LepnA.00398.a

ATP-dependent Clp protease proteolytic subunit

CENTER ID:	LepnA.00398.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.00398.a.B1.GE41427	Full length(LepnA.00398.a)		1	214

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272624.6.peg.1950
UniProt:	Q5ZUD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPGYSDNIIR NASGLIPMVI EQTSRGERSY DIYSRLLKER IIFLLGEVED HMANLVVAQL LFLESENPEK DISLYINSPG GVVTAGLAIY DTMQFIKPDV STLCIGQAAS AAALLLCAGA EGKRFCLPNS RVMIHQPLGG YRGQATDIEI HARETLAVRE RLNNIMAKHT KKTPDQIMRD TERDNFMSAT QAMEYGLIDK VLYDRQVAGH STDL

NT Sequence

ATGCCAGGCT ATTCAGATAA TATTATCCGT AATGCAAGCG GATTAATACC AATGGTCATT GAGCAAACCT CGCGAGGTGA GCGTTCCTAT GACATTTACT CAAGATTATT AAAAGAAAGA ATTATTTTTC TCTTGGGTGA AGTAGAAGAT CATATGGCTA ACCTGGTTGT GGCTCAGTTG TTGTTTCTGG AGTCTGAAAA TCCAGAGAAA GATATATCTC TATATATTAA TTCTCCCGGC GGAGTCGTTA CTGCAGGTCT TGCTATCTAC GACACCATGC AATTTATTAA GCCAGATGTA AGTACATTAT GCATAGGCCA GGCAGCAAGT GCAGCTGCCT TGCTACTTTG TGCCGGTGCT GAAGGCAAAC GATTCTGCTT ACCTAACTCA AGAGTTATGA TACATCAGCC CTTGGGTGGC TATAGAGGTC AAGCAACAGA CATAGAAATT CATGCTCGAG AGACCTTGGC AGTTAGGGAA CGTTTGAATA ATATTATGGC TAAACACACT AAAAAAACAC CTGATCAAAT CATGCGTGAT ACAGAAAGAG ATAACTTTAT GAGTGCTACT CAGGCTATGG AGTATGGTCT AATCGATAAA GTTCTCTATG ACAGACAAGT TGCTGGACAT TCTACAGACT TA

Details for LepnA.00398.a.B1.GE41427

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/27/2017
EXPECTED MW:	25kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP GYSDNIIRNA SGLIPMVIEQ TSRGERSYDI YSRLLKERII FLLGEVEDHM ANLVVAQLLF LESENPEKDI SLYINSPGGV VTAGLAIYDT MQFIKPDVST LCIGQAASAA ALLLCAGAEG KRFCLPNSRV MIHQPLGGYR GQATDIEIHA RETLAVRERL NNIMAKHTKK TPDQIMRDTE RDNFMSATQA MEYGLIDKVL YDRQVAGHST DL

Validated NT Sequence

atggctcacc accaccacca ccatatgcca ggctattcag ataatattat ccgtaatgca agcggattaa taccaatggt cattgagcaa acctcgcgag gtgagcgttc ctatgacatt tactcaagat tattaaaaga aagaattatt tttctcttgg gtgaagtaga agatcatatg gctaacctgg ttgtggctca gttgttgttt ctggagtctg aaaatccaga gaaagatata tctctatata ttaattctcc cggcggagtc gttactgcag gtcttgctat ctacgacacc atgcaattta ttaagccaga tgtaagtaca ttatgcatag gccaggcagc aagtgcagct gccttgctac tttgtgccgg tgctgaaggc aaacgattct gcttacctaa ctcaagagtt atgatacatc agcccttggg tggctataga ggtcaagcaa cagacataga aattcatgct cgagagacct tggcagttag ggaacgtttg aataatatta tggctaaaca cactaaaaaa acacctgatc aaatcatgcg tgatacagaa agagataact ttatgagtgc tactcaggct atggagtatg gtctaatcga taaagttctc tatgacagac aagttgctgg acattctaca gacttataag tgagtaagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag gctattcaga taatattatc cgtaatgcaa gcggattaat accaatggtc attgagcaaa cctcgcgagg tgagcgttcc tatgacattt actcaagatt attaaaagaa agaattattt ttctcttggg tgaagtagaa gatcatatgg ctaacctggt tgtggctcag ttgttgtttc tggagtctga aaatccagag aaagatatat ctctatatat taattctccc ggcggagtcg ttactgcagg tcttgctatc tacgacacca tgcaatttat taagccagat gtaagtacat tatgcatagg ccaggcagca agtgcagctg ccttgctact ttgtgccggt gctgaaggca aacgattctg cttacctaac tcaagagtta tgatacatca gcccttgggt ggctatagag gtcaagcaac agacatagaa attcatgctc gagagacctt ggcagttagg gaacgtttga ataatattat ggctaaacac actaaaaaaa cacctgatca aatcatgcgt gatacagaaa gagataactt tatgagtgct actcaggcta tggagtatgg tctaatcgat aaagttctct atgacagaca agttgctgga cattctacag acttatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat