LepnA.00364.a

Pyridoxine 5'-phosphate synthase

CENTER ID: LepnA.00364.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00364.a.B1.GE41426 Full length(LepnA.00364.a) 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00364.a.B1.PS38360 Full length(LepnA.00364.a) 1 248

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|272624.6.peg.979
UniProt: Q3V870

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKELLLGVN IDHIATIRQA RGTRYPDPVQ AAMDAEEAGA DGITLHMRED LRHIQARDVR LIKQVLQTRM NLELAVTEAM LDFAEEISPE HACLVPEKRE ELTTEGGLDI LTHRNVVEKA VRRLQLMGSE VSLFIDPDKE QIRAAVDVGA PVIELHTGCY ADATSEEKQH YELQRIKEAA EFAASLNLVV NAGHGLHYHN VKPIAAIREL NELNIGHAII ARALFCGLKE AVRHMRQLMQ EARLYVND
NT Sequence
ATGAATAAAG AGTTATTGTT AGGTGTTAAT ATTGATCATA TAGCAACCAT AAGACAAGCA CGAGGAACTC GTTATCCTGA TCCTGTCCAG GCAGCAATGG ATGCTGAAGA AGCAGGGGCA GATGGAATTA CTTTGCATAT GAGAGAGGAC TTACGACATA TTCAGGCGCG TGATGTCAGA TTGATTAAAC AGGTTCTGCA AACACGTATG AATCTTGAGC TGGCGGTTAC TGAAGCCATG CTTGATTTTG CCGAGGAAAT ATCGCCGGAG CATGCTTGCT TAGTGCCTGA AAAGCGGGAA GAACTAACTA CAGAAGGTGG ATTGGATATT TTGACTCACC GAAATGTGGT GGAAAAGGCA GTACGTCGCT TACAATTAAT GGGAAGTGAG GTTTCTTTAT TTATTGACCC GGATAAAGAG CAAATAAGAG CTGCGGTTGA TGTTGGGGCG CCAGTTATCG AATTGCACAC TGGGTGTTAT GCTGATGCCA CCTCAGAGGA AAAGCAGCAT TATGAATTGC AACGAATTAA AGAGGCAGCT GAATTTGCTG CCAGTTTAAA CTTGGTAGTG AATGCCGGTC ATGGGTTGCA CTATCATAAT GTTAAACCGA TTGCAGCGAT TAGGGAGTTA AATGAACTTA ACATAGGACA TGCCATTATT GCCAGAGCAT TATTCTGTGG ATTAAAAGAG GCAGTAAGAC ACATGCGCCA ATTAATGCAG GAAGCGAGAC TTTATGTCAA CGAC
Details for LepnA.00364.a.B1.GE41426
HARVESTED ON: 4/5/2017
SEQUENCED ON: 4/27/2017
EXPECTED MW: 29kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN KELLLGVNID HIATIRQARG TRYPDPVQAA MDAEEAGADG ITLHMREDLR HIQARDVRLI KQVLQTRMNL ELAVTEAMLD FAEEISPEHA CLVPEKREEL TTEGGLDILT HRNVVEKAVR RLQLMGSEVS LFIDPDKEQI RAAVDVGAPV IELHTGCYAD ATSEEKQHYE LQRIKEAAEF AASLNLVVNA GHGLHYHNVK PIAAIRELNE LNIGHAIIAR ALFCGLKEAV RHMRQLMQEX XLYVND
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aaagagttat tgttaggtgt taatattgat catatagcaa ccataagaca agcacgagga actcgttatc ctgatcctgt ccaggcagca atggatgctg aagaagcagg ggcagatgga attactttgc atatgagaga ggacttacga catattcagg cgcgtgatgt cagattgatt aaacaggttc tgcaaacacg tatgaatctt gagctggcgg ttactgaagc catgcttgat tttgccgagg aaatatcgcc ggagcatgct tgcttagtgc ctgaaaagcg ggaagaacta actacagaag gtggattgga tattttgact caccgaaatg tggtggaaaa ggcagtacgt cgcttacaat taatgggaag tgaggtttct ttatttattg acccggataa agagcaaata agagctgcgg ttgatgttgg ggcgccagtt atcgaattgc acactgggtg ttatgctgat gccacctcag aggaaaagca gcattatgaa ttgcaacgaa ttaaagaggc agctgaattt gctgccagtt taaacttggt agtgaatgcc ggtcatgggt tgcactatca taatgttaaa ccgattgcag cgattaggga gttaaatgaa cttaacatag gacatgccat tattgccaga gcattattct gtggattaaa agaggcagta agacacatgc gccaattaat gcaggaagnn ngactttatg tcaacgacta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMN KELLLGVNID HIATIRQARG TRYPDPVQAA MDAEEAGADG ITLHMREDLR HIQARDVRLI KQVLQTRMNL ELAVTEAMLD FAEEISPEHA CLVPEKREEL TTEGGLDILT HRNVVEKAVR RLQLMGSEVS LFIDPDKEQI RAAVDVGAPV IELHTGCYAD ATSEEKQHYE LQRIKEAAEF AASLNLVVNA GHGLHYHNVK PIAAIRELNE LNIGHAIIAR ALFCGLKEAV RHMRQLMQEA RLYVND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aagagttatt gttaggtgtt aatattgatc atatagcaac cataagacaa gcacgaggaa ctcgttatcc tgatcctgtc caggcagcaa tggatgctga agaagcaggg gcagatggaa ttactttgca tatgagagag gacttacgac atattcaggc gcgtgatgtc agattgatta aacaggttct gcaaacacgt atgaatcttg agctggcggt tactgaagcc atgcttgatt ttgccgagga aatatcgccg gagcatgctt gcttagtgcc tgaaaagcgg gaagaactaa ctacagaagg tggattggat attttgactc accgaaatgt ggtggaaaag gcagtacgtc gcttacaatt aatgggaagt gaggtttctt tatttattga cccggataaa gagcaaataa gagctgcggt tgatgttggg gcgccagtta tcgaattgca cactgggtgt tatgctgatg ccacctcaga ggaaaagcag cattatgaat tgcaacgaat taaagaggca gctgaatttg ctgccagttt aaacttggta gtgaatgccg gtcatgggtt gcactatcat aatgttaaac cgattgcagc gattagggag ttaaatgaac ttaacatagg acatgccatt attgccagag cattattctg tggattaaaa gaggcagtaa gacacatgcg ccaattaatg caggaagcga gactttatgt caacgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LepnA.00364.a.B1.PS38360
PURIFICATION DATe: 10/11/2017
CONCENTRATION: 44.6mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN KELLLGVNID HIATIRQARG TRYPDPVQAA MDAEEAGADG ITLHMREDLR HIQARDVRLI KQVLQTRMNL ELAVTEAMLD FAEEISPEHA CLVPEKREEL TTEGGLDILT HRNVVEKAVR RLQLMGSEVS LFIDPDKEQI RAAVDVGAPV IELHTGCYAD ATSEEKQHYE LQRIKEAAEF AASLNLVVNA GHGLHYHNVK PIAAIRELNE LNIGHAIIAR ALFCGLKEAV RHMRQLMQEX XLYVND
Validated NT Sequence
atggctcacc accaccacca ccatatgaat aaagagttat tgttaggtgt taatattgat catatagcaa ccataagaca agcacgagga actcgttatc ctgatcctgt ccaggcagca atggatgctg aagaagcagg ggcagatgga attactttgc atatgagaga ggacttacga catattcagg cgcgtgatgt cagattgatt aaacaggttc tgcaaacacg tatgaatctt gagctggcgg ttactgaagc catgcttgat tttgccgagg aaatatcgcc ggagcatgct tgcttagtgc ctgaaaagcg ggaagaacta actacagaag gtggattgga tattttgact caccgaaatg tggtggaaaa ggcagtacgt cgcttacaat taatgggaag tgaggtttct ttatttattg acccggataa agagcaaata agagctgcgg ttgatgttgg ggcgccagtt atcgaattgc acactgggtg ttatgctgat gccacctcag aggaaaagca gcattatgaa ttgcaacgaa ttaaagaggc agctgaattt gctgccagtt taaacttggt agtgaatgcc ggtcatgggt tgcactatca taatgttaaa ccgattgcag cgattaggga gttaaatgaa cttaacatag gacatgccat tattgccaga gcattattct gtggattaaa agaggcagta agacacatgc gccaattaat gcaggaagnn ngactttatg tcaacgacta agtgagtaag at
Expressed Protein Sequence
MAHHHHHHMN KELLLGVNID HIATIRQARG TRYPDPVQAA MDAEEAGADG ITLHMREDLR HIQARDVRLI KQVLQTRMNL ELAVTEAMLD FAEEISPEHA CLVPEKREEL TTEGGLDILT HRNVVEKAVR RLQLMGSEVS LFIDPDKEQI RAAVDVGAPV IELHTGCYAD ATSEEKQHYE LQRIKEAAEF AASLNLVVNA GHGLHYHNVK PIAAIRELNE LNIGHAIIAR ALFCGLKEAV RHMRQLMQEA RLYVND
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA AAGAGTTATT GTTAGGTGTT AATATTGATC ATATAGCAAC CATAAGACAA GCACGAGGAA CTCGTTATCC TGATCCTGTC CAGGCAGCAA TGGATGCTGA AGAAGCAGGG GCAGATGGAA TTACTTTGCA TATGAGAGAG GACTTACGAC ATATTCAGGC GCGTGATGTC AGATTGATTA AACAGGTTCT GCAAACACGT ATGAATCTTG AGCTGGCGGT TACTGAAGCC ATGCTTGATT TTGCCGAGGA AATATCGCCG GAGCATGCTT GCTTAGTGCC TGAAAAGCGG GAAGAACTAA CTACAGAAGG TGGATTGGAT ATTTTGACTC ACCGAAATGT GGTGGAAAAG GCAGTACGTC GCTTACAATT AATGGGAAGT GAGGTTTCTT TATTTATTGA CCCGGATAAA GAGCAAATAA GAGCTGCGGT TGATGTTGGG GCGCCAGTTA TCGAATTGCA CACTGGGTGT TATGCTGATG CCACCTCAGA GGAAAAGCAG CATTATGAAT TGCAACGAAT TAAAGAGGCA GCTGAATTTG CTGCCAGTTT AAACTTGGTA GTGAATGCCG GTCATGGGTT GCACTATCAT AATGTTAAAC CGATTGCAGC GATTAGGGAG TTAAATGAAC TTAACATAGG ACATGCCATT ATTGCCAGAG CATTATTCTG TGGATTAAAA GAGGCAGTAA GACACATGCG CCAATTAATG CAGGAAGCGA GACTTTATGT CAACGACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT