LepnA.00111.a

Glutamine synthetase, type I

CENTER ID: LepnA.00111.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LepnA.00111.a.B1.GE41354 Full length(LepnA.00111.a) 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LepnA.00111.a.B1.PS38375 Full length(LepnA.00111.a) 1 469

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|272624.6.peg.1434
UniProt: Q5ZVS6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKNVVLDAI KEHDAKFVDL RFTDIRGKEQ HITIPVSAVD DDFLENGKMI DGSSFKGWQK IHQSDLALVP DLTSIKPDPF FQDNTLFIRC NVVDPQTMMG YDRCPRSIAQ RAENYLQSTG IADGVNFGPE PEFFLFDNVQ WETTISGSFY KIDSDEAHWN SGKEMEGGNI GHRPPLKGGY FPVPPVDSSH DIRSAICITL ESLGMVVEAH HHEVATANQC EVATRFNTLT KKADELQILK YVIHNVAHNY GKTATFMPKP LVGDNGSGMH CHQSLYKDGY NLFSGDQYSG LSETALYYIG GIIKHARALN AFTNPSTNSY KRLVPGFEAP VLLAYSARNR SAAIRIPHVS NPKARRIEVR FPDPTANPYL AFSAMMMAGI DGIQKKIYPG QPMDKDLYDL PPEELVDVPT VCSSLEMAMD HLRADHEFLL QGDVFSRDFI NNYIQMKEEE ITKIRSLTHP YEFELYYSL
NT Sequence
ATGACAAAAA ATGTAGTGCT TGATGCAATC AAAGAACATG ACGCCAAATT TGTGGATTTA AGATTTACAG ATATCCGTGG AAAAGAGCAA CACATTACCA TACCTGTTTC AGCAGTTGAT GATGATTTTC TTGAGAATGG AAAAATGATC GATGGCTCAT CTTTTAAAGG TTGGCAGAAA ATTCATCAAT CAGATTTGGC TTTAGTGCCA GATTTAACTT CAATTAAGCC GGATCCATTT TTTCAGGACA ACACTTTATT TATACGATGT AATGTAGTTG ATCCACAGAC TATGATGGGT TATGACAGAT GCCCACGTTC AATCGCACAA CGCGCAGAAA ATTATTTACA ATCTACAGGG ATTGCAGATG GTGTAAATTT TGGCCCTGAG CCTGAGTTTT TTCTCTTCGA CAATGTGCAA TGGGAAACTA CTATCAGCGG TTCATTTTAT AAAATAGATT CAGACGAGGC ACATTGGAAC TCCGGTAAGG AAATGGAAGG CGGAAATATT GGCCATAGAC CTCCGTTGAA AGGCGGTTAT TTCCCTGTTC CTCCAGTCGA TTCATCTCAT GATATTCGTT CAGCAATTTG TATCACATTG GAATCTTTAG GAATGGTTGT GGAAGCACAT CATCATGAAG TAGCTACAGC TAACCAGTGT GAAGTAGCTA CCCGTTTTAA TACACTCACC AAAAAGGCAG ACGAACTACA AATTCTGAAA TACGTGATAC ATAATGTTGC CCATAACTAT GGGAAAACCG CTACTTTTAT GCCAAAACCT TTGGTAGGAG ATAATGGAAG CGGGATGCAC TGTCATCAAT CACTTTATAA AGATGGGTAT AATCTGTTTT CCGGTGATCA GTATTCGGGG CTTTCTGAAA CAGCTTTATA TTACATAGGT GGAATAATCA AGCATGCCAG AGCTTTAAAC GCTTTTACCA ATCCGTCAAC CAATAGCTAT AAGAGATTAG TTCCCGGGTT TGAAGCGCCT GTATTACTGG CTTACTCAGC AAGAAATCGC TCCGCCGCTA TCCGTATCCC ACACGTTAGC AATCCAAAGG CACGACGTAT AGAAGTACGT TTCCCTGATC CAACAGCTAA TCCTTATCTT GCTTTCTCAG CAATGATGAT GGCAGGCATA GATGGCATTC AGAAAAAAAT CTACCCTGGG CAGCCAATGG ATAAAGATCT TTATGATTTA CCGCCTGAAG AGCTTGTTGA TGTACCCACT GTTTGCTCTT CATTAGAGAT GGCTATGGAC CATTTGCGGG CAGATCATGA ATTTTTGTTA CAGGGAGATG TGTTCAGCAG AGACTTTATT AATAATTATA TTCAAATGAA AGAAGAGGAA ATTACTAAAA TCAGGAGCTT GACTCATCCT TATGAATTTG AGTTATATTA TAGTCTC
Details for LepnA.00111.a.B1.GE41354
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 54kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MTKNVVLDAI KEHDAKFVDL RFTDIRGKEQ HITIPVSAVD DDFLENGKMI DGSSFKGWQK IHQSDLALVP DLTSIKPDPF FQDNTLFIRC NVVDPQTMMG YDRCPRSIAQ RAENYLQSTG IADGVNFGPE PEFFLFDNVQ WETTISGSFY KIDSDEAHWN SGKEMEGGNI GHRPPLKGGY FPVPPVDSSH DIRSAICITL ESLGMVVEAH HHEVATANQC EVATRFNTLT KKADELQILK YVIHNVAHNY GKTATFMPKP LVGDNGSGMH CHQSLYKDGY NLFSGDQYSG LSETALYYIG GIIKHARALN AFTNPSTNSY KRLVPGFEAP VLLAYSARNR SAAIRIPHVS NPKARRIEVR FPDPTANPYL AFSAMMMAGI DGIQKKIYPG QPMDKDLYDL PPEELVDVPT VCSSLEMAMD HLRADHEFLL QGDVFSRDFI NNYIQMKEEE ITKIRSLTHP YE
Validated NT Sequence
attcataagg atgagtcaag ctcctgattt tagtaatttc ctcttctttc atttgaatat aattattaat aaagtctctg ctgaacacat ctccctgtaa caaaaattca tgatctgccc gcaaatggtc catagccatc tctaatgaag agcaaacagt gggtacatca acaagctctt caggcggtaa atcataaaga tctttatcca ttggctgccc agggtagatt tttttctgaa tgccatctat gcctgccatc atcattgctg agaaagcaag ataaggatta gctgttggat cagggaaacg tacttctata cgtcgtgcct ttggattgct aacgtgtggg atacggatag cggcggagcg atttcttgct gagtaagcca gtaatacagg cgcttcaaac ccgggaacta atctcttata gctattggtt gacggattgg taaaagcgtt taaagctctg gcatgcttga ttattccacc tatgtaatat aaagctgttt cagaaagccc cgaatactga tcaccggaaa acagattata cccatcttta taaagtgatt gatgacagtg catcccgctt ccattatctc ctaccaaagg ttttggcata aaagtagcgg ttttcccata gttatgggca acattatgta tcacgtattt cagaatttgt agttcgtctg cctttttggt gagtgtatta aaacgggtag ctacttcaca ctggttagct gtagctactt catgatgatg tgcttccaca accattccta aagattccaa tgtgatacaa attgctgaac gaatatcatg agatgaatcg actggaggaa cagggaaata accgcctttc aacggaggtc tatggccaat atttccgcct tccatttcct taccggagtt ccaatgtgcc tcgtctgaat ctattttata aaatgaaccg ctgatagtag tttcccattg cacattgtcg aagagaaaaa actcaggctc agggccaaaa tttacaccat ctgcaatccc tgtagattgt aaataatttt ctgcgcgttg tgcgattgaa cgtgggcatc tgtcataacc catcatagtc tgtggatcaa ctacattaca tcgtataaat aaagtgttgt cctgaaaaaa tggatccggc ttaattgaag ttaaatctgg cactaaagcc aaatctgatt gatgaatttt ctgccaacct ttaaaagatg agccatcgat catttttcca ttctcaagaa aatcatcatc aactgctgaa acaggtatgg taatgtgttg ctcttttcca cggatatctg taaatcttaa atccacaaat ttggcgtcat gttctttgat tgcatcaagc actacatttt ttgtcatatg gtggtggtgg tggtga
Expected Protein Sequence
MAHHHHHHMT KNVVLDAIKE HDAKFVDLRF TDIRGKEQHI TIPVSAVDDD FLENGKMIDG SSFKGWQKIH QSDLALVPDL TSIKPDPFFQ DNTLFIRCNV VDPQTMMGYD RCPRSIAQRA ENYLQSTGIA DGVNFGPEPE FFLFDNVQWE TTISGSFYKI DSDEAHWNSG KEMEGGNIGH RPPLKGGYFP VPPVDSSHDI RSAICITLES LGMVVEAHHH EVATANQCEV ATRFNTLTKK ADELQILKYV IHNVAHNYGK TATFMPKPLV GDNGSGMHCH QSLYKDGYNL FSGDQYSGLS ETALYYIGGI IKHARALNAF TNPSTNSYKR LVPGFEAPVL LAYSARNRSA AIRIPHVSNP KARRIEVRFP DPTANPYLAF SAMMMAGIDG IQKKIYPGQP MDKDLYDLPP EELVDVPTVC SSLEMAMDHL RADHEFLLQG DVFSRDFINN YIQMKEEEIT KIRSLTHPYE FELYYSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa aaaatgtagt gcttgatgca atcaaagaac atgacgccaa atttgtggat ttaagattta cagatatccg tggaaaagag caacacatta ccatacctgt ttcagcagtt gatgatgatt ttcttgagaa tggaaaaatg atcgatggct catcttttaa aggttggcag aaaattcatc aatcagattt ggctttagtg ccagatttaa cttcaattaa gccggatcca ttttttcagg acaacacttt atttatacga tgtaatgtag ttgatccaca gactatgatg ggttatgaca gatgcccacg ttcaatcgca caacgcgcag aaaattattt acaatctaca gggattgcag atggtgtaaa ttttggccct gagcctgagt tttttctctt cgacaatgtg caatgggaaa ctactatcag cggttcattt tataaaatag attcagacga ggcacattgg aactccggta aggaaatgga aggcggaaat attggccata gacctccgtt gaaaggcggt tatttccctg ttcctccagt cgattcatct catgatattc gttcagcaat ttgtatcaca ttggaatctt taggaatggt tgtggaagca catcatcatg aagtagctac agctaaccag tgtgaagtag ctacccgttt taatacactc accaaaaagg cagacgaact acaaattctg aaatacgtga tacataatgt tgcccataac tatgggaaaa ccgctacttt tatgccaaaa cctttggtag gagataatgg aagcgggatg cactgtcatc aatcacttta taaagatggg tataatctgt tttccggtga tcagtattcg gggctttctg aaacagcttt atattacata ggtggaataa tcaagcatgc cagagcttta aacgctttta ccaatccgtc aaccaatagc tataagagat tagttcccgg gtttgaagcg cctgtattac tggcttactc agcaagaaat cgctccgccg ctatccgtat cccacacgtt agcaatccaa aggcacgacg tatagaagta cgtttccctg atccaacagc taatccttat cttgctttct cagcaatgat gatggcaggc atagatggca ttcagaaaaa aatctaccct gggcagccaa tggataaaga tctttatgat ttaccgcctg aagagcttgt tgatgtaccc actgtttgct cttcattaga gatggctatg gaccatttgc gggcagatca tgaatttttg ttacagggag atgtgttcag cagagacttt attaataatt atattcaaat gaaagaagag gaaattacta aaatcaggag cttgactcat ccttatgaat ttgagttata ttatagtctc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LepnA.00111.a.B1.PS38375
PURIFICATION DATe: 11/9/2017
CONCENTRATION: 6.7mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MTKNVVLDAI KEHDAKFVDL RFTDIRGKEQ HITIPVSAVD DDFLENGKMI DGSSFKGWQK IHQSDLALVP DLTSIKPDPF FQDNTLFIRC NVVDPQTMMG YDRCPRSIAQ RAENYLQSTG IADGVNFGPE PEFFLFDNVQ WETTISGSFY KIDSDEAHWN SGKEMEGGNI GHRPPLKGGY FPVPPVDSSH DIRSAICITL ESLGMVVEAH HHEVATANQC EVATRFNTLT KKADELQILK YVIHNVAHNY GKTATFMPKP LVGDNGSGMH CHQSLYKDGY NLFSGDQYSG LSETALYYIG GIIKHARALN AFTNPSTNSY KRLVPGFEAP VLLAYSARNR SAAIRIPHVS NPKARRIEVR FPDPTANPYL AFSAMMMAGI DGIQKKIYPG QPMDKDLYDL PPEELVDVPT VCSSLEMAMD HLRADHEFLL QGDVFSRDFI NNYIQMKEEE ITKIRSLTHP YE
Validated NT Sequence
attcataagg atgagtcaag ctcctgattt tagtaatttc ctcttctttc atttgaatat aattattaat aaagtctctg ctgaacacat ctccctgtaa caaaaattca tgatctgccc gcaaatggtc catagccatc tctaatgaag agcaaacagt gggtacatca acaagctctt caggcggtaa atcataaaga tctttatcca ttggctgccc agggtagatt tttttctgaa tgccatctat gcctgccatc atcattgctg agaaagcaag ataaggatta gctgttggat cagggaaacg tacttctata cgtcgtgcct ttggattgct aacgtgtggg atacggatag cggcggagcg atttcttgct gagtaagcca gtaatacagg cgcttcaaac ccgggaacta atctcttata gctattggtt gacggattgg taaaagcgtt taaagctctg gcatgcttga ttattccacc tatgtaatat aaagctgttt cagaaagccc cgaatactga tcaccggaaa acagattata cccatcttta taaagtgatt gatgacagtg catcccgctt ccattatctc ctaccaaagg ttttggcata aaagtagcgg ttttcccata gttatgggca acattatgta tcacgtattt cagaatttgt agttcgtctg cctttttggt gagtgtatta aaacgggtag ctacttcaca ctggttagct gtagctactt catgatgatg tgcttccaca accattccta aagattccaa tgtgatacaa attgctgaac gaatatcatg agatgaatcg actggaggaa cagggaaata accgcctttc aacggaggtc tatggccaat atttccgcct tccatttcct taccggagtt ccaatgtgcc tcgtctgaat ctattttata aaatgaaccg ctgatagtag tttcccattg cacattgtcg aagagaaaaa actcaggctc agggccaaaa tttacaccat ctgcaatccc tgtagattgt aaataatttt ctgcgcgttg tgcgattgaa cgtgggcatc tgtcataacc catcatagtc tgtggatcaa ctacattaca tcgtataaat aaagtgttgt cctgaaaaaa tggatccggc ttaattgaag ttaaatctgg cactaaagcc aaatctgatt gatgaatttt ctgccaacct ttaaaagatg agccatcgat catttttcca ttctcaagaa aatcatcatc aactgctgaa acaggtatgg taatgtgttg ctcttttcca cggatatctg taaatcttaa atccacaaat ttggcgtcat gttctttgat tgcatcaagc actacatttt ttgtcatatg gtggtggtgg tggtga
Expressed Protein Sequence
MAHHHHHHMT KNVVLDAIKE HDAKFVDLRF TDIRGKEQHI TIPVSAVDDD FLENGKMIDG SSFKGWQKIH QSDLALVPDL TSIKPDPFFQ DNTLFIRCNV VDPQTMMGYD RCPRSIAQRA ENYLQSTGIA DGVNFGPEPE FFLFDNVQWE TTISGSFYKI DSDEAHWNSG KEMEGGNIGH RPPLKGGYFP VPPVDSSHDI RSAICITLES LGMVVEAHHH EVATANQCEV ATRFNTLTKK ADELQILKYV IHNVAHNYGK TATFMPKPLV GDNGSGMHCH QSLYKDGYNL FSGDQYSGLS ETALYYIGGI IKHARALNAF TNPSTNSYKR LVPGFEAPVL LAYSARNRSA AIRIPHVSNP KARRIEVRFP DPTANPYLAF SAMMMAGIDG IQKKIYPGQP MDKDLYDLPP EELVDVPTVC SSLEMAMDHL RADHEFLLQG DVFSRDFINN YIQMKEEEIT KIRSLTHPYE FELYYSL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA AAAATGTAGT GCTTGATGCA ATCAAAGAAC ATGACGCCAA ATTTGTGGAT TTAAGATTTA CAGATATCCG TGGAAAAGAG CAACACATTA CCATACCTGT TTCAGCAGTT GATGATGATT TTCTTGAGAA TGGAAAAATG ATCGATGGCT CATCTTTTAA AGGTTGGCAG AAAATTCATC AATCAGATTT GGCTTTAGTG CCAGATTTAA CTTCAATTAA GCCGGATCCA TTTTTTCAGG ACAACACTTT ATTTATACGA TGTAATGTAG TTGATCCACA GACTATGATG GGTTATGACA GATGCCCACG TTCAATCGCA CAACGCGCAG AAAATTATTT ACAATCTACA GGGATTGCAG ATGGTGTAAA TTTTGGCCCT GAGCCTGAGT TTTTTCTCTT CGACAATGTG CAATGGGAAA CTACTATCAG CGGTTCATTT TATAAAATAG ATTCAGACGA GGCACATTGG AACTCCGGTA AGGAAATGGA AGGCGGAAAT ATTGGCCATA GACCTCCGTT GAAAGGCGGT TATTTCCCTG TTCCTCCAGT CGATTCATCT CATGATATTC GTTCAGCAAT TTGTATCACA TTGGAATCTT TAGGAATGGT TGTGGAAGCA CATCATCATG AAGTAGCTAC AGCTAACCAG TGTGAAGTAG CTACCCGTTT TAATACACTC ACCAAAAAGG CAGACGAACT ACAAATTCTG AAATACGTGA TACATAATGT TGCCCATAAC TATGGGAAAA CCGCTACTTT TATGCCAAAA CCTTTGGTAG GAGATAATGG AAGCGGGATG CACTGTCATC AATCACTTTA TAAAGATGGG TATAATCTGT TTTCCGGTGA TCAGTATTCG GGGCTTTCTG AAACAGCTTT ATATTACATA GGTGGAATAA TCAAGCATGC CAGAGCTTTA AACGCTTTTA CCAATCCGTC AACCAATAGC TATAAGAGAT TAGTTCCCGG GTTTGAAGCG CCTGTATTAC TGGCTTACTC AGCAAGAAAT CGCTCCGCCG CTATCCGTAT CCCACACGTT AGCAATCCAA AGGCACGACG TATAGAAGTA CGTTTCCCTG ATCCAACAGC TAATCCTTAT CTTGCTTTCT CAGCAATGAT GATGGCAGGC ATAGATGGCA TTCAGAAAAA AATCTACCCT GGGCAGCCAA TGGATAAAGA TCTTTATGAT TTACCGCCTG AAGAGCTTGT TGATGTACCC ACTGTTTGCT CTTCATTAGA GATGGCTATG GACCATTTGC GGGCAGATCA TGAATTTTTG TTACAGGGAG ATGTGTTCAG CAGAGACTTT ATTAATAATT ATATTCAAAT GAAAGAAGAG GAAATTACTA AAATCAGGAG CTTGACTCAT CCTTATGAAT TTGAGTTATA TTATAGTCTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT