SSGCID: Target Page

LepnA.00983.a

Signal recognition particle receptor FtsY

CENTER ID:	LepnA.00983.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.00983.a.B1.GE41442	Full length(LepnA.00983.a)		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272624.6.peg.2850
UniProt:	Q5ZS49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIEFHIFKEL YTKMIKWFKR NQNSEPNLDA EEQQDHAKSS MESRLEEPQE LTVKEPSPKE SIFARFRKGL SKTRHQLGEG ISRLLLGKKE ISQELLEELE TLLISADLGL DTTQDVLKQL NEGLARKQLS NGEAVYLTLK TNLQTILSQE EKPLSIETED GSPFVILMVG VNGAGKTTTI GKLAKQFQKQ GKKVMLAAGD TFRAAAVEQL HVWGERNGIP VIAQHTGADS ASVIFDALQA AKARKIDVLI ADTAGRLHTQ SNLMEELKKV KRVMQKLDPK APHETMLVLD ASIGQNALAQ AKQFHEAVQL TGITMTKLDG TAKGGILFAI ANDLGIPFRY LGVGEGIEDL RPFDAAQFVS AIFDDDHI

NT Sequence

ATGATAGAAT TTCATATTTT TAAGGAATTG TACACTAAAA TGATTAAATG GTTTAAACGA AATCAAAATT CCGAACCAAA TTTGGATGCC GAAGAGCAAC AAGATCATGC CAAAAGCAGC ATGGAATCCA GGCTTGAAGA ACCCCAGGAA CTGACTGTCA AAGAGCCTTC GCCCAAAGAA AGTATTTTTG CCCGGTTTAG GAAAGGCCTT AGCAAAACCC GTCATCAATT GGGGGAAGGC ATAAGTCGGC TGCTGTTAGG TAAAAAAGAA ATCAGCCAGG AGTTACTCGA AGAGCTAGAG ACCTTATTAA TTAGTGCTGA TTTAGGTTTG GACACCACTC AAGACGTATT GAAACAGCTA AATGAAGGCT TGGCACGAAA GCAACTGTCT AATGGTGAAG CAGTTTATCT GACTCTAAAG ACAAATTTGC AAACTATTTT GAGCCAGGAA GAGAAACCGC TTTCCATAGA AACTGAAGAC GGCTCTCCCT TTGTCATATT AATGGTTGGT GTCAATGGAG CCGGTAAAAC AACCACGATT GGTAAACTAG CCAAACAATT CCAAAAGCAG GGAAAAAAAG TCATGTTAGC TGCGGGCGAT ACGTTTCGGG CGGCTGCGGT GGAACAATTG CATGTATGGG GCGAAAGAAA TGGCATACCT GTTATTGCCC AGCATACCGG CGCTGACAGT GCCTCAGTAA TATTTGATGC TCTTCAGGCT GCCAAAGCGA GAAAAATAGA TGTTTTAATC GCTGATACAG CCGGGCGTTT GCATACTCAA AGCAATTTAA TGGAAGAGTT GAAAAAAGTG AAAAGAGTCA TGCAGAAACT CGATCCCAAG GCACCTCATG AAACCATGTT GGTATTGGAT GCGAGTATTG GGCAGAACGC CTTGGCTCAG GCAAAGCAGT TTCATGAAGC AGTACAATTA ACTGGAATTA CTATGACCAA GCTTGATGGG ACAGCCAAAG GCGGGATATT ATTTGCTATA GCGAATGATT TGGGGATACC TTTTCGTTAT TTAGGGGTAG GGGAGGGCAT TGAAGATTTG AGGCCCTTTG ACGCAGCGCA ATTTGTTAGT GCGATATTCG ATGATGATCA CATT

Details for LepnA.00983.a.B1.GE41442

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	42kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI EFHIFKELYT KMIKWFKRNQ NSEPNLDAEE QQDHAKSSME SRLEEPQELT VKEPSPKESI FARFRKGLSK TRHQLGEGIS RLLLGKKEIS QELLEELETL LISADLGLDT TQDVLKQLNE GLARKQLSNG EAVYLTLKTN LQTILSQEEK PLSIETEDGS PFVILMVGVN GAGKTTTIGK LAKQFQKQGK KVMLAAGDTF RAAAVEQLHV WGERNGIPVI AQHTGADSAS VIFDALQAAK ARKIDVLIAD TAGRLHTQSN LMEELKKVKR VMQKLDPKAP HETMLVLDAS IGQNALAQAK QFHEAVQLTG ITMTKLDGTA KGGILFAIAN DLGIPFRYLG VGEGIEDLRP FDAAQFVS

Validated NT Sequence

atggctcacc accaccacca ccatatgata gaatttcata tttttaagga attgtacact aaaatgatta aatggtttaa acgaaatcaa aattccgaac caaatttgga tgccgaagag caacaagatc atgccaaaag cagcatggaa tccaggcttg aagaacccca ggaactgact gtcaaagagc cttcgcccaa agaaagtatt tttgcccggt ttaggaaagg ccttagcaaa acccgtcatc aattggggga aggcataagt cggctgctgt taggtaaaaa agaaatcagc caggagttac tcgaagagct agagacctta ttaattagtg ctgatttagg tttggacacc actcaagacg tattgaaaca gctaaatgaa ggcttggcac gaaagcaact gtctaatggt gaagcagttt atctgactct aaagacaaat ttgcaaacta ttttgagcca ggaagagaaa ccgctttcca tagaaactga agacggctct ccctttgtca tattaatggt tggtgtcaat ggagccggta aaacaaccac gattggtaaa ctagccaaac aattccaaaa gcagggaaaa aaagtcatgt tagctgcggg cgatacgttt cgggcggctg cggtggaaca attgcatgta tggggcgaaa gaaatggcat acctgttatt gcccagcata ccggcgctga cagtgcctca gtaatatttg atgctcttca ggctgccaaa gcgagaaaaa tagatgtttt aatcgctgat acagccgggc gtttgcatac tcaaagcaat ttaatggaag agttgaaaaa agtgaaaaga gtcatgcaga aactcgatcc caaggcacct catgaaacca tgttggtatt ggatgcgagt attgggcaga acgccttggc tcaggcaaag cagtttcatg aagcagtaca attaactgga attactatga ccaagcttga tgggacagcc aaaggcggga tattatttgc tatagcgaat gatttgggga taccttttcg ttatttaggg gtaggggagg gcattgaaga tttgaggccc tttgacgcag cgcaatttgt tagtgcg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatag aatttcatat ttttaaggaa ttgtacacta aaatgattaa atggtttaaa cgaaatcaaa attccgaacc aaatttggat gccgaagagc aacaagatca tgccaaaagc agcatggaat ccaggcttga agaaccccag gaactgactg tcaaagagcc ttcgcccaaa gaaagtattt ttgcccggtt taggaaaggc cttagcaaaa cccgtcatca attgggggaa ggcataagtc ggctgctgtt aggtaaaaaa gaaatcagcc aggagttact cgaagagcta gagaccttat taattagtgc tgatttaggt ttggacacca ctcaagacgt attgaaacag ctaaatgaag gcttggcacg aaagcaactg tctaatggtg aagcagttta tctgactcta aagacaaatt tgcaaactat tttgagccag gaagagaaac cgctttccat agaaactgaa gacggctctc cctttgtcat attaatggtt ggtgtcaatg gagccggtaa aacaaccacg attggtaaac tagccaaaca attccaaaag cagggaaaaa aagtcatgtt agctgcgggc gatacgtttc gggcggctgc ggtggaacaa ttgcatgtat ggggcgaaag aaatggcata cctgttattg cccagcatac cggcgctgac agtgcctcag taatatttga tgctcttcag gctgccaaag cgagaaaaat agatgtttta atcgctgata cagccgggcg tttgcatact caaagcaatt taatggaaga gttgaaaaaa gtgaaaagag tcatgcagaa actcgatccc aaggcacctc atgaaaccat gttggtattg gatgcgagta ttgggcagaa cgccttggct caggcaaagc agtttcatga agcagtacaa ttaactggaa ttactatgac caagcttgat gggacagcca aaggcgggat attatttgct atagcgaatg atttggggat accttttcgt tatttagggg taggggaggg cattgaagat ttgaggccct ttgacgcagc gcaatttgtt agtgcgatat tcgatgatga tcacatttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at