SSGCID: Target Page

LepnA.01067.a

DNA-directed RNA polymerase subunit alpha

CENTER ID:	LepnA.01067.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.01067.a.B1.GE41445	Full length(LepnA.01067.a)		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272624.6.peg.361
UniProt:	Q5ZYL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYTEINEMLT PKVLKVQAES PYKARIVLEP LERGFGHTLG NALRRILLSS MPGSAITEAS IDGVLHEYST IEGVQEDVVD LLLNLKSVAI KLTVGNEAQI TLNKEGPCQV TAGDIQLTHG QEIINPELVI ANLNEKGKLN MTLKVERGIG FHNTDAFVRY HDDEIEKKTV GKLKIDNSFS PVKKVAYFVD SARVENRTDL DKLTIELETN GTIDAEEAIR ISASILQRQL HAFVDMKFEE SRADNKERND FDPVLLRSVD DLELTVRSAN CLKAENIHYI GDLVQRTESE LLKTPNLGKK SLTEIKDVLA SRSLSLGMKL ENWPPASLGE

NT Sequence

ATGTATACTG AAATCAATGA AATGTTGACG CCTAAGGTTC TCAAAGTACA GGCCGAGTCG CCCTATAAAG CTAGAATAGT TTTAGAACCT TTGGAGCGAG GTTTTGGTCA CACCCTCGGC AATGCTTTGA GAAGAATCTT ATTGTCATCC ATGCCAGGAA GTGCCATTAC TGAAGCTTCT ATCGATGGTG TTTTGCATGA ATATAGTACT ATTGAAGGTG TTCAGGAAGA TGTGGTTGAT TTGTTGCTTA ACCTAAAATC AGTTGCTATC AAATTGACTG TAGGAAACGA AGCGCAGATT ACCTTAAATA AAGAAGGACC ATGTCAAGTT ACAGCTGGTG ATATTCAGTT GACTCATGGT CAAGAAATTA TTAATCCAGA ACTTGTCATA GCCAATCTCA ATGAAAAGGG TAAATTAAAT ATGACTCTTA AAGTTGAAAG AGGTATTGGC TTTCATAATA CTGATGCATT TGTTCGCTAT CATGATGATG AAATTGAGAA AAAGACGGTT GGAAAGTTAA AAATTGACAA CAGTTTTTCT CCAGTGAAGA AAGTAGCTTA TTTCGTCGAC AGTGCACGTG TAGAAAACAG AACAGATTTA GATAAGCTTA CTATTGAATT AGAAACTAAC GGTACTATCG ATGCTGAAGA GGCAATAAGA ATATCTGCTA GTATACTACA AAGACAGCTT CATGCGTTTG TTGACATGAA ATTTGAAGAA TCACGTGCTG ACAATAAAGA GCGTAATGAT TTTGATCCTG TATTATTACG TTCAGTGGAT GATTTGGAGT TGACTGTTCG ATCAGCTAAT TGTTTAAAAG CTGAAAACAT TCATTATATA GGCGATTTGG TTCAAAGAAC AGAAAGTGAA TTATTGAAGA CACCTAATCT TGGTAAGAAA TCACTAACTG AAATTAAAGA CGTTTTAGCG TCTCGATCAT TATCGCTGGG TATGAAACTT GAGAATTGGC CGCCAGCAAG CCTTGGCGAG

Details for LepnA.01067.a.B1.GE41445

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	38kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMY TEINEMLTPK VLKVQAESPY KARIVLEPLE RGFGHTLGNA LRRILLSSMP GSAITEASID GVLHEYSTIE GVQEDVVDLL LNLKSVAIKL TVGNEAQITL NKEGPCQVTA GDIQLTHGQE IINPELVIAN LNEKGKLNMT LKVERGIGFH NTDAFVRYHD DEIEKKTVGK LKIDNSFSPV KKVAYFVDSA RVENRTDLDK LTIELETNGT IDAEEAIRIS ASILQRQLHA FVDMKFEESR ADNKERNDFD PVLLRSVDDL ELTVRSANCL KAENIHYIGD LVQRTESELL KTPNLGKKSL TEIKDVLASR SLSLGMKLEN WXXXKPW

Validated NT Sequence

atggctcacc accaccacca ccatatgtat actgaaatca atgaaatgtt gacgcctaag gttctcaaag tacaggccga gtcgccctat aaagctagaa tagttttaga acctttggag cgaggttttg gtcacaccct cggcaatgct ttgagaagaa tcttattgtc atccatgcca ggaagtgcca ttactgaagc ttctatcgat ggtgttttgc atgaatatag tactattgaa ggtgttcagg aagatgtggt tgatttgttg cttaacctaa aatcagttgc tatcaaattg actgtaggaa acgaagcgca gattacctta aataaagaag gaccatgtca agttacagct ggtgatattc agttgactca tggtcaagaa attattaatc cagaacttgt catagccaat ctcaatgaaa agggtaaatt aaatatgact cttaaagttg aaagaggtat tggctttcat aatactgatg catttgttcg ctatcatgat gatgaaattg agaaaaagac ggttggaaag ttaaaaattg acaacagttt ttctccagtg aagaaagtag cttatttcgt cgacagtgca cgtgtagaaa acagaacaga tttagataag cttactattg aattagaaac taacggtact atcgatgctg aagaggcaat aagaatatct gctagtatac tacaaagaca gcttcatgcg tttgttgaca tgaaatttga agaatcacgt gctgacaata aagagcgtaa tgattttgat cctgtattat tacgttcagt ggatgatttg gagttgactg ttcgatcagc taattgttta aaagctgaaa acattcatta tataggcgat ttggttcaaa gaacagaaag tgaattattg aagacaccta atcttggtaa gaaatcacta actgaaatta aagacgtttt agcgtctcga tcattatcgc tgggtatgaa acttgagaat tggnnnnnnn gcaagccttg gcga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata ctgaaatcaa tgaaatgttg acgcctaagg ttctcaaagt acaggccgag tcgccctata aagctagaat agttttagaa cctttggagc gaggttttgg tcacaccctc ggcaatgctt tgagaagaat cttattgtca tccatgccag gaagtgccat tactgaagct tctatcgatg gtgttttgca tgaatatagt actattgaag gtgttcagga agatgtggtt gatttgttgc ttaacctaaa atcagttgct atcaaattga ctgtaggaaa cgaagcgcag attaccttaa ataaagaagg accatgtcaa gttacagctg gtgatattca gttgactcat ggtcaagaaa ttattaatcc agaacttgtc atagccaatc tcaatgaaaa gggtaaatta aatatgactc ttaaagttga aagaggtatt ggctttcata atactgatgc atttgttcgc tatcatgatg atgaaattga gaaaaagacg gttggaaagt taaaaattga caacagtttt tctccagtga agaaagtagc ttatttcgtc gacagtgcac gtgtagaaaa cagaacagat ttagataagc ttactattga attagaaact aacggtacta tcgatgctga agaggcaata agaatatctg ctagtatact acaaagacag cttcatgcgt ttgttgacat gaaatttgaa gaatcacgtg ctgacaataa agagcgtaat gattttgatc ctgtattatt acgttcagtg gatgatttgg agttgactgt tcgatcagct aattgtttaa aagctgaaaa cattcattat ataggcgatt tggttcaaag aacagaaagt gaattattga agacacctaa tcttggtaag aaatcactaa ctgaaattaa agacgtttta gcgtctcgat cattatcgct gggtatgaaa cttgagaatt ggccgccagc aagccttggc gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat