LepnA.00036.a

Acetyl CoA carboxylase, biotin carboxylase subunit

CENTER ID: LepnA.00036.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00036.a.B1.GE41294 Full length(LepnA.00036.a) 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00036.a.B1.PW38292 Full length(LepnA.00036.a) 1 449
LepnA.00036.a.B1.PW38293 Full length(LepnA.00036.a) 1 449
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272624.6.peg.478
UniProt: Q5ZYB0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFMLSKIVIA NRGEIALRIL RACKELGIQT VAVHSDVDKD LLHVRLADET VCIGPAPAQK SYLNIPAIIS AAEITDAVAI HPGYGFLSEN ADFADIVEQS GFRFIGPRGD SIRLMGDKVS AIAAMKAAGV PCVPGSDGPL GDDDNKNLEL ARKIGYPIII KAAGGGGGRG MRVVHSESNL LSAIALTRSE AKAAFNNPIV YMEKFLENPR HVEFQVLGDG KGHAIHLGER DCSMQRRHQK VVEEAPAPGI TPELREEIGA SVIKACKELQ YRGAGTFEFL FQDGCFYFIE MNTRIQVEHP VTEMITGIDL IKEQIKIASD IPLTLTQKDI KLRGHAIECR INAEDPRNFM PSPGTIKLLH QPGGPGIRFD SHIYSSYVVP PNYDSMIGKL ISYGATRAEA IARMRNALDE IIIDGIKTNI ELHQRILHDK SFMEGGTNIH YLEKMLKDS
NT Sequence
CTGTTTATGC TCAGTAAAAT AGTTATTGCT AATCGAGGCG AAATTGCGCT TCGTATACTG CGTGCTTGTA AAGAACTTGG CATTCAGACT GTTGCTGTTC ACTCTGATGT AGACAAAGAT TTATTGCACG TTCGCCTGGC TGATGAAACC GTATGCATAG GCCCTGCACC TGCCCAAAAA AGCTATCTCA ATATTCCTGC AATTATATCT GCGGCAGAAA TTACTGATGC AGTAGCGATA CATCCAGGTT ATGGATTTTT ATCAGAAAAT GCCGATTTTG CAGATATTGT TGAGCAAAGC GGTTTCCGTT TTATTGGCCC ACGCGGCGAC TCCATTCGTC TTATGGGGGA TAAAGTTTCC GCGATTGCTG CCATGAAAGC GGCAGGAGTA CCCTGTGTTC CCGGTTCAGA CGGTCCTTTA GGAGATGACG ACAACAAAAA TCTCGAGTTG GCGCGAAAAA TTGGTTATCC AATCATTATC AAAGCAGCCG GCGGCGGTGG TGGACGTGGC ATGAGAGTCG TTCATAGCGA ATCCAATTTA CTTAGTGCTA TTGCGTTAAC CCGAAGCGAA GCAAAAGCCG CTTTCAATAA CCCGATAGTT TACATGGAAA AATTTCTGGA AAATCCTCGC CATGTTGAAT TCCAAGTCCT TGGTGATGGG AAAGGGCATG CTATACATTT GGGTGAGCGT GATTGCTCGA TGCAAAGACG ACATCAAAAA GTCGTAGAAG AAGCTCCGGC TCCAGGAATC ACTCCTGAAT TAAGAGAAGA AATTGGCGCG TCAGTGATTA AAGCTTGCAA AGAGTTACAA TACCGAGGTG CTGGAACCTT TGAATTTTTA TTTCAAGATG GTTGTTTTTA TTTCATCGAA ATGAATACGC GCATTCAGGT TGAGCATCCA GTAACTGAAA TGATCACTGG GATTGATTTA ATTAAGGAAC AAATCAAAAT TGCCAGCGAT ATTCCTCTGA CATTAACCCA AAAAGATATT AAATTACGCG GCCATGCTAT TGAATGCCGA ATCAATGCGG AAGATCCAAG GAATTTTATG CCCTCTCCTG GAACCATAAA ATTACTTCAT CAACCAGGAG GCCCAGGCAT TCGTTTTGAT TCTCACATCT ACAGCAGCTA TGTTGTTCCA CCTAATTACG ATTCCATGAT AGGAAAATTA ATAAGTTATG GGGCAACAAG AGCAGAAGCG ATTGCCAGGA TGCGTAATGC CCTTGATGAA ATTATCATTG ATGGCATAAA GACAAATATT GAATTACACC AACGTATTCT TCATGACAAA TCTTTTATGG AAGGTGGCAC AAACATTCAC TATCTAGAAA AAATGCTCAA GGATTCA
Details for LepnA.00036.a.B1.GE41294
HARVESTED ON: 3/8/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMX XMLSKIVIAN RGEIALRILR ACKELGIQTV AVHSDVDKDL LHVRLADETV CIGPAPAQKS YLNIPAIISA AEITDAVAIH PGYGFLSENA DFADIVEQSG FRFIGPRGDS IRLMGDKVSA IAAMKAAGVP CVPGSDGPLG DDDNKNLELA RKIGYPIIIK AAGGGGGRGM RVVHSESNLL SAIALTRSEA KAAFNNPIVY MEKFLENPRH VEFQVLGDGK GHAIHLGERD CSMQRRHQKV VEEAPAPGIT PELREEIGAS VIKACKELQY RGAGTFEFLF QDGCFYFIEM NTRIQVEHPV TEMITGIDLI KEQIKIASDI PLTLTQKDIK LRGHAIECRI NAEDPRNFMP SPGTIKLLHQ PGGPGIRFDS HIYSSYVVPP NYDSMIGKLI SYGATRAEAI ARMRNALDEI IIDGIKTNIE LHQRILHDKS FMEGGTNIHY X
Validated NT Sequence
atggctcacc accaccacca ccatatgnnn nttatgctca gtaaaatagt tattgctaat cgaggcgaaa ttgcgcttcg tatactgcgt gcttgtaaag aacttggcat tcagactgtt gctgttcact ctgatgtaga caaagattta ttgcacgttc gcctggctga tgaaaccgta tgcataggcc ctgcacctgc ccaaaaaagc tatctcaata ttcctgcaat tatatctgcg gcagaaatta ctgatgcagt agcgatacat ccaggttatg gatttttatc agaaaatgcc gattttgcag atattgttga gcaaagcggt ttccgtttta ttggcccacg cggcgactcc attcgtctta tgggggataa agtttccgcg attgctgcca tgaaagcggc aggagtaccc tgtgttcccg gttcagacgg tcctttagga gatgacgaca acaaaaatct cgagttggcg cgaaaaattg gttatccaat cattatcaaa gcagccggcg gcggtggtgg acgtggcatg agagtcgttc atagcgaatc caatttactt agtgctattg cgttaacccg aagcgaagca aaagccgctt tcaataaccc gatagtttac atggaaaaat ttctggaaaa tcctcgccat gttgaattcc aagtccttgg tgatgggaaa gggcatgcta tacatttggg tgagcgtgat tgctcgatgc aaagacgaca tcaaaaagtc gtagaagaag ctccggctcc aggaatcact cctgaattaa gagaagaaat tggcgcgtca gtgattaaag cttgcaaaga gttacaatac cgaggtgctg gaacctttga atttttattt caagatggtt gtttttattt catcgaaatg aatacgcgca ttcaggttga gcatccagta actgaaatga tcactgggat tgatttaatt aaggaacaaa tcaaaattgc cagcgatatt cctctgacat taacccaaaa agatattaaa ttacgcggcc atgctattga atgccgaatc aatgcggaag atccaaggaa ttttatgccc tctcctggaa ccataaaatt acttcatcaa ccaggaggcc caggcattcg ttttgattct cacatctaca gcagctatgt tgttccacct aattacgatt ccatgatagg aaaattaata agttatgggg caacaagagc agaagcgatt gccaggatgc gtaatgccct tgatgaaatt atcattgatg gcataaagac aaatattgaa ttacaccaac gtattcttca tgacaaatct tttatggaag gtggcacaaa cattcactat nnag
Expected Protein Sequence
MAHHHHHHMF MLSKIVIANR GEIALRILRA CKELGIQTVA VHSDVDKDLL HVRLADETVC IGPAPAQKSY LNIPAIISAA EITDAVAIHP GYGFLSENAD FADIVEQSGF RFIGPRGDSI RLMGDKVSAI AAMKAAGVPC VPGSDGPLGD DDNKNLELAR KIGYPIIIKA AGGGGGRGMR VVHSESNLLS AIALTRSEAK AAFNNPIVYM EKFLENPRHV EFQVLGDGKG HAIHLGERDC SMQRRHQKVV EEAPAPGITP ELREEIGASV IKACKELQYR GAGTFEFLFQ DGCFYFIEMN TRIQVEHPVT EMITGIDLIK EQIKIASDIP LTLTQKDIKL RGHAIECRIN AEDPRNFMPS PGTIKLLHQP GGPGIRFDSH IYSSYVVPPN YDSMIGKLIS YGATRAEAIA RMRNALDEII IDGIKTNIEL HQRILHDKSF MEGGTNIHYL EKMLKDS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgttta tgctcagtaa aatagttatt gctaatcgag gcgaaattgc gcttcgtata ctgcgtgctt gtaaagaact tggcattcag actgttgctg ttcactctga tgtagacaaa gatttattgc acgttcgcct ggctgatgaa accgtatgca taggccctgc acctgcccaa aaaagctatc tcaatattcc tgcaattata tctgcggcag aaattactga tgcagtagcg atacatccag gttatggatt tttatcagaa aatgccgatt ttgcagatat tgttgagcaa agcggtttcc gttttattgg cccacgcggc gactccattc gtcttatggg ggataaagtt tccgcgattg ctgccatgaa agcggcagga gtaccctgtg ttcccggttc agacggtcct ttaggagatg acgacaacaa aaatctcgag ttggcgcgaa aaattggtta tccaatcatt atcaaagcag ccggcggcgg tggtggacgt ggcatgagag tcgttcatag cgaatccaat ttacttagtg ctattgcgtt aacccgaagc gaagcaaaag ccgctttcaa taacccgata gtttacatgg aaaaatttct ggaaaatcct cgccatgttg aattccaagt ccttggtgat gggaaagggc atgctataca tttgggtgag cgtgattgct cgatgcaaag acgacatcaa aaagtcgtag aagaagctcc ggctccagga atcactcctg aattaagaga agaaattggc gcgtcagtga ttaaagcttg caaagagtta caataccgag gtgctggaac ctttgaattt ttatttcaag atggttgttt ttatttcatc gaaatgaata cgcgcattca ggttgagcat ccagtaactg aaatgatcac tgggattgat ttaattaagg aacaaatcaa aattgccagc gatattcctc tgacattaac ccaaaaagat attaaattac gcggccatgc tattgaatgc cgaatcaatg cggaagatcc aaggaatttt atgccctctc ctggaaccat aaaattactt catcaaccag gaggcccagg cattcgtttt gattctcaca tctacagcag ctatgttgtt ccacctaatt acgattccat gataggaaaa ttaataagtt atggggcaac aagagcagaa gcgattgcca ggatgcgtaa tgcccttgat gaaattatca ttgatggcat aaagacaaat attgaattac accaacgtat tcttcatgac aaatctttta tggaaggtgg cacaaacatt cactatctag aaaaaatgct caaggattca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for LepnA.00036.a.B1.PW38292
PURIFICATION DATe: 5/31/2017
CONCENTRATION: 30.5mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX XMLSKIVIAN RGEIALRILR ACKELGIQTV AVHSDVDKDL LHVRLADETV CIGPAPAQKS YLNIPAIISA AEITDAVAIH PGYGFLSENA DFADIVEQSG FRFIGPRGDS IRLMGDKVSA IAAMKAAGVP CVPGSDGPLG DDDNKNLELA RKIGYPIIIK AAGGGGGRGM RVVHSESNLL SAIALTRSEA KAAFNNPIVY MEKFLENPRH VEFQVLGDGK GHAIHLGERD CSMQRRHQKV VEEAPAPGIT PELREEIGAS VIKACKELQY RGAGTFEFLF QDGCFYFIEM NTRIQVEHPV TEMITGIDLI KEQIKIASDI PLTLTQKDIK LRGHAIECRI NAEDPRNFMP SPGTIKLLHQ PGGPGIRFDS HIYSSYVVPP NYDSMIGKLI SYGATRAEAI ARMRNALDEI IIDGIKTNIE LHQRILHDKS FMEGGTNIHY X
Validated NT Sequence
atggctcacc accaccacca ccatatgnnn nttatgctca gtaaaatagt tattgctaat cgaggcgaaa ttgcgcttcg tatactgcgt gcttgtaaag aacttggcat tcagactgtt gctgttcact ctgatgtaga caaagattta ttgcacgttc gcctggctga tgaaaccgta tgcataggcc ctgcacctgc ccaaaaaagc tatctcaata ttcctgcaat tatatctgcg gcagaaatta ctgatgcagt agcgatacat ccaggttatg gatttttatc agaaaatgcc gattttgcag atattgttga gcaaagcggt ttccgtttta ttggcccacg cggcgactcc attcgtctta tgggggataa agtttccgcg attgctgcca tgaaagcggc aggagtaccc tgtgttcccg gttcagacgg tcctttagga gatgacgaca acaaaaatct cgagttggcg cgaaaaattg gttatccaat cattatcaaa gcagccggcg gcggtggtgg acgtggcatg agagtcgttc atagcgaatc caatttactt agtgctattg cgttaacccg aagcgaagca aaagccgctt tcaataaccc gatagtttac atggaaaaat ttctggaaaa tcctcgccat gttgaattcc aagtccttgg tgatgggaaa gggcatgcta tacatttggg tgagcgtgat tgctcgatgc aaagacgaca tcaaaaagtc gtagaagaag ctccggctcc aggaatcact cctgaattaa gagaagaaat tggcgcgtca gtgattaaag cttgcaaaga gttacaatac cgaggtgctg gaacctttga atttttattt caagatggtt gtttttattt catcgaaatg aatacgcgca ttcaggttga gcatccagta actgaaatga tcactgggat tgatttaatt aaggaacaaa tcaaaattgc cagcgatatt cctctgacat taacccaaaa agatattaaa ttacgcggcc atgctattga atgccgaatc aatgcggaag atccaaggaa ttttatgccc tctcctggaa ccataaaatt acttcatcaa ccaggaggcc caggcattcg ttttgattct cacatctaca gcagctatgt tgttccacct aattacgatt ccatgatagg aaaattaata agttatgggg caacaagagc agaagcgatt gccaggatgc gtaatgccct tgatgaaatt atcattgatg gcataaagac aaatattgaa ttacaccaac gtattcttca tgacaaatct tttatggaag gtggcacaaa cattcactat nnag
Expressed Protein Sequence
MAHHHHHHMF MLSKIVIANR GEIALRILRA CKELGIQTVA VHSDVDKDLL HVRLADETVC IGPAPAQKSY LNIPAIISAA EITDAVAIHP GYGFLSENAD FADIVEQSGF RFIGPRGDSI RLMGDKVSAI AAMKAAGVPC VPGSDGPLGD DDNKNLELAR KIGYPIIIKA AGGGGGRGMR VVHSESNLLS AIALTRSEAK AAFNNPIVYM EKFLENPRHV EFQVLGDGKG HAIHLGERDC SMQRRHQKVV EEAPAPGITP ELREEIGASV IKACKELQYR GAGTFEFLFQ DGCFYFIEMN TRIQVEHPVT EMITGIDLIK EQIKIASDIP LTLTQKDIKL RGHAIECRIN AEDPRNFMPS PGTIKLLHQP GGPGIRFDSH IYSSYVVPPN YDSMIGKLIS YGATRAEAIA RMRNALDEII IDGIKTNIEL HQRILHDKSF MEGGTNIHYL EKMLKDS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGTTTA TGCTCAGTAA AATAGTTATT GCTAATCGAG GCGAAATTGC GCTTCGTATA CTGCGTGCTT GTAAAGAACT TGGCATTCAG ACTGTTGCTG TTCACTCTGA TGTAGACAAA GATTTATTGC ACGTTCGCCT GGCTGATGAA ACCGTATGCA TAGGCCCTGC ACCTGCCCAA AAAAGCTATC TCAATATTCC TGCAATTATA TCTGCGGCAG AAATTACTGA TGCAGTAGCG ATACATCCAG GTTATGGATT TTTATCAGAA AATGCCGATT TTGCAGATAT TGTTGAGCAA AGCGGTTTCC GTTTTATTGG CCCACGCGGC GACTCCATTC GTCTTATGGG GGATAAAGTT TCCGCGATTG CTGCCATGAA AGCGGCAGGA GTACCCTGTG TTCCCGGTTC AGACGGTCCT TTAGGAGATG ACGACAACAA AAATCTCGAG TTGGCGCGAA AAATTGGTTA TCCAATCATT ATCAAAGCAG CCGGCGGCGG TGGTGGACGT GGCATGAGAG TCGTTCATAG CGAATCCAAT TTACTTAGTG CTATTGCGTT AACCCGAAGC GAAGCAAAAG CCGCTTTCAA TAACCCGATA GTTTACATGG AAAAATTTCT GGAAAATCCT CGCCATGTTG AATTCCAAGT CCTTGGTGAT GGGAAAGGGC ATGCTATACA TTTGGGTGAG CGTGATTGCT CGATGCAAAG ACGACATCAA AAAGTCGTAG AAGAAGCTCC GGCTCCAGGA ATCACTCCTG AATTAAGAGA AGAAATTGGC GCGTCAGTGA TTAAAGCTTG CAAAGAGTTA CAATACCGAG GTGCTGGAAC CTTTGAATTT TTATTTCAAG ATGGTTGTTT TTATTTCATC GAAATGAATA CGCGCATTCA GGTTGAGCAT CCAGTAACTG AAATGATCAC TGGGATTGAT TTAATTAAGG AACAAATCAA AATTGCCAGC GATATTCCTC TGACATTAAC CCAAAAAGAT ATTAAATTAC GCGGCCATGC TATTGAATGC CGAATCAATG CGGAAGATCC AAGGAATTTT ATGCCCTCTC CTGGAACCAT AAAATTACTT CATCAACCAG GAGGCCCAGG CATTCGTTTT GATTCTCACA TCTACAGCAG CTATGTTGTT CCACCTAATT ACGATTCCAT GATAGGAAAA TTAATAAGTT ATGGGGCAAC AAGAGCAGAA GCGATTGCCA GGATGCGTAA TGCCCTTGAT GAAATTATCA TTGATGGCAT AAAGACAAAT ATTGAATTAC ACCAACGTAT TCTTCATGAC AAATCTTTTA TGGAAGGTGG CACAAACATT CACTATCTAG AAAAAATGCT CAAGGATTCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for LepnA.00036.a.B1.PW38293
PURIFICATION DATe: 5/31/2017
CONCENTRATION: 13.2mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX XMLSKIVIAN RGEIALRILR ACKELGIQTV AVHSDVDKDL LHVRLADETV CIGPAPAQKS YLNIPAIISA AEITDAVAIH PGYGFLSENA DFADIVEQSG FRFIGPRGDS IRLMGDKVSA IAAMKAAGVP CVPGSDGPLG DDDNKNLELA RKIGYPIIIK AAGGGGGRGM RVVHSESNLL SAIALTRSEA KAAFNNPIVY MEKFLENPRH VEFQVLGDGK GHAIHLGERD CSMQRRHQKV VEEAPAPGIT PELREEIGAS VIKACKELQY RGAGTFEFLF QDGCFYFIEM NTRIQVEHPV TEMITGIDLI KEQIKIASDI PLTLTQKDIK LRGHAIECRI NAEDPRNFMP SPGTIKLLHQ PGGPGIRFDS HIYSSYVVPP NYDSMIGKLI SYGATRAEAI ARMRNALDEI IIDGIKTNIE LHQRILHDKS FMEGGTNIHY X
Validated NT Sequence
atggctcacc accaccacca ccatatgnnn nttatgctca gtaaaatagt tattgctaat cgaggcgaaa ttgcgcttcg tatactgcgt gcttgtaaag aacttggcat tcagactgtt gctgttcact ctgatgtaga caaagattta ttgcacgttc gcctggctga tgaaaccgta tgcataggcc ctgcacctgc ccaaaaaagc tatctcaata ttcctgcaat tatatctgcg gcagaaatta ctgatgcagt agcgatacat ccaggttatg gatttttatc agaaaatgcc gattttgcag atattgttga gcaaagcggt ttccgtttta ttggcccacg cggcgactcc attcgtctta tgggggataa agtttccgcg attgctgcca tgaaagcggc aggagtaccc tgtgttcccg gttcagacgg tcctttagga gatgacgaca acaaaaatct cgagttggcg cgaaaaattg gttatccaat cattatcaaa gcagccggcg gcggtggtgg acgtggcatg agagtcgttc atagcgaatc caatttactt agtgctattg cgttaacccg aagcgaagca aaagccgctt tcaataaccc gatagtttac atggaaaaat ttctggaaaa tcctcgccat gttgaattcc aagtccttgg tgatgggaaa gggcatgcta tacatttggg tgagcgtgat tgctcgatgc aaagacgaca tcaaaaagtc gtagaagaag ctccggctcc aggaatcact cctgaattaa gagaagaaat tggcgcgtca gtgattaaag cttgcaaaga gttacaatac cgaggtgctg gaacctttga atttttattt caagatggtt gtttttattt catcgaaatg aatacgcgca ttcaggttga gcatccagta actgaaatga tcactgggat tgatttaatt aaggaacaaa tcaaaattgc cagcgatatt cctctgacat taacccaaaa agatattaaa ttacgcggcc atgctattga atgccgaatc aatgcggaag atccaaggaa ttttatgccc tctcctggaa ccataaaatt acttcatcaa ccaggaggcc caggcattcg ttttgattct cacatctaca gcagctatgt tgttccacct aattacgatt ccatgatagg aaaattaata agttatgggg caacaagagc agaagcgatt gccaggatgc gtaatgccct tgatgaaatt atcattgatg gcataaagac aaatattgaa ttacaccaac gtattcttca tgacaaatct tttatggaag gtggcacaaa cattcactat nnag
Expressed Protein Sequence
MAHHHHHHMF MLSKIVIANR GEIALRILRA CKELGIQTVA VHSDVDKDLL HVRLADETVC IGPAPAQKSY LNIPAIISAA EITDAVAIHP GYGFLSENAD FADIVEQSGF RFIGPRGDSI RLMGDKVSAI AAMKAAGVPC VPGSDGPLGD DDNKNLELAR KIGYPIIIKA AGGGGGRGMR VVHSESNLLS AIALTRSEAK AAFNNPIVYM EKFLENPRHV EFQVLGDGKG HAIHLGERDC SMQRRHQKVV EEAPAPGITP ELREEIGASV IKACKELQYR GAGTFEFLFQ DGCFYFIEMN TRIQVEHPVT EMITGIDLIK EQIKIASDIP LTLTQKDIKL RGHAIECRIN AEDPRNFMPS PGTIKLLHQP GGPGIRFDSH IYSSYVVPPN YDSMIGKLIS YGATRAEAIA RMRNALDEII IDGIKTNIEL HQRILHDKSF MEGGTNIHYL EKMLKDS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCTGTTTA TGCTCAGTAA AATAGTTATT GCTAATCGAG GCGAAATTGC GCTTCGTATA CTGCGTGCTT GTAAAGAACT TGGCATTCAG ACTGTTGCTG TTCACTCTGA TGTAGACAAA GATTTATTGC ACGTTCGCCT GGCTGATGAA ACCGTATGCA TAGGCCCTGC ACCTGCCCAA AAAAGCTATC TCAATATTCC TGCAATTATA TCTGCGGCAG AAATTACTGA TGCAGTAGCG ATACATCCAG GTTATGGATT TTTATCAGAA AATGCCGATT TTGCAGATAT TGTTGAGCAA AGCGGTTTCC GTTTTATTGG CCCACGCGGC GACTCCATTC GTCTTATGGG GGATAAAGTT TCCGCGATTG CTGCCATGAA AGCGGCAGGA GTACCCTGTG TTCCCGGTTC AGACGGTCCT TTAGGAGATG ACGACAACAA AAATCTCGAG TTGGCGCGAA AAATTGGTTA TCCAATCATT ATCAAAGCAG CCGGCGGCGG TGGTGGACGT GGCATGAGAG TCGTTCATAG CGAATCCAAT TTACTTAGTG CTATTGCGTT AACCCGAAGC GAAGCAAAAG CCGCTTTCAA TAACCCGATA GTTTACATGG AAAAATTTCT GGAAAATCCT CGCCATGTTG AATTCCAAGT CCTTGGTGAT GGGAAAGGGC ATGCTATACA TTTGGGTGAG CGTGATTGCT CGATGCAAAG ACGACATCAA AAAGTCGTAG AAGAAGCTCC GGCTCCAGGA ATCACTCCTG AATTAAGAGA AGAAATTGGC GCGTCAGTGA TTAAAGCTTG CAAAGAGTTA CAATACCGAG GTGCTGGAAC CTTTGAATTT TTATTTCAAG ATGGTTGTTT TTATTTCATC GAAATGAATA CGCGCATTCA GGTTGAGCAT CCAGTAACTG AAATGATCAC TGGGATTGAT TTAATTAAGG AACAAATCAA AATTGCCAGC GATATTCCTC TGACATTAAC CCAAAAAGAT ATTAAATTAC GCGGCCATGC TATTGAATGC CGAATCAATG CGGAAGATCC AAGGAATTTT ATGCCCTCTC CTGGAACCAT AAAATTACTT CATCAACCAG GAGGCCCAGG CATTCGTTTT GATTCTCACA TCTACAGCAG CTATGTTGTT CCACCTAATT ACGATTCCAT GATAGGAAAA TTAATAAGTT ATGGGGCAAC AAGAGCAGAA GCGATTGCCA GGATGCGTAA TGCCCTTGAT GAAATTATCA TTGATGGCAT AAAGACAAAT ATTGAATTAC ACCAACGTAT TCTTCATGAC AAATCTTTTA TGGAAGGTGG CACAAACATT CACTATCTAG AAAAAATGCT CAAGGATTCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT