SSGCID: Target Page

LepnA.00663.a

Cytidylate kinase

CENTER ID:	LepnA.00663.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.00663.a.B1.GE41431	Full length(LepnA.00663.a)		1	231

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272624.6.peg.1490
UniProt:	Q5ZVM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMVFNNEVPV ITLDGPSGTG KGTICHLVAK RLHWNMLDSG AIYRVLAYAA RKNSIEPNDI KKLTALARSL NLRFESSADN ETKVILDDEN VSQQIRSEQC GQDASQIAVI PEVRAALLER QRNFAQFPGL VTDGRDMGTV VFPNAVLKVY LYASAEERAN RRYLQLQDNG INVSLAQVVE ELAKRDARDT ARTHAPLKPA EDAVLIDTTG LTIVQVFNIV LKLIDERLNN L

NT Sequence

CTGATGGTTT TTAATAATGA GGTTCCTGTC ATTACGTTGG ATGGTCCAAG TGGAACAGGA AAGGGGACAA TTTGTCATTT GGTAGCTAAG AGACTGCATT GGAATATGCT TGACAGTGGT GCTATTTATC GTGTTCTAGC TTATGCTGCT CGGAAAAATA GCATCGAACC GAATGATATT AAAAAATTGA CTGCTTTGGC CCGTTCCCTG AATTTGCGTT TTGAATCATC TGCGGATAAT GAAACAAAAG TAATACTTGA TGATGAAAAT GTCAGCCAGC AAATCCGCAG TGAGCAATGT GGTCAGGATG CCTCACAAAT TGCAGTGATT CCTGAAGTAA GAGCAGCACT GCTTGAACGC CAGAGAAACT TTGCCCAATT TCCTGGATTG GTGACCGATG GACGCGATAT GGGGACGGTG GTTTTTCCAA ATGCTGTTTT GAAGGTTTAT TTATATGCAA GTGCAGAAGA AAGAGCAAAT AGACGATATT TACAGTTGCA AGATAATGGA ATTAATGTTA GCCTCGCCCA GGTTGTTGAA GAACTGGCTA AACGGGATGC AAGGGATACA GCTCGTACGC ATGCGCCATT GAAACCAGCT GAAGATGCTG TCTTGATCGA TACAACCGGA CTAACAATTG TACAAGTGTT CAATATTGTA TTAAAATTAA TTGATGAACG TTTGAATAAT TTG

Details for LepnA.00663.a.B1.GE41431

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	27kDa
OBSERVED MW:	14kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML MVFNNEVPVI TLDGPSGTGK GTICHLVAKR LHWNMLDSGA IYRVLAYAAR KNSIEPNDIK KLTALARSLN LRFESSADNE TKVILDDENV SQQIRSEQCG QDASQIAVIP EVRAALLERQ RNFAQFPGLV TDGRDMGTVV FPNAVLKVYL YASAEERANR RYLQLQDNGI NVSLAQVVEE LAKRDARDTA RTHAPLKPAE DAVLIDTTGL TIVQVFNIVL KLIDERLNNL

Validated NT Sequence

atggctcacc accaccacca ccatatgctg atggttttta ataatgaggt tcctgtcatt acgttggatg gtccaagtgg aacaggaaag gggacaattt gtcatttggt agctaagaga ctgcattgga atatgcttga cagtggtgct atttatcgtg ttctagctta tgctgctcgg aaaaatagca tcgaaccgaa tgatattaaa aaattgactg ctttggcccg ttccctgaat ttgcgttttg aatcatctgc ggataatgaa acaaaagtaa tacttgatga tgaaaatgtc agccagcaaa tccgcagtga gcaatgtggt caggatgcct cacaaattgc agtgattcct gaagtaagag cagcactgct tgaacgccag agaaactttg cccaatttcc tggattggtg accgatggac gcgatatggg gacggtggtt tttccaaatg ctgttttgaa ggtttattta tatgcaagtg cagaagaaag agcaaataga cgatatttac agttgcaaga taatggaatt aatgttagcc tcgcccaggt tgttgaagaa ctggctaaac gggatgcaag ggatacagct cgtacgcatg cgccattgaa accagctgaa gatgctgtct tgatcgatac aaccggacta acaattgtac aagtgttcaa tattgtatta aaattaattg atgaacgttt gaataatttg taagtgagta agat

Expected Protein Sequence

MAHHHHHHMM VFNNEVPVIT LDGPSGTGKG TICHLVAKRL HWNMLDSGAI YRVLAYAARK NSIEPNDIKK LTALARSLNL RFESSADNET KVILDDENVS QQIRSEQCGQ DASQIAVIPE VRAALLERQR NFAQFPGLVT DGRDMGTVVF PNAVLKVYLY ASAEERANRR YLQLQDNGIN VSLAQVVEEL AKRDARDTAR THAPLKPAED AVLIDTTGLT IVQVFNIVLK LIDERLNNL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgatgg tttttaataa tgaggttcct gtcattacgt tggatggtcc aagtggaaca ggaaagggga caatttgtca tttggtagct aagagactgc attggaatat gcttgacagt ggtgctattt atcgtgttct agcttatgct gctcggaaaa atagcatcga accgaatgat attaaaaaat tgactgcttt ggcccgttcc ctgaatttgc gttttgaatc atctgcggat aatgaaacaa aagtaatact tgatgatgaa aatgtcagcc agcaaatccg cagtgagcaa tgtggtcagg atgcctcaca aattgcagtg attcctgaag taagagcagc actgcttgaa cgccagagaa actttgccca atttcctgga ttggtgaccg atggacgcga tatggggacg gtggtttttc caaatgctgt tttgaaggtt tatttatatg caagtgcaga agaaagagca aatagacgat atttacagtt gcaagataat ggaattaatg ttagcctcgc ccaggttgtt gaagaactgg ctaaacggga tgcaagggat acagctcgta cgcatgcgcc attgaaacca gctgaagatg ctgtcttgat cgatacaacc ggactaacaa ttgtacaagt gttcaatatt gtattaaaat taattgatga acgtttgaat aatttgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t