LepnA.00156.a

Threonine--tRNA ligase

CENTER ID: LepnA.00156.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00156.a.B1.GE41359 Full length(LepnA.00156.a) 1 637
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00156.a.B1.PW38307 Full length(LepnA.00156.a) 1 637

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.2900
UniProt: Q5ZS05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPNVKLPDGN VKHFEAPLTI YDVAHHISPG LAKAAIAGRV DGVLVDTSYL IKEDCSLIIV TEKHEDSLEI IRHSTAHLLA QAVKALFPSA QVTIGPVIED GFYYDFAFER SFTPDDLSLI EAKMHELAKA NLSITRRELP RNEAIQYFKD LGEEYKAKII ADIPENEALS LYRQGDFEDL CRGPHVPSTG FLKAFKLTKV AGAYWRGDSN NEMLQRIYGT AWADKKSLEE YLFRLEEAEK RDHRKLGKAL DLFHFQDIAP GMVFWHPKGW TIYQELEHYM RNRLVDFGYQ EIRTPQLVDR SLWEKSGHWA NFRDEMFVTE TENRHYAVKP MSCPCHVQIY NHGLKSYRDL PLRLSEFGNC HRCEPSGALH GLMRVRNMVQ DDAHIFCTED QIQSEVAMML ELVQSVYKDF GFTEIKYRLA LRPEKRVGSD DVWDKAETAL KLAMQGRNIE WVDAPGEGAF YGPKIECSLS DCLGRIWQCG TIQVDFSMPA RLEASYVAED GSKQTPVMLH RAILGSFERF MGILIEHYAG KLPLWLSPVQ AIVLTISEKQ NEYAEKVRKT LQKRGIRANF DLRNEKIGFK IREHTLQKIP YLLVVGDKEV ENCQVAVRTR DGIDLGVMTI DTICDTLTQE IIRKGSI
NT Sequence
ATGCCAAATG TTAAATTACC AGATGGAAAT GTAAAACATT TTGAAGCGCC ACTCACTATT TATGATGTGG CACATCACAT TAGCCCTGGA TTAGCTAAAG CAGCGATAGC AGGCCGTGTC GATGGCGTCT TGGTGGATAC ATCCTATCTT ATAAAAGAAG ACTGCTCTTT GATTATCGTA ACCGAGAAAC ATGAAGACAG TCTTGAAATA ATCAGGCATT CTACCGCTCA TTTATTAGCT CAGGCTGTAA AAGCCTTATT TCCATCCGCT CAAGTGACTA TAGGTCCTGT AATAGAAGAC GGGTTTTATT ATGATTTTGC TTTTGAGCGC TCGTTTACTC CCGATGATTT GAGTTTAATC GAAGCGAAGA TGCATGAGCT GGCAAAGGCT AATCTTTCGA TCACTCGTCG AGAATTACCT AGAAATGAAG CAATACAATA TTTTAAGGAT TTAGGCGAAG AATATAAGGC AAAAATTATT GCTGATATAC CCGAAAATGA AGCGCTCTCT CTATATAGGC AAGGTGATTT TGAAGATTTG TGTCGAGGTC CACATGTCCC CTCCACTGGG TTTTTAAAAG CATTTAAATT GACAAAAGTA GCTGGCGCTT ATTGGCGTGG TGACTCAAAT AACGAAATGT TGCAGCGAAT TTACGGCACT GCCTGGGCTG ATAAAAAATC CCTGGAAGAA TATTTGTTTC GTTTGGAAGA AGCAGAAAAA CGTGATCATC GTAAATTAGG CAAGGCACTG GATTTGTTTC ATTTCCAGGA TATTGCTCCG GGAATGGTTT TTTGGCACCC CAAAGGATGG ACTATTTATC AAGAGCTAGA GCATTACATG CGAAATCGCC TGGTCGATTT TGGCTATCAG GAAATTAGAA CACCGCAATT AGTAGACAGA TCGTTATGGG AGAAATCAGG TCATTGGGCG AACTTTAGAG ATGAAATGTT TGTTACCGAA ACAGAGAATC GACATTATGC AGTAAAACCT ATGAGCTGTC CCTGCCACGT TCAAATTTAT AATCATGGTT TGAAAAGTTA CCGGGATCTT CCTCTCAGAC TATCTGAGTT TGGAAATTGT CATCGCTGTG AGCCATCAGG CGCGCTGCAT GGGTTGATGC GGGTACGCAA TATGGTGCAG GATGATGCTC ATATTTTTTG CACTGAGGAT CAGATTCAAT CTGAAGTGGC TATGATGCTG GAGTTAGTGC AATCCGTATA TAAGGACTTT GGGTTTACTG AAATAAAATA CCGATTGGCT TTGCGCCCTG AAAAACGAGT CGGTTCAGAT GACGTTTGGG ATAAGGCGGA AACCGCCCTT AAACTGGCTA TGCAGGGAAG AAATATTGAG TGGGTGGATG CGCCTGGAGA AGGAGCATTT TATGGACCTA AAATTGAATG CTCTCTCTCT GATTGTTTAG GTAGAATTTG GCAGTGCGGA ACGATTCAAG TGGATTTCTC TATGCCTGCA CGCCTTGAAG CAAGCTATGT TGCTGAAGAT GGAAGCAAGC AAACACCGGT GATGTTGCAT AGAGCCATTT TGGGTTCTTT CGAACGATTT ATGGGAATAT TAATCGAACA TTATGCAGGT AAATTGCCAT TATGGTTATC CCCAGTGCAA GCGATTGTGC TTACTATTAG TGAGAAGCAG AATGAGTATG CCGAAAAAGT GAGGAAAACT TTGCAAAAAA GAGGGATTCG CGCCAATTTT GACTTGAGAA ATGAAAAGAT TGGATTTAAA ATTCGCGAGC ATACTTTACA AAAAATTCCT TATTTATTGG TTGTTGGCGA TAAAGAGGTT GAAAATTGTC AGGTAGCTGT TCGCACTCGA GATGGCATTG ACCTGGGTGT GATGACAATA GATACAATTT GTGATACCTT AACGCAAGAA ATTATCCGTA AAGGATCAAT A
Details for LepnA.00156.a.B1.GE41359
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 74kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP NVKLPDGNVK HFEAPLTIYD VAHHISPGLA KAAIAGRVDG VLVDTSYLIK EDCSLIIVTE KHEDSLEIIR HSTAHLLAQA VKALFPSAQV TIGPVIEDGF YYDFAFERSF TPDDLSLIEA KMHELAKANL SITRRELPRN EAIQYFKDLG EEYKAKIIAD IPENEALSLY RQGDFEDLCR GPHVPSTGFL KAFKLTKVAG AYWRGDSNNE MLQRIYGTAW ADKKSLEEYL FRLEEAEKRD HRKLGKALDL FHFQDIAPGM VFWHPKGWTI YQELEHYMRN RLVDFGYQEI RTPQLVDRSL WEKSGHWANF RDEMFVTETE NRHYAVKPMS CPCHVQIYNH GLKSYRDLPL RLSEFGNCHR CEPSGALHGL MRVRNMVQDD AHIFCTEDQI QSEVAMMLEL VQSVYKDFGF TEIKYRLALR PEKRVGSDDV WDKAETALKL AMQGRNIEWV DAPGEGAFYG PKIECSLSDC LGRIWQCGTI QVDFSMPARL EASYVAEDGS KQTPVMLHRA ILGSFERFMG ILIEHYAGKL PLWLSPVQAI VLTISEKQNE YAEKVRKTLQ KRGIRANFDL RNEKIGFKIR EHTLQKIPYL LVVGDKEVEN CQVAVRTRDG IDLGVMTIDT ICDTLTQEII RKGSI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa atgttaaatt accagatgga aatgtaaaac attttgaagc gccactcact atttatgatg tggcacatca cattagccct ggattagcta aagcagcgat agcaggccgt gtcgatggcg tcttggtgga tacatcctat cttataaaag aagactgctc tttgattatc gtaaccgaga aacatgaaga cagtcttgaa ataatcaggc attctaccgc tcatttatta gctcaggctg taaaagcctt atttccatcc gctcaagtga ctataggtcc tgtaatagaa gacgggtttt attatgattt tgcttttgag cgctcgttta ctcccgatga tttgagttta atcgaagcga agatgcatga gctggcaaag gctaatcttt cgatcactcg tcgagaatta cctagaaatg aagcaataca atattttaag gatttaggcg aagaatataa ggcaaaaatt attgctgata tacccgaaaa tgaagcgctc tctctatata ggcaaggtga ttttgaagat ttgtgtcgag gtccacatgt cccctccact gggtttttaa aagcatttaa attgacaaaa gtagctggcg cttattggcg tggtgactca aataacgaaa tgttgcagcg aatttacggc actgcctggg ctgataaaaa atccctggaa gaatatttgt ttcgtttgga agaagcagaa aaacgtgatc atcgtaaatt aggcaaggca ctggatttgt ttcatttcca ggatattgct ccgggaatgg ttttttggca ccccaaagga tggactattt atcaagagct agagcattac atgcgaaatc gcctggtcga ttttggctat caggaaatta gaacaccgca attagtagac agatcgttat gggagaaatc aggtcattgg gcgaacttta gagatgaaat gtttgttacc gaaacagaga atcgacatta tgcagtaaaa cctatgagct gtccctgcca cgttcaaatt tataatcatg gtttgaaaag ttaccgggat cttcctctca gactatctga gtttggaaat tgtcatcgct gtgagccatc aggcgcgctg catgggttga tgcgggtacg caatatggtg caggatgatg ctcatatttt ttgcactgag gatcagattc aatctgaagt ggctatgatg ctggagttag tgcaatccgt atataaggac tttgggttta ctgaaataaa ataccgattg gctttgcgcc ctgaaaaacg agtcggttca gatgacgttt gggataaggc ggaaaccgcc cttaaactgg ctatgcaggg aagaaatatt gagtgggtgg atgcgcctgg agaaggagca ttttatggac ctaaaattga atgctctctc tctgattgtt taggtagaat ttggcagtgc ggaacgattc aagtggattt ctctatgcct gcacgccttg aagcaagcta tgttgctgaa gatggaagca agcaaacacc ggtgatgttg catagagcca ttttgggttc tttcgaacga tttatgggaa tattaatcga acattatgca ggtaaattgc cattatggtt atccccagtg caagcgattg tgcttactat tagtgagaag cagaatgagt atgccgaaaa agtgaggaaa actttgcaaa aaagagggat tcgcgccaat tttgacttga gaaatgaaaa gattggattt aaaattcgcg agcatacttt acaaaaaatt ccttatttat tggttgttgg cgataaagag gttgaaaatt gtcaggtagc tgttcgcact cgagatggca ttgacctggg tgtgatgaca atagatacaa tttgtgatac cttaacgcaa gaaattatcc gtaaaggatc aatatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LepnA.00156.a.B1.PW38307
PURIFICATION DATe: 7/5/2017
CONCENTRATION: 60.22mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
IMAC followed by SEC separation and concentration
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMP NVKLPDGNVK HFEAPLTIYD VAHHISPGLA KAAIAGRVDG VLVDTSYLIK EDCSLIIVTE KHEDSLEIIR HSTAHLLAQA VKALFPSAQV TIGPVIEDGF YYDFAFERSF TPDDLSLIEA KMHELAKANL SITRRELPRN EAIQYFKDLG EEYKAKIIAD IPENEALSLY RQGDFEDLCR GPHVPSTGFL KAFKLTKVAG AYWRGDSNNE MLQRIYGTAW ADKKSLEEYL FRLEEAEKRD HRKLGKALDL FHFQDIAPGM VFWHPKGWTI YQELEHYMRN RLVDFGYQEI RTPQLVDRSL WEKSGHWANF RDEMFVTETE NRHYAVKPMS CPCHVQIYNH GLKSYRDLPL RLSEFGNCHR CEPSGALHGL MRVRNMVQDD AHIFCTEDQI QSEVAMMLEL VQSVYKDFGF TEIKYRLALR PEKRVGSDDV WDKAETALKL AMQGRNIEWV DAPGEGAFYG PKIECSLSDC LGRIWQCGTI QVDFSMPARL EASYVAEDGS KQTPVMLHRA ILGSFERFMG ILIEHYAGKL PLWLSPVQAI VLTISEKQNE YAEKVRKTLQ KRGIRANFDL RNEKIGFKIR EHTLQKIPYL LVVGDKEVEN CQVAVRTRDG IDLGVMTIDT ICDTLTQEII RKGSI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAA ATGTTAAATT ACCAGATGGA AATGTAAAAC ATTTTGAAGC GCCACTCACT ATTTATGATG TGGCACATCA CATTAGCCCT GGATTAGCTA AAGCAGCGAT AGCAGGCCGT GTCGATGGCG TCTTGGTGGA TACATCCTAT CTTATAAAAG AAGACTGCTC TTTGATTATC GTAACCGAGA AACATGAAGA CAGTCTTGAA ATAATCAGGC ATTCTACCGC TCATTTATTA GCTCAGGCTG TAAAAGCCTT ATTTCCATCC GCTCAAGTGA CTATAGGTCC TGTAATAGAA GACGGGTTTT ATTATGATTT TGCTTTTGAG CGCTCGTTTA CTCCCGATGA TTTGAGTTTA ATCGAAGCGA AGATGCATGA GCTGGCAAAG GCTAATCTTT CGATCACTCG TCGAGAATTA CCTAGAAATG AAGCAATACA ATATTTTAAG GATTTAGGCG AAGAATATAA GGCAAAAATT ATTGCTGATA TACCCGAAAA TGAAGCGCTC TCTCTATATA GGCAAGGTGA TTTTGAAGAT TTGTGTCGAG GTCCACATGT CCCCTCCACT GGGTTTTTAA AAGCATTTAA ATTGACAAAA GTAGCTGGCG CTTATTGGCG TGGTGACTCA AATAACGAAA TGTTGCAGCG AATTTACGGC ACTGCCTGGG CTGATAAAAA ATCCCTGGAA GAATATTTGT TTCGTTTGGA AGAAGCAGAA AAACGTGATC ATCGTAAATT AGGCAAGGCA CTGGATTTGT TTCATTTCCA GGATATTGCT CCGGGAATGG TTTTTTGGCA CCCCAAAGGA TGGACTATTT ATCAAGAGCT AGAGCATTAC ATGCGAAATC GCCTGGTCGA TTTTGGCTAT CAGGAAATTA GAACACCGCA ATTAGTAGAC AGATCGTTAT GGGAGAAATC AGGTCATTGG GCGAACTTTA GAGATGAAAT GTTTGTTACC GAAACAGAGA ATCGACATTA TGCAGTAAAA CCTATGAGCT GTCCCTGCCA CGTTCAAATT TATAATCATG GTTTGAAAAG TTACCGGGAT CTTCCTCTCA GACTATCTGA GTTTGGAAAT TGTCATCGCT GTGAGCCATC AGGCGCGCTG CATGGGTTGA TGCGGGTACG CAATATGGTG CAGGATGATG CTCATATTTT TTGCACTGAG GATCAGATTC AATCTGAAGT GGCTATGATG CTGGAGTTAG TGCAATCCGT ATATAAGGAC TTTGGGTTTA CTGAAATAAA ATACCGATTG GCTTTGCGCC CTGAAAAACG AGTCGGTTCA GATGACGTTT GGGATAAGGC GGAAACCGCC CTTAAACTGG CTATGCAGGG AAGAAATATT GAGTGGGTGG ATGCGCCTGG AGAAGGAGCA TTTTATGGAC CTAAAATTGA ATGCTCTCTC TCTGATTGTT TAGGTAGAAT TTGGCAGTGC GGAACGATTC AAGTGGATTT CTCTATGCCT GCACGCCTTG AAGCAAGCTA TGTTGCTGAA GATGGAAGCA AGCAAACACC GGTGATGTTG CATAGAGCCA TTTTGGGTTC TTTCGAACGA TTTATGGGAA TATTAATCGA ACATTATGCA GGTAAATTGC CATTATGGTT ATCCCCAGTG CAAGCGATTG TGCTTACTAT TAGTGAGAAG CAGAATGAGT ATGCCGAAAA AGTGAGGAAA ACTTTGCAAA AAAGAGGGAT TCGCGCCAAT TTTGACTTGA GAAATGAAAA GATTGGATTT AAAATTCGCG AGCATACTTT ACAAAAAATT CCTTATTTAT TGGTTGTTGG CGATAAAGAG GTTGAAAATT GTCAGGTAGC TGTTCGCACT CGAGATGGCA TTGACCTGGG TGTGATGACA ATAGATACAA TTTGTGATAC CTTAACGCAA GAAATTATCC GTAAAGGATC AATATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT