LepnA.00027.a

Glutaryl CoA dehydrogenase

CENTER ID: LepnA.00027.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00027.a.B1.GE41291 Full length(LepnA.00027.a) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LepnA.00027.a.B1.PS38392 Full length(LepnA.00027.a) 1 385

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272624.6.peg.2750
UniProt: Q5ZSD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDLLFLDEQ LHDDERMIRD SVSRFVSNDV IPLMAESYEH AHFPKELIKK SADLGLLGLT LPAEYGGAEA SYVAYGLVCQ ELERGDSGLR SFVSVQSSLC MYPIFKFGSE EQKKRYLPEM AAGNIIGCFG LTEPDSGSDP ASMRTHAKKI EGGWILNGAK MWITNAPIAD IAIVWAKTEA GIRGFIVDTK SEGMSCPEIK LKMSLRASIT GELVFDNVFV PDENLLPKSE KGLGAALSCL SQARYGIAWG AMGAAMACFD IARDYLLERK QFAKPLASFQ LIQKDLADMY TEIIKAQCLN LQIGRLKDQH RETPTMISLA KGNACREALK IARKCRNLMG GNGISLEYHV IRHMLNLESV FTYEGTDNVH TLVLGRHITG INAFG
NT Sequence
GTGGACGACT TGTTGTTTCT TGATGAACAA TTACATGATG ACGAGCGCAT GATACGAGAT AGTGTGTCTC GTTTTGTTAG CAATGACGTA ATACCTTTAA TGGCTGAATC TTATGAGCAC GCCCATTTCC CAAAAGAGCT GATAAAAAAA TCTGCTGATC TGGGGTTGTT GGGCTTAACT TTACCTGCGG AATATGGGGG TGCAGAAGCT TCTTATGTTG CCTATGGTTT AGTATGCCAG GAATTGGAGC GAGGTGATAG CGGTTTGCGC AGCTTTGTTT CTGTGCAAAG TTCTTTGTGT ATGTATCCTA TTTTTAAATT TGGCAGTGAA GAGCAAAAAA AACGCTACCT GCCAGAGATG GCTGCGGGGA ATATTATAGG TTGCTTTGGA TTAACTGAGC CAGACTCAGG ATCTGACCCG GCCAGCATGC GTACTCATGC CAAAAAAATT GAAGGTGGCT GGATTTTGAA TGGTGCTAAA ATGTGGATCA CCAATGCCCC TATCGCAGAC ATCGCAATAG TGTGGGCAAA AACAGAGGCG GGAATTCGTG GTTTTATTGT CGATACAAAA TCTGAAGGCA TGAGTTGTCC TGAAATTAAG TTAAAGATGT CTTTACGAGC CTCCATTACC GGGGAGCTGG TTTTTGATAA TGTTTTTGTT CCAGACGAGA ATTTATTGCC GAAAAGTGAA AAAGGGCTTG GAGCTGCGTT AAGTTGCTTG AGTCAGGCGC GTTATGGTAT CGCATGGGGA GCAATGGGCG CTGCCATGGC TTGCTTTGAT ATCGCTCGGG ATTATTTGCT GGAACGCAAG CAGTTTGCCA AACCTTTGGC TTCGTTTCAG TTGATTCAAA AAGATTTGGC TGATATGTAT ACAGAAATTA TTAAAGCACA ATGCCTTAAT TTGCAGATTG GGCGTTTAAA GGATCAGCAT CGTGAGACAC CGACTATGAT TTCTCTGGCT AAGGGGAATG CTTGCCGTGA AGCATTGAAA ATTGCGAGAA AATGTAGGAA TCTAATGGGC GGGAATGGAA TTAGTCTTGA GTATCATGTC ATTCGGCATA TGCTGAATCT GGAGTCGGTC TTCACTTATG AAGGTACCGA TAATGTGCAT ACTTTGGTCT TGGGAAGACA TATAACGGGA ATTAATGCTT TTGGT
Details for LepnA.00027.a.B1.GE41291
HARVESTED ON: 3/10/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHXX XDLLFLDEQL HDDERMIRDS VSRFVSNDVI PLMAESYEHA HFPKELIKKS ADLGLLGLTL PAEYGGAEAS YVAYGLVCQE LERGDSGLRS FVSVQSSLCM YPIFKFGSEE QKKRYLPEMA AGNIIGCFGL TEPDSGSDPA SMRTHAKKIE GGWILNGAKM WITNAPIADI AIVWAKTEAG IRGFIVDTKS EGMSCPEIKL KMSLRASITG ELVFDNVFVP DENLLPKSEK GLGAALSCLS QARYGIAWGA MGAAMACFDI ARDYLLERKQ FAKPLASFQL IQKDLADMYT EIIKAQCLNL QIGRLKDQHR ETPTMISLAK GNACREALKI ARKCRNLMGG NGISLEYHVI RHMLNLESVF TYEGTDNVHT LVLGRH
Validated NT Sequence
atggctcacc accaccacca ccatatnnnn nnngacttgt tgtttcttga tgaacaatta catgatgacg agcgcatgat acgagatagt gtgtctcgtt ttgttagcaa tgacgtaata cctttaatgg ctgaatctta tgagcacgcc catttcccaa aagagctgat aaaaaaatct gctgatctgg ggttgttggg cttaacttta cctgcggaat atgggggtgc agaagcttct tatgttgcct atggtttagt atgccaggaa ttggagcgag gtgatagcgg tttgcgcagc tttgtttctg tgcaaagttc tttgtgtatg tatcctattt ttaaatttgg cagtgaagag caaaaaaaac gctacctgcc agagatggct gcggggaata ttataggttg ctttggatta actgagccag actcaggatc tgacccggcc agcatgcgta ctcatgccaa aaaaattgaa ggtggctgga ttttgaatgg tgctaaaatg tggatcacca atgcccctat cgcagacatc gcaatagtgt gggcaaaaac agaggcggga attcgtggtt ttattgtcga tacaaaatct gaaggcatga gttgtcctga aattaagtta aagatgtctt tacgagcctc cattaccggg gagctggttt ttgataatgt ttttgttcca gacgagaatt tattgccgaa aagtgaaaaa gggcttggag ctgcgttaag ttgcttgagt caggcgcgtt atggtatcgc atggggagca atgggcgctg ccatggcttg ctttgatatc gctcgggatt atttgctgga acgcaagcag tttgccaaac ctttggcttc gtttcagttg attcaaaaag atttggctga tatgtataca gaaattatta aagcacaatg ccttaatttg cagattgggc gtttaaagga tcagcatcgt gagacaccga ctatgatttc tctggctaag gggaatgctt gccgtgaagc attgaaaatt gcgagaaaat gtaggaatct aatgggcggg aatggaatta gtcttgagta tcatgtcatt cggcatatgc tgaatctgga gtcggtcttc acttatgaag gtaccgataa tgtgcatact ttggtcttgg gaagacatat a
Expected Protein Sequence
MAHHHHHHMD DLLFLDEQLH DDERMIRDSV SRFVSNDVIP LMAESYEHAH FPKELIKKSA DLGLLGLTLP AEYGGAEASY VAYGLVCQEL ERGDSGLRSF VSVQSSLCMY PIFKFGSEEQ KKRYLPEMAA GNIIGCFGLT EPDSGSDPAS MRTHAKKIEG GWILNGAKMW ITNAPIADIA IVWAKTEAGI RGFIVDTKSE GMSCPEIKLK MSLRASITGE LVFDNVFVPD ENLLPKSEKG LGAALSCLSQ ARYGIAWGAM GAAMACFDIA RDYLLERKQF AKPLASFQLI QKDLADMYTE IIKAQCLNLQ IGRLKDQHRE TPTMISLAKG NACREALKIA RKCRNLMGGN GISLEYHVIR HMLNLESVFT YEGTDNVHTL VLGRHITGIN AFG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggacg acttgttgtt tcttgatgaa caattacatg atgacgagcg catgatacga gatagtgtgt ctcgttttgt tagcaatgac gtaatacctt taatggctga atcttatgag cacgcccatt tcccaaaaga gctgataaaa aaatctgctg atctggggtt gttgggctta actttacctg cggaatatgg gggtgcagaa gcttcttatg ttgcctatgg tttagtatgc caggaattgg agcgaggtga tagcggtttg cgcagctttg tttctgtgca aagttctttg tgtatgtatc ctatttttaa atttggcagt gaagagcaaa aaaaacgcta cctgccagag atggctgcgg ggaatattat aggttgcttt ggattaactg agccagactc aggatctgac ccggccagca tgcgtactca tgccaaaaaa attgaaggtg gctggatttt gaatggtgct aaaatgtgga tcaccaatgc ccctatcgca gacatcgcaa tagtgtgggc aaaaacagag gcgggaattc gtggttttat tgtcgataca aaatctgaag gcatgagttg tcctgaaatt aagttaaaga tgtctttacg agcctccatt accggggagc tggtttttga taatgttttt gttccagacg agaatttatt gccgaaaagt gaaaaagggc ttggagctgc gttaagttgc ttgagtcagg cgcgttatgg tatcgcatgg ggagcaatgg gcgctgccat ggcttgcttt gatatcgctc gggattattt gctggaacgc aagcagtttg ccaaaccttt ggcttcgttt cagttgattc aaaaagattt ggctgatatg tatacagaaa ttattaaagc acaatgcctt aatttgcaga ttgggcgttt aaaggatcag catcgtgaga caccgactat gatttctctg gctaagggga atgcttgccg tgaagcattg aaaattgcga gaaaatgtag gaatctaatg ggcgggaatg gaattagtct tgagtatcat gtcattcggc atatgctgaa tctggagtcg gtcttcactt atgaaggtac cgataatgtg catactttgg tcttgggaag acatataacg ggaattaatg cttttggttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LepnA.00027.a.B1.PS38392
PURIFICATION DATe: 12/12/2017
CONCENTRATION: 60.9mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHXX XDLLFLDEQL HDDERMIRDS VSRFVSNDVI PLMAESYEHA HFPKELIKKS ADLGLLGLTL PAEYGGAEAS YVAYGLVCQE LERGDSGLRS FVSVQSSLCM YPIFKFGSEE QKKRYLPEMA AGNIIGCFGL TEPDSGSDPA SMRTHAKKIE GGWILNGAKM WITNAPIADI AIVWAKTEAG IRGFIVDTKS EGMSCPEIKL KMSLRASITG ELVFDNVFVP DENLLPKSEK GLGAALSCLS QARYGIAWGA MGAAMACFDI ARDYLLERKQ FAKPLASFQL IQKDLADMYT EIIKAQCLNL QIGRLKDQHR ETPTMISLAK GNACREALKI ARKCRNLMGG NGISLEYHVI RHMLNLESVF TYEGTDNVHT LVLGRH
Validated NT Sequence
atggctcacc accaccacca ccatatnnnn nnngacttgt tgtttcttga tgaacaatta catgatgacg agcgcatgat acgagatagt gtgtctcgtt ttgttagcaa tgacgtaata cctttaatgg ctgaatctta tgagcacgcc catttcccaa aagagctgat aaaaaaatct gctgatctgg ggttgttggg cttaacttta cctgcggaat atgggggtgc agaagcttct tatgttgcct atggtttagt atgccaggaa ttggagcgag gtgatagcgg tttgcgcagc tttgtttctg tgcaaagttc tttgtgtatg tatcctattt ttaaatttgg cagtgaagag caaaaaaaac gctacctgcc agagatggct gcggggaata ttataggttg ctttggatta actgagccag actcaggatc tgacccggcc agcatgcgta ctcatgccaa aaaaattgaa ggtggctgga ttttgaatgg tgctaaaatg tggatcacca atgcccctat cgcagacatc gcaatagtgt gggcaaaaac agaggcggga attcgtggtt ttattgtcga tacaaaatct gaaggcatga gttgtcctga aattaagtta aagatgtctt tacgagcctc cattaccggg gagctggttt ttgataatgt ttttgttcca gacgagaatt tattgccgaa aagtgaaaaa gggcttggag ctgcgttaag ttgcttgagt caggcgcgtt atggtatcgc atggggagca atgggcgctg ccatggcttg ctttgatatc gctcgggatt atttgctgga acgcaagcag tttgccaaac ctttggcttc gtttcagttg attcaaaaag atttggctga tatgtataca gaaattatta aagcacaatg ccttaatttg cagattgggc gtttaaagga tcagcatcgt gagacaccga ctatgatttc tctggctaag gggaatgctt gccgtgaagc attgaaaatt gcgagaaaat gtaggaatct aatgggcggg aatggaatta gtcttgagta tcatgtcatt cggcatatgc tgaatctgga gtcggtcttc acttatgaag gtaccgataa tgtgcatact ttggtcttgg gaagacatat a
Expressed Protein Sequence
MAHHHHHHMD DLLFLDEQLH DDERMIRDSV SRFVSNDVIP LMAESYEHAH FPKELIKKSA DLGLLGLTLP AEYGGAEASY VAYGLVCQEL ERGDSGLRSF VSVQSSLCMY PIFKFGSEEQ KKRYLPEMAA GNIIGCFGLT EPDSGSDPAS MRTHAKKIEG GWILNGAKMW ITNAPIADIA IVWAKTEAGI RGFIVDTKSE GMSCPEIKLK MSLRASITGE LVFDNVFVPD ENLLPKSEKG LGAALSCLSQ ARYGIAWGAM GAAMACFDIA RDYLLERKQF AKPLASFQLI QKDLADMYTE IIKAQCLNLQ IGRLKDQHRE TPTMISLAKG NACREALKIA RKCRNLMGGN GISLEYHVIR HMLNLESVFT YEGTDNVHTL VLGRHITGIN AFG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGACG ACTTGTTGTT TCTTGATGAA CAATTACATG ATGACGAGCG CATGATACGA GATAGTGTGT CTCGTTTTGT TAGCAATGAC GTAATACCTT TAATGGCTGA ATCTTATGAG CACGCCCATT TCCCAAAAGA GCTGATAAAA AAATCTGCTG ATCTGGGGTT GTTGGGCTTA ACTTTACCTG CGGAATATGG GGGTGCAGAA GCTTCTTATG TTGCCTATGG TTTAGTATGC CAGGAATTGG AGCGAGGTGA TAGCGGTTTG CGCAGCTTTG TTTCTGTGCA AAGTTCTTTG TGTATGTATC CTATTTTTAA ATTTGGCAGT GAAGAGCAAA AAAAACGCTA CCTGCCAGAG ATGGCTGCGG GGAATATTAT AGGTTGCTTT GGATTAACTG AGCCAGACTC AGGATCTGAC CCGGCCAGCA TGCGTACTCA TGCCAAAAAA ATTGAAGGTG GCTGGATTTT GAATGGTGCT AAAATGTGGA TCACCAATGC CCCTATCGCA GACATCGCAA TAGTGTGGGC AAAAACAGAG GCGGGAATTC GTGGTTTTAT TGTCGATACA AAATCTGAAG GCATGAGTTG TCCTGAAATT AAGTTAAAGA TGTCTTTACG AGCCTCCATT ACCGGGGAGC TGGTTTTTGA TAATGTTTTT GTTCCAGACG AGAATTTATT GCCGAAAAGT GAAAAAGGGC TTGGAGCTGC GTTAAGTTGC TTGAGTCAGG CGCGTTATGG TATCGCATGG GGAGCAATGG GCGCTGCCAT GGCTTGCTTT GATATCGCTC GGGATTATTT GCTGGAACGC AAGCAGTTTG CCAAACCTTT GGCTTCGTTT CAGTTGATTC AAAAAGATTT GGCTGATATG TATACAGAAA TTATTAAAGC ACAATGCCTT AATTTGCAGA TTGGGCGTTT AAAGGATCAG CATCGTGAGA CACCGACTAT GATTTCTCTG GCTAAGGGGA ATGCTTGCCG TGAAGCATTG AAAATTGCGA GAAAATGTAG GAATCTAATG GGCGGGAATG GAATTAGTCT TGAGTATCAT GTCATTCGGC ATATGCTGAA TCTGGAGTCG GTCTTCACTT ATGAAGGTAC CGATAATGTG CATACTTTGG TCTTGGGAAG ACATATAACG GGAATTAATG CTTTTGGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT