CENTER ID: | LepnA.00111.a |
ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
ASSOCIATED DISEASE: | |
CURRENT STATUS: | diffraction |
COMMUNITY REQUEST: | False |
NIH RISK GROUP: | |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LepnA.00111.a.B1.GE41354 | Full length(LepnA.00111.a) | 1 | 469 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
LepnA.00111.a.B1.PS38375 | Full length(LepnA.00111.a) | 1 | 469 |
RESOURCE | REFERENCE ID |
---|---|
PATRIC ID: | fig|272624.6.peg.1434 |
UniProt: | Q5ZVS6 |
HARVESTED ON: | 4/3/2017 |
SEQUENCED ON: | 4/10/2017 |
EXPECTED MW: | 54kDa |
OBSERVED MW: | 56kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Many (50-100) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 96 |
PURIFICATION DATe: | 11/9/2017 |
CONCENTRATION: | 6.7mg/ml |
OBSERVED MW: | 64kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 3 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 96 |