LepnA.00027.b

Acyl CoA dehydrogenase, short chain specific

CENTER ID: LepnA.00027.b
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00027.b.B1.GE41292 Full length(LepnA.00027.b) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00027.b.B1.PW38291 Full length(LepnA.00027.b) 1 384
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|272624.6.peg.900
UniProt: Q5ZX64
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHFNFTDEHL AFREMASEFA RNKLAPMADF WDEQGYFPIE TLREAAQLGM AGMVVREDIG GAQLSRLDAA LIFEQLATGC IPTSAYLSIH NMVASLVDRY GSQEIRKKWG PKLTSMEVIA SYCLTEPESG SDAASLKTRA VKEGEYYVLN GAKAFISGGS VSDVYLCMVR TGDESHHGIS CLLIEKNTPG LTFGKLEKKL GWRNQPTCML YFENCRVPIA NRVGEEGMGF KIALNALNGG RVNIASCSLG GALACLRMSQ SYMHERKQFG KPLTQMQALR FYFADMLTDY EAARLMVYRA AAAMDNDDPN APMYCAMGKR LATDVAFRIS DKAMQLHGGY GYLRDYQIER IFRDLRVHQI LEGTNEIMRE IIAKASLDEE YFIE
NT Sequence
ATGCATTTTA ATTTCACTGA TGAACATTTG GCATTTCGAG AAATGGCCTC CGAGTTTGCT CGTAACAAAC TGGCTCCAAT GGCAGATTTC TGGGATGAAC AAGGTTACTT TCCAATCGAA ACTTTAAGAG AAGCGGCACA ATTAGGTATG GCGGGAATGG TAGTCCGCGA AGACATAGGA GGTGCTCAGC TCTCTCGTCT GGATGCAGCA CTCATCTTTG AACAACTCGC TACGGGCTGT ATTCCAACAA GTGCATATCT CTCCATCCAT AATATGGTTG CTTCATTGGT CGACCGCTAT GGCAGTCAAG AGATTCGCAA GAAATGGGGG CCAAAATTAA CCAGTATGGA AGTGATAGCC AGTTATTGTC TTACAGAGCC AGAATCCGGA TCTGATGCAG CCTCTTTAAA AACTCGCGCG GTAAAAGAGG GTGAATATTA TGTTCTTAAT GGTGCGAAAG CATTCATATC GGGTGGAAGT GTTAGTGATG TGTATTTATG TATGGTACGT ACAGGCGATG AATCTCATCA TGGCATCAGT TGTTTGTTAA TTGAAAAGAA TACTCCTGGT CTGACATTTG GCAAACTGGA GAAAAAATTA GGTTGGCGAA ATCAGCCAAC TTGTATGCTA TATTTTGAAA ATTGTCGTGT GCCCATTGCC AATCGTGTTG GTGAAGAAGG TATGGGTTTT AAAATCGCAC TGAATGCCTT AAACGGAGGG AGAGTGAATA TTGCTTCCTG CTCATTGGGA GGGGCTCTTG CTTGCCTGCG AATGAGCCAA TCTTATATGC ATGAGCGTAA ACAGTTTGGA AAACCTTTAA CTCAGATGCA AGCCCTTCGT TTTTATTTTG CGGATATGTT AACAGATTAT GAAGCGGCAA GGCTGATGGT TTATAGGGCT GCAGCAGCAA TGGATAATGA TGATCCCAAT GCGCCAATGT ATTGTGCTAT GGGCAAGCGC TTGGCTACAG ATGTCGCATT TCGTATTAGT GATAAAGCCA TGCAATTGCA TGGAGGTTAT GGCTACCTGC GAGATTATCA AATTGAAAGA ATATTTAGAG ATTTAAGAGT TCATCAAATT CTTGAAGGCA CTAATGAAAT CATGCGTGAA ATTATAGCGA AAGCAAGTTT GGATGAAGAG TATTTTATAG AG
Details for LepnA.00027.b.B1.GE41292
HARVESTED ON: 3/10/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMH FNFTDEHLAF REMASEFARN KLAPMADFWD EQGYFPIETL REAAQLGMAG MVVREDIGGA QLSRLDAALI FEQLATGCIP TSAYLSIHNM VASLVDRYGS QEIRKKWGPK LTSMEVIASY CLTEPESGSD AASLKTRAVK EGEYYVLNGA KAFISGGSVS DVYLCMVRTG DESHHGISCL LIEKNTPGLT FGKLEKKLGW RNQPTCMLYF ENCRVPIANR VGEEGMGFKI ALNALNGGRV NIASCSLGGA LACLRMSQSY MHERKQFGKP LTQMQALRFY FADMLTDYEA ARLMVYRAAA AMDNDDPNAP MYCAMGKRLA TDVAFRISDK AMQLHGGYGY LRDYQIERIF RDLRVHQILE GTNEIMREII AKA
Validated NT Sequence
atggctcacc accaccacca ccatatgcat tttaatttca ctgatgaaca tttggcattt cgagaaatgg cctccgagtt tgctcgtaac aaactggctc caatggcaga tttctgggat gaacaaggtt actttccaat cgaaacttta agagaagcgg cacaattagg tatggcggga atggtagtcc gcgaagacat aggaggtgct cagctctctc gtctggatgc agcactcatc tttgaacaac tcgctacggg ctgtattcca acaagtgcat atctctccat ccataatatg gttgcttcat tggtcgaccg ctatggcagt caagagattc gcaagaaatg ggggccaaaa ttaaccagta tggaagtgat agccagttat tgtcttacag agccagaatc cggatctgat gcagcctctt taaaaactcg cgcggtaaaa gagggtgaat attatgttct taatggtgcg aaagcattca tatcgggtgg aagtgttagt gatgtgtatt tatgtatggt acgtacaggc gatgaatctc atcatggcat cagttgtttg ttaattgaaa agaatactcc tggtctgaca tttggcaaac tggagaaaaa attaggttgg cgaaatcagc caacttgtat gctatatttt gaaaattgtc gtgtgcccat tgccaatcgt gttggtgaag aaggtatggg ttttaaaatc gcactgaatg ccttaaacgg agggagagtg aatattgctt cctgctcatt gggaggggct cttgcttgcc tgcgaatgag ccaatcttat atgcatgagc gtaaacagtt tggaaaacct ttaactcaga tgcaagccct tcgtttttat tttgcggata tgttaacaga ttatgaagcg gcaaggctga tggtttatag ggctgcagca gcaatggata atgatgatcc caatgcgcca atgtattgtg ctatgggcaa gcgcttggct acagatgtcg catttcgtat tagtgataaa gccatgcaat tgcatggagg ttatggctac ctgcgagatt atcaaattga aagaatattt agagatttaa gagttcatca aattcttgaa ggcactaatg aaatcatgcg tgaaattata gcgaaagcaa gt
Expected Protein Sequence
MAHHHHHHMH FNFTDEHLAF REMASEFARN KLAPMADFWD EQGYFPIETL REAAQLGMAG MVVREDIGGA QLSRLDAALI FEQLATGCIP TSAYLSIHNM VASLVDRYGS QEIRKKWGPK LTSMEVIASY CLTEPESGSD AASLKTRAVK EGEYYVLNGA KAFISGGSVS DVYLCMVRTG DESHHGISCL LIEKNTPGLT FGKLEKKLGW RNQPTCMLYF ENCRVPIANR VGEEGMGFKI ALNALNGGRV NIASCSLGGA LACLRMSQSY MHERKQFGKP LTQMQALRFY FADMLTDYEA ARLMVYRAAA AMDNDDPNAP MYCAMGKRLA TDVAFRISDK AMQLHGGYGY LRDYQIERIF RDLRVHQILE GTNEIMREII AKASLDEEYF IE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatt ttaatttcac tgatgaacat ttggcatttc gagaaatggc ctccgagttt gctcgtaaca aactggctcc aatggcagat ttctgggatg aacaaggtta ctttccaatc gaaactttaa gagaagcggc acaattaggt atggcgggaa tggtagtccg cgaagacata ggaggtgctc agctctctcg tctggatgca gcactcatct ttgaacaact cgctacgggc tgtattccaa caagtgcata tctctccatc cataatatgg ttgcttcatt ggtcgaccgc tatggcagtc aagagattcg caagaaatgg gggccaaaat taaccagtat ggaagtgata gccagttatt gtcttacaga gccagaatcc ggatctgatg cagcctcttt aaaaactcgc gcggtaaaag agggtgaata ttatgttctt aatggtgcga aagcattcat atcgggtgga agtgttagtg atgtgtattt atgtatggta cgtacaggcg atgaatctca tcatggcatc agttgtttgt taattgaaaa gaatactcct ggtctgacat ttggcaaact ggagaaaaaa ttaggttggc gaaatcagcc aacttgtatg ctatattttg aaaattgtcg tgtgcccatt gccaatcgtg ttggtgaaga aggtatgggt tttaaaatcg cactgaatgc cttaaacgga gggagagtga atattgcttc ctgctcattg ggaggggctc ttgcttgcct gcgaatgagc caatcttata tgcatgagcg taaacagttt ggaaaacctt taactcagat gcaagccctt cgtttttatt ttgcggatat gttaacagat tatgaagcgg caaggctgat ggtttatagg gctgcagcag caatggataa tgatgatccc aatgcgccaa tgtattgtgc tatgggcaag cgcttggcta cagatgtcgc atttcgtatt agtgataaag ccatgcaatt gcatggaggt tatggctacc tgcgagatta tcaaattgaa agaatattta gagatttaag agttcatcaa attcttgaag gcactaatga aatcatgcgt gaaattatag cgaaagcaag tttggatgaa gagtatttta tagagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LepnA.00027.b.B1.PW38291
PURIFICATION DATe: 5/31/2017
CONCENTRATION: 34.5mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMH FNFTDEHLAF REMASEFARN KLAPMADFWD EQGYFPIETL REAAQLGMAG MVVREDIGGA QLSRLDAALI FEQLATGCIP TSAYLSIHNM VASLVDRYGS QEIRKKWGPK LTSMEVIASY CLTEPESGSD AASLKTRAVK EGEYYVLNGA KAFISGGSVS DVYLCMVRTG DESHHGISCL LIEKNTPGLT FGKLEKKLGW RNQPTCMLYF ENCRVPIANR VGEEGMGFKI ALNALNGGRV NIASCSLGGA LACLRMSQSY MHERKQFGKP LTQMQALRFY FADMLTDYEA ARLMVYRAAA AMDNDDPNAP MYCAMGKRLA TDVAFRISDK AMQLHGGYGY LRDYQIERIF RDLRVHQILE GTNEIMREII AKA
Validated NT Sequence
atggctcacc accaccacca ccatatgcat tttaatttca ctgatgaaca tttggcattt cgagaaatgg cctccgagtt tgctcgtaac aaactggctc caatggcaga tttctgggat gaacaaggtt actttccaat cgaaacttta agagaagcgg cacaattagg tatggcggga atggtagtcc gcgaagacat aggaggtgct cagctctctc gtctggatgc agcactcatc tttgaacaac tcgctacggg ctgtattcca acaagtgcat atctctccat ccataatatg gttgcttcat tggtcgaccg ctatggcagt caagagattc gcaagaaatg ggggccaaaa ttaaccagta tggaagtgat agccagttat tgtcttacag agccagaatc cggatctgat gcagcctctt taaaaactcg cgcggtaaaa gagggtgaat attatgttct taatggtgcg aaagcattca tatcgggtgg aagtgttagt gatgtgtatt tatgtatggt acgtacaggc gatgaatctc atcatggcat cagttgtttg ttaattgaaa agaatactcc tggtctgaca tttggcaaac tggagaaaaa attaggttgg cgaaatcagc caacttgtat gctatatttt gaaaattgtc gtgtgcccat tgccaatcgt gttggtgaag aaggtatggg ttttaaaatc gcactgaatg ccttaaacgg agggagagtg aatattgctt cctgctcatt gggaggggct cttgcttgcc tgcgaatgag ccaatcttat atgcatgagc gtaaacagtt tggaaaacct ttaactcaga tgcaagccct tcgtttttat tttgcggata tgttaacaga ttatgaagcg gcaaggctga tggtttatag ggctgcagca gcaatggata atgatgatcc caatgcgcca atgtattgtg ctatgggcaa gcgcttggct acagatgtcg catttcgtat tagtgataaa gccatgcaat tgcatggagg ttatggctac ctgcgagatt atcaaattga aagaatattt agagatttaa gagttcatca aattcttgaa ggcactaatg aaatcatgcg tgaaattata gcgaaagcaa gt
Expressed Protein Sequence
MAHHHHHHMH FNFTDEHLAF REMASEFARN KLAPMADFWD EQGYFPIETL REAAQLGMAG MVVREDIGGA QLSRLDAALI FEQLATGCIP TSAYLSIHNM VASLVDRYGS QEIRKKWGPK LTSMEVIASY CLTEPESGSD AASLKTRAVK EGEYYVLNGA KAFISGGSVS DVYLCMVRTG DESHHGISCL LIEKNTPGLT FGKLEKKLGW RNQPTCMLYF ENCRVPIANR VGEEGMGFKI ALNALNGGRV NIASCSLGGA LACLRMSQSY MHERKQFGKP LTQMQALRFY FADMLTDYEA ARLMVYRAAA AMDNDDPNAP MYCAMGKRLA TDVAFRISDK AMQLHGGYGY LRDYQIERIF RDLRVHQILE GTNEIMREII AKASLDEEYF IE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATT TTAATTTCAC TGATGAACAT TTGGCATTTC GAGAAATGGC CTCCGAGTTT GCTCGTAACA AACTGGCTCC AATGGCAGAT TTCTGGGATG AACAAGGTTA CTTTCCAATC GAAACTTTAA GAGAAGCGGC ACAATTAGGT ATGGCGGGAA TGGTAGTCCG CGAAGACATA GGAGGTGCTC AGCTCTCTCG TCTGGATGCA GCACTCATCT TTGAACAACT CGCTACGGGC TGTATTCCAA CAAGTGCATA TCTCTCCATC CATAATATGG TTGCTTCATT GGTCGACCGC TATGGCAGTC AAGAGATTCG CAAGAAATGG GGGCCAAAAT TAACCAGTAT GGAAGTGATA GCCAGTTATT GTCTTACAGA GCCAGAATCC GGATCTGATG CAGCCTCTTT AAAAACTCGC GCGGTAAAAG AGGGTGAATA TTATGTTCTT AATGGTGCGA AAGCATTCAT ATCGGGTGGA AGTGTTAGTG ATGTGTATTT ATGTATGGTA CGTACAGGCG ATGAATCTCA TCATGGCATC AGTTGTTTGT TAATTGAAAA GAATACTCCT GGTCTGACAT TTGGCAAACT GGAGAAAAAA TTAGGTTGGC GAAATCAGCC AACTTGTATG CTATATTTTG AAAATTGTCG TGTGCCCATT GCCAATCGTG TTGGTGAAGA AGGTATGGGT TTTAAAATCG CACTGAATGC CTTAAACGGA GGGAGAGTGA ATATTGCTTC CTGCTCATTG GGAGGGGCTC TTGCTTGCCT GCGAATGAGC CAATCTTATA TGCATGAGCG TAAACAGTTT GGAAAACCTT TAACTCAGAT GCAAGCCCTT CGTTTTTATT TTGCGGATAT GTTAACAGAT TATGAAGCGG CAAGGCTGAT GGTTTATAGG GCTGCAGCAG CAATGGATAA TGATGATCCC AATGCGCCAA TGTATTGTGC TATGGGCAAG CGCTTGGCTA CAGATGTCGC ATTTCGTATT AGTGATAAAG CCATGCAATT GCATGGAGGT TATGGCTACC TGCGAGATTA TCAAATTGAA AGAATATTTA GAGATTTAAG AGTTCATCAA ATTCTTGAAG GCACTAATGA AATCATGCGT GAAATTATAG CGAAAGCAAG TTTGGATGAA GAGTATTTTA TAGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT