LepnA.00882.a
Peptide deformylase
| CENTER ID: | LepnA.00882.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00882.a.B1.GE41436 | Full length(LepnA.00882.a) | 1 | 170 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6CAZ
DEPOSITED: 2/1/2018
DETERMINATION: XRay
CLONE: LepnA.00882.a.B1.GE41436
PROTEIN: LepnA.00882.a.B1.PW38390
6CK7
DEPOSITED: 2/27/2018
DETERMINATION: XRay
CLONE: LepnA.00882.a.B1.GE41436
PROTEIN: LepnA.00882.a.B1.PW38390
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.2767 |
| UniProt: | Q5ZSC4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIRKILYLP DERLRKIAKP VETFDESLQT LINDMFDTMY DARGVGLAAP QIGVSLRLSV IDIVGDKKEQ IVIVNPEIVS SHGEKEFEEG CLSVPGAYDT VVRAEKVTVK ALDRFGKPFE ITGEGLLAEC LQHEIDHMNG KLFVDMLSPL KRMMARRKLD KFKRLQARKP
NT Sequence
ATGGCAATTC GCAAGATTCT TTATTTACCT GATGAGCGGT TAAGAAAAAT AGCTAAGCCG GTCGAGACTT TTGATGAGAG TTTACAGACA TTAATCAACG ATATGTTTGA TACAATGTAT GATGCTCGGG GAGTTGGGCT TGCCGCACCA CAAATAGGAG TCAGCTTGCG TTTATCTGTC ATTGATATAG TGGGAGATAA AAAGGAGCAG ATCGTTATTG TTAATCCTGA AATAGTTTCC TCTCATGGCG AAAAAGAATT TGAAGAAGGC TGTTTGTCTG TCCCAGGGGC TTATGATACC GTTGTCAGAG CTGAAAAAGT GACCGTGAAG GCACTGGACA GGTTTGGCAA ACCGTTTGAA ATTACGGGTG AAGGGCTGCT TGCCGAGTGT TTACAACATG AAATTGACCA TATGAATGGC AAATTGTTTG TCGATATGTT GTCTCCATTG AAGAGAATGA TGGCTCGCCG GAAATTAGAT AAGTTCAAGC GTTTACAAGC TCGTAAGCCA
Details for LepnA.00882.a.B1.GE41436
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 22kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA IRKILYLPDE RLRKIAKPVE TFDESLQTLI NDMFDTMYDA RGVGLAAPQI GVSLRLSVID IVGDKKEQIV IVNPEIVSSH GEKEFEEGCL SVPGAYDTVV RAEKVTVKAL DRFGKPFEIT GEGLLAECLQ HEIDHMNGKL FVDMLSPLKR MMARRKLDKF KRLQARKP
Validated NT Sequence
atggctcacc accaccacca ccatatggca attcgcaaga ttctttattt acctgatgag cggttaagaa aaatagctaa gccggtcgag acttttgatg agagtttaca gacattaatc aacgatatgt ttgatacaat gtatgatgct cggggagttg ggcttgccgc accacaaata ggagtcagct tgcgtttatc tgtcattgat atagtgggag ataaaaagga gcagatcgtt attgttaatc ctgaaatagt ttcctctcat ggcgaaaaag aatttgaaga aggctgtttg tctgtcccag gggcttatga taccgttgtc agagctgaaa aagtgaccgt gaaggcactg gacaggtttg gcaaaccgtt tgaaattacg ggtgaagggc tgcttgccga gtgtttacaa catgaaattg accatatgaa tggcaaattg tttgtcgata tgttgtctcc attgaagaga atgatggctc gccggaaatt agataagttc aagcgtttac aagctcgtaa gccataagtg agtaagat
Expected Protein Sequence
MAHHHHHHMA IRKILYLPDE RLRKIAKPVE TFDESLQTLI NDMFDTMYDA RGVGLAAPQI GVSLRLSVID IVGDKKEQIV IVNPEIVSSH GEKEFEEGCL SVPGAYDTVV RAEKVTVKAL DRFGKPFEIT GEGLLAECLQ HEIDHMNGKL FVDMLSPLKR MMARRKLDKF KRLQARKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa ttcgcaagat tctttattta cctgatgagc ggttaagaaa aatagctaag ccggtcgaga cttttgatga gagtttacag acattaatca acgatatgtt tgatacaatg tatgatgctc ggggagttgg gcttgccgca ccacaaatag gagtcagctt gcgtttatct gtcattgata tagtgggaga taaaaaggag cagatcgtta ttgttaatcc tgaaatagtt tcctctcatg gcgaaaaaga atttgaagaa ggctgtttgt ctgtcccagg ggcttatgat accgttgtca gagctgaaaa agtgaccgtg aaggcactgg acaggtttgg caaaccgttt gaaattacgg gtgaagggct gcttgccgag tgtttacaac atgaaattga ccatatgaat ggcaaattgt ttgtcgatat gttgtctcca ttgaagagaa tgatggctcg ccggaaatta gataagttca agcgtttaca agctcgtaag ccatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat