LepnA.00023.a
Inorganic pyrophosphatase
| CENTER ID: | LepnA.00023.a |
| ORGANISM: | Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00023.a.B1.GE41290 | Full length(LepnA.00023.a) | 1 | 178 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| LepnA.00023.a.B1.PS38421 | Full length(LepnA.00023.a) | 1 | 178 |
Structures
6N1C
DEPOSITED: 11/8/2018
DETERMINATION: XRay
CLONE: LepnA.00023.a.B1.GE41290
PROTEIN: LepnA.00023.a.B1.PS38421
Publications by SSGCID
Characterization of a family I inorganic pyrophosphatase from Legionella
pneumophila
Abendroth JA, Barrett LK, Edwards TE, Lorimer DD, Myler PJ, Staker BL, Van Voorhis WC, Craig JC, Mayclin SJ, McLaughlin KJ, Barrett KF, Moorefield J, Konuk Y, Norman JO
Acta Crystallogr F Struct Biol Commun - 2023
volume F79, issue Part 10, pages 257-266
PMID: 37728609
PDB:
6N1C
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|272624.6.peg.2946 |
| UniProt: | Q5ZRW2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLMEIQSGR DVPNEVNVII EIPMHGEPVK YEVDKKTGAL FVDRFMTTAM FYPTNYGYIP NTLSEDGDPV DVLVITPVPL ISGAVISCRA VGMLKMTDES GVDAKILAVP TTKLSKMYQS MQTYQDIPQH LLLSIEHFFK HYKDLEEGKW VKVEGWVGPD AAREEITSSI NRYNHTKK
NT Sequence
ATGAGTTTAA TGGAAATTCA GAGCGGTCGT GATGTTCCAA ATGAAGTGAA TGTGATCATA GAAATCCCTA TGCACGGAGA ACCTGTAAAA TATGAAGTTG ATAAAAAAAC TGGTGCTTTA TTTGTAGATC GTTTTATGAC GACAGCAATG TTTTATCCAA CGAATTACGG TTATATCCCG AATACTCTTT CAGAAGATGG GGATCCTGTG GATGTGTTGG TAATAACTCC TGTCCCTTTG ATCAGTGGGG CTGTTATTTC ATGTCGAGCT GTTGGCATGT TAAAGATGAC AGATGAATCC GGAGTTGATG CAAAGATTTT GGCTGTTCCA ACAACCAAAC TAAGTAAAAT GTATCAATCC ATGCAAACTT ATCAAGATAT TCCTCAGCAT TTATTGTTGT CTATCGAACA TTTTTTTAAG CACTATAAAG ACCTGGAAGA AGGTAAATGG GTTAAGGTTG AGGGCTGGGT AGGCCCAGAT GCAGCTCGTG AGGAAATAAC ATCCAGTATT AACCGTTATA ATCACACAAA AAAG
Details for LepnA.00023.a.B1.GE41290
| HARVESTED ON: | 3/10/2017 |
| SEQUENCED ON: | 3/9/2017 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 21kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS LMEIQSGRDV PNEVNVIIEI PMHGEPVKYE VDKKTGALFV DRFMTTAMFY PTNYGYIPNT LSEDGDPVDV LVITPVPLIS GAVISCRAVG MLKMTDESGV DAKILAVPTT KLSKMYQSMQ TYQDIPQHLL LSIEHFFKHY KDLEEGKWVK VEGWVGPDAA REEITSSINR YNHTKK
Validated NT Sequence
atggctcacc accaccacca ccatatgagt ttaatggaaa ttcagagcgg tcgtgatgtt ccaaatgaag tgaatgtgat catagaaatc cctatgcacg gagaacctgt aaaatatgaa gttgataaaa aaactggtgc tttatttgta gatcgtttta tgacgacagc aatgttttat ccaacgaatt acggttatat cccgaatact ctttcagaag atggggatcc tgtggatgtg ttggtaataa ctcctgtccc tttgatcagt ggggctgtta tttcatgtcg agctgttggc atgttaaaga tgacagatga atccggagtt gatgcaaaga ttttggctgt tccaacaacc aaactaagta aaatgtatca atccatgcaa acttatcaag atattcctca gcatttattg ttgtctatcg aacatttttt taagcactat aaagacctgg aagaaggtaa atgggttaag gttgagggct gggtaggccc agatgcagct cgtgaggaaa taacatccag tattaaccgt tataatcaca caaaaaagta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMS LMEIQSGRDV PNEVNVIIEI PMHGEPVKYE VDKKTGALFV DRFMTTAMFY PTNYGYIPNT LSEDGDPVDV LVITPVPLIS GAVISCRAVG MLKMTDESGV DAKILAVPTT KLSKMYQSMQ TYQDIPQHLL LSIEHFFKHY KDLEEGKWVK VEGWVGPDAA REEITSSINR YNHTKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt taatggaaat tcagagcggt cgtgatgttc caaatgaagt gaatgtgatc atagaaatcc ctatgcacgg agaacctgta aaatatgaag ttgataaaaa aactggtgct ttatttgtag atcgttttat gacgacagca atgttttatc caacgaatta cggttatatc ccgaatactc tttcagaaga tggggatcct gtggatgtgt tggtaataac tcctgtccct ttgatcagtg gggctgttat ttcatgtcga gctgttggca tgttaaagat gacagatgaa tccggagttg atgcaaagat tttggctgtt ccaacaacca aactaagtaa aatgtatcaa tccatgcaaa cttatcaaga tattcctcag catttattgt tgtctatcga acattttttt aagcactata aagacctgga agaaggtaaa tgggttaagg ttgagggctg ggtaggccca gatgcagctc gtgaggaaat aacatccagt attaaccgtt ataatcacac aaaaaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for LepnA.00023.a.B1.PS38421
| PURIFICATION DATe: | 2/27/2018 |
| CONCENTRATION: | 42.55mg/ml |
| OBSERVED MW: | 21kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LMEIQSGRDV PNEVNVIIEI PMHGEPVKYE VDKKTGALFV DRFMTTAMFY PTNYGYIPNT LSEDGDPVDV LVITPVPLIS GAVISCRAVG MLKMTDESGV DAKILAVPTT KLSKMYQSMQ TYQDIPQHLL LSIEHFFKHY KDLEEGKWVK VEGWVGPDAA REEITSSINR YNHTKK
Validated NT Sequence
atggctcacc accaccacca ccatatgagt ttaatggaaa ttcagagcgg tcgtgatgtt ccaaatgaag tgaatgtgat catagaaatc cctatgcacg gagaacctgt aaaatatgaa gttgataaaa aaactggtgc tttatttgta gatcgtttta tgacgacagc aatgttttat ccaacgaatt acggttatat cccgaatact ctttcagaag atggggatcc tgtggatgtg ttggtaataa ctcctgtccc tttgatcagt ggggctgtta tttcatgtcg agctgttggc atgttaaaga tgacagatga atccggagtt gatgcaaaga ttttggctgt tccaacaacc aaactaagta aaatgtatca atccatgcaa acttatcaag atattcctca gcatttattg ttgtctatcg aacatttttt taagcactat aaagacctgg aagaaggtaa atgggttaag gttgagggct gggtaggccc agatgcagct cgtgaggaaa taacatccag tattaaccgt tataatcaca caaaaaagta agtgagtaag at
Expressed Protein Sequence
MAHHHHHHMS LMEIQSGRDV PNEVNVIIEI PMHGEPVKYE VDKKTGALFV DRFMTTAMFY PTNYGYIPNT LSEDGDPVDV LVITPVPLIS GAVISCRAVG MLKMTDESGV DAKILAVPTT KLSKMYQSMQ TYQDIPQHLL LSIEHFFKHY KDLEEGKWVK VEGWVGPDAA REEITSSINR YNHTKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT TAATGGAAAT TCAGAGCGGT CGTGATGTTC CAAATGAAGT GAATGTGATC ATAGAAATCC CTATGCACGG AGAACCTGTA AAATATGAAG TTGATAAAAA AACTGGTGCT TTATTTGTAG ATCGTTTTAT GACGACAGCA ATGTTTTATC CAACGAATTA CGGTTATATC CCGAATACTC TTTCAGAAGA TGGGGATCCT GTGGATGTGT TGGTAATAAC TCCTGTCCCT TTGATCAGTG GGGCTGTTAT TTCATGTCGA GCTGTTGGCA TGTTAAAGAT GACAGATGAA TCCGGAGTTG ATGCAAAGAT TTTGGCTGTT CCAACAACCA AACTAAGTAA AATGTATCAA TCCATGCAAA CTTATCAAGA TATTCCTCAG CATTTATTGT TGTCTATCGA ACATTTTTTT AAGCACTATA AAGACCTGGA AGAAGGTAAA TGGGTTAAGG TTGAGGGCTG GGTAGGCCCA GATGCAGCTC GTGAGGAAAT AACATCCAGT ATTAACCGTT ATAATCACAC AAAAAAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT