADK LMRLILDLGD AQKQVFAGIK KAYDAEELIG RLTVMVANLE PRTMRFGVSE GMVLAAGDGQ GIYLLQPDAG ALPGMKVK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTA GTGAACGAAA AATGTTAGTG ACCAGTGCAC TGCCTTATGC TAATGGGCAT TTGCATCTGG GACATTTGGT TGAGCATATT CAAACAGATA TTTGGGTCCG TACTCACAAA ATGTTGGGTA TTCAATGTAT TAGCGTTTGT GGGGATGACG CTCATGGAAC ACCCATTATG CTGAAAGCAG AGCAACTGGG AATTACTCCG GAAGCTTTAA CCGCCGAAAT TAAGTTAAGT CATGAAAAGG ATTTTAAAGC CTTCGCCATT GATTATGATT ATTACCATAC CACCCATTCA CCTGAAAATC AGGCATTGGC GACTACCATA TTTGAACGTT TGCAGGCAGG CGGTGATATA GTCAAGAAAA CAATTCGCCA GTTTTATGAT CCCGTAAAGC AAATGTTTTT GCCGGACAGG TATGTGAAGG GAACTTGCCC CAAATGTGCT GCTGTCGATC AGTATGGCGA TAATTGCGAG GTTTGTGGCG CCACTTATTC GCCGACGGAT TTGATTAACC CTGTTTCTGT TATATCGGGA GTCAGCCCAG TAGAAAAAGA ATCAGAGCAT TATTTTTTTG ATTTGCCGCG TTACGAAGAG TTACTTAAGG ATTGGACAAG AAAGGGGCAT TTACAAACCG AAGTAACGAA TAAACTCAGC GAATGGTTTG AGGCGGGTTT AAAGCAATGG GATATTTCTC GCGATGCTCC CTATTTTGGC TTCCCTATCC CCGGTGTTCC GGATAAATAC TTTTACGTTT GGTTAGATGC TCCCATAGGC TATATGGCAA GCTTTAAAAA GTATTGCGAT GAGCGTGGCG TTTCTTTTGA TGAGTTTTGG GATAAGGCTT CAAAAACAGA ATTATATCAT TTTGTAGGCA AGGATATTGT GTATTTTCAT GCTTTGTTCT GGCCAGCTAT GTTAGCTGCC AGCGGTTTCA GAACACCTAC GGCCGTGTAT ACCCATGGGT TTTTAACGGT TGAGGGGCAG AAAATGTCCA AATCGCGCGG TACTTTTATA GAAGCACGCG CTTATCTGGC TCACTTGCAT CCTGAATATC TGCGTTACTA TTTCGCTGCC AAATTGAATG GTCGAGTAGA CGATTTGGAT TTGAATTTTG ACGATTTTGT CAATAGGGTG AATGCGGATC TGGTCGGGAA GGTAGTGAAT ATTGCAAGCC GATGTGCGGG ATTTATTAAT AAGCGTTTTG ATAATCGGTT GAGCTCAGAG TTGAATGATC AAAAATTATA CAACGACTTG CTGAGCGCAA GAGAGTCCGT GATTGACGCA TTTGTTTCAA GAGACTATGC GCGGGCAATA AGACAAATCA TGGATTGTGC TGACAAAGTC AATCAATACA TCGATGCCAA TAAACCTTGG GTATTGGCTA AAGATGAAAG CAAATTAAAT GAAGTTCATG CCATATGCAC AATGGGAATC AATTTATTCC GCATTTTAAT AACCTATTTA AAACCAGTCT TGCCAATGAT GGCCAAAGCT TCAGAAGAAT TTTTAAATTC TGAGCCTTTA CACTGGGATT CTATCGATAA ACCTTTGCTA AACCATCGTA TCAATACTTT TAAGCCTTTG ATGGTTAGAG TAGAAAAAGA AAAAATTGAA GCGATGTTGG TTCAAAGCAA GGAATCTCTG ATGACTACCC CAGTAAAAGA AAACACGCCA GTTGAGGACG CTAACTTAAT CAGTATTGAG GACTTTGCCA AAGTCGATTT ACGGATTGCA AAAATTGTCA ATGCAGAGCC TGTGGAAGGC GCAGATAAAC TAATGCGCTT GATTTTAGAC TTGGGTGATG CTCAAAAGCA GGTTTTTGCC GGCATAAAAA AGGCTTATGA TGCTGAAGAA TTGATTGGGC GTTTGACAGT AATGGTTGCT AATTTGGAGC CCCGCACTAT GCGTTTCGGT GTTTCTGAAG GCATGGTATT GGCGGCAGGG GATGGGCAGG GAATTTATTT ATTACAGCCT GATGCAGGAG CACTTCCTGG CATGAAGGTG AAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT