LepnA.01128.a
Fumarate hydratase class II
| CENTER ID: | LepnA.01128.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01128.a.B1.GE41447 | Full length(LepnA.01128.a) | 1 | 464 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01128.a.B1.PS38406 | Full length(LepnA.01128.a) | 1 | 464 |
Structures
6N1M
DEPOSITED: 11/9/2018
DETERMINATION: XRay
CLONE: LepnA.01128.a.B1.GE41447
PROTEIN: LepnA.01128.a.B1.PS38406
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272624.6.peg.3137 |
| UniProt: | Q5ZRE5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKTRVETDS MGEIEVPSDK YWGAQTERSL HHFNIGKDIM PREVTHAFGI LKKAAALTNL ELGKLPKDKA DLIVQAAEEV SKGLLDEHFP LHVWQTGSGT QSNMNANEVI SNRAIELAGG TLGSKSPIHP NDHVNMSQSS NDTFPTAMHI AAAIAFNEKL LPAVRNLRHV FAAKMDAFKN IIKIGRTHLQ DAVPLTLGQE FSGYVAQLDA CVHRLEEVLP ELYELAAGGT AVGTGLNTHP QFAVKVAEHI AKITKLPFVT APNKFAALAS HEPLVHAHGA MKTLACALMK IANDIRWLAS GPRCGIGELI IPENEPGSSI MPGKVNPTQC EAMTMVCAQV LGNDTAVGIA DSQGNFELNV FKPVIIFNVL HSLNLLADSC HSFQEFCAEG IEPNQPVIDY YLHHSLMLVT ALNQHIGYDK AAKIAKTAHH DNISLQEAAV KLGILTAEQF AEFVKPEKMI SPEQ
NT Sequence
ATGGCTAAAA CACGTGTAGA AACAGACAGC ATGGGTGAAA TTGAAGTCCC GTCTGATAAA TATTGGGGCG CCCAAACCGA ACGTTCATTG CATCACTTCA ATATAGGCAA AGACATTATG CCACGCGAAG TAACCCATGC CTTTGGTATC CTGAAAAAGG CAGCCGCCCT GACGAATCTT GAATTAGGCA AACTGCCAAA AGACAAAGCG GATTTGATTG TTCAGGCAGC AGAAGAAGTG AGCAAAGGAC TATTGGATGA GCATTTTCCC TTGCATGTCT GGCAAACGGG AAGCGGCACA CAATCGAATA TGAACGCCAA TGAAGTCATT TCCAATCGGG CCATTGAGCT GGCTGGCGGC ACCCTGGGAA GCAAGTCCCC TATTCATCCC AATGATCACG TCAATATGTC TCAATCATCG AATGACACCT TCCCAACAGC CATGCATATT GCAGCGGCGA TAGCTTTTAA CGAAAAATTG TTACCTGCTG TACGCAATTT ACGTCATGTT TTCGCAGCGA AAATGGACGC TTTCAAAAAC ATCATAAAAA TTGGCAGAAC CCATTTGCAA GATGCGGTTC CACTGACTTT GGGGCAAGAG TTTTCAGGTT ATGTTGCCCA ACTGGATGCG TGCGTTCACC GTCTGGAAGA AGTCTTGCCT GAATTATATG AGCTGGCTGC CGGGGGCACT GCCGTTGGAA CCGGATTGAA CACCCATCCG CAATTCGCAG TCAAAGTTGC TGAACACATC GCTAAAATAA CCAAACTGCC TTTTGTGACC GCTCCTAACA AATTTGCCGC TCTGGCTTCT CACGAACCTC TGGTGCATGC GCACGGTGCC ATGAAAACTC TCGCCTGCGC ATTAATGAAA ATCGCCAATG ACATACGCTG GCTCGCCTCA GGACCTCGTT GCGGGATTGG TGAGCTGATT ATTCCAGAAA ATGAGCCAGG CTCATCCATC ATGCCAGGCA AAGTCAACCC GACACAATGC GAAGCCATGA CCATGGTTTG TGCCCAGGTA CTGGGTAATG ATACAGCCGT TGGAATTGCT GACAGCCAGG GTAATTTTGA ATTAAACGTG TTTAAACCGG TTATCATATT TAACGTATTG CACTCATTAA ACCTGCTCGC CGACAGCTGC CATTCCTTCC AGGAGTTTTG CGCAGAAGGA ATAGAGCCTA ATCAGCCGGT GATTGATTAC TATTTACACC ATTCCCTCAT GCTGGTTACT GCCCTTAATC AACACATTGG TTATGATAAA GCGGCTAAAA TCGCCAAAAC CGCTCATCAT GACAATATTT CATTGCAAGA AGCCGCCGTG AAACTGGGAA TTTTAACCGC CGAGCAATTT GCTGAGTTTG TGAAACCGGA AAAGATGATT TCCCCTGAGC AA
Details for LepnA.01128.a.B1.GE41447
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 51kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMA KTRVETDSMG EIEVPSDKYW GAQTERSLHH FNIGKDIMPR EVTHAFGILK KAAALTNLEL GKLPKDKADL IVQAAEEVSK GLLDEHFPLH VWQTGSGTQS NMNANEVISN RAIELAGGTL GSKSPIHPND HVNMSQSSND TFPTAMHIAA AIAFNEKLLP AVRNLRHVFA AKMDAFKNII KIGRTHLQDA VPLTLGQEFS GYVAQLDACV HRLEEVLPEL YELAAGGTAV GTGLNTHPQF AVKVAEHIAK ITKLPFVTAP NKFAALASHE PLVHAHGAMK TLACALMKIA NDIRWLASGP RCGIGELIIP ENEPGSSIMP GKVNPTQCEA MTMVCAQVLG NDTAVGIADS QGNFELNVFK PVIIFNVLHS LNLLADSCHS FQEFCAEGIE PNQPVIDYYL HHSLMLVTAL NQHIGYDKAA KIAKTAHHDN ISLQEAAVKL GILTAEQFAE FVKP
Validated NT Sequence
atggctcacc accaccacca ccatatggct aaaacacgtg tagaaacaga cagcatgggt gaaattgaag tcccgtctga taaatattgg ggcgcccaaa ccgaacgttc attgcatcac ttcaatatag gcaaagacat tatgccacgc gaagtaaccc atgcctttgg tatcctgaaa aaggcagccg ccctgacgaa tcttgaatta ggcaaactgc caaaagacaa agcggatttg attgttcagg cagcagaaga agtgagcaaa ggactattgg atgagcattt tcccttgcat gtctggcaaa cgggaagcgg cacacaatcg aatatgaacg ccaatgaagt catttccaat cgggccattg agctggctgg cggcaccctg ggaagcaagt cccctattca tcccaatgat cacgtcaata tgtctcaatc atcgaatgac accttcccaa cagccatgca tattgcagcg gcgatagctt ttaacgaaaa attgttacct gctgtacgca atttacgtca tgttttcgca gcgaaaatgg acgctttcaa aaacatcata aaaattggca gaacccattt gcaagatgcg gttccactga ctttggggca agagttttca ggttatgttg cccaactgga tgcgtgcgtt caccgtctgg aagaagtctt gcctgaatta tatgagctgg ctgccggggg cactgccgtt ggaaccggat tgaacaccca tccgcaattc gcagtcaaag ttgctgaaca catcgctaaa ataaccaaac tgccttttgt gaccgctcct aacaaatttg ccgctctggc ttctcacgaa cctctggtgc atgcgcacgg tgccatgaaa actctcgcct gcgcattaat gaaaatcgcc aatgacatac gctggctcgc ctcaggacct cgttgcggga ttggtgagct gattattcca gaaaatgagc caggctcatc catcatgcca ggcaaagtca acccgacaca atgcgaagcc atgaccatgg tttgtgccca ggtactgggt aatgatacag ccgttggaat tgctgacagc cagggtaatt ttgaattaaa cgtgtttaaa ccggttatca tatttaacgt attgcactca ttaaacctgc tcgccgacag ctgccattcc ttccaggagt tttgcgcaga aggaatagag cctaatcagc cggtgattga ttactattta caccattccc tcatgctggt tactgccctt aatcaacaca ttggttatga taaagcggct aaaatcgcca aaaccgctca tcatgacaat atttcattgc aagaagccgc cgtgaaactg ggaattttaa ccgccgagca atttgctgag tttgtgaaac cgg
Expected Protein Sequence
MAHHHHHHMA KTRVETDSMG EIEVPSDKYW GAQTERSLHH FNIGKDIMPR EVTHAFGILK KAAALTNLEL GKLPKDKADL IVQAAEEVSK GLLDEHFPLH VWQTGSGTQS NMNANEVISN RAIELAGGTL GSKSPIHPND HVNMSQSSND TFPTAMHIAA AIAFNEKLLP AVRNLRHVFA AKMDAFKNII KIGRTHLQDA VPLTLGQEFS GYVAQLDACV HRLEEVLPEL YELAAGGTAV GTGLNTHPQF AVKVAEHIAK ITKLPFVTAP NKFAALASHE PLVHAHGAMK TLACALMKIA NDIRWLASGP RCGIGELIIP ENEPGSSIMP GKVNPTQCEA MTMVCAQVLG NDTAVGIADS QGNFELNVFK PVIIFNVLHS LNLLADSCHS FQEFCAEGIE PNQPVIDYYL HHSLMLVTAL NQHIGYDKAA KIAKTAHHDN ISLQEAAVKL GILTAEQFAE FVKPEKMISP EQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aaacacgtgt agaaacagac agcatgggtg aaattgaagt cccgtctgat aaatattggg gcgcccaaac cgaacgttca ttgcatcact tcaatatagg caaagacatt atgccacgcg aagtaaccca tgcctttggt atcctgaaaa aggcagccgc cctgacgaat cttgaattag gcaaactgcc aaaagacaaa gcggatttga ttgttcaggc agcagaagaa gtgagcaaag gactattgga tgagcatttt cccttgcatg tctggcaaac gggaagcggc acacaatcga atatgaacgc caatgaagtc atttccaatc gggccattga gctggctggc ggcaccctgg gaagcaagtc ccctattcat cccaatgatc acgtcaatat gtctcaatca tcgaatgaca ccttcccaac agccatgcat attgcagcgg cgatagcttt taacgaaaaa ttgttacctg ctgtacgcaa tttacgtcat gttttcgcag cgaaaatgga cgctttcaaa aacatcataa aaattggcag aacccatttg caagatgcgg ttccactgac tttggggcaa gagttttcag gttatgttgc ccaactggat gcgtgcgttc accgtctgga agaagtcttg cctgaattat atgagctggc tgccgggggc actgccgttg gaaccggatt gaacacccat ccgcaattcg cagtcaaagt tgctgaacac atcgctaaaa taaccaaact gccttttgtg accgctccta acaaatttgc cgctctggct tctcacgaac ctctggtgca tgcgcacggt gccatgaaaa ctctcgcctg cgcattaatg aaaatcgcca atgacatacg ctggctcgcc tcaggacctc gttgcgggat tggtgagctg attattccag aaaatgagcc aggctcatcc atcatgccag gcaaagtcaa cccgacacaa tgcgaagcca tgaccatggt ttgtgcccag gtactgggta atgatacagc cgttggaatt gctgacagcc agggtaattt tgaattaaac gtgtttaaac cggttatcat atttaacgta ttgcactcat taaacctgct cgccgacagc tgccattcct tccaggagtt ttgcgcagaa ggaatagagc ctaatcagcc ggtgattgat tactatttac accattccct catgctggtt actgccctta atcaacacat tggttatgat aaagcggcta aaatcgccaa aaccgctcat catgacaata tttcattgca agaagccgcc gtgaaactgg gaattttaac cgccgagcaa tttgctgagt ttgtgaaacc ggaaaagatg atttcccctg agcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LepnA.01128.a.B1.PS38406
| PURIFICATION DATe: | 1/30/2018 |
| CONCENTRATION: | 17.9mg/ml |
| OBSERVED MW: | 51kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KTRVETDSMG EIEVPSDKYW GAQTERSLHH FNIGKDIMPR EVTHAFGILK KAAALTNLEL GKLPKDKADL IVQAAEEVSK GLLDEHFPLH VWQTGSGTQS NMNANEVISN RAIELAGGTL GSKSPIHPND HVNMSQSSND TFPTAMHIAA AIAFNEKLLP AVRNLRHVFA AKMDAFKNII KIGRTHLQDA VPLTLGQEFS GYVAQLDACV HRLEEVLPEL YELAAGGTAV GTGLNTHPQF AVKVAEHIAK ITKLPFVTAP NKFAALASHE PLVHAHGAMK TLACALMKIA NDIRWLASGP RCGIGELIIP ENEPGSSIMP GKVNPTQCEA MTMVCAQVLG NDTAVGIADS QGNFELNVFK PVIIFNVLHS LNLLADSCHS FQEFCAEGIE PNQPVIDYYL HHSLMLVTAL NQHIGYDKAA KIAKTAHHDN ISLQEAAVKL GILTAEQFAE FVKP
Validated NT Sequence
atggctcacc accaccacca ccatatggct aaaacacgtg tagaaacaga cagcatgggt gaaattgaag tcccgtctga taaatattgg ggcgcccaaa ccgaacgttc attgcatcac ttcaatatag gcaaagacat tatgccacgc gaagtaaccc atgcctttgg tatcctgaaa aaggcagccg ccctgacgaa tcttgaatta ggcaaactgc caaaagacaa agcggatttg attgttcagg cagcagaaga agtgagcaaa ggactattgg atgagcattt tcccttgcat gtctggcaaa cgggaagcgg cacacaatcg aatatgaacg ccaatgaagt catttccaat cgggccattg agctggctgg cggcaccctg ggaagcaagt cccctattca tcccaatgat cacgtcaata tgtctcaatc atcgaatgac accttcccaa cagccatgca tattgcagcg gcgatagctt ttaacgaaaa attgttacct gctgtacgca atttacgtca tgttttcgca gcgaaaatgg acgctttcaa aaacatcata aaaattggca gaacccattt gcaagatgcg gttccactga ctttggggca agagttttca ggttatgttg cccaactgga tgcgtgcgtt caccgtctgg aagaagtctt gcctgaatta tatgagctgg ctgccggggg cactgccgtt ggaaccggat tgaacaccca tccgcaattc gcagtcaaag ttgctgaaca catcgctaaa ataaccaaac tgccttttgt gaccgctcct aacaaatttg ccgctctggc ttctcacgaa cctctggtgc atgcgcacgg tgccatgaaa actctcgcct gcgcattaat gaaaatcgcc aatgacatac gctggctcgc ctcaggacct cgttgcggga ttggtgagct gattattcca gaaaatgagc caggctcatc catcatgcca ggcaaagtca acccgacaca atgcgaagcc atgaccatgg tttgtgccca ggtactgggt aatgatacag ccgttggaat tgctgacagc cagggtaatt ttgaattaaa cgtgtttaaa ccggttatca tatttaacgt attgcactca ttaaacctgc tcgccgacag ctgccattcc ttccaggagt tttgcgcaga aggaatagag cctaatcagc cggtgattga ttactattta caccattccc tcatgctggt tactgccctt aatcaacaca ttggttatga taaagcggct aaaatcgcca aaaccgctca tcatgacaat atttcattgc aagaagccgc cgtgaaactg ggaattttaa ccgccgagca atttgctgag tttgtgaaac cgg
Expressed Protein Sequence
MAHHHHHHMA KTRVETDSMG EIEVPSDKYW GAQTERSLHH FNIGKDIMPR EVTHAFGILK KAAALTNLEL GKLPKDKADL IVQAAEEVSK GLLDEHFPLH VWQTGSGTQS NMNANEVISN RAIELAGGTL GSKSPIHPND HVNMSQSSND TFPTAMHIAA AIAFNEKLLP AVRNLRHVFA AKMDAFKNII KIGRTHLQDA VPLTLGQEFS GYVAQLDACV HRLEEVLPEL YELAAGGTAV GTGLNTHPQF AVKVAEHIAK ITKLPFVTAP NKFAALASHE PLVHAHGAMK TLACALMKIA NDIRWLASGP RCGIGELIIP ENEPGSSIMP GKVNPTQCEA MTMVCAQVLG NDTAVGIADS QGNFELNVFK PVIIFNVLHS LNLLADSCHS FQEFCAEGIE PNQPVIDYYL HHSLMLVTAL NQHIGYDKAA KIAKTAHHDN ISLQEAAVKL GILTAEQFAE FVKPEKMISP EQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA AAACACGTGT AGAAACAGAC AGCATGGGTG AAATTGAAGT CCCGTCTGAT AAATATTGGG GCGCCCAAAC CGAACGTTCA TTGCATCACT TCAATATAGG CAAAGACATT ATGCCACGCG AAGTAACCCA TGCCTTTGGT ATCCTGAAAA AGGCAGCCGC CCTGACGAAT CTTGAATTAG GCAAACTGCC AAAAGACAAA GCGGATTTGA TTGTTCAGGC AGCAGAAGAA GTGAGCAAAG GACTATTGGA TGAGCATTTT CCCTTGCATG TCTGGCAAAC GGGAAGCGGC ACACAATCGA ATATGAACGC CAATGAAGTC ATTTCCAATC GGGCCATTGA GCTGGCTGGC GGCACCCTGG GAAGCAAGTC CCCTATTCAT CCCAATGATC ACGTCAATAT GTCTCAATCA TCGAATGACA CCTTCCCAAC AGCCATGCAT ATTGCAGCGG CGATAGCTTT TAACGAAAAA TTGTTACCTG CTGTACGCAA TTTACGTCAT GTTTTCGCAG CGAAAATGGA CGCTTTCAAA AACATCATAA AAATTGGCAG AACCCATTTG CAAGATGCGG TTCCACTGAC TTTGGGGCAA GAGTTTTCAG GTTATGTTGC CCAACTGGAT GCGTGCGTTC ACCGTCTGGA AGAAGTCTTG CCTGAATTAT ATGAGCTGGC TGCCGGGGGC ACTGCCGTTG GAACCGGATT GAACACCCAT CCGCAATTCG CAGTCAAAGT TGCTGAACAC ATCGCTAAAA TAACCAAACT GCCTTTTGTG ACCGCTCCTA ACAAATTTGC CGCTCTGGCT TCTCACGAAC CTCTGGTGCA TGCGCACGGT GCCATGAAAA CTCTCGCCTG CGCATTAATG AAAATCGCCA ATGACATACG CTGGCTCGCC TCAGGACCTC GTTGCGGGAT TGGTGAGCTG ATTATTCCAG AAAATGAGCC AGGCTCATCC ATCATGCCAG GCAAAGTCAA CCCGACACAA TGCGAAGCCA TGACCATGGT TTGTGCCCAG GTACTGGGTA ATGATACAGC CGTTGGAATT GCTGACAGCC AGGGTAATTT TGAATTAAAC GTGTTTAAAC CGGTTATCAT ATTTAACGTA TTGCACTCAT TAAACCTGCT CGCCGACAGC TGCCATTCCT TCCAGGAGTT TTGCGCAGAA GGAATAGAGC CTAATCAGCC GGTGATTGAT TACTATTTAC ACCATTCCCT CATGCTGGTT ACTGCCCTTA ATCAACACAT TGGTTATGAT AAAGCGGCTA AAATCGCCAA AACCGCTCAT CATGACAATA TTTCATTGCA AGAAGCCGCC GTGAAACTGG GAATTTTAAC CGCCGAGCAA TTTGCTGAGT TTGTGAAACC GGAAAAGATG ATTTCCCCTG AGCAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT