LepnA.00177.a

Chaperone protein DnaK

CENTER ID: LepnA.00177.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00177.a.B1.GE41364 Full length(LepnA.00177.a) 1 644
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00177.a.B1.PS38434 Full length(LepnA.00177.a) 1 644

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.2121
UniProt: Q5ZTY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKIIGIDLG TTNSCVAVME GDKPKVIENS EGHRTTPSIV AFTDDNEILV GQSAKRQSVT NPEKTLFAIK RLIGRRFDDP IVQKDIKMVP YKIMKADNGD AWVRVKDQDK APPQISAEVL RKMKKTAEDY LGEEVKEAVI TVPAYFNDSQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG MDKKRGDSVI AVYDLGGGTF DISIIEIAEV DGEHQFEVLA TNGDTFLGGE DFDLALIEYL ASEFKKDTGI DLHNDPLALQ RLKEAAEKAK IELSSAQQTD VNLPYITADA SGPKHLNIKL TRAKLESLVE KLVERTIEPC KTALKDAGLT VSQINEVILV GGQTRMPLVQ KTVEEFFGKE PRKDVNPDEA VAVGAAIQAA VLSGEVKDIL LLDVTPLSLG IETMGGVMTK LIEKNTTIPT KATQVFSTAD DNQTAVTVHV LQGEREQASA NKSLGRFDLR DIPPAPRGVP QIEVTFDIDA NGILNVSAKD KATGKAQSIV IKASSGLSEE EVAAMVKDAQ SHAEEDKKFK EMAELRNQAD SLIHSCEKSM KDLADELSED EKRGIETAIS ELKEAVQGTD KARIEDKLKV LTDASAKMAE RIYAKKSSEG QAAQGQTQSQ ESTKPAEEGV VDAEFEEVKE EDKK
NT Sequence
ATGGCTAAAA TAATAGGTAT AGACCTGGGT ACTACGAACT CTTGTGTTGC TGTCATGGAA GGTGACAAAC CGAAGGTAAT TGAAAATAGT GAAGGTCACA GAACAACTCC TTCTATCGTA GCATTTACTG ATGATAATGA AATATTGGTA GGCCAATCAG CCAAGCGTCA ATCCGTGACT AACCCAGAGA AAACGCTGTT TGCGATTAAA CGATTAATTG GGCGTCGATT TGATGATCCT ATCGTGCAAA AAGATATTAA GATGGTTCCC TACAAAATTA TGAAAGCGGA CAATGGGGAT GCTTGGGTTA GAGTCAAAGA TCAAGATAAG GCACCACCAC AAATTTCTGC TGAAGTGTTA AGAAAAATGA AAAAAACAGC AGAAGATTAT TTGGGTGAAG AAGTAAAAGA AGCAGTGATT ACTGTACCTG CCTATTTCAA CGATTCGCAA CGCCAGGCAA CCAAAGATGC TGGTCGTATT GCTGGCCTGG AAGTAAAACG AATCATTAAT GAGCCTACCG CTGCTGCACT GGCTTATGGT ATGGATAAAA AACGCGGTGA CTCTGTTATT GCGGTATATG ATTTAGGGGG AGGAACTTTT GATATTTCAA TTATTGAAAT TGCCGAAGTG GATGGTGAAC ATCAATTTGA AGTACTTGCA ACCAATGGTG ATACCTTCCT CGGTGGAGAA GATTTTGATT TGGCTTTAAT TGAGTATTTG GCTTCTGAGT TCAAGAAAGA CACTGGTATT GATTTGCATA ATGATCCTCT TGCTCTGCAA CGCTTGAAAG AGGCTGCCGA GAAAGCAAAA ATTGAATTGT CATCTGCACA ACAAACTGAT GTCAATTTGC CTTACATTAC AGCAGATGCT TCTGGGCCAA AACATTTAAA TATCAAATTG ACTCGCGCAA AACTGGAATC TTTAGTGGAG AAACTGGTTG AGAGAACTAT TGAGCCATGT AAAACAGCTT TAAAAGATGC AGGGCTTACT GTCTCACAAA TCAACGAAGT TATTCTGGTC GGTGGGCAAA CTCGTATGCC TCTGGTACAA AAAACTGTGG AAGAATTCTT TGGCAAAGAA CCTCGTAAGG ATGTAAACCC TGATGAGGCT GTGGCAGTAG GTGCTGCAAT TCAGGCAGCA GTATTGTCCG GTGAAGTGAA AGACATTCTT TTACTCGATG TCACTCCTTT GTCTCTCGGT ATAGAAACCA TGGGTGGTGT AATGACCAAA CTCATTGAAA AAAACACAAC TATTCCTACA AAGGCTACAC AAGTGTTTTC AACAGCCGAT GATAATCAGA CAGCAGTGAC TGTGCATGTA TTGCAGGGTG AAAGAGAACA GGCTTCAGCA AACAAGTCAC TGGGACGGTT TGATTTGCGT GACATCCCTC CTGCTCCTCG TGGTGTTCCT CAAATTGAAG TAACTTTTGA TATTGATGCC AATGGTATCT TGAATGTTTC TGCCAAAGAC AAGGCAACTG GCAAGGCACA ATCCATTGTC ATCAAAGCTT CCAGTGGTTT AAGTGAAGAA GAGGTTGCTG CTATGGTGAA AGATGCCCAA TCGCATGCTG AAGAAGATAA GAAATTCAAG GAAATGGCAG AACTGCGCAA TCAGGCAGAT AGTTTGATTC ATAGCTGTGA AAAATCTATG AAAGATTTGG CTGATGAGTT GTCTGAGGAT GAGAAAAGAG GCATCGAAAC AGCAATTTCT GAGCTGAAAG AGGCGGTGCA AGGAACTGAC AAGGCTCGTA TTGAAGACAA GCTGAAAGTA TTGACAGACG CTTCCGCAAA AATGGCTGAA CGTATTTATG CTAAAAAATC GTCTGAAGGT CAGGCAGCTC AGGGGCAAAC ACAATCTCAG GAATCTACAA AACCTGCGGA AGAGGGAGTT GTTGATGCTG AGTTTGAAGA AGTTAAAGAA GAGGATAAAA AG
Details for LepnA.00177.a.B1.GE41364
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 71kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA KIIGIDLGTT NSCVAVMEGD KPKVIENSEG HRTTPSIVAF TDDNEILVGQ SAKRQSVTNP EKTLFAIKRL IGRRFDDPIV QKDIKMVPYK IMKADNGDAW VRVKDQDKAP PQISAEVLRK MKKTAEDYLG EEVKEAVITV PAYFNDSQRQ ATKDAGRIAG LEVKRIINEP TAAALAYGMD KKRGDSVIAV YDLGGGTFDI SIIEIAEVDG EHQFEVLATN GDTFLGGEDF DLALIEYLAS EFKKDTGIDL HNDPLALQRL KEAAEKAKIE LSSAQQTDVN LPYITADASG PKHLNIKLTR AKLESLVEKL VERTIEPCKT ALKDAGLTVS QINEVILVGG QTRMPLVQKT VEEFFGKEPR KDVNPDEAVA VGAAIQAAVL SGEVKDILLL DVTPLSLGIE TMGGVMTKLI EKNTTIPTKA TQVFSTADDN QTAVTVHVLQ GEREQASANK SLGRFDLRDI PPAPRGVPQI EVTFDIDANG ILNVSAKDKA TGKAQSIVIK ASSGLSEEEV AAMVKDAQSH AEEDKKFKEM AELRNQADSL IHSCEKSMKD LADELSEDEK RGIETAISEL KEAVQGTDKA RIEDKLKVLT DASAKMAERI YAKKSSEGQA AQGQTQSQES TKPAEEGVVD AEFEEVKEED KK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aaataatagg tatagacctg ggtactacga actcttgtgt tgctgtcatg gaaggtgaca aaccgaaggt aattgaaaat agtgaaggtc acagaacaac tccttctatc gtagcattta ctgatgataa tgaaatattg gtaggccaat cagccaagcg tcaatccgtg actaacccag agaaaacgct gtttgcgatt aaacgattaa ttgggcgtcg atttgatgat cctatcgtgc aaaaagatat taagatggtt ccctacaaaa ttatgaaagc ggacaatggg gatgcttggg ttagagtcaa agatcaagat aaggcaccac cacaaatttc tgctgaagtg ttaagaaaaa tgaaaaaaac agcagaagat tatttgggtg aagaagtaaa agaagcagtg attactgtac ctgcctattt caacgattcg caacgccagg caaccaaaga tgctggtcgt attgctggcc tggaagtaaa acgaatcatt aatgagccta ccgctgctgc actggcttat ggtatggata aaaaacgcgg tgactctgtt attgcggtat atgatttagg gggaggaact tttgatattt caattattga aattgccgaa gtggatggtg aacatcaatt tgaagtactt gcaaccaatg gtgatacctt cctcggtgga gaagattttg atttggcttt aattgagtat ttggcttctg agttcaagaa agacactggt attgatttgc ataatgatcc tcttgctctg caacgcttga aagaggctgc cgagaaagca aaaattgaat tgtcatctgc acaacaaact gatgtcaatt tgccttacat tacagcagat gcttctgggc caaaacattt aaatatcaaa ttgactcgcg caaaactgga atctttagtg gagaaactgg ttgagagaac tattgagcca tgtaaaacag ctttaaaaga tgcagggctt actgtctcac aaatcaacga agttattctg gtcggtgggc aaactcgtat gcctctggta caaaaaactg tggaagaatt ctttggcaaa gaacctcgta aggatgtaaa ccctgatgag gctgtggcag taggtgctgc aattcaggca gcagtattgt ccggtgaagt gaaagacatt cttttactcg atgtcactcc tttgtctctc ggtatagaaa ccatgggtgg tgtaatgacc aaactcattg aaaaaaacac aactattcct acaaaggcta cacaagtgtt ttcaacagcc gatgataatc agacagcagt gactgtgcat gtattgcagg gtgaaagaga acaggcttca gcaaacaagt cactgggacg gtttgatttg cgtgacatcc ctcctgctcc tcgtggtgtt cctcaaattg aagtaacttt tgatattgat gccaatggta tcttgaatgt ttctgccaaa gacaaggcaa ctggcaaggc acaatccatt gtcatcaaag cttccagtgg tttaagtgaa gaagaggttg ctgctatggt gaaagatgcc caatcgcatg ctgaagaaga taagaaattc aaggaaatgg cagaactgcg caatcaggca gatagtttga ttcatagctg tgaaaaatct atgaaagatt tggctgatga gttgtctgag gatgagaaaa gaggcatcga aacagcaatt tctgagctga aagaggcggt gcaaggaact gacaaggctc gtattgaaga caagctgaaa gtattgacag acgcttccgc aaaaatggct gaacgtattt atgctaaaaa atcgtctgaa ggtcaggcag ctcaggggca aacacaatct caggaatcta caaaacctgc ggaagaggga gttgttgatg ctgagtttga agaagttaaa gaagaggata aaaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LepnA.00177.a.B1.PS38434
PURIFICATION DATe: 3/29/2018
CONCENTRATION: 35.18mg/ml
OBSERVED MW: 80kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA KIIGIDLGTT NSCVAVMEGD KPKVIENSEG HRTTPSIVAF TDDNEILVGQ SAKRQSVTNP EKTLFAIKRL IGRRFDDPIV QKDIKMVPYK IMKADNGDAW VRVKDQDKAP PQISAEVLRK MKKTAEDYLG EEVKEAVITV PAYFNDSQRQ ATKDAGRIAG LEVKRIINEP TAAALAYGMD KKRGDSVIAV YDLGGGTFDI SIIEIAEVDG EHQFEVLATN GDTFLGGEDF DLALIEYLAS EFKKDTGIDL HNDPLALQRL KEAAEKAKIE LSSAQQTDVN LPYITADASG PKHLNIKLTR AKLESLVEKL VERTIEPCKT ALKDAGLTVS QINEVILVGG QTRMPLVQKT VEEFFGKEPR KDVNPDEAVA VGAAIQAAVL SGEVKDILLL DVTPLSLGIE TMGGVMTKLI EKNTTIPTKA TQVFSTADDN QTAVTVHVLQ GEREQASANK SLGRFDLRDI PPAPRGVPQI EVTFDIDANG ILNVSAKDKA TGKAQSIVIK ASSGLSEEEV AAMVKDAQSH AEEDKKFKEM AELRNQADSL IHSCEKSMKD LADELSEDEK RGIETAISEL KEAVQGTDKA RIEDKLKVLT DASAKMAERI YAKKSSEGQA AQGQTQSQES TKPAEEGVVD AEFEEVKEED KK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA AAATAATAGG TATAGACCTG GGTACTACGA ACTCTTGTGT TGCTGTCATG GAAGGTGACA AACCGAAGGT AATTGAAAAT AGTGAAGGTC ACAGAACAAC TCCTTCTATC GTAGCATTTA CTGATGATAA TGAAATATTG GTAGGCCAAT CAGCCAAGCG TCAATCCGTG ACTAACCCAG AGAAAACGCT GTTTGCGATT AAACGATTAA TTGGGCGTCG ATTTGATGAT CCTATCGTGC AAAAAGATAT TAAGATGGTT CCCTACAAAA TTATGAAAGC GGACAATGGG GATGCTTGGG TTAGAGTCAA AGATCAAGAT AAGGCACCAC CACAAATTTC TGCTGAAGTG TTAAGAAAAA TGAAAAAAAC AGCAGAAGAT TATTTGGGTG AAGAAGTAAA AGAAGCAGTG ATTACTGTAC CTGCCTATTT CAACGATTCG CAACGCCAGG CAACCAAAGA TGCTGGTCGT ATTGCTGGCC TGGAAGTAAA ACGAATCATT AATGAGCCTA CCGCTGCTGC ACTGGCTTAT GGTATGGATA AAAAACGCGG TGACTCTGTT ATTGCGGTAT ATGATTTAGG GGGAGGAACT TTTGATATTT CAATTATTGA AATTGCCGAA GTGGATGGTG AACATCAATT TGAAGTACTT GCAACCAATG GTGATACCTT CCTCGGTGGA GAAGATTTTG ATTTGGCTTT AATTGAGTAT TTGGCTTCTG AGTTCAAGAA AGACACTGGT ATTGATTTGC ATAATGATCC TCTTGCTCTG CAACGCTTGA AAGAGGCTGC CGAGAAAGCA AAAATTGAAT TGTCATCTGC ACAACAAACT GATGTCAATT TGCCTTACAT TACAGCAGAT GCTTCTGGGC CAAAACATTT AAATATCAAA TTGACTCGCG CAAAACTGGA ATCTTTAGTG GAGAAACTGG TTGAGAGAAC TATTGAGCCA TGTAAAACAG CTTTAAAAGA TGCAGGGCTT ACTGTCTCAC AAATCAACGA AGTTATTCTG GTCGGTGGGC AAACTCGTAT GCCTCTGGTA CAAAAAACTG TGGAAGAATT CTTTGGCAAA GAACCTCGTA AGGATGTAAA CCCTGATGAG GCTGTGGCAG TAGGTGCTGC AATTCAGGCA GCAGTATTGT CCGGTGAAGT GAAAGACATT CTTTTACTCG ATGTCACTCC TTTGTCTCTC GGTATAGAAA CCATGGGTGG TGTAATGACC AAACTCATTG AAAAAAACAC AACTATTCCT ACAAAGGCTA CACAAGTGTT TTCAACAGCC GATGATAATC AGACAGCAGT GACTGTGCAT GTATTGCAGG GTGAAAGAGA ACAGGCTTCA GCAAACAAGT CACTGGGACG GTTTGATTTG CGTGACATCC CTCCTGCTCC TCGTGGTGTT CCTCAAATTG AAGTAACTTT TGATATTGAT GCCAATGGTA TCTTGAATGT TTCTGCCAAA GACAAGGCAA CTGGCAAGGC ACAATCCATT GTCATCAAAG CTTCCAGTGG TTTAAGTGAA GAAGAGGTTG CTGCTATGGT GAAAGATGCC CAATCGCATG CTGAAGAAGA TAAGAAATTC AAGGAAATGG CAGAACTGCG CAATCAGGCA GATAGTTTGA TTCATAGCTG TGAAAAATCT ATGAAAGATT TGGCTGATGA GTTGTCTGAG GATGAGAAAA GAGGCATCGA AACAGCAATT TCTGAGCTGA AAGAGGCGGT GCAAGGAACT GACAAGGCTC GTATTGAAGA CAAGCTGAAA GTATTGACAG ACGCTTCCGC AAAAATGGCT GAACGTATTT ATGCTAAAAA ATCGTCTGAA GGTCAGGCAG CTCAGGGGCA AACACAATCT CAGGAATCTA CAAAACCTGC GGAAGAGGGA GTTGTTGATG CTGAGTTTGA AGAAGTTAAA GAAGAGGATA AAAAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT