LepnA.01212.a
Malate dehydrogenase
| CENTER ID: | LepnA.01212.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01212.a.B1.GE41627 | Full length(LepnA.01212.a) | 1 | 330 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01212.a.B1.PS38424 | Full length(LepnA.01212.a) | 1 | 330 |
Structures
6PBL
DEPOSITED: 6/14/2019
DETERMINATION: XRay
CLONE: LepnA.01212.a.B1.GE41627
PROTEIN: LepnA.01212.a.B1.PS38424
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272624.6.peg.2473 |
| UniProt: | Q5ZT13 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNNRVRVAV TGAAGQIGYA LVFRIASGQM FGPNTEVELN LLELEPALPS LEGVAMELDD CAFPLLKRIV CTADLNKAMD GVNWALLVGS VPRKQGMERS DLLQINGGIF TKQGQAINDY ASDDVRVFVV GNPCNTNCLI AMNHAKDVPS DRFYAMTTLD ELRARTQLAK KAGVDITAVT QMTIWGNHSA TQYPDFYNAK INGTSAAQVI NDETWLKETF VSTVQQRGAA VIKARGSSSA ASAANAIITG VNHLVTDTPA GESFSMCRRS KGEYGVDEGL IFSFPCRREH GELKVVENLE FNDFGRERFN TTLNELRSER DTVKSLGLLD
NT Sequence
ATGACAAATA ATCGAGTAAG AGTTGCTGTC ACAGGTGCAG CAGGGCAAAT AGGTTATGCC TTGGTGTTTC GTATTGCTTC CGGTCAAATG TTTGGGCCAA ATACTGAAGT GGAATTGAAT TTACTTGAAC TTGAGCCGGC ACTTCCCTCA CTGGAAGGAG TCGCTATGGA GCTTGATGAT TGCGCATTTC CCTTATTAAA ACGCATTGTT TGTACTGCAG ATTTGAATAA GGCAATGGAT GGTGTTAACT GGGCATTATT AGTCGGTTCA GTTCCGCGAA AGCAAGGCAT GGAGCGTTCT GATTTATTAC AAATCAATGG TGGTATATTC ACCAAACAAG GTCAGGCGAT TAATGATTAT GCCAGTGATG ATGTACGGGT ATTTGTTGTG GGAAACCCTT GCAATACCAA TTGCCTAATT GCTATGAATC ACGCAAAAGA CGTGCCTTCT GATCGTTTTT ATGCAATGAC CACATTGGAT GAGTTAAGAG CTCGTACTCA ATTGGCTAAA AAAGCAGGCG TTGACATTAC TGCAGTGACT CAAATGACGA TTTGGGGTAA TCATTCCGCC ACCCAATATC CCGATTTTTA TAACGCAAAA ATCAATGGAA CTTCTGCTGC GCAAGTCATT AATGATGAAA CCTGGTTAAA AGAGACTTTT GTATCAACCG TCCAGCAACG AGGAGCCGCA GTGATCAAGG CACGGGGTTC ATCTTCTGCT GCTTCTGCCG CCAATGCGAT AATTACAGGA GTTAATCATT TGGTTACGGA TACTCCTGCT GGCGAATCGT TTTCAATGTG CCGTCGCTCA AAGGGAGAGT ACGGAGTCGA TGAAGGTTTG ATTTTCTCTT TCCCATGCCG AAGAGAACAT GGCGAATTAA AAGTGGTCGA GAACTTGGAG TTCAACGATT TTGGCCGCGA GAGATTCAAT ACCACTTTAA ACGAATTACG AAGTGAGCGT GACACGGTCA AGTCTTTAGG CTTATTGGAT
Details for LepnA.01212.a.B1.GE41627
| HARVESTED ON: | 6/23/2017 |
| SEQUENCED ON: | 6/28/2017 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 72 |
| PERCENT COVERAGE: | 3 |
Validated AA Sequence
MAHHHHHHXX X
Validated NT Sequence
ctctcgcggc caaaatcgtt gaactccaag ttctcgacca cttttaattc gccatgttct cttcggcatg ggaaagagaa aatcaaacct tcatcgactc cgtactctcc ctttgagcga cggcacattg aaaacgattc gccagcagga gtatccgtaa ccaaatgatt aactcctgta attatcgcat tggcggcaga agcagcagaa gatgaacccc gtgccttgat cactgcggct cctcgttgct ggacggttga tacaaaagtc tcttttaacc aggtttcatc attaatgact tgcgcagcag aagttccatt gatttttgcg ttataaaaat cgggatattg ggtggcggaa tgattacccc aaatcgtcat ttgagtcact gcagtaatgt caacgcctgc ttttttagcc aattgagtac gagctcttaa ctcatccaat gtggtcattg cataaaaacg atcagaaggc acgtcttttg cgtgattcat agcaattagg caattggtat tgcaagggtt tcccacaaca aatacccgta catcatcact ggcataatca ttaatcgcct gaccttgttt ggtgaatata ccaccattga tttgtaataa atcagaacgc tccatgcctt gctttcgcgg aactgaaccg actaataatg cccagttaac accatccatt gccttattca aatctgcagt acaaacaatg cgttttaata agggaaatgc gcaatcatca agctccatag cgactccttc cagtgaggga agtgccggct caagttcaag taaattcaat tccacttcag tatttggccc aaacatttga ccggaagcaa tacgaaacac caaggcataa cctatttgcc ctgctgcacc tgtgacagca actcttactc gattannnnn nnatatggtg gtggtggtgg tgagccatgg tatat
Expected Protein Sequence
MAHHHHHHMT NNRVRVAVTG AAGQIGYALV FRIASGQMFG PNTEVELNLL ELEPALPSLE GVAMELDDCA FPLLKRIVCT ADLNKAMDGV NWALLVGSVP RKQGMERSDL LQINGGIFTK QGQAINDYAS DDVRVFVVGN PCNTNCLIAM NHAKDVPSDR FYAMTTLDEL RARTQLAKKA GVDITAVTQM TIWGNHSATQ YPDFYNAKIN GTSAAQVIND ETWLKETFVS TVQQRGAAVI KARGSSSAAS AANAIITGVN HLVTDTPAGE SFSMCRRSKG EYGVDEGLIF SFPCRREHGE LKVVENLEFN DFGRERFNTT LNELRSERDT VKSLGLLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa ataatcgagt aagagttgct gtcacaggtg cagcagggca aataggttat gccttggtgt ttcgtattgc ttccggtcaa atgtttgggc caaatactga agtggaattg aatttacttg aacttgagcc ggcacttccc tcactggaag gagtcgctat ggagcttgat gattgcgcat ttcccttatt aaaacgcatt gtttgtactg cagatttgaa taaggcaatg gatggtgtta actgggcatt attagtcggt tcagttccgc gaaagcaagg catggagcgt tctgatttat tacaaatcaa tggtggtata ttcaccaaac aaggtcaggc gattaatgat tatgccagtg atgatgtacg ggtatttgtt gtgggaaacc cttgcaatac caattgccta attgctatga atcacgcaaa agacgtgcct tctgatcgtt tttatgcaat gaccacattg gatgagttaa gagctcgtac tcaattggct aaaaaagcag gcgttgacat tactgcagtg actcaaatga cgatttgggg taatcattcc gccacccaat atcccgattt ttataacgca aaaatcaatg gaacttctgc tgcgcaagtc attaatgatg aaacctggtt aaaagagact tttgtatcaa ccgtccagca acgaggagcc gcagtgatca aggcacgggg ttcatcttct gctgcttctg ccgccaatgc gataattaca ggagttaatc atttggttac ggatactcct gctggcgaat cgttttcaat gtgccgtcgc tcaaagggag agtacggagt cgatgaaggt ttgattttct ctttcccatg ccgaagagaa catggcgaat taaaagtggt cgagaacttg gagttcaacg attttggccg cgagagattc aataccactt taaacgaatt acgaagtgag cgtgacacgg tcaagtcttt aggcttattg gattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for LepnA.01212.a.B1.PS38424
| PURIFICATION DATe: | 3/21/2018 |
| CONCENTRATION: | 82.9mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 72 |
| PERCENT COVERAGE: | 3 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHXX X
Validated NT Sequence
ctctcgcggc caaaatcgtt gaactccaag ttctcgacca cttttaattc gccatgttct cttcggcatg ggaaagagaa aatcaaacct tcatcgactc cgtactctcc ctttgagcga cggcacattg aaaacgattc gccagcagga gtatccgtaa ccaaatgatt aactcctgta attatcgcat tggcggcaga agcagcagaa gatgaacccc gtgccttgat cactgcggct cctcgttgct ggacggttga tacaaaagtc tcttttaacc aggtttcatc attaatgact tgcgcagcag aagttccatt gatttttgcg ttataaaaat cgggatattg ggtggcggaa tgattacccc aaatcgtcat ttgagtcact gcagtaatgt caacgcctgc ttttttagcc aattgagtac gagctcttaa ctcatccaat gtggtcattg cataaaaacg atcagaaggc acgtcttttg cgtgattcat agcaattagg caattggtat tgcaagggtt tcccacaaca aatacccgta catcatcact ggcataatca ttaatcgcct gaccttgttt ggtgaatata ccaccattga tttgtaataa atcagaacgc tccatgcctt gctttcgcgg aactgaaccg actaataatg cccagttaac accatccatt gccttattca aatctgcagt acaaacaatg cgttttaata agggaaatgc gcaatcatca agctccatag cgactccttc cagtgaggga agtgccggct caagttcaag taaattcaat tccacttcag tatttggccc aaacatttga ccggaagcaa tacgaaacac caaggcataa cctatttgcc ctgctgcacc tgtgacagca actcttactc gattannnnn nnatatggtg gtggtggtgg tgagccatgg tatat
Expressed Protein Sequence
MAHHHHHHMT NNRVRVAVTG AAGQIGYALV FRIASGQMFG PNTEVELNLL ELEPALPSLE GVAMELDDCA FPLLKRIVCT ADLNKAMDGV NWALLVGSVP RKQGMERSDL LQINGGIFTK QGQAINDYAS DDVRVFVVGN PCNTNCLIAM NHAKDVPSDR FYAMTTLDEL RARTQLAKKA GVDITAVTQM TIWGNHSATQ YPDFYNAKIN GTSAAQVIND ETWLKETFVS TVQQRGAAVI KARGSSSAAS AANAIITGVN HLVTDTPAGE SFSMCRRSKG EYGVDEGLIF SFPCRREHGE LKVVENLEFN DFGRERFNTT LNELRSERDT VKSLGLLD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA ATAATCGAGT AAGAGTTGCT GTCACAGGTG CAGCAGGGCA AATAGGTTAT GCCTTGGTGT TTCGTATTGC TTCCGGTCAA ATGTTTGGGC CAAATACTGA AGTGGAATTG AATTTACTTG AACTTGAGCC GGCACTTCCC TCACTGGAAG GAGTCGCTAT GGAGCTTGAT GATTGCGCAT TTCCCTTATT AAAACGCATT GTTTGTACTG CAGATTTGAA TAAGGCAATG GATGGTGTTA ACTGGGCATT ATTAGTCGGT TCAGTTCCGC GAAAGCAAGG CATGGAGCGT TCTGATTTAT TACAAATCAA TGGTGGTATA TTCACCAAAC AAGGTCAGGC GATTAATGAT TATGCCAGTG ATGATGTACG GGTATTTGTT GTGGGAAACC CTTGCAATAC CAATTGCCTA ATTGCTATGA ATCACGCAAA AGACGTGCCT TCTGATCGTT TTTATGCAAT GACCACATTG GATGAGTTAA GAGCTCGTAC TCAATTGGCT AAAAAAGCAG GCGTTGACAT TACTGCAGTG ACTCAAATGA CGATTTGGGG TAATCATTCC GCCACCCAAT ATCCCGATTT TTATAACGCA AAAATCAATG GAACTTCTGC TGCGCAAGTC ATTAATGATG AAACCTGGTT AAAAGAGACT TTTGTATCAA CCGTCCAGCA ACGAGGAGCC GCAGTGATCA AGGCACGGGG TTCATCTTCT GCTGCTTCTG CCGCCAATGC GATAATTACA GGAGTTAATC ATTTGGTTAC GGATACTCCT GCTGGCGAAT CGTTTTCAAT GTGCCGTCGC TCAAAGGGAG AGTACGGAGT CGATGAAGGT TTGATTTTCT CTTTCCCATG CCGAAGAGAA CATGGCGAAT TAAAAGTGGT CGAGAACTTG GAGTTCAACG ATTTTGGCCG CGAGAGATTC AATACCACTT TAAACGAATT ACGAAGTGAG CGTGACACGG TCAAGTCTTT AGGCTTATTG GATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT