LepnA.01024.a
Enolase
| CENTER ID: | LepnA.01024.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01024.a.B1.GE41443 | Full length(LepnA.01024.a) | 1 | 422 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.01024.a.B1.PS38390 | Full length(LepnA.01024.a) | 1 | 422 |
Structures
6NB2
DEPOSITED: 12/6/2018
DETERMINATION: XRay
CLONE: LepnA.01024.a.B1.GE41443
PROTEIN: LepnA.01024.a.B1.PS38390
6NBM
DEPOSITED: 12/7/2018
DETERMINATION: XRay
CLONE: LepnA.01024.a.B1.GE41443
PROTEIN: LepnA.01024.a.B1.PS38390
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272624.6.peg.2134 |
| UniProt: | Q5ZTX1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHKIQARE ILDSRGNPTI EADVTLTTGI IGRASVPSGA STGSREACEL RDNDPKRYAG KGVQKAVKHV NNEINQALQG LSVEDQENLD RILCQLDNTE NKSHLGANAI LATSLACARA RALSLNQPLY MTLNQGDMMT MPVPMMNILN GGAHADNNVD IQEFMIMPIG APDFPVALQM GTEIFHVLKS VLKKQGLNTA VGDEGGFAPN IQSNRQALDL LSEAIEKAGF RLGEDIVFAL DVAASELFNE GFYHMYSENQ KFDSHQLIEY YANLISSYPI VSIEDGLDEK DWSGWKQLTT HLGNKVQLVG DDLFVTNPKI LREGIAQGIA NAILIKVNQI GTLSETRQAI KLAYDNGYRC VMSHRSGETE DTFIADLAVA SGCGQIKTGS LCRTDRTAKY NQLLRINELA SLPYAGKNIL KR
NT Sequence
ATGCACATAC ATAAAATTCA AGCACGTGAA ATATTAGATT CTCGCGGTAA TCCAACTATT GAGGCAGACG TTACTCTCAC AACTGGCATT ATTGGAAGAG CCAGCGTTCC TTCAGGTGCT TCCACAGGAA GCAGGGAAGC CTGTGAACTT AGAGACAATG ATCCAAAACG ATACGCTGGC AAGGGTGTAC AAAAAGCAGT TAAACACGTT AATAACGAGA TAAATCAGGC ATTACAAGGA TTGTCGGTTG AGGATCAGGA GAACTTGGAT CGCATTTTAT GCCAATTGGA TAATACAGAA AACAAATCCC ATCTGGGAGC TAATGCTATC CTGGCTACTT CTCTGGCATG TGCCAGAGCA AGAGCATTGT CTCTTAATCA GCCATTGTAT ATGACCCTTA ATCAGGGTGA CATGATGACC ATGCCTGTTC CTATGATGAA TATTCTGAAT GGAGGCGCTC ATGCTGATAA TAATGTGGAT ATTCAAGAAT TTATGATCAT GCCAATCGGC GCTCCTGATT TTCCAGTTGC ATTGCAAATG GGAACTGAAA TTTTTCATGT ACTGAAATCT GTGTTAAAAA AGCAAGGATT AAATACCGCT GTTGGTGATG AAGGTGGTTT TGCACCTAAT ATTCAATCCA ATCGCCAAGC GCTGGATCTC CTTAGCGAAG CAATTGAAAA AGCAGGCTTT CGATTAGGGG AAGACATTGT ATTTGCCCTG GATGTTGCGG CTTCTGAGCT TTTTAATGAG GGCTTTTATC ATATGTATTC TGAAAATCAA AAATTTGATT CTCATCAACT CATAGAATAC TATGCAAATC TTATTTCCAG TTATCCCATA GTCAGTATTG AAGATGGACT GGACGAAAAA GACTGGTCAG GATGGAAGCA ATTAACCACC CATTTGGGAA ATAAAGTTCA GCTCGTCGGA GATGATTTAT TTGTAACTAA TCCCAAAATT CTGCGAGAAG GAATTGCTCA GGGAATTGCT AATGCCATAT TAATTAAAGT CAATCAAATA GGTACTTTAA GCGAAACCAG GCAAGCTATA AAGCTCGCAT ACGACAATGG ATACCGATGT GTCATGTCCC ATCGTTCAGG AGAAACAGAA GATACCTTCA TTGCTGATCT TGCAGTAGCC AGTGGTTGTG GCCAAATAAA AACCGGATCT TTGTGTCGAA CAGACAGGAC TGCGAAATAT AATCAACTAC TAAGAATCAA TGAATTGGCG TCTTTACCCT ATGCTGGAAA AAACATTCTA AAGAGA
Details for LepnA.01024.a.B1.GE41443
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMH IHKIQAREIX XSRGNPTIEA DVTLTTGIIG RASVPSGAST GSREACELXD NDPKRYAGKG VQKAVKHVNN EINQALQGLS VEDQENLDRI LCQLDNTENK SHLGANAILA TSLACARARA LSLNQPLYMT LNQGDMMTMP VPMMNILNGG AHADNNVDIQ EFMIMPIGAP DFPVALQMGT EIFHVLKSVL KKQGLNTAVG DEGGFAPNIQ SNRQALDLLS EAIEKAGFRL GEDIVFALDV AASELFNEGF YHMYSENQKF DSHQLIEYYA NLISSYPIVS IEDGLDEKDW SGWKQLTTHL GNKVQLVGDD LFVTNPKILR EGIAQGIANA ILIKVNQIGT LSETRQAIKL AYDNGYRCVM SHRSGETEDT FIADLAVASG CGQIKTGSLC RTDRTAKYNQ LLRINELASL PY
Validated NT Sequence
anntaccatg gctcaccacc accaccacca tatgcacata cataaaattc aagcacgtga aatattnnat tctcgcggta atccaactat tgaggcagac gttactctca caactggcat tattggaaga gccagcgttc cttcaggtgc ttccacagga agcagggaag cctgtgaact tnnngacaat gatccaaaac gatacgctgg caagggtgta caaaaagcag ttaaacacgt taataacgag ataaatcagg cattacaagg attgtcggtt gaggatcagg agaacttgga tcgcatttta tgccaattgg ataatacaga aaacaaatcc catctgggag ctaatgctat cctggctact tctctggcat gtgccagagc aagagcattg tctcttaatc agccattgta tatgaccctt aatcagggtg acatgatgac catgcctgtt cctatgatga atattctgaa tggaggcgct catgctgata ataatgtgga tattcaagaa tttatgatca tgccaatcgg cgctcctgat tttccagttg cattgcaaat gggaactgaa atttttcatg tactgaaatc tgtgttaaaa aagcaaggat taaataccgc tgttggtgat gaaggtggtt ttgcacctaa tattcaatcc aatcgccaag cgctggatct ccttagcgaa gcaattgaaa aagcaggctt tcgattaggg gaagacattg tatttgccct ggatgttgcg gcttctgagc tttttaatga gggcttttat catatgtatt ctgaaaatca aaaatttgat tctcatcaac tcatagaata ctatgcaaat cttatttcca gttatcccat agtcagtatt gaagatggac tggacgaaaa agactggtca ggatggaagc aattaaccac ccatttggga aataaagttc agctcgtcgg agatgattta tttgtaacta atcccaaaat tctgcgagaa ggaattgctc agggaattgc taatgccata ttaattaaag tcaatcaaat aggtacttta agcgaaacca ggcaagctat aaagctcgca tacgacaatg gataccgatg tgtcatgtcc catcgttcag gagaaacaga agataccttc attgctgatc ttgcagtagc cagtggttgt ggccaaataa aaaccggatc tttgtgtcga acagacagga ctgcgaaata taatcaacta ctaagaatca atgaattggc gtctttaccc tatg
Expected Protein Sequence
MAHHHHHHMH IHKIQAREIL DSRGNPTIEA DVTLTTGIIG RASVPSGAST GSREACELRD NDPKRYAGKG VQKAVKHVNN EINQALQGLS VEDQENLDRI LCQLDNTENK SHLGANAILA TSLACARARA LSLNQPLYMT LNQGDMMTMP VPMMNILNGG AHADNNVDIQ EFMIMPIGAP DFPVALQMGT EIFHVLKSVL KKQGLNTAVG DEGGFAPNIQ SNRQALDLLS EAIEKAGFRL GEDIVFALDV AASELFNEGF YHMYSENQKF DSHQLIEYYA NLISSYPIVS IEDGLDEKDW SGWKQLTTHL GNKVQLVGDD LFVTNPKILR EGIAQGIANA ILIKVNQIGT LSETRQAIKL AYDNGYRCVM SHRSGETEDT FIADLAVASG CGQIKTGSLC RTDRTAKYNQ LLRINELASL PYAGKNILKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca tacataaaat tcaagcacgt gaaatattag attctcgcgg taatccaact attgaggcag acgttactct cacaactggc attattggaa gagccagcgt tccttcaggt gcttccacag gaagcaggga agcctgtgaa cttagagaca atgatccaaa acgatacgct ggcaagggtg tacaaaaagc agttaaacac gttaataacg agataaatca ggcattacaa ggattgtcgg ttgaggatca ggagaacttg gatcgcattt tatgccaatt ggataataca gaaaacaaat cccatctggg agctaatgct atcctggcta cttctctggc atgtgccaga gcaagagcat tgtctcttaa tcagccattg tatatgaccc ttaatcaggg tgacatgatg accatgcctg ttcctatgat gaatattctg aatggaggcg ctcatgctga taataatgtg gatattcaag aatttatgat catgccaatc ggcgctcctg attttccagt tgcattgcaa atgggaactg aaatttttca tgtactgaaa tctgtgttaa aaaagcaagg attaaatacc gctgttggtg atgaaggtgg ttttgcacct aatattcaat ccaatcgcca agcgctggat ctccttagcg aagcaattga aaaagcaggc tttcgattag gggaagacat tgtatttgcc ctggatgttg cggcttctga gctttttaat gagggctttt atcatatgta ttctgaaaat caaaaatttg attctcatca actcatagaa tactatgcaa atcttatttc cagttatccc atagtcagta ttgaagatgg actggacgaa aaagactggt caggatggaa gcaattaacc acccatttgg gaaataaagt tcagctcgtc ggagatgatt tatttgtaac taatcccaaa attctgcgag aaggaattgc tcagggaatt gctaatgcca tattaattaa agtcaatcaa ataggtactt taagcgaaac caggcaagct ataaagctcg catacgacaa tggataccga tgtgtcatgt cccatcgttc aggagaaaca gaagatacct tcattgctga tcttgcagta gccagtggtt gtggccaaat aaaaaccgga tctttgtgtc gaacagacag gactgcgaaa tataatcaac tactaagaat caatgaattg gcgtctttac cctatgctgg aaaaaacatt ctaaagagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LepnA.01024.a.B1.PS38390
| PURIFICATION DATe: | 12/5/2017 |
| CONCENTRATION: | 38.1mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMH IHKIQAREIX XSRGNPTIEA DVTLTTGIIG RASVPSGAST GSREACELXD NDPKRYAGKG VQKAVKHVNN EINQALQGLS VEDQENLDRI LCQLDNTENK SHLGANAILA TSLACARARA LSLNQPLYMT LNQGDMMTMP VPMMNILNGG AHADNNVDIQ EFMIMPIGAP DFPVALQMGT EIFHVLKSVL KKQGLNTAVG DEGGFAPNIQ SNRQALDLLS EAIEKAGFRL GEDIVFALDV AASELFNEGF YHMYSENQKF DSHQLIEYYA NLISSYPIVS IEDGLDEKDW SGWKQLTTHL GNKVQLVGDD LFVTNPKILR EGIAQGIANA ILIKVNQIGT LSETRQAIKL AYDNGYRCVM SHRSGETEDT FIADLAVASG CGQIKTGSLC RTDRTAKYNQ LLRINELASL PY
Validated NT Sequence
anntaccatg gctcaccacc accaccacca tatgcacata cataaaattc aagcacgtga aatattnnat tctcgcggta atccaactat tgaggcagac gttactctca caactggcat tattggaaga gccagcgttc cttcaggtgc ttccacagga agcagggaag cctgtgaact tnnngacaat gatccaaaac gatacgctgg caagggtgta caaaaagcag ttaaacacgt taataacgag ataaatcagg cattacaagg attgtcggtt gaggatcagg agaacttgga tcgcatttta tgccaattgg ataatacaga aaacaaatcc catctgggag ctaatgctat cctggctact tctctggcat gtgccagagc aagagcattg tctcttaatc agccattgta tatgaccctt aatcagggtg acatgatgac catgcctgtt cctatgatga atattctgaa tggaggcgct catgctgata ataatgtgga tattcaagaa tttatgatca tgccaatcgg cgctcctgat tttccagttg cattgcaaat gggaactgaa atttttcatg tactgaaatc tgtgttaaaa aagcaaggat taaataccgc tgttggtgat gaaggtggtt ttgcacctaa tattcaatcc aatcgccaag cgctggatct ccttagcgaa gcaattgaaa aagcaggctt tcgattaggg gaagacattg tatttgccct ggatgttgcg gcttctgagc tttttaatga gggcttttat catatgtatt ctgaaaatca aaaatttgat tctcatcaac tcatagaata ctatgcaaat cttatttcca gttatcccat agtcagtatt gaagatggac tggacgaaaa agactggtca ggatggaagc aattaaccac ccatttggga aataaagttc agctcgtcgg agatgattta tttgtaacta atcccaaaat tctgcgagaa ggaattgctc agggaattgc taatgccata ttaattaaag tcaatcaaat aggtacttta agcgaaacca ggcaagctat aaagctcgca tacgacaatg gataccgatg tgtcatgtcc catcgttcag gagaaacaga agataccttc attgctgatc ttgcagtagc cagtggttgt ggccaaataa aaaccggatc tttgtgtcga acagacagga ctgcgaaata taatcaacta ctaagaatca atgaattggc gtctttaccc tatg
Expressed Protein Sequence
MAHHHHHHMH IHKIQAREIL DSRGNPTIEA DVTLTTGIIG RASVPSGAST GSREACELRD NDPKRYAGKG VQKAVKHVNN EINQALQGLS VEDQENLDRI LCQLDNTENK SHLGANAILA TSLACARARA LSLNQPLYMT LNQGDMMTMP VPMMNILNGG AHADNNVDIQ EFMIMPIGAP DFPVALQMGT EIFHVLKSVL KKQGLNTAVG DEGGFAPNIQ SNRQALDLLS EAIEKAGFRL GEDIVFALDV AASELFNEGF YHMYSENQKF DSHQLIEYYA NLISSYPIVS IEDGLDEKDW SGWKQLTTHL GNKVQLVGDD LFVTNPKILR EGIAQGIANA ILIKVNQIGT LSETRQAIKL AYDNGYRCVM SHRSGETEDT FIADLAVASG CGQIKTGSLC RTDRTAKYNQ LLRINELASL PYAGKNILKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACA TACATAAAAT TCAAGCACGT GAAATATTAG ATTCTCGCGG TAATCCAACT ATTGAGGCAG ACGTTACTCT CACAACTGGC ATTATTGGAA GAGCCAGCGT TCCTTCAGGT GCTTCCACAG GAAGCAGGGA AGCCTGTGAA CTTAGAGACA ATGATCCAAA ACGATACGCT GGCAAGGGTG TACAAAAAGC AGTTAAACAC GTTAATAACG AGATAAATCA GGCATTACAA GGATTGTCGG TTGAGGATCA GGAGAACTTG GATCGCATTT TATGCCAATT GGATAATACA GAAAACAAAT CCCATCTGGG AGCTAATGCT ATCCTGGCTA CTTCTCTGGC ATGTGCCAGA GCAAGAGCAT TGTCTCTTAA TCAGCCATTG TATATGACCC TTAATCAGGG TGACATGATG ACCATGCCTG TTCCTATGAT GAATATTCTG AATGGAGGCG CTCATGCTGA TAATAATGTG GATATTCAAG AATTTATGAT CATGCCAATC GGCGCTCCTG ATTTTCCAGT TGCATTGCAA ATGGGAACTG AAATTTTTCA TGTACTGAAA TCTGTGTTAA AAAAGCAAGG ATTAAATACC GCTGTTGGTG ATGAAGGTGG TTTTGCACCT AATATTCAAT CCAATCGCCA AGCGCTGGAT CTCCTTAGCG AAGCAATTGA AAAAGCAGGC TTTCGATTAG GGGAAGACAT TGTATTTGCC CTGGATGTTG CGGCTTCTGA GCTTTTTAAT GAGGGCTTTT ATCATATGTA TTCTGAAAAT CAAAAATTTG ATTCTCATCA ACTCATAGAA TACTATGCAA ATCTTATTTC CAGTTATCCC ATAGTCAGTA TTGAAGATGG ACTGGACGAA AAAGACTGGT CAGGATGGAA GCAATTAACC ACCCATTTGG GAAATAAAGT TCAGCTCGTC GGAGATGATT TATTTGTAAC TAATCCCAAA ATTCTGCGAG AAGGAATTGC TCAGGGAATT GCTAATGCCA TATTAATTAA AGTCAATCAA ATAGGTACTT TAAGCGAAAC CAGGCAAGCT ATAAAGCTCG CATACGACAA TGGATACCGA TGTGTCATGT CCCATCGTTC AGGAGAAACA GAAGATACCT TCATTGCTGA TCTTGCAGTA GCCAGTGGTT GTGGCCAAAT AAAAACCGGA TCTTTGTGTC GAACAGACAG GACTGCGAAA TATAATCAAC TACTAAGAAT CAATGAATTG GCGTCTTTAC CCTATGCTGG AAAAAACATT CTAAAGAGAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT