LepnA.00629.a
Acetyl-coenzyme A synthetase
| CENTER ID: | LepnA.00629.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00629.a.B1.GE41429 | Full length(LepnA.00629.a) | 1 | 627 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00629.a.B1.PW38314 | Full length(LepnA.00629.a) | 1 | 627 |
Structures
7MMZ
DEPOSITED: 4/30/2021
DETERMINATION: XRay
CLONE: LepnA.00629.a.B1.GE41429
PROTEIN: LepnA.00629.a.B1.PW38314
Publications by SSGCID
Bacterial Structural Genomics Target Enabled by Recently Discovered Potent Fungal ACS Inhibitor
Abendroth J, Hagen TJ, Bolejack M, Esan TE, DeBouver ND, Krysan DJ
Acta Crystallogr F Struct Biol Commun - 2023
issue 79, pages 137-143
PMID: 37223974; PMCID: PMC10231259
PDB:
7MMZ
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.133 |
| UniProt: | Q5ZZ84 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPVHLKHPH LTATEEYLSE FWSEIAAQTI DWIQPWSITL QGGLHKGDVK WFQGGLLNVS ANCLDRHLPH KANQTAIIWE GDDENQNKTL TFAQLYSEVC KMSNVLKSLN VRRGDTVGIY LPMIPEAAIA MLACARIGAI HTVVFAGFSA HALQQRLIAS SCKCLITADA FQRGGKTIPL KKQADEASVD LNITKLVVKN SNAPTALNKN KEHWWHELKQ TVSDQCTPEP MNTEDPLFIL YTSGSTGQPK GVVHTTGGYL VQAAYTHQLI FACQDNEVFW CTADVGWITG HSYVVYGPLC NGITTLMFEG IPTWPDAARN WRIIDKHQVN VFYTAPTAIR SLMRAGDQWL NSSSRSSLRL LGSVGEPINP EAWNWYHQKV GQGKCPIVDT WWQTETGAIM ISPRASDEVI KPGSARKPIP GIVPLLLNEQ GHEINGAGEG LLAIKYPWPS MARTIAGDHQ RYCNTYLSNG YYITGDGAKR DEDGDYWITG RIDDVLNVSG HRLGTAEIES ALVSHPKVAE AGVVGIPHDL KGQAIFAYVI LKQGNKPDAE LQTELMERVK DQISAIAKPD VIQFANDLPK TRSGKIMRRI LRKIACKEVS HIDELGDLTT LANPQIVEEL MKNILKR
NT Sequence
ATGCTTCCCG TTCATCTAAA GCACCCTCAT TTAACAGCTA CAGAAGAATA TCTATCTGAA TTTTGGTCTG AAATTGCCGC ACAAACCATT GACTGGATAC AACCTTGGAG TATCACCCTG CAGGGAGGAT TACACAAAGG CGATGTGAAG TGGTTTCAAG GCGGCTTACT CAATGTTAGC GCTAATTGTC TGGATAGGCA TTTACCCCAT AAAGCAAATC AAACAGCCAT CATTTGGGAA GGTGATGATG AAAACCAAAA TAAAACCCTG ACTTTTGCGC AGCTTTATTC TGAAGTATGC AAAATGAGCA ATGTGTTAAA AAGCCTAAAT GTTCGCAGAG GGGATACGGT AGGCATTTAC CTACCCATGA TTCCTGAAGC GGCCATTGCC ATGCTGGCTT GTGCTCGTAT AGGAGCTATT CATACCGTTG TGTTTGCCGG GTTTTCGGCC CATGCATTGC AGCAGCGATT AATAGCCTCT TCCTGTAAAT GTTTAATCAC TGCGGATGCG TTTCAACGCG GTGGAAAAAC TATCCCTTTA AAGAAACAGG CTGACGAAGC CAGCGTGGAC TTAAATATCA CCAAGCTGGT TGTTAAAAAT TCAAATGCCC CAACGGCACT AAATAAGAAC AAAGAACATT GGTGGCATGA GCTAAAACAA ACCGTATCGG ATCAATGCAC GCCGGAACCA ATGAACACCG AAGATCCCCT GTTTATTCTC TATACTTCCG GAAGCACGGG ACAGCCGAAA GGAGTGGTAC ATACCACAGG CGGCTATTTA GTGCAGGCCG CTTATACGCA TCAACTGATA TTTGCTTGTC AGGATAATGA AGTATTTTGG TGTACTGCTG ATGTCGGTTG GATTACAGGG CATAGTTATG TCGTCTATGG CCCTCTCTGC AATGGCATTA CCACATTGAT GTTTGAAGGA ATACCCACGT GGCCTGACGC AGCAAGAAAC TGGCGCATCA TTGATAAGCA CCAAGTGAAT GTGTTCTACA CAGCTCCCAC AGCCATCCGC TCCCTGATGA GAGCTGGCGA TCAATGGCTA AACTCAAGCT CAAGAAGTTC TCTTCGTTTA CTGGGTTCAG TGGGTGAGCC AATTAACCCG GAAGCATGGA ATTGGTATCA CCAAAAAGTT GGACAAGGGA AATGCCCGAT AGTGGATACA TGGTGGCAAA CTGAAACCGG AGCTATTATG ATTAGTCCCA GAGCCAGTGA TGAAGTGATC AAACCAGGCT CTGCTCGTAA ACCTATTCCG GGGATCGTAC CACTCCTGTT AAATGAGCAA GGACATGAAA TTAATGGCGC TGGAGAAGGC CTTTTGGCTA TCAAATACCC ATGGCCTTCT ATGGCAAGAA CCATTGCAGG AGATCACCAA AGATACTGTA ACACTTATTT ATCCAACGGC TATTACATCA CTGGTGATGG AGCCAAACGA GATGAAGATG GAGATTATTG GATTACAGGC CGAATTGATG ACGTACTTAA CGTTTCCGGC CATCGATTGG GTACTGCTGA AATTGAATCA GCATTGGTCA GCCATCCTAA GGTTGCTGAA GCTGGCGTTG TAGGTATTCC TCATGATTTA AAAGGTCAAG CAATATTTGC CTATGTCATT CTGAAGCAGG GAAATAAGCC TGATGCCGAG CTCCAAACTG AATTGATGGA GCGAGTCAAA GATCAAATCA GTGCTATCGC CAAACCAGAT GTCATTCAGT TTGCAAATGA TTTGCCGAAA ACCCGTTCAG GTAAAATTAT GCGCCGAATA CTAAGAAAAA TTGCTTGTAA AGAAGTGTCG CACATTGATG AGCTGGGTGA TTTAACAACT CTTGCTAACC CTCAGATCGT TGAGGAACTC ATGAAAAATA TTTTGAAGCG G
Details for LepnA.00629.a.B1.GE41429
| HARVESTED ON: | 4/5/2017 |
| SEQUENCED ON: | 4/27/2017 |
| EXPECTED MW: | 71kDa |
| OBSERVED MW: | 71kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML PVHLKHPHLT ATEEYLSEFW SEIAAQTIDW IQPWSITLQG GLHKGDVKWF QGGLLNVSAN CLDRHLPHKA NQTAIIWEGD DENQNKTLTF AQLYSEVCKM SNVLKSLNVR RGDTVGIYLP MIPEAAIAML ACARIGAIHT VVFAGFSAHA LQQRLIASSC KCLITADAFQ RGGKTIPLKK QADEASVDLN ITKLVVKNSN APTALNKNKE HWWHELKQTV SDQCTPEPMN TEDPLFILYT SGSTGQPKGV VHTTGGYLVQ AAYTHQLIFA CQDNEVFWCT ADVGWITGHS YVVYGPLCNG ITTLMFEGIP TWPDAARNWR IIDKHQVNVF YTAPTAIRSL MRAGDQWLNS SSRSSLRLLG SVGEPINPEA WNWYHQKVGQ GKCPIVDTWW QTETGAIMIS PRASDEVIKP GSARKPIPGI VPLLLNEQGH EINGAGEGLL AIKYPWPSMA RTIAGDHQRY CNTYLSNGYY ITGDGAKRDE DGDYWITGRI DDVLNVSGHR LGTAEIESAL VSHPKVAEAG VVGIPHDLKG QAIFAYVILK QGNKPDAELQ TELMERVKDQ ISAIAKPDVI QFANDLPKTR SGKIMRRILR KIACKEVSHI DELGDLTTLA NPQIVEELMK NILKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc ccgttcatct aaagcaccct catttaacag ctacagaaga atatctatct gaattttggt ctgaaattgc cgcacaaacc attgactgga tacaaccttg gagtatcacc ctgcagggag gattacacaa aggcgatgtg aagtggtttc aaggcggctt actcaatgtt agcgctaatt gtctggatag gcatttaccc cataaagcaa atcaaacagc catcatttgg gaaggtgatg atgaaaacca aaataaaacc ctgacttttg cgcagcttta ttctgaagta tgcaaaatga gcaatgtgtt aaaaagccta aatgttcgca gaggggatac ggtaggcatt tacctaccca tgattcctga agcggccatt gccatgctgg cttgtgctcg tataggagct attcataccg ttgtgtttgc cgggttttcg gcccatgcat tgcagcagcg attaatagcc tcttcctgta aatgtttaat cactgcggat gcgtttcaac gcggtggaaa aactatccct ttaaagaaac aggctgacga agccagcgtg gacttaaata tcaccaagct ggttgttaaa aattcaaatg ccccaacggc actaaataag aacaaagaac attggtggca tgagctaaaa caaaccgtat cggatcaatg cacgccggaa ccaatgaaca ccgaagatcc cctgtttatt ctctatactt ccggaagcac gggacagccg aaaggagtgg tacataccac aggcggctat ttagtgcagg ccgcttatac gcatcaactg atatttgctt gtcaggataa tgaagtattt tggtgtactg ctgatgtcgg ttggattaca gggcatagtt atgtcgtcta tggccctctc tgcaatggca ttaccacatt gatgtttgaa ggaataccca cgtggcctga cgcagcaaga aactggcgca tcattgataa gcaccaagtg aatgtgttct acacagctcc cacagccatc cgctccctga tgagagctgg cgatcaatgg ctaaactcaa gctcaagaag ttctcttcgt ttactgggtt cagtgggtga gccaattaac ccggaagcat ggaattggta tcaccaaaaa gttggacaag ggaaatgccc gatagtggat acatggtggc aaactgaaac cggagctatt atgattagtc ccagagccag tgatgaagtg atcaaaccag gctctgctcg taaacctatt ccggggatcg taccactcct gttaaatgag caaggacatg aaattaatgg cgctggagaa ggccttttgg ctatcaaata cccatggcct tctatggcaa gaaccattgc aggagatcac caaagatact gtaacactta tttatccaac ggctattaca tcactggtga tggagccaaa cgagatgaag atggagatta ttggattaca ggccgaattg atgacgtact taacgtttcc ggccatcgat tgggtactgc tgaaattgaa tcagcattgg tcagccatcc taaggttgct gaagctggcg ttgtaggtat tcctcatgat ttaaaaggtc aagcaatatt tgcctatgtc attctgaagc agggaaataa gcctgatgcc gagctccaaa ctgaattgat ggagcgagtc aaagatcaaa tcagtgctat cgccaaacca gatgtcattc agtttgcaaa tgatttgccg aaaacccgtt caggtaaaat tatgcgccga atactaagaa aaattgcttg taaagaagtg tcgcacattg atgagctggg tgatttaaca actcttgcta accctcagat cgttgaggaa ctcatgaaaa atattttgaa gcggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LepnA.00629.a.B1.PW38314
| PURIFICATION DATe: | 7/17/2017 |
| CONCENTRATION: | 33.45mg/ml |
| OBSERVED MW: | 69kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHML PVHLKHPHLT ATEEYLSEFW SEIAAQTIDW IQPWSITLQG GLHKGDVKWF QGGLLNVSAN CLDRHLPHKA NQTAIIWEGD DENQNKTLTF AQLYSEVCKM SNVLKSLNVR RGDTVGIYLP MIPEAAIAML ACARIGAIHT VVFAGFSAHA LQQRLIASSC KCLITADAFQ RGGKTIPLKK QADEASVDLN ITKLVVKNSN APTALNKNKE HWWHELKQTV SDQCTPEPMN TEDPLFILYT SGSTGQPKGV VHTTGGYLVQ AAYTHQLIFA CQDNEVFWCT ADVGWITGHS YVVYGPLCNG ITTLMFEGIP TWPDAARNWR IIDKHQVNVF YTAPTAIRSL MRAGDQWLNS SSRSSLRLLG SVGEPINPEA WNWYHQKVGQ GKCPIVDTWW QTETGAIMIS PRASDEVIKP GSARKPIPGI VPLLLNEQGH EINGAGEGLL AIKYPWPSMA RTIAGDHQRY CNTYLSNGYY ITGDGAKRDE DGDYWITGRI DDVLNVSGHR LGTAEIESAL VSHPKVAEAG VVGIPHDLKG QAIFAYVILK QGNKPDAELQ TELMERVKDQ ISAIAKPDVI QFANDLPKTR SGKIMRRILR KIACKEVSHI DELGDLTTLA NPQIVEELMK NILKR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTC CCGTTCATCT AAAGCACCCT CATTTAACAG CTACAGAAGA ATATCTATCT GAATTTTGGT CTGAAATTGC CGCACAAACC ATTGACTGGA TACAACCTTG GAGTATCACC CTGCAGGGAG GATTACACAA AGGCGATGTG AAGTGGTTTC AAGGCGGCTT ACTCAATGTT AGCGCTAATT GTCTGGATAG GCATTTACCC CATAAAGCAA ATCAAACAGC CATCATTTGG GAAGGTGATG ATGAAAACCA AAATAAAACC CTGACTTTTG CGCAGCTTTA TTCTGAAGTA TGCAAAATGA GCAATGTGTT AAAAAGCCTA AATGTTCGCA GAGGGGATAC GGTAGGCATT TACCTACCCA TGATTCCTGA AGCGGCCATT GCCATGCTGG CTTGTGCTCG TATAGGAGCT ATTCATACCG TTGTGTTTGC CGGGTTTTCG GCCCATGCAT TGCAGCAGCG ATTAATAGCC TCTTCCTGTA AATGTTTAAT CACTGCGGAT GCGTTTCAAC GCGGTGGAAA AACTATCCCT TTAAAGAAAC AGGCTGACGA AGCCAGCGTG GACTTAAATA TCACCAAGCT GGTTGTTAAA AATTCAAATG CCCCAACGGC ACTAAATAAG AACAAAGAAC ATTGGTGGCA TGAGCTAAAA CAAACCGTAT CGGATCAATG CACGCCGGAA CCAATGAACA CCGAAGATCC CCTGTTTATT CTCTATACTT CCGGAAGCAC GGGACAGCCG AAAGGAGTGG TACATACCAC AGGCGGCTAT TTAGTGCAGG CCGCTTATAC GCATCAACTG ATATTTGCTT GTCAGGATAA TGAAGTATTT TGGTGTACTG CTGATGTCGG TTGGATTACA GGGCATAGTT ATGTCGTCTA TGGCCCTCTC TGCAATGGCA TTACCACATT GATGTTTGAA GGAATACCCA CGTGGCCTGA CGCAGCAAGA AACTGGCGCA TCATTGATAA GCACCAAGTG AATGTGTTCT ACACAGCTCC CACAGCCATC CGCTCCCTGA TGAGAGCTGG CGATCAATGG CTAAACTCAA GCTCAAGAAG TTCTCTTCGT TTACTGGGTT CAGTGGGTGA GCCAATTAAC CCGGAAGCAT GGAATTGGTA TCACCAAAAA GTTGGACAAG GGAAATGCCC GATAGTGGAT ACATGGTGGC AAACTGAAAC CGGAGCTATT ATGATTAGTC CCAGAGCCAG TGATGAAGTG ATCAAACCAG GCTCTGCTCG TAAACCTATT CCGGGGATCG TACCACTCCT GTTAAATGAG CAAGGACATG AAATTAATGG CGCTGGAGAA GGCCTTTTGG CTATCAAATA CCCATGGCCT TCTATGGCAA GAACCATTGC AGGAGATCAC CAAAGATACT GTAACACTTA TTTATCCAAC GGCTATTACA TCACTGGTGA TGGAGCCAAA CGAGATGAAG ATGGAGATTA TTGGATTACA GGCCGAATTG ATGACGTACT TAACGTTTCC GGCCATCGAT TGGGTACTGC TGAAATTGAA TCAGCATTGG TCAGCCATCC TAAGGTTGCT GAAGCTGGCG TTGTAGGTAT TCCTCATGAT TTAAAAGGTC AAGCAATATT TGCCTATGTC ATTCTGAAGC AGGGAAATAA GCCTGATGCC GAGCTCCAAA CTGAATTGAT GGAGCGAGTC AAAGATCAAA TCAGTGCTAT CGCCAAACCA GATGTCATTC AGTTTGCAAA TGATTTGCCG AAAACCCGTT CAGGTAAAAT TATGCGCCGA ATACTAAGAA AAATTGCTTG TAAAGAAGTG TCGCACATTG ATGAGCTGGG TGATTTAACA ACTCTTGCTA ACCCTCAGAT CGTTGAGGAA CTCATGAAAA ATATTTTGAA GCGGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT