LepnA.00150.a

Bifunctional protein GlmU

CENTER ID:	LepnA.00150.a
ORGANISM:	Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LepnA.00150.a.B1.GE41358	Full length(LepnA.00150.a)		1	461

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|272624.6.peg.3062
UniProt:	Q5ZRK6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLQIIILAA GQGKRMYSDT PKVLHHLAGK PLLTHVVETA QQLNPNAIHV IYGHGGEQIK SSLPNLPVHW VHQAEQLGTG HAVLQAMPHI PDDAYVLVLS ADVPLIQVET LQSLIECSQR QNPDHSVLAL LVAELENPSG LGRIIRNNQG EIYSIVEEKD ANEQVKNIKE IYSGVCCTLA NNLKKWLPQL SNSNAQGEYY LTEIISLAVQ NKTPITSLTA KNSFEVQGIN NRQQLQQLER IWQQRAANQL MEKGATLADA NRFDLRGELY CGKDVYIDIN CIFTGKVVLG NGCKIGPNCS LTNVTLGDGC EVYANSVLEG CHIANDCHIG PFARLRSGTQ LASHCKIGNF VETKKAIFDE GTKASHLSYL GDVLLGKNVN VGAGTITCNY DGVNKHQTII EDGVFIGSDT QLIAPVTVGA NATIGAGSTI RRNVPPDELT LTESRQKTIY GWKRPVKRER D

NT Sequence

ATGAATTTAC AAATTATTAT TTTAGCTGCA GGGCAAGGTA AAAGAATGTA TTCGGACACG CCCAAAGTTC TTCACCACCT AGCTGGCAAA CCGCTGTTAA CCCACGTCGT GGAAACCGCT CAGCAACTAA ATCCCAATGC AATTCATGTC ATTTATGGTC ATGGTGGTGA ACAAATTAAA AGTTCACTCC CCAACTTACC TGTTCATTGG GTGCATCAGG CAGAGCAACT AGGGACTGGC CATGCTGTTT TGCAAGCCAT GCCCCATATC CCTGACGATG CATACGTTCT GGTTTTATCT GCCGATGTTC CCCTGATACA AGTGGAAACT CTTCAATCAT TAATTGAATG CAGCCAACGT CAAAATCCAG ATCATTCTGT TTTAGCACTC CTGGTTGCAG AACTGGAAAA TCCAAGCGGT CTTGGGCGTA TTATCAGAAA CAACCAAGGT GAAATTTATA GCATTGTAGA AGAAAAGGAT GCAAATGAGC AAGTAAAGAA TATAAAAGAA ATTTATTCCG GGGTGTGTTG TACTTTGGCA AATAATCTGA AGAAATGGTT GCCTCAACTA AGTAATAGTA ACGCACAAGG CGAATATTAT TTAACTGAAA TTATCTCATT GGCTGTACAA AATAAAACAC CCATTACGTC ATTAACCGCC AAGAACAGCT TTGAGGTACA GGGCATTAAT AATCGCCAAC AACTTCAGCA ACTCGAACGC ATCTGGCAGC AAAGAGCAGC CAATCAACTC ATGGAAAAAG GCGCGACCCT GGCTGATGCC AACCGCTTTG ATTTGAGAGG AGAATTATAT TGTGGCAAAG ACGTCTATAT CGACATTAAC TGCATTTTTA CAGGCAAGGT AGTTTTAGGT AATGGCTGTA AAATTGGCCC AAACTGCAGT CTGACTAATG TGACATTAGG CGATGGGTGC GAAGTTTACG CTAATAGCGT ACTGGAAGGC TGCCACATTG CAAACGACTG CCACATCGGT CCTTTTGCCC GCTTGCGCTC AGGGACGCAA TTAGCTTCCC ATTGTAAAAT CGGCAATTTT GTTGAAACTA AAAAAGCGAT TTTTGATGAA GGCACGAAAG CCAGTCATCT CAGTTACTTA GGGGACGTCC TGTTAGGAAA AAATGTAAAC GTTGGCGCTG GAACCATTAC TTGTAATTAC GATGGGGTTA ATAAGCATCA AACAATTATC GAAGATGGTG TTTTTATTGG GTCAGATACT CAATTAATTG CTCCAGTTAC CGTGGGCGCT AACGCAACAA TAGGAGCAGG GAGCACCATA CGCCGGAATG TGCCGCCCGA TGAGTTAACA TTAACCGAAT CACGACAAAA AACCATCTAC GGCTGGAAAA GGCCGGTAAA AAGAGAGAGG GAT

Details for LepnA.00150.a.B1.GE41358

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/10/2017
EXPECTED MW:	51kDa
OBSERVED MW:	15kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMN LQIIILAAGQ GKRMYSDTPK VLHHLAGKPL LTHVVETAQQ LNPNAIHVIY GHGGEQIKSS LPNLPVHWVH QAEQLGTGHA VLQAMPHIPD DAYVLVLSAD VPLIQVETLQ SLIECSQRQN PDHSVLALLV AELENPSGLG RIIRNNQGEI YSIVEEKDAN EQVKNIKEIY SGVCCTLANN LKKWLPQLSN SNAQGEYYLT EIISLAVQNK TPITSLTAKN SFEVQGINNR QQLQQLERIW QQRAANQLME KGATLADANR FDLRGELYCG KDVYIDINCI FTGKVVLGNG CKIGPNCSLT NVTLGDGCEV YANSVLEGCH IANDCHIGPF ARLRSGTQLA SHCKIGNFVE TKKAIFDEGT KASHLSYLGD VLLGKNVNVG AGTITCNYDG VNKHQTIIED GVFIGSDTQL IAPVTVGANA TIGAGSTIRR NVPPDELTLT ESRQKTIYGW

Validated NT Sequence

tttccagccg tagatggttt tttgtcgtga ttcggttaat gttaactcat cgggcggcac attccggcgt atggtgctcc ctgctcctat tgttgcgtta gcgcccacgg taactggagc aattaattga gtatctgacc caataaaaac accatcttcg ataattgttt gatgcttatt aaccccatcg taattacaag taatggttcc agcgccaacg tttacatttt ttcctaacag gacgtcccct aagtaactga gatgactggc tttcgtgcct tcatcaaaaa tcgctttttt agtttcaaca aaattgccga ttttacaatg ggaagctaat tgcgtccctg agcgcaagcg ggcaaaagga ccgatgtggc agtcgtttgc aatgtggcag ccttccagta cgctattagc gtaaacttcg cacccatcgc ctaatgtcac attagtcaga ctgcagtttg ggccaatttt acagccatta cctaaaacta ccttgcctgt aaaaatgcag ttaatgtcga tatagacgtc tttgccacaa tataattctc ctctcaaatc aaagcggttg gcatcagcca gggtcgcgcc tttttccatg agttgattgg ctgctctttg ctgccagatg cgttcgagtt gctgaagttg ttggcgatta ttaatgccct gtacctcaaa gctgttcttg gcggttaatg acgtaatggg tgttttattt tgtacagcca atgagataat ttcagttaaa taatattcgc cttgtgcgtt actattactt agttgaggca accatttctt cagattattt gccaaagtac aacacacccc ggaataaatt tcttttatat tctttacttg ctcatttgca tccttttctt ctacaatgct ataaatttca ccttggttgt ttctgataat acgcccaaga ccgcttggat tttccagttc tgcaaccagg agtgctaaaa cagaatgatc tggattttga cgttggctgc attcaattaa tgattgaaga gtttccactt gtatcagggg aacatcggca gataaaacca gaacgtatgc atcgtcaggg atatggggca tggcttgcaa aacagcatgg ccagtcccta gttgctctgc ctgatgcacc caatgaacag gtaagttggg gagtgaactt ttaatttgtt caccaccatg accataaatg acatgaattg cattgggatt tagttgctga gcggtttcca cgacgtgggt taacagcggt ttgccagcta ggtggtgaag aactttgggc gtgtccgaat acattctttt accttgccct gcagctaaaa taataatttg taaattcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt tacaaattat tattttagct gcagggcaag gtaaaagaat gtattcggac acgcccaaag ttcttcacca cctagctggc aaaccgctgt taacccacgt cgtggaaacc gctcagcaac taaatcccaa tgcaattcat gtcatttatg gtcatggtgg tgaacaaatt aaaagttcac tccccaactt acctgttcat tgggtgcatc aggcagagca actagggact ggccatgctg ttttgcaagc catgccccat atccctgacg atgcatacgt tctggtttta tctgccgatg ttcccctgat acaagtggaa actcttcaat cattaattga atgcagccaa cgtcaaaatc cagatcattc tgttttagca ctcctggttg cagaactgga aaatccaagc ggtcttgggc gtattatcag aaacaaccaa ggtgaaattt atagcattgt agaagaaaag gatgcaaatg agcaagtaaa gaatataaaa gaaatttatt ccggggtgtg ttgtactttg gcaaataatc tgaagaaatg gttgcctcaa ctaagtaata gtaacgcaca aggcgaatat tatttaactg aaattatctc attggctgta caaaataaaa cacccattac gtcattaacc gccaagaaca gctttgaggt acagggcatt aataatcgcc aacaacttca gcaactcgaa cgcatctggc agcaaagagc agccaatcaa ctcatggaaa aaggcgcgac cctggctgat gccaaccgct ttgatttgag aggagaatta tattgtggca aagacgtcta tatcgacatt aactgcattt ttacaggcaa ggtagtttta ggtaatggct gtaaaattgg cccaaactgc agtctgacta atgtgacatt aggcgatggg tgcgaagttt acgctaatag cgtactggaa ggctgccaca ttgcaaacga ctgccacatc ggtccttttg cccgcttgcg ctcagggacg caattagctt cccattgtaa aatcggcaat tttgttgaaa ctaaaaaagc gatttttgat gaaggcacga aagccagtca tctcagttac ttaggggacg tcctgttagg aaaaaatgta aacgttggcg ctggaaccat tacttgtaat tacgatgggg ttaataagca tcaaacaatt atcgaagatg gtgtttttat tgggtcagat actcaattaa ttgctccagt taccgtgggc gctaacgcaa caataggagc agggagcacc atacgccgga atgtgccgcc cgatgagtta acattaaccg aatcacgaca aaaaaccatc tacggctgga aaaggccggt aaaaagagag agggattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t