LepnA.00116.a
4-hydroxythreonine-4-phosphate dehydrogenase
| CENTER ID: | LepnA.00116.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00116.a.B1.GE41355 | Full length(LepnA.00116.a) | 1 | 324 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00116.a.B1.PS38420 | Full length(LepnA.00116.a) | 1 | 324 |
Structures
6XMY
DEPOSITED: 7/1/2020
DETERMINATION: XRay
CLONE: LepnA.00116.a.B1.GE41355
PROTEIN: LepnA.00116.a.B1.PS38420
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.319 |
| UniProt: | Q3V872 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPLLISSGE PAGIGPDLCL ALAETDLPVV ILGDLSLLEA RASELNLSIK FLEYSPHQSF KKKAGYLTVW PVPCAAPVIS GELNPQNAAY VMELLTLGAS LCSKGEFSAL VTAPVHKANI NAAGITFTGH TEFFADFFEV ETVVMMLACS QMKVALVTTH LPLRMVPDAI SSLLIIKVIQ QLHHSLKHDF GIQSPKINVA GLNPHAGESG YLGREEIEII TPALNTLKNQ GIDVLGPLPA DTMFITNHIN HCDAYVAMYH DQGLPVLKYA GFNEAVNITL GLPIIRTSVD HGTALELAGK NKANPGSMLA AVKMAKDMAL TRIK
NT Sequence
ATGAACCCAC TGCTTATTAG TAGTGGAGAG CCTGCTGGAA TTGGCCCGGA CTTATGCCTG GCTTTGGCAG AAACCGATTT GCCCGTAGTG ATATTGGGTG ATCTGTCTTT ATTAGAGGCA AGAGCCAGTG AGTTAAATCT CAGTATTAAA TTTTTAGAGT ACAGCCCTCA TCAAAGCTTC AAAAAAAAAG CAGGATATTT AACTGTATGG CCTGTTCCAT GCGCTGCACC GGTCATTAGT GGTGAATTAA ATCCGCAAAA TGCGGCTTAT GTTATGGAGC TTTTGACTTT AGGGGCTTCG CTATGCTCCA AAGGGGAGTT TTCTGCATTA GTGACAGCGC CTGTTCATAA GGCAAACATT AATGCAGCCG GTATTACGTT TACTGGCCAC ACGGAGTTTT TTGCTGATTT CTTTGAGGTG GAAACAGTGG TGATGATGTT GGCCTGTTCT CAAATGAAAG TGGCATTAGT CACAACGCAT CTTCCTCTGC GTATGGTGCC TGATGCCATT AGCTCTTTGC TTATCATAAA GGTAATTCAA CAATTACATC ATTCATTAAA ACATGATTTT GGAATTCAAA GTCCAAAAAT CAACGTGGCT GGTCTGAATC CGCATGCTGG AGAATCAGGA TATTTAGGTC GAGAAGAAAT TGAAATTATT ACTCCAGCAT TAAATACTCT AAAAAACCAG GGGATCGATG TGTTAGGGCC GTTACCAGCA GATACGATGT TTATAACAAA CCATATCAAT CACTGTGATG CTTATGTGGC AATGTACCAT GATCAAGGAC TGCCAGTCTT AAAGTATGCT GGATTTAATG AAGCAGTAAA TATAACTCTC GGATTGCCGA TAATTCGTAC TTCTGTAGAT CATGGAACAG CATTAGAATT AGCTGGTAAG AACAAAGCTA ACCCTGGGAG TATGCTTGCG GCGGTTAAGA TGGCGAAGGA TATGGCTTTA ACAAGGATAA AA
Details for LepnA.00116.a.B1.GE41355
| HARVESTED ON: | 4/3/2017 |
| SEQUENCED ON: | 4/10/2017 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN PLLISSGEPA GIGPDLCLAL AETDLPVVIL GDLSLLEARA SELNLSIKFL EYSPHQSFKK KAGYLTVWPV PCAAPVISGE LNPQNAAYVM ELLTLGASLC SKGEFSALVT APVHKANINA AGITFTGHTE FFADFFEVET VVMMLACSQM KVALVTTHLP LRMVPDAISS LLIIKVIQQL HHSLKHDFGI QSPKINVAGL NPHAGESGYL GREEIEIITP ALNTLKNQGI DVLGPLPADT MFITNHINHC DAYVAMYHDQ GLPVLKYAGF NEAVNITLGL PIIRTSVDHG TALELAGKNK ANPGSMLAAV KMAKD
Validated NT Sequence
tatccttcgc catcttaacc gccgcaagca tactcccagg gttagctttg ttcttaccag ctaattctaa tgctgttcca tgatctacag aagtacgaat tatcggcaat ccgagagtta tatttactgc ttcattaaat ccagcatact ttaagactgg cagtccttga tcatggtaca ttgccacata agcatcacag tgattgatat ggtttgttat aaacatcgta tctgctggta acggccctaa cacatcgatc ccctggtttt ttagagtatt taatgctgga gtaataattt caatttcttc tcgacctaaa tatcctgatt ctccagcatg cggattcaga ccagccacgt tgatttttgg actttgaatt ccaaaatcat gttttaatga atgatgtaat tgttgaatta cctttatgat aagcaaagag ctaatggcat caggcaccat acgcagagga agatgcgttg tgactaatgc cactttcatt tgagaacagg ccaacatcat caccactgtt tccacctcaa agaaatcagc aaaaaactcc gtgtggccag taaacgtaat accggctgca ttaatgtttg ccttatgaac aggcgctgtc actaatgcag aaaactcccc tttggagcat agcgaagccc ctaaagtcaa aagctccata acataagccg cattttgcgg atttaattca ccactaatga ccggtgcagc gcatggaaca ggccatacag ttaaatatcc tgcttttttt ttgaagcttt gatgagggct gtactctaaa aatttaatac tgagatttaa ctcactggct cttgcctcta ataaagacag atcacccaat atcactacgg gcaaatcggt ttctgccaaa gccaggcata agtccgggcc aattccagca ggctctccac tactaataag cagtgggttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN PLLISSGEPA GIGPDLCLAL AETDLPVVIL GDLSLLEARA SELNLSIKFL EYSPHQSFKK KAGYLTVWPV PCAAPVISGE LNPQNAAYVM ELLTLGASLC SKGEFSALVT APVHKANINA AGITFTGHTE FFADFFEVET VVMMLACSQM KVALVTTHLP LRMVPDAISS LLIIKVIQQL HHSLKHDFGI QSPKINVAGL NPHAGESGYL GREEIEIITP ALNTLKNQGI DVLGPLPADT MFITNHINHC DAYVAMYHDQ GLPVLKYAGF NEAVNITLGL PIIRTSVDHG TALELAGKNK ANPGSMLAAV KMAKDMALTR IK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc cactgcttat tagtagtgga gagcctgctg gaattggccc ggacttatgc ctggctttgg cagaaaccga tttgcccgta gtgatattgg gtgatctgtc tttattagag gcaagagcca gtgagttaaa tctcagtatt aaatttttag agtacagccc tcatcaaagc ttcaaaaaaa aagcaggata tttaactgta tggcctgttc catgcgctgc accggtcatt agtggtgaat taaatccgca aaatgcggct tatgttatgg agcttttgac tttaggggct tcgctatgct ccaaagggga gttttctgca ttagtgacag cgcctgttca taaggcaaac attaatgcag ccggtattac gtttactggc cacacggagt tttttgctga tttctttgag gtggaaacag tggtgatgat gttggcctgt tctcaaatga aagtggcatt agtcacaacg catcttcctc tgcgtatggt gcctgatgcc attagctctt tgcttatcat aaaggtaatt caacaattac atcattcatt aaaacatgat tttggaattc aaagtccaaa aatcaacgtg gctggtctga atccgcatgc tggagaatca ggatatttag gtcgagaaga aattgaaatt attactccag cattaaatac tctaaaaaac caggggatcg atgtgttagg gccgttacca gcagatacga tgtttataac aaaccatatc aatcactgtg atgcttatgt ggcaatgtac catgatcaag gactgccagt cttaaagtat gctggattta atgaagcagt aaatataact ctcggattgc cgataattcg tacttctgta gatcatggaa cagcattaga attagctggt aagaacaaag ctaaccctgg gagtatgctt gcggcggtta agatggcgaa ggatatggct ttaacaagga taaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LepnA.00116.a.B1.PS38420
| PURIFICATION DATe: | 3/6/2018 |
| CONCENTRATION: | 29.9mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN PLLISSGEPA GIGPDLCLAL AETDLPVVIL GDLSLLEARA SELNLSIKFL EYSPHQSFKK KAGYLTVWPV PCAAPVISGE LNPQNAAYVM ELLTLGASLC SKGEFSALVT APVHKANINA AGITFTGHTE FFADFFEVET VVMMLACSQM KVALVTTHLP LRMVPDAISS LLIIKVIQQL HHSLKHDFGI QSPKINVAGL NPHAGESGYL GREEIEIITP ALNTLKNQGI DVLGPLPADT MFITNHINHC DAYVAMYHDQ GLPVLKYAGF NEAVNITLGL PIIRTSVDHG TALELAGKNK ANPGSMLAAV KMAKD
Validated NT Sequence
tatccttcgc catcttaacc gccgcaagca tactcccagg gttagctttg ttcttaccag ctaattctaa tgctgttcca tgatctacag aagtacgaat tatcggcaat ccgagagtta tatttactgc ttcattaaat ccagcatact ttaagactgg cagtccttga tcatggtaca ttgccacata agcatcacag tgattgatat ggtttgttat aaacatcgta tctgctggta acggccctaa cacatcgatc ccctggtttt ttagagtatt taatgctgga gtaataattt caatttcttc tcgacctaaa tatcctgatt ctccagcatg cggattcaga ccagccacgt tgatttttgg actttgaatt ccaaaatcat gttttaatga atgatgtaat tgttgaatta cctttatgat aagcaaagag ctaatggcat caggcaccat acgcagagga agatgcgttg tgactaatgc cactttcatt tgagaacagg ccaacatcat caccactgtt tccacctcaa agaaatcagc aaaaaactcc gtgtggccag taaacgtaat accggctgca ttaatgtttg ccttatgaac aggcgctgtc actaatgcag aaaactcccc tttggagcat agcgaagccc ctaaagtcaa aagctccata acataagccg cattttgcgg atttaattca ccactaatga ccggtgcagc gcatggaaca ggccatacag ttaaatatcc tgcttttttt ttgaagcttt gatgagggct gtactctaaa aatttaatac tgagatttaa ctcactggct cttgcctcta ataaagacag atcacccaat atcactacgg gcaaatcggt ttctgccaaa gccaggcata agtccgggcc aattccagca ggctctccac tactaataag cagtgggttc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMN PLLISSGEPA GIGPDLCLAL AETDLPVVIL GDLSLLEARA SELNLSIKFL EYSPHQSFKK KAGYLTVWPV PCAAPVISGE LNPQNAAYVM ELLTLGASLC SKGEFSALVT APVHKANINA AGITFTGHTE FFADFFEVET VVMMLACSQM KVALVTTHLP LRMVPDAISS LLIIKVIQQL HHSLKHDFGI QSPKINVAGL NPHAGESGYL GREEIEIITP ALNTLKNQGI DVLGPLPADT MFITNHINHC DAYVAMYHDQ GLPVLKYAGF NEAVNITLGL PIIRTSVDHG TALELAGKNK ANPGSMLAAV KMAKDMALTR IK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CACTGCTTAT TAGTAGTGGA GAGCCTGCTG GAATTGGCCC GGACTTATGC CTGGCTTTGG CAGAAACCGA TTTGCCCGTA GTGATATTGG GTGATCTGTC TTTATTAGAG GCAAGAGCCA GTGAGTTAAA TCTCAGTATT AAATTTTTAG AGTACAGCCC TCATCAAAGC TTCAAAAAAA AAGCAGGATA TTTAACTGTA TGGCCTGTTC CATGCGCTGC ACCGGTCATT AGTGGTGAAT TAAATCCGCA AAATGCGGCT TATGTTATGG AGCTTTTGAC TTTAGGGGCT TCGCTATGCT CCAAAGGGGA GTTTTCTGCA TTAGTGACAG CGCCTGTTCA TAAGGCAAAC ATTAATGCAG CCGGTATTAC GTTTACTGGC CACACGGAGT TTTTTGCTGA TTTCTTTGAG GTGGAAACAG TGGTGATGAT GTTGGCCTGT TCTCAAATGA AAGTGGCATT AGTCACAACG CATCTTCCTC TGCGTATGGT GCCTGATGCC ATTAGCTCTT TGCTTATCAT AAAGGTAATT CAACAATTAC ATCATTCATT AAAACATGAT TTTGGAATTC AAAGTCCAAA AATCAACGTG GCTGGTCTGA ATCCGCATGC TGGAGAATCA GGATATTTAG GTCGAGAAGA AATTGAAATT ATTACTCCAG CATTAAATAC TCTAAAAAAC CAGGGGATCG ATGTGTTAGG GCCGTTACCA GCAGATACGA TGTTTATAAC AAACCATATC AATCACTGTG ATGCTTATGT GGCAATGTAC CATGATCAAG GACTGCCAGT CTTAAAGTAT GCTGGATTTA ATGAAGCAGT AAATATAACT CTCGGATTGC CGATAATTCG TACTTCTGTA GATCATGGAA CAGCATTAGA ATTAGCTGGT AAGAACAAAG CTAACCCTGG GAGTATGCTT GCGGCGGTTA AGATGGCGAA GGATATGGCT TTAACAAGGA TAAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT