LepnA.00888.a
Adenylosuccinate synthetase
| CENTER ID: | LepnA.00888.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00888.a.B1.GE41437 | Full length(LepnA.00888.a) | 1 | 431 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00888.a.B1.PW38319 | Full length(LepnA.00888.a) | 1 | 431 |
Structures
6BS7
DEPOSITED: 12/1/2017
DETERMINATION: XRay
CLONE: LepnA.00888.a.B1.GE41437
PROTEIN: LepnA.00888.a.B1.PW38319
6C25
DEPOSITED: 1/6/2018
DETERMINATION: XRay
CLONE: LepnA.00888.a.B1.GE41437
PROTEIN: LepnA.00888.a.B1.PW38319
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.506 |
| UniProt: | Q5ZY86 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKNVVVLGT QWGDEGKGKI VDLLTQDAQV VVRYQGGHNA GHTLKINGVK TVLRLIPSGM LRPNVTCYIA NGVVLSPQAL LSEIKELEGN GINVRERLRI SLACPLILPY HIALDKARET HMGKSAIGTT GRGIGPAYED KVARRALRVG DLFHRDRFAN KLTELLDYHN FVLTQYFKQP AVDLESLLGE SLQWAEELRP MVCDVSACLH EHRKQGENIL FEGAQGVYLD IDHGTYPYVT SSNTCVGSVI NGAGFGPRYI DYVLGITKAY TTRVGGGPFP TELLDDVGKR IAERGQEFGA VTGRPRRCGW FDAVLLKRSI ELNSISGLCV TKLDVLDGLE VLRIAVAYKD RDGNILSRPP LAADDFNDLL PVYEELPGWQ ESTADVTVMS DLPANARAYL KRIEEILGIP IDMLSTGPER DSTITLRGPF L
NT Sequence
ATGGGTAAGA ATGTTGTTGT TTTGGGTACA CAATGGGGAG ATGAAGGCAA AGGCAAAATT GTTGATTTGC TGACACAAGA TGCTCAAGTG GTTGTCCGTT ATCAAGGGGG CCATAATGCA GGCCATACAT TAAAAATCAA TGGAGTAAAA ACAGTACTTC GTTTAATTCC ATCCGGGATG CTTCGCCCTA ACGTAACCTG CTATATTGCA AATGGTGTGG TTTTATCACC TCAGGCATTA CTTAGTGAAA TTAAGGAGCT TGAGGGTAAT GGCATCAATG TGAGGGAGCG ATTACGCATT AGCCTGGCTT GCCCCCTGAT TCTCCCTTAC CACATTGCTT TGGATAAGGC TCGTGAGACA CATATGGGCA AGTCTGCAAT TGGAACAACA GGTCGTGGTA TAGGTCCTGC TTATGAGGAT AAAGTGGCTC GGCGCGCTTT AAGAGTAGGT GATTTATTTC ATAGAGACCG ATTTGCCAAT AAATTAACAG AATTATTGGA TTATCATAAT TTTGTATTAA CACAATATTT CAAACAACCA GCTGTTGATT TGGAATCCCT ATTGGGTGAG TCATTACAAT GGGCTGAAGA ATTAAGACCA ATGGTTTGCG ATGTTTCTGC ATGTTTGCAT GAACATAGAA AACAAGGTGA AAATATTTTA TTCGAAGGGG CGCAAGGTGT TTATTTAGAT ATTGATCATG GAACATACCC CTACGTAACT TCTTCAAATA CCTGTGTGGG ATCTGTAATT AATGGTGCGG GATTTGGTCC TCGCTATATT GATTACGTGT TAGGCATTAC AAAAGCTTAC ACAACTCGAG TTGGCGGTGG CCCATTCCCC ACTGAATTAC TTGATGACGT TGGAAAACGC ATTGCGGAAC GAGGACAGGA ATTCGGCGCA GTAACTGGTA GACCCAGACG TTGTGGGTGG TTTGACGCTG TTTTATTAAA ACGCTCGATT GAGTTAAATA GTATTTCAGG ACTCTGTGTT ACCAAATTGG ATGTTCTCGA TGGCTTAGAG GTATTACGGA TTGCTGTGGC TTATAAAGAC AGGGATGGCA ATATATTATC TCGACCTCCT CTGGCAGCTG ATGATTTCAA CGATTTGTTA CCAGTCTATG AGGAGCTTCC TGGTTGGCAG GAATCTACTG CTGATGTGAC TGTGATGAGT GACTTACCCG CCAATGCAAG GGCTTATCTA AAGCGAATTG AAGAGATATT AGGGATTCCT ATTGATATGC TATCAACAGG ACCTGAAAGG GATTCAACAA TTACTTTACG AGGTCCTTTT CTT
Details for LepnA.00888.a.B1.GE41437
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTQDAQVVV RYQGGHNAGH TLKINGVKTV LRLIPSGMLR PNVTCYIANG VVLSPQALLX EIKELEGNGI NVRERLRISL ACPLILPYHI ALDKARETHM GKSAIGTTGR GIGPAYEDKV ARRALRVGDL FHRDRFANKL TELLDYHNFV LTQYFKQPAV DLESLLGESL QWAEELRPMV CDVSACLHEH RKQGENILFE GAQGVYLDID HGTYPYVTSS NTCVGSVING AGFGPRYIDY VLGITKAYTT RVGGGPFPTE LLDDVGKRIA ERGQEFGAVT GRPRRCGWFD AVLLKRSIEL NSISGLCVTK LDVLDGLEVL RIAVAYKDRD GNILSRPPXA ADDFNDLLPV YEELPGWQES TADVTVMSDL PANARAYLKR IEEILGIPID MLSTGPERDS
Validated NT Sequence
atggctcacc accaccacca ccatatgggt aagaatgttg ttgttttggg tacacaatgg ggagatgaag gcaaaggcaa aattgttgat ttgctgacac aagatgctca agtggttgtc cgttatcaag ggggccataa tgcaggccat acattaaaaa tcaatggagt aaaaacagta cttcgtttaa ttccatccgg gatgcttcgc cctaacgtaa cctgctatat tgcaaatggt gtggttttat cacctcaggc attacttngt gaaattaagg agcttgaggg taatggcatc aatgtgaggg agcgattacg cattagcctg gcttgccccc tgattctccc ttaccacatt gctttggata aggctcgtga gacacatatg ggcaagtctg caattggaac aacaggtcgt ggtataggtc ctgcttatga ggataaagtg gctcggcgcg ctttaagagt aggtgattta tttcatagag accgatttgc caataaatta acagaattat tggattatca taattttgta ttaacacaat atttcaaaca accagctgtt gatttggaat ccctattggg tgagtcatta caatgggctg aagaattaag accaatggtt tgcgatgttt ctgcatgttt gcatgaacat agaaaacaag gtgaaaatat tttattcgaa ggggcgcaag gtgtttattt agatattgat catggaacat acccctacgt aacttcttca aatacctgtg tgggatctgt aattaatggt gcgggatttg gtcctcgcta tattgattac gtgttaggca ttacaaaagc ttacacaact cgagttggcg gtggcccatt ccccactgaa ttacttgatg acgttggaaa acgcattgcg gaacgaggac aggaattcgg cgcagtaact ggtagaccca gacgttgtgg gtggtttgac gctgttttat taaaacgctc gattgagtta aatagtattt caggactctg tgttaccaaa ttggatgttc tcgatggctt agaggtatta cggattgctg tggcttataa agacagggat ggcaatatat tatctcgacc tcctcnggca gctgatgatt tcaacgattt gttaccagtc tatgaggagc ttcctggttg gcaggaatct actgctgatg tgactgtgat gagtgactta cccgccaatg caagggctta tctaaagcga attgaagaga tattagggat tcctattgat atgctatcaa caggacctga aagggattca a
Expected Protein Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTQDAQVVV RYQGGHNAGH TLKINGVKTV LRLIPSGMLR PNVTCYIANG VVLSPQALLS EIKELEGNGI NVRERLRISL ACPLILPYHI ALDKARETHM GKSAIGTTGR GIGPAYEDKV ARRALRVGDL FHRDRFANKL TELLDYHNFV LTQYFKQPAV DLESLLGESL QWAEELRPMV CDVSACLHEH RKQGENILFE GAQGVYLDID HGTYPYVTSS NTCVGSVING AGFGPRYIDY VLGITKAYTT RVGGGPFPTE LLDDVGKRIA ERGQEFGAVT GRPRRCGWFD AVLLKRSIEL NSISGLCVTK LDVLDGLEVL RIAVAYKDRD GNILSRPPLA ADDFNDLLPV YEELPGWQES TADVTVMSDL PANARAYLKR IEEILGIPID MLSTGPERDS TITLRGPFL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta agaatgttgt tgttttgggt acacaatggg gagatgaagg caaaggcaaa attgttgatt tgctgacaca agatgctcaa gtggttgtcc gttatcaagg gggccataat gcaggccata cattaaaaat caatggagta aaaacagtac ttcgtttaat tccatccggg atgcttcgcc ctaacgtaac ctgctatatt gcaaatggtg tggttttatc acctcaggca ttacttagtg aaattaagga gcttgagggt aatggcatca atgtgaggga gcgattacgc attagcctgg cttgccccct gattctccct taccacattg ctttggataa ggctcgtgag acacatatgg gcaagtctgc aattggaaca acaggtcgtg gtataggtcc tgcttatgag gataaagtgg ctcggcgcgc tttaagagta ggtgatttat ttcatagaga ccgatttgcc aataaattaa cagaattatt ggattatcat aattttgtat taacacaata tttcaaacaa ccagctgttg atttggaatc cctattgggt gagtcattac aatgggctga agaattaaga ccaatggttt gcgatgtttc tgcatgtttg catgaacata gaaaacaagg tgaaaatatt ttattcgaag gggcgcaagg tgtttattta gatattgatc atggaacata cccctacgta acttcttcaa atacctgtgt gggatctgta attaatggtg cgggatttgg tcctcgctat attgattacg tgttaggcat tacaaaagct tacacaactc gagttggcgg tggcccattc cccactgaat tacttgatga cgttggaaaa cgcattgcgg aacgaggaca ggaattcggc gcagtaactg gtagacccag acgttgtggg tggtttgacg ctgttttatt aaaacgctcg attgagttaa atagtatttc aggactctgt gttaccaaat tggatgttct cgatggctta gaggtattac ggattgctgt ggcttataaa gacagggatg gcaatatatt atctcgacct cctctggcag ctgatgattt caacgatttg ttaccagtct atgaggagct tcctggttgg caggaatcta ctgctgatgt gactgtgatg agtgacttac ccgccaatgc aagggcttat ctaaagcgaa ttgaagagat attagggatt cctattgata tgctatcaac aggacctgaa agggattcaa caattacttt acgaggtcct tttctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for LepnA.00888.a.B1.PW38319
| PURIFICATION DATe: | 7/31/2017 |
| CONCENTRATION: | 36.92mg/ml |
| OBSERVED MW: | 56kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
UV lamp failed, no SEC chromatogram.
Fractions were pulled purely based on SDS-PAGE
Validated AA Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTQDAQVVV RYQGGHNAGH TLKINGVKTV LRLIPSGMLR PNVTCYIANG VVLSPQALLX EIKELEGNGI NVRERLRISL ACPLILPYHI ALDKARETHM GKSAIGTTGR GIGPAYEDKV ARRALRVGDL FHRDRFANKL TELLDYHNFV LTQYFKQPAV DLESLLGESL QWAEELRPMV CDVSACLHEH RKQGENILFE GAQGVYLDID HGTYPYVTSS NTCVGSVING AGFGPRYIDY VLGITKAYTT RVGGGPFPTE LLDDVGKRIA ERGQEFGAVT GRPRRCGWFD AVLLKRSIEL NSISGLCVTK LDVLDGLEVL RIAVAYKDRD GNILSRPPXA ADDFNDLLPV YEELPGWQES TADVTVMSDL PANARAYLKR IEEILGIPID MLSTGPERDS
Validated NT Sequence
atggctcacc accaccacca ccatatgggt aagaatgttg ttgttttggg tacacaatgg ggagatgaag gcaaaggcaa aattgttgat ttgctgacac aagatgctca agtggttgtc cgttatcaag ggggccataa tgcaggccat acattaaaaa tcaatggagt aaaaacagta cttcgtttaa ttccatccgg gatgcttcgc cctaacgtaa cctgctatat tgcaaatggt gtggttttat cacctcaggc attacttngt gaaattaagg agcttgaggg taatggcatc aatgtgaggg agcgattacg cattagcctg gcttgccccc tgattctccc ttaccacatt gctttggata aggctcgtga gacacatatg ggcaagtctg caattggaac aacaggtcgt ggtataggtc ctgcttatga ggataaagtg gctcggcgcg ctttaagagt aggtgattta tttcatagag accgatttgc caataaatta acagaattat tggattatca taattttgta ttaacacaat atttcaaaca accagctgtt gatttggaat ccctattggg tgagtcatta caatgggctg aagaattaag accaatggtt tgcgatgttt ctgcatgttt gcatgaacat agaaaacaag gtgaaaatat tttattcgaa ggggcgcaag gtgtttattt agatattgat catggaacat acccctacgt aacttcttca aatacctgtg tgggatctgt aattaatggt gcgggatttg gtcctcgcta tattgattac gtgttaggca ttacaaaagc ttacacaact cgagttggcg gtggcccatt ccccactgaa ttacttgatg acgttggaaa acgcattgcg gaacgaggac aggaattcgg cgcagtaact ggtagaccca gacgttgtgg gtggtttgac gctgttttat taaaacgctc gattgagtta aatagtattt caggactctg tgttaccaaa ttggatgttc tcgatggctt agaggtatta cggattgctg tggcttataa agacagggat ggcaatatat tatctcgacc tcctcnggca gctgatgatt tcaacgattt gttaccagtc tatgaggagc ttcctggttg gcaggaatct actgctgatg tgactgtgat gagtgactta cccgccaatg caagggctta tctaaagcga attgaagaga tattagggat tcctattgat atgctatcaa caggacctga aagggattca a
Expressed Protein Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTQDAQVVV RYQGGHNAGH TLKINGVKTV LRLIPSGMLR PNVTCYIANG VVLSPQALLS EIKELEGNGI NVRERLRISL ACPLILPYHI ALDKARETHM GKSAIGTTGR GIGPAYEDKV ARRALRVGDL FHRDRFANKL TELLDYHNFV LTQYFKQPAV DLESLLGESL QWAEELRPMV CDVSACLHEH RKQGENILFE GAQGVYLDID HGTYPYVTSS NTCVGSVING AGFGPRYIDY VLGITKAYTT RVGGGPFPTE LLDDVGKRIA ERGQEFGAVT GRPRRCGWFD AVLLKRSIEL NSISGLCVTK LDVLDGLEVL RIAVAYKDRD GNILSRPPLA ADDFNDLLPV YEELPGWQES TADVTVMSDL PANARAYLKR IEEILGIPID MLSTGPERDS TITLRGPFL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTA AGAATGTTGT TGTTTTGGGT ACACAATGGG GAGATGAAGG CAAAGGCAAA ATTGTTGATT TGCTGACACA AGATGCTCAA GTGGTTGTCC GTTATCAAGG GGGCCATAAT GCAGGCCATA CATTAAAAAT CAATGGAGTA AAAACAGTAC TTCGTTTAAT TCCATCCGGG ATGCTTCGCC CTAACGTAAC CTGCTATATT GCAAATGGTG TGGTTTTATC ACCTCAGGCA TTACTTAGTG AAATTAAGGA GCTTGAGGGT AATGGCATCA ATGTGAGGGA GCGATTACGC ATTAGCCTGG CTTGCCCCCT GATTCTCCCT TACCACATTG CTTTGGATAA GGCTCGTGAG ACACATATGG GCAAGTCTGC AATTGGAACA ACAGGTCGTG GTATAGGTCC TGCTTATGAG GATAAAGTGG CTCGGCGCGC TTTAAGAGTA GGTGATTTAT TTCATAGAGA CCGATTTGCC AATAAATTAA CAGAATTATT GGATTATCAT AATTTTGTAT TAACACAATA TTTCAAACAA CCAGCTGTTG ATTTGGAATC CCTATTGGGT GAGTCATTAC AATGGGCTGA AGAATTAAGA CCAATGGTTT GCGATGTTTC TGCATGTTTG CATGAACATA GAAAACAAGG TGAAAATATT TTATTCGAAG GGGCGCAAGG TGTTTATTTA GATATTGATC ATGGAACATA CCCCTACGTA ACTTCTTCAA ATACCTGTGT GGGATCTGTA ATTAATGGTG CGGGATTTGG TCCTCGCTAT ATTGATTACG TGTTAGGCAT TACAAAAGCT TACACAACTC GAGTTGGCGG TGGCCCATTC CCCACTGAAT TACTTGATGA CGTTGGAAAA CGCATTGCGG AACGAGGACA GGAATTCGGC GCAGTAACTG GTAGACCCAG ACGTTGTGGG TGGTTTGACG CTGTTTTATT AAAACGCTCG ATTGAGTTAA ATAGTATTTC AGGACTCTGT GTTACCAAAT TGGATGTTCT CGATGGCTTA GAGGTATTAC GGATTGCTGT GGCTTATAAA GACAGGGATG GCAATATATT ATCTCGACCT CCTCTGGCAG CTGATGATTT CAACGATTTG TTACCAGTCT ATGAGGAGCT TCCTGGTTGG CAGGAATCTA CTGCTGATGT GACTGTGATG AGTGACTTAC CCGCCAATGC AAGGGCTTAT CTAAAGCGAA TTGAAGAGAT ATTAGGGATT CCTATTGATA TGCTATCAAC AGGACCTGAA AGGGATTCAA CAATTACTTT ACGAGGTCCT TTTCTTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T