LepnA.01267.a
Multifunctional: 2-keto-3-deoxygluconate 6-phosphate aldolase/(4-hydroxy-2-oxoglutarate aldolase)
| CENTER ID: | LepnA.01267.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01267.a.B1.GE41628 | Full length(LepnA.01267.a) | 1 | 220 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01267.a.B1.PS38520 | Full length(LepnA.01267.a) | 1 | 220 |
Structures
6OVI
DEPOSITED: 5/7/2019
DETERMINATION: XRay
CLONE: LepnA.01267.a.B1.GE41628
PROTEIN: LepnA.01267.a.B1.PS38520
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.436 |
| UniProt: | Q5ZYF2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFSEWKTQP GAVFAASPVI PVIVIKELED ALPLAEALFA GGIHVLEVTL RTPVAIKALE LLINTFPDEL IGAGTVITPG QFHDVVAAGA RFAISPGQTR ELLIAGQKSE IPLIPGVASV SELMEGLGMG YNHFKFFPAA AAGGIPMLKA ISGVFPQVKF CPTGGINSKN YEEYLCLPNV ACVGGSWIVP EEAIKNHNWS LITELCMAVS SQKRELCSQN
NT Sequence
ATGTCCTTTT CTGAATGGAA AACACAACCA GGCGCGGTAT TTGCTGCATC TCCGGTTATT CCAGTCATTG TAATCAAAGA GTTAGAGGAT GCGTTACCTT TGGCCGAGGC TTTATTTGCC GGAGGAATTC ATGTTCTTGA AGTGACATTA AGAACACCAG TGGCAATAAA AGCACTGGAA TTATTGATCA ATACGTTTCC AGATGAATTA ATCGGTGCCG GAACTGTGAT TACCCCTGGC CAGTTTCATG ATGTGGTAGC AGCAGGAGCT CGATTTGCCA TTAGCCCTGG CCAAACAAGG GAATTATTAA TAGCAGGACA GAAAAGTGAA ATTCCTCTCA TTCCAGGTGT AGCAAGTGTC TCTGAATTAA TGGAAGGTTT GGGGATGGGA TATAACCATT TTAAATTTTT TCCTGCGGCA GCGGCTGGAG GTATCCCTAT GTTGAAAGCG ATATCGGGCG TATTCCCCCA AGTTAAATTT TGTCCAACAG GCGGAATTAA TTCCAAAAAT TATGAGGAGT ATCTTTGCTT GCCAAATGTA GCTTGCGTTG GAGGGTCCTG GATTGTACCG GAAGAGGCTA TCAAGAATCA TAACTGGTCT TTAATTACAG AGTTATGTAT GGCGGTGAGT TCTCAGAAGC GAGAACTTTG CTCGCAGAAT
Details for LepnA.01267.a.B1.GE41628
| HARVESTED ON: | 6/23/2017 |
| SEQUENCED ON: | 6/28/2017 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS FSEWKTQPGA VFAASPVIPV IVIKELEDAL PLAEALFAGG IHVLEVTLRT PVAIKALELL INTFPDELIG AGTVITPGQF HDVVAAGARF AISPGQTREL LIAGQKSEIP LIPGVASVSE LMEGLGMGYN HFKFFPAAAA GGIPMLKAIS GVFPQVKFCP TGGINSKNYE EYLCLPNVAC VGGSWIVPEE AIKNHNWSLI TELCMAVSSQ XXXLCSXX
Validated NT Sequence
atcttactca cttaatnnng cgagcaaagt nnnnnnnnct gagaactcac cgccatacat aactctgtaa ttaaagacca gttatgattc ttgatagcct cttccggtac aatccaggac cctccaacgc aagctacatt tggcaagcaa agatactcct cataattttt ggaattaatt ccgcctgttg gacaaaattt aacttggggg aatacgcccg atatcgcttt caacataggg atacctccag ccgctgccgc aggaaaaaat ttaaaatggt tatatcccat ccccaaacct tccattaatt cagagacact tgctacacct ggaatgagag gaatttcact tttctgtcct gctattaata attcccttgt ttggccaggg ctaatggcaa atcgagctcc tgctgctacc acatcatgaa actggccagg ggtaatcaca gttccggcac cgattaattc atctggaaac gtattgatca ataattccag tgcttttatt gccactggtg ttcttaatgt cacttcaaga acatgaattc ctccggcaaa taaagcctcg gccaaaggta acgcatcctc taactctttg attacaatga ctggaataac cggagatgca gcaaataccg cgcctggttg tgttttccat tcagaaaagg acatatggtg gtggtggtgg tgagccatgg tatat
Expected Protein Sequence
MAHHHHHHMS FSEWKTQPGA VFAASPVIPV IVIKELEDAL PLAEALFAGG IHVLEVTLRT PVAIKALELL INTFPDELIG AGTVITPGQF HDVVAAGARF AISPGQTREL LIAGQKSEIP LIPGVASVSE LMEGLGMGYN HFKFFPAAAA GGIPMLKAIS GVFPQVKFCP TGGINSKNYE EYLCLPNVAC VGGSWIVPEE AIKNHNWSLI TELCMAVSSQ KRELCSQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct tttctgaatg gaaaacacaa ccaggcgcgg tatttgctgc atctccggtt attccagtca ttgtaatcaa agagttagag gatgcgttac ctttggccga ggctttattt gccggaggaa ttcatgttct tgaagtgaca ttaagaacac cagtggcaat aaaagcactg gaattattga tcaatacgtt tccagatgaa ttaatcggtg ccggaactgt gattacccct ggccagtttc atgatgtggt agcagcagga gctcgatttg ccattagccc tggccaaaca agggaattat taatagcagg acagaaaagt gaaattcctc tcattccagg tgtagcaagt gtctctgaat taatggaagg tttggggatg ggatataacc attttaaatt ttttcctgcg gcagcggctg gaggtatccc tatgttgaaa gcgatatcgg gcgtattccc ccaagttaaa ttttgtccaa caggcggaat taattccaaa aattatgagg agtatctttg cttgccaaat gtagcttgcg ttggagggtc ctggattgta ccggaagagg ctatcaagaa tcataactgg tctttaatta cagagttatg tatggcggtg agttctcaga agcgagaact ttgctcgcag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LepnA.01267.a.B1.PS38520
| PURIFICATION DATe: | 10/23/2018 |
| CONCENTRATION: | 36.05mg/ml |
| OBSERVED MW: | 24kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS FSEWKTQPGA VFAASPVIPV IVIKELEDAL PLAEALFAGG IHVLEVTLRT PVAIKALELL INTFPDELIG AGTVITPGQF HDVVAAGARF AISPGQTREL LIAGQKSEIP LIPGVASVSE LMEGLGMGYN HFKFFPAAAA GGIPMLKAIS GVFPQVKFCP TGGINSKNYE EYLCLPNVAC VGGSWIVPEE AIKNHNWSLI TELCMAVSSQ XXXLCSXX
Validated NT Sequence
atcttactca cttaatnnng cgagcaaagt nnnnnnnnct gagaactcac cgccatacat aactctgtaa ttaaagacca gttatgattc ttgatagcct cttccggtac aatccaggac cctccaacgc aagctacatt tggcaagcaa agatactcct cataattttt ggaattaatt ccgcctgttg gacaaaattt aacttggggg aatacgcccg atatcgcttt caacataggg atacctccag ccgctgccgc aggaaaaaat ttaaaatggt tatatcccat ccccaaacct tccattaatt cagagacact tgctacacct ggaatgagag gaatttcact tttctgtcct gctattaata attcccttgt ttggccaggg ctaatggcaa atcgagctcc tgctgctacc acatcatgaa actggccagg ggtaatcaca gttccggcac cgattaattc atctggaaac gtattgatca ataattccag tgcttttatt gccactggtg ttcttaatgt cacttcaaga acatgaattc ctccggcaaa taaagcctcg gccaaaggta acgcatcctc taactctttg attacaatga ctggaataac cggagatgca gcaaataccg cgcctggttg tgttttccat tcagaaaagg acatatggtg gtggtggtgg tgagccatgg tatat
Expressed Protein Sequence
MAHHHHHHMS FSEWKTQPGA VFAASPVIPV IVIKELEDAL PLAEALFAGG IHVLEVTLRT PVAIKALELL INTFPDELIG AGTVITPGQF HDVVAAGARF AISPGQTREL LIAGQKSEIP LIPGVASVSE LMEGLGMGYN HFKFFPAAAA GGIPMLKAIS GVFPQVKFCP TGGINSKNYE EYLCLPNVAC VGGSWIVPEE AIKNHNWSLI TELCMAVSSQ KRELCSQN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT TTTCTGAATG GAAAACACAA CCAGGCGCGG TATTTGCTGC ATCTCCGGTT ATTCCAGTCA TTGTAATCAA AGAGTTAGAG GATGCGTTAC CTTTGGCCGA GGCTTTATTT GCCGGAGGAA TTCATGTTCT TGAAGTGACA TTAAGAACAC CAGTGGCAAT AAAAGCACTG GAATTATTGA TCAATACGTT TCCAGATGAA TTAATCGGTG CCGGAACTGT GATTACCCCT GGCCAGTTTC ATGATGTGGT AGCAGCAGGA GCTCGATTTG CCATTAGCCC TGGCCAAACA AGGGAATTAT TAATAGCAGG ACAGAAAAGT GAAATTCCTC TCATTCCAGG TGTAGCAAGT GTCTCTGAAT TAATGGAAGG TTTGGGGATG GGATATAACC ATTTTAAATT TTTTCCTGCG GCAGCGGCTG GAGGTATCCC TATGTTGAAA GCGATATCGG GCGTATTCCC CCAAGTTAAA TTTTGTCCAA CAGGCGGAAT TAATTCCAAA AATTATGAGG AGTATCTTTG CTTGCCAAAT GTAGCTTGCG TTGGAGGGTC CTGGATTGTA CCGGAAGAGG CTATCAAGAA TCATAACTGG TCTTTAATTA CAGAGTTATG TATGGCGGTG AGTTCTCAGA AGCGAGAACT TTGCTCGCAG AATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT