LepnA.00092.a
Isocitrate dehydrogenase [NADP]
| CENTER ID: | LepnA.00092.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00092.a.B1.GE41351 | Full length(LepnA.00092.a) | 1 | 422 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00092.a.B1.PW38312 | Full length(LepnA.00092.a) | 1 | 422 |
Structures
6C0E
DEPOSITED: 12/29/2017
DETERMINATION: XRay
CLONE: LepnA.00092.a.B1.GE41351
PROTEIN: LepnA.00092.a.B1.PW38312
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|272624.6.peg.845 |
| UniProt: | Q5ZXB6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MESSMTYDKI KVPAQGEAIT VAADHSLHVP DNPIIPFIEG DGIGVDVTPP MIRVVDAAVQ KAYGNKRKIS WMEVYAGEKA TKVYGGDQWL PKETLDAMKK YVVSIKGPLT TPVGGGIRSL NVAIRQDMDL YVCLRPIRYF NGVPSPVREP WKTDMVIFRE NSEDIYAGIE WQADTPEAKK VIQFLTKEMG VKKIRFPEHC GIGVKPVSRE GTTRLVKAAI QYAIDNDRST VTLVHKGNIM KFTEGAFKDW GYQVARDSFG AKEYQGGPWM EFKNPKTGKQ IIINDVIADA FLQQILLRPE DYSVIATLNL NGDYISDALA AQVGGIGIAP GANISDQMAV FEATHGTAPK YAGQNKVNPG SIILSAEMML RHMGWYEAAD LIIRGMEGAI EAKTVTYDFE RGMQGATLVS SSGFADAMIK HM
NT Sequence
ATGGAGTCGT CAATGACATA CGATAAAATC AAAGTGCCTG CCCAAGGCGA AGCTATTACT GTTGCTGCTG ACCATTCACT TCATGTTCCT GATAATCCAA TTATTCCTTT TATTGAGGGT GATGGTATCG GTGTTGATGT AACACCTCCG ATGATCCGTG TTGTTGATGC TGCAGTTCAA AAAGCATATG GCAACAAGAG AAAAATTTCC TGGATGGAGG TTTATGCCGG GGAAAAGGCT ACTAAGGTTT ATGGTGGCGA TCAATGGCTT CCCAAAGAGA CTCTGGATGC CATGAAAAAA TATGTGGTTT CAATTAAAGG TCCTTTAACT ACTCCTGTTG GTGGCGGTAT ACGTTCATTG AATGTCGCTA TTCGCCAAGA CATGGATCTC TATGTTTGTT TACGTCCAAT TCGCTATTTT AATGGTGTTC CAAGTCCGGT TCGGGAGCCA TGGAAAACCG ATATGGTCAT TTTCAGGGAA AATTCTGAAG ATATTTATGC CGGTATTGAA TGGCAGGCAG ATACCCCGGA AGCTAAAAAA GTGATTCAAT TTTTAACTAA GGAAATGGGT GTTAAGAAAA TTCGTTTTCC TGAGCATTGT GGTATAGGTG TGAAGCCTGT TTCCAGAGAA GGTACCACTC GACTTGTCAA AGCGGCCATT CAGTATGCGA TAGATAATGA CCGCAGTACA GTGACTTTGG TACATAAAGG CAATATCATG AAGTTTACTG AAGGGGCTTT TAAAGATTGG GGATATCAAG TTGCTCGCGA TTCTTTTGGA GCAAAAGAAT ATCAAGGTGG GCCATGGATG GAATTTAAAA ATCCTAAAAC CGGTAAGCAG ATTATCATTA ATGATGTGAT CGCTGATGCC TTTTTGCAAC AAATCCTGTT AAGACCTGAA GACTATAGTG TCATTGCTAC ATTAAATTTG AATGGTGATT ATATTTCTGA TGCTTTGGCT GCTCAGGTTG GTGGAATAGG TATAGCTCCC GGAGCGAACA TTAGTGATCA AATGGCTGTG TTTGAAGCAA CTCATGGAAC TGCGCCTAAA TATGCTGGGC AGAATAAAGT GAACCCCGGT TCTATTATTC TTTCTGCCGA AATGATGTTA CGTCATATGG GGTGGTATGA AGCAGCTGAT TTGATTATCA GAGGTATGGA AGGCGCGATA GAAGCCAAAA CCGTAACTTA TGATTTTGAA CGTGGTATGC AAGGAGCAAC GTTAGTCAGC AGCTCTGGTT TTGCAGATGC CATGATTAAG CATATG
Details for LepnA.00092.a.B1.GE41351
| HARVESTED ON: | 4/3/2017 |
| SEQUENCED ON: | 4/10/2017 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHME SSMTYDKIKV PAQGEAITVA ADHSLHVPDN PIIPFIEGDG IGVDVTPPMI RVVDAAVQKA YGNKRKISWM EVYAGEKATK VYGGDQWLPK ETLDAMKKYV VSIKGPLTTP VGGGIRSLNV AIRQDMDLYV CLRPIRYFNG VPSPVREPWK TDMVIFRENS EDIYAGIEWQ ADTPEAKKVI QFLTKEMGVK KIRFPEHCGI GVKPVSREGT TRLVKAAIQY AIDNDRSTVT LVHKGNIMKF TEGAFKDWGY QVARDSFGAK EYQGGPWMEF KNPKTGKQII INDVIADAFL QQILLRPEDY SVIATLNLNG DYISDALAAQ VGGIGIAPGA NISDQMAVFE ATHGTAPKYA GQNKVNPGSI ILSAEMMLRH MGWYEAADLI IRGMEGAIEA KTVTYDFERG MQGATLVSSS GFA
Validated NT Sequence
ctgcaaaacc agagctgctg actaacgttg ctccttgcat accacgttca aaatcataag ttacggtttt ggcttctatc gcgccttcca tacctctgat aatcaaatca gctgcttcat accaccccat atgacgtaac atcatttcgg cagaaagaat aatagaaccg gggttcactt tattctgccc agcatattta ggcgcagttc catgagttgc ttcaaacaca gccatttgat cactaatgtt cgctccggga gctataccta ttccaccaac ctgagcagcc aaagcatcag aaatataatc accattcaaa tttaatgtag caatgacact atagtcttca ggtcttaaca ggatttgttg caaaaaggca tcagcgatca catcattaat gataatctgc ttaccggttt taggattttt aaattccatc catggcccac cttgatattc ttttgctcca aaagaatcgc gagcaacttg atatccccaa tctttaaaag ccccttcagt aaacttcatg atattgcctt tatgtaccaa agtcactgta ctgcggtcat tatctatcgc atactgaatg gccgctttga caagtcgagt ggtaccttct ctggaaacag gcttcacacc tataccacaa tgctcaggaa aacgaatttt cttaacaccc atttccttag ttaaaaattg aatcactttt ttagcttccg gggtatctgc ctgccattca ataccggcat aaatatcttc agaattttcc ctgaaaatga ccatatcggt tttccatggc tcccgaaccg gacttggaac accattaaaa tagcgaattg gacgtaaaca aacatagaga tccatgtctt ggcgaatagc gacattcaat gaacgtatac cgccaccaac aggagtagtt aaaggacctt taattgaaac cacatatttt ttcatggcat ccagagtctc tttgggaagc cattgatcgc caccataaac cttagtagcc ttttccccgg cataaacctc catccaggaa atttttctct tgttgccata tgctttttga actgcagcat caacaacacg gatcatcgga ggtgttacat caacaccgat accatcaccc tcaataaaag gaataattgg attatcagga acatgaagtg aatggtcagc agcaacagta atagcttcgc cttgggcagg cactttgatt ttatcgtatg tcattgacga ctccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHME SSMTYDKIKV PAQGEAITVA ADHSLHVPDN PIIPFIEGDG IGVDVTPPMI RVVDAAVQKA YGNKRKISWM EVYAGEKATK VYGGDQWLPK ETLDAMKKYV VSIKGPLTTP VGGGIRSLNV AIRQDMDLYV CLRPIRYFNG VPSPVREPWK TDMVIFRENS EDIYAGIEWQ ADTPEAKKVI QFLTKEMGVK KIRFPEHCGI GVKPVSREGT TRLVKAAIQY AIDNDRSTVT LVHKGNIMKF TEGAFKDWGY QVARDSFGAK EYQGGPWMEF KNPKTGKQII INDVIADAFL QQILLRPEDY SVIATLNLNG DYISDALAAQ VGGIGIAPGA NISDQMAVFE ATHGTAPKYA GQNKVNPGSI ILSAEMMLRH MGWYEAADLI IRGMEGAIEA KTVTYDFERG MQGATLVSSS GFADAMIKHM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagt cgtcaatgac atacgataaa atcaaagtgc ctgcccaagg cgaagctatt actgttgctg ctgaccattc acttcatgtt cctgataatc caattattcc ttttattgag ggtgatggta tcggtgttga tgtaacacct ccgatgatcc gtgttgttga tgctgcagtt caaaaagcat atggcaacaa gagaaaaatt tcctggatgg aggtttatgc cggggaaaag gctactaagg tttatggtgg cgatcaatgg cttcccaaag agactctgga tgccatgaaa aaatatgtgg tttcaattaa aggtccttta actactcctg ttggtggcgg tatacgttca ttgaatgtcg ctattcgcca agacatggat ctctatgttt gtttacgtcc aattcgctat tttaatggtg ttccaagtcc ggttcgggag ccatggaaaa ccgatatggt cattttcagg gaaaattctg aagatattta tgccggtatt gaatggcagg cagatacccc ggaagctaaa aaagtgattc aatttttaac taaggaaatg ggtgttaaga aaattcgttt tcctgagcat tgtggtatag gtgtgaagcc tgtttccaga gaaggtacca ctcgacttgt caaagcggcc attcagtatg cgatagataa tgaccgcagt acagtgactt tggtacataa aggcaatatc atgaagttta ctgaaggggc ttttaaagat tggggatatc aagttgctcg cgattctttt ggagcaaaag aatatcaagg tgggccatgg atggaattta aaaatcctaa aaccggtaag cagattatca ttaatgatgt gatcgctgat gcctttttgc aacaaatcct gttaagacct gaagactata gtgtcattgc tacattaaat ttgaatggtg attatatttc tgatgctttg gctgctcagg ttggtggaat aggtatagct cccggagcga acattagtga tcaaatggct gtgtttgaag caactcatgg aactgcgcct aaatatgctg ggcagaataa agtgaacccc ggttctatta ttctttctgc cgaaatgatg ttacgtcata tggggtggta tgaagcagct gatttgatta tcagaggtat ggaaggcgcg atagaagcca aaaccgtaac ttatgatttt gaacgtggta tgcaaggagc aacgttagtc agcagctctg gttttgcaga tgccatgatt aagcatatgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LepnA.00092.a.B1.PW38312
| PURIFICATION DATe: | 7/17/2017 |
| CONCENTRATION: | 47.44mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
Followed UWPPG uncleaved ver 1.0 protocol
Validated AA Sequence
MAHHHHHHME SSMTYDKIKV PAQGEAITVA ADHSLHVPDN PIIPFIEGDG IGVDVTPPMI RVVDAAVQKA YGNKRKISWM EVYAGEKATK VYGGDQWLPK ETLDAMKKYV VSIKGPLTTP VGGGIRSLNV AIRQDMDLYV CLRPIRYFNG VPSPVREPWK TDMVIFRENS EDIYAGIEWQ ADTPEAKKVI QFLTKEMGVK KIRFPEHCGI GVKPVSREGT TRLVKAAIQY AIDNDRSTVT LVHKGNIMKF TEGAFKDWGY QVARDSFGAK EYQGGPWMEF KNPKTGKQII INDVIADAFL QQILLRPEDY SVIATLNLNG DYISDALAAQ VGGIGIAPGA NISDQMAVFE ATHGTAPKYA GQNKVNPGSI ILSAEMMLRH MGWYEAADLI IRGMEGAIEA KTVTYDFERG MQGATLVSSS GFA
Validated NT Sequence
ctgcaaaacc agagctgctg actaacgttg ctccttgcat accacgttca aaatcataag ttacggtttt ggcttctatc gcgccttcca tacctctgat aatcaaatca gctgcttcat accaccccat atgacgtaac atcatttcgg cagaaagaat aatagaaccg gggttcactt tattctgccc agcatattta ggcgcagttc catgagttgc ttcaaacaca gccatttgat cactaatgtt cgctccggga gctataccta ttccaccaac ctgagcagcc aaagcatcag aaatataatc accattcaaa tttaatgtag caatgacact atagtcttca ggtcttaaca ggatttgttg caaaaaggca tcagcgatca catcattaat gataatctgc ttaccggttt taggattttt aaattccatc catggcccac cttgatattc ttttgctcca aaagaatcgc gagcaacttg atatccccaa tctttaaaag ccccttcagt aaacttcatg atattgcctt tatgtaccaa agtcactgta ctgcggtcat tatctatcgc atactgaatg gccgctttga caagtcgagt ggtaccttct ctggaaacag gcttcacacc tataccacaa tgctcaggaa aacgaatttt cttaacaccc atttccttag ttaaaaattg aatcactttt ttagcttccg gggtatctgc ctgccattca ataccggcat aaatatcttc agaattttcc ctgaaaatga ccatatcggt tttccatggc tcccgaaccg gacttggaac accattaaaa tagcgaattg gacgtaaaca aacatagaga tccatgtctt ggcgaatagc gacattcaat gaacgtatac cgccaccaac aggagtagtt aaaggacctt taattgaaac cacatatttt ttcatggcat ccagagtctc tttgggaagc cattgatcgc caccataaac cttagtagcc ttttccccgg cataaacctc catccaggaa atttttctct tgttgccata tgctttttga actgcagcat caacaacacg gatcatcgga ggtgttacat caacaccgat accatcaccc tcaataaaag gaataattgg attatcagga acatgaagtg aatggtcagc agcaacagta atagcttcgc cttgggcagg cactttgatt ttatcgtatg tcattgacga ctccatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHME SSMTYDKIKV PAQGEAITVA ADHSLHVPDN PIIPFIEGDG IGVDVTPPMI RVVDAAVQKA YGNKRKISWM EVYAGEKATK VYGGDQWLPK ETLDAMKKYV VSIKGPLTTP VGGGIRSLNV AIRQDMDLYV CLRPIRYFNG VPSPVREPWK TDMVIFRENS EDIYAGIEWQ ADTPEAKKVI QFLTKEMGVK KIRFPEHCGI GVKPVSREGT TRLVKAAIQY AIDNDRSTVT LVHKGNIMKF TEGAFKDWGY QVARDSFGAK EYQGGPWMEF KNPKTGKQII INDVIADAFL QQILLRPEDY SVIATLNLNG DYISDALAAQ VGGIGIAPGA NISDQMAVFE ATHGTAPKYA GQNKVNPGSI ILSAEMMLRH MGWYEAADLI IRGMEGAIEA KTVTYDFERG MQGATLVSSS GFADAMIKHM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAGT CGTCAATGAC ATACGATAAA ATCAAAGTGC CTGCCCAAGG CGAAGCTATT ACTGTTGCTG CTGACCATTC ACTTCATGTT CCTGATAATC CAATTATTCC TTTTATTGAG GGTGATGGTA TCGGTGTTGA TGTAACACCT CCGATGATCC GTGTTGTTGA TGCTGCAGTT CAAAAAGCAT ATGGCAACAA GAGAAAAATT TCCTGGATGG AGGTTTATGC CGGGGAAAAG GCTACTAAGG TTTATGGTGG CGATCAATGG CTTCCCAAAG AGACTCTGGA TGCCATGAAA AAATATGTGG TTTCAATTAA AGGTCCTTTA ACTACTCCTG TTGGTGGCGG TATACGTTCA TTGAATGTCG CTATTCGCCA AGACATGGAT CTCTATGTTT GTTTACGTCC AATTCGCTAT TTTAATGGTG TTCCAAGTCC GGTTCGGGAG CCATGGAAAA CCGATATGGT CATTTTCAGG GAAAATTCTG AAGATATTTA TGCCGGTATT GAATGGCAGG CAGATACCCC GGAAGCTAAA AAAGTGATTC AATTTTTAAC TAAGGAAATG GGTGTTAAGA AAATTCGTTT TCCTGAGCAT TGTGGTATAG GTGTGAAGCC TGTTTCCAGA GAAGGTACCA CTCGACTTGT CAAAGCGGCC ATTCAGTATG CGATAGATAA TGACCGCAGT ACAGTGACTT TGGTACATAA AGGCAATATC ATGAAGTTTA CTGAAGGGGC TTTTAAAGAT TGGGGATATC AAGTTGCTCG CGATTCTTTT GGAGCAAAAG AATATCAAGG TGGGCCATGG ATGGAATTTA AAAATCCTAA AACCGGTAAG CAGATTATCA TTAATGATGT GATCGCTGAT GCCTTTTTGC AACAAATCCT GTTAAGACCT GAAGACTATA GTGTCATTGC TACATTAAAT TTGAATGGTG ATTATATTTC TGATGCTTTG GCTGCTCAGG TTGGTGGAAT AGGTATAGCT CCCGGAGCGA ACATTAGTGA TCAAATGGCT GTGTTTGAAG CAACTCATGG AACTGCGCCT AAATATGCTG GGCAGAATAA AGTGAACCCC GGTTCTATTA TTCTTTCTGC CGAAATGATG TTACGTCATA TGGGGTGGTA TGAAGCAGCT GATTTGATTA TCAGAGGTAT GGAAGGCGCG ATAGAAGCCA AAACCGTAAC TTATGATTTT GAACGTGGTA TGCAAGGAGC AACGTTAGTC AGCAGCTCTG GTTTTGCAGA TGCCATGATT AAGCATATGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT