LepnA.00008.a

Serine hydroxymethyltransferase

CENTER ID: LepnA.00008.a
ORGANISM: Legionella pneumophila Philadelphia 1 / ATCC 33152 / DSM 7513
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LepnA.00008.a.B1.GE41287 Full length(LepnA.00008.a) 1 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
LepnA.00008.a.B1.PS38384 Full length(LepnA.00008.a) 1 417

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|272624.6.peg.747
UniProt: Q5ZXK6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDESYSIKN FDDVLFKAIS DEKRRQEEHI ELIASENYVS PRVLEAQGSV LTNKYAEGYP GKRYYGGCEF VDVAEELAIS RAKLLFGAHY VNVQPHSGSQ ANAAVMMALL SPGDTFMGMA LPHGGHLTHG SKVNFSGKLY HSVEYGVDSN TGLIDYDALE KLALQHKPKL IIAGFSAYSR ILDWARFREI ADKVGAYLMA DIAHVAGLVA VGLYPSPVPY ADVVTTTTHK TLRGPRGGLI LCKENEEIEK KLNSSVFPGM QGGPLMHVIA AKAVAFAEAL LPEFKTYQQQ VLANARTMCS VLQSRGYDIV SGGTDNHLLL VDLINKGITG KEADAAVGRA NITVNKNSVP NDPRSPFVTS GLRLGTPAAT TRGFKEREIT LLSNWVADVL DNVHDETNIS RVKTQVLLLC REFPVYA
NT Sequence
ATGTTTGACG AAAGTTATAG TATAAAAAAT TTTGATGATG TTCTTTTTAA AGCAATTTCT GATGAAAAAC GACGCCAAGA AGAACACATT GAATTAATAG CATCTGAAAA TTATGTCAGC CCGAGAGTAT TGGAGGCTCA GGGTTCTGTG TTGACGAATA AATACGCTGA AGGATATCCA GGCAAACGCT ATTACGGAGG TTGTGAGTTT GTTGATGTCG CTGAGGAGTT AGCCATTTCA AGGGCAAAAT TACTTTTTGG CGCTCATTAT GTCAATGTGC AACCGCATTC GGGATCACAA GCGAACGCAG CAGTCATGAT GGCATTATTG TCCCCAGGCG ATACTTTTAT GGGCATGGCT TTACCTCATG GTGGGCACTT GACTCATGGG TCAAAGGTTA ATTTTTCAGG TAAATTATAC CATTCTGTCG AGTATGGTGT TGATAGCAAT ACGGGTTTGA TTGATTATGA TGCTCTGGAA AAACTTGCGC TTCAACACAA ACCAAAATTG ATCATTGCCG GTTTTTCAGC TTATTCCAGG ATCCTGGATT GGGCTCGATT TAGAGAAATT GCCGATAAGG TCGGCGCTTA TTTAATGGCG GACATCGCTC ATGTAGCTGG TCTGGTGGCT GTAGGGTTAT ATCCTTCTCC TGTTCCTTAT GCGGATGTTG TGACAACGAC AACTCATAAA ACTTTAAGAG GACCTCGCGG TGGTTTAATC CTTTGTAAGG AAAACGAAGA AATTGAGAAA AAATTAAATT CTTCTGTATT TCCTGGTATG CAAGGTGGGC CCTTAATGCA TGTAATTGCA GCCAAGGCAG TTGCTTTTGC AGAAGCTCTG TTACCCGAAT TCAAAACTTA TCAGCAACAG GTTTTAGCTA ATGCCAGAAC GATGTGCAGT GTGTTACAAA GCAGAGGCTA TGATATTGTT TCTGGCGGAA CTGATAATCA TCTTTTGCTA GTGGATTTGA TAAATAAAGG AATTACAGGT AAAGAAGCTG ACGCTGCTGT AGGCAGAGCT AACATAACAG TCAATAAAAA TTCGGTTCCC AATGATCCTC GCTCGCCTTT TGTAACGAGT GGTTTACGTT TGGGTACGCC TGCTGCAACC ACAAGAGGCT TTAAAGAAAG AGAGATTACT TTGTTATCTA ACTGGGTAGC GGATGTCCTT GATAATGTTC ATGATGAAAC TAATATTTCA AGAGTGAAAA CCCAGGTGTT GCTACTCTGT CGTGAATTTC CGGTTTATGC C
Details for LepnA.00008.a.B1.GE41287
HARVESTED ON: 3/10/2017
SEQUENCED ON: 3/9/2017
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHXX XESYSIKNFD DVLFKAISDE KRRQEEHIEL IASENYVSPR VLEAQGSVLT NKYAEGYPGK RYYGGCEFVD VAEELAISRA KLLFGAHYVN VQPHSGSQAN AAVMMALLSP GDTFMGMALP HGGHLTHGSK VNFSGKLYHS VEYGVDSNTG LIDYDALEKL ALQHKPKLII AGFSAYSRIL DWARFREIAD KVGAYLMADI AHVAGLVAVG LYPSPVPYAD VVTTTTHKTL RGPRGGLILC KENEEIEKKL NSSVFPGMQG GPLMHVIAAK AVAFAEALLP EFKTYQQQVL ANARTMCSVL QSRGYDIVSG GTDNHLLLVD LINKGITGKE ADAAVGRANI TVNKNSVPND PRSPFVTSGL RLGTPAATTR GFKEREITLL SNWVADVLDN VHDETNISRV KTQVLL
Validated NT Sequence
atggctcacc accaccacca ccatatnnnn nnngaaagtt atagtataaa aaattttgat gatgttcttt ttaaagcaat ttctgatgaa aaacgacgcc aagaagaaca cattgaatta atagcatctg aaaattatgt cagcccgaga gtattggagg ctcagggttc tgtgttgacg aataaatacg ctgaaggata tccaggcaaa cgctattacg gaggttgtga gtttgttgat gtcgctgagg agttagccat ttcaagggca aaattacttt ttggcgctca ttatgtcaat gtgcaaccgc attcgggatc acaagcgaac gcagcagtca tgatggcatt attgtcccca ggcgatactt ttatgggcat ggctttacct catggtgggc acttgactca tgggtcaaag gttaattttt caggtaaatt ataccattct gtcgagtatg gtgttgatag caatacgggt ttgattgatt atgatgctct ggaaaaactt gcgcttcaac acaaaccaaa attgatcatt gccggttttt cagcttattc caggatcctg gattgggctc gatttagaga aattgccgat aaggtcggcg cttatttaat ggcggacatc gctcatgtag ctggtctggt ggctgtaggg ttatatcctt ctcctgttcc ttatgcggat gttgtgacaa cgacaactca taaaacttta agaggacctc gcggtggttt aatcctttgt aaggaaaacg aagaaattga gaaaaaatta aattcttctg tatttcctgg tatgcaaggt gggcccttaa tgcatgtaat tgcagccaag gcagttgctt ttgcagaagc tctgttaccc gaattcaaaa cttatcagca acaggtttta gctaatgcca gaacgatgtg cagtgtgtta caaagcagag gctatgatat tgtttctggc ggaactgata atcatctttt gctagtggat ttgataaata aaggaattac aggtaaagaa gctgacgctg ctgtaggcag agctaacata acagtcaata aaaattcggt tcccaatgat cctcgctcgc cttttgtaac gagtggttta cgtttgggta cgcctgctgc aaccacaaga ggctttaaag aaagagagat tactttgtta tctaactggg tagcggatgt ccttgataat gttcatgatg aaactaatat ttcaagagtg aaaacccagg tgttgctact
Expected Protein Sequence
MAHHHHHHMF DESYSIKNFD DVLFKAISDE KRRQEEHIEL IASENYVSPR VLEAQGSVLT NKYAEGYPGK RYYGGCEFVD VAEELAISRA KLLFGAHYVN VQPHSGSQAN AAVMMALLSP GDTFMGMALP HGGHLTHGSK VNFSGKLYHS VEYGVDSNTG LIDYDALEKL ALQHKPKLII AGFSAYSRIL DWARFREIAD KVGAYLMADI AHVAGLVAVG LYPSPVPYAD VVTTTTHKTL RGPRGGLILC KENEEIEKKL NSSVFPGMQG GPLMHVIAAK AVAFAEALLP EFKTYQQQVL ANARTMCSVL QSRGYDIVSG GTDNHLLLVD LINKGITGKE ADAAVGRANI TVNKNSVPND PRSPFVTSGL RLGTPAATTR GFKEREITLL SNWVADVLDN VHDETNISRV KTQVLLLCRE FPVYA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg acgaaagtta tagtataaaa aattttgatg atgttctttt taaagcaatt tctgatgaaa aacgacgcca agaagaacac attgaattaa tagcatctga aaattatgtc agcccgagag tattggaggc tcagggttct gtgttgacga ataaatacgc tgaaggatat ccaggcaaac gctattacgg aggttgtgag tttgttgatg tcgctgagga gttagccatt tcaagggcaa aattactttt tggcgctcat tatgtcaatg tgcaaccgca ttcgggatca caagcgaacg cagcagtcat gatggcatta ttgtccccag gcgatacttt tatgggcatg gctttacctc atggtgggca cttgactcat gggtcaaagg ttaatttttc aggtaaatta taccattctg tcgagtatgg tgttgatagc aatacgggtt tgattgatta tgatgctctg gaaaaacttg cgcttcaaca caaaccaaaa ttgatcattg ccggtttttc agcttattcc aggatcctgg attgggctcg atttagagaa attgccgata aggtcggcgc ttatttaatg gcggacatcg ctcatgtagc tggtctggtg gctgtagggt tatatccttc tcctgttcct tatgcggatg ttgtgacaac gacaactcat aaaactttaa gaggacctcg cggtggttta atcctttgta aggaaaacga agaaattgag aaaaaattaa attcttctgt atttcctggt atgcaaggtg ggcccttaat gcatgtaatt gcagccaagg cagttgcttt tgcagaagct ctgttacccg aattcaaaac ttatcagcaa caggttttag ctaatgccag aacgatgtgc agtgtgttac aaagcagagg ctatgatatt gtttctggcg gaactgataa tcatcttttg ctagtggatt tgataaataa aggaattaca ggtaaagaag ctgacgctgc tgtaggcaga gctaacataa cagtcaataa aaattcggtt cccaatgatc ctcgctcgcc ttttgtaacg agtggtttac gtttgggtac gcctgctgca accacaagag gctttaaaga aagagagatt actttgttat ctaactgggt agcggatgtc cttgataatg ttcatgatga aactaatatt tcaagagtga aaacccaggt gttgctactc tgtcgtgaat ttccggttta tgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LepnA.00008.a.B1.PS38384
PURIFICATION DATe: 11/21/2017
CONCENTRATION: 23.3mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHXX XESYSIKNFD DVLFKAISDE KRRQEEHIEL IASENYVSPR VLEAQGSVLT NKYAEGYPGK RYYGGCEFVD VAEELAISRA KLLFGAHYVN VQPHSGSQAN AAVMMALLSP GDTFMGMALP HGGHLTHGSK VNFSGKLYHS VEYGVDSNTG LIDYDALEKL ALQHKPKLII AGFSAYSRIL DWARFREIAD KVGAYLMADI AHVAGLVAVG LYPSPVPYAD VVTTTTHKTL RGPRGGLILC KENEEIEKKL NSSVFPGMQG GPLMHVIAAK AVAFAEALLP EFKTYQQQVL ANARTMCSVL QSRGYDIVSG GTDNHLLLVD LINKGITGKE ADAAVGRANI TVNKNSVPND PRSPFVTSGL RLGTPAATTR GFKEREITLL SNWVADVLDN VHDETNISRV KTQVLL
Validated NT Sequence
atggctcacc accaccacca ccatatnnnn nnngaaagtt atagtataaa aaattttgat gatgttcttt ttaaagcaat ttctgatgaa aaacgacgcc aagaagaaca cattgaatta atagcatctg aaaattatgt cagcccgaga gtattggagg ctcagggttc tgtgttgacg aataaatacg ctgaaggata tccaggcaaa cgctattacg gaggttgtga gtttgttgat gtcgctgagg agttagccat ttcaagggca aaattacttt ttggcgctca ttatgtcaat gtgcaaccgc attcgggatc acaagcgaac gcagcagtca tgatggcatt attgtcccca ggcgatactt ttatgggcat ggctttacct catggtgggc acttgactca tgggtcaaag gttaattttt caggtaaatt ataccattct gtcgagtatg gtgttgatag caatacgggt ttgattgatt atgatgctct ggaaaaactt gcgcttcaac acaaaccaaa attgatcatt gccggttttt cagcttattc caggatcctg gattgggctc gatttagaga aattgccgat aaggtcggcg cttatttaat ggcggacatc gctcatgtag ctggtctggt ggctgtaggg ttatatcctt ctcctgttcc ttatgcggat gttgtgacaa cgacaactca taaaacttta agaggacctc gcggtggttt aatcctttgt aaggaaaacg aagaaattga gaaaaaatta aattcttctg tatttcctgg tatgcaaggt gggcccttaa tgcatgtaat tgcagccaag gcagttgctt ttgcagaagc tctgttaccc gaattcaaaa cttatcagca acaggtttta gctaatgcca gaacgatgtg cagtgtgtta caaagcagag gctatgatat tgtttctggc ggaactgata atcatctttt gctagtggat ttgataaata aaggaattac aggtaaagaa gctgacgctg ctgtaggcag agctaacata acagtcaata aaaattcggt tcccaatgat cctcgctcgc cttttgtaac gagtggttta cgtttgggta cgcctgctgc aaccacaaga ggctttaaag aaagagagat tactttgtta tctaactggg tagcggatgt ccttgataat gttcatgatg aaactaatat ttcaagagtg aaaacccagg tgttgctact
Expressed Protein Sequence
MAHHHHHHMF DESYSIKNFD DVLFKAISDE KRRQEEHIEL IASENYVSPR VLEAQGSVLT NKYAEGYPGK RYYGGCEFVD VAEELAISRA KLLFGAHYVN VQPHSGSQAN AAVMMALLSP GDTFMGMALP HGGHLTHGSK VNFSGKLYHS VEYGVDSNTG LIDYDALEKL ALQHKPKLII AGFSAYSRIL DWARFREIAD KVGAYLMADI AHVAGLVAVG LYPSPVPYAD VVTTTTHKTL RGPRGGLILC KENEEIEKKL NSSVFPGMQG GPLMHVIAAK AVAFAEALLP EFKTYQQQVL ANARTMCSVL QSRGYDIVSG GTDNHLLLVD LINKGITGKE ADAAVGRANI TVNKNSVPND PRSPFVTSGL RLGTPAATTR GFKEREITLL SNWVADVLDN VHDETNISRV KTQVLLLCRE FPVYA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG ACGAAAGTTA TAGTATAAAA AATTTTGATG ATGTTCTTTT TAAAGCAATT TCTGATGAAA AACGACGCCA AGAAGAACAC ATTGAATTAA TAGCATCTGA AAATTATGTC AGCCCGAGAG TATTGGAGGC TCAGGGTTCT GTGTTGACGA ATAAATACGC TGAAGGATAT CCAGGCAAAC GCTATTACGG AGGTTGTGAG TTTGTTGATG TCGCTGAGGA GTTAGCCATT TCAAGGGCAA AATTACTTTT TGGCGCTCAT TATGTCAATG TGCAACCGCA TTCGGGATCA CAAGCGAACG CAGCAGTCAT GATGGCATTA TTGTCCCCAG GCGATACTTT TATGGGCATG GCTTTACCTC ATGGTGGGCA CTTGACTCAT GGGTCAAAGG TTAATTTTTC AGGTAAATTA TACCATTCTG TCGAGTATGG TGTTGATAGC AATACGGGTT TGATTGATTA TGATGCTCTG GAAAAACTTG CGCTTCAACA CAAACCAAAA TTGATCATTG CCGGTTTTTC AGCTTATTCC AGGATCCTGG ATTGGGCTCG ATTTAGAGAA ATTGCCGATA AGGTCGGCGC TTATTTAATG GCGGACATCG CTCATGTAGC TGGTCTGGTG GCTGTAGGGT TATATCCTTC TCCTGTTCCT TATGCGGATG TTGTGACAAC GACAACTCAT AAAACTTTAA GAGGACCTCG CGGTGGTTTA ATCCTTTGTA AGGAAAACGA AGAAATTGAG AAAAAATTAA ATTCTTCTGT ATTTCCTGGT ATGCAAGGTG GGCCCTTAAT GCATGTAATT GCAGCCAAGG CAGTTGCTTT TGCAGAAGCT CTGTTACCCG AATTCAAAAC TTATCAGCAA CAGGTTTTAG CTAATGCCAG AACGATGTGC AGTGTGTTAC AAAGCAGAGG CTATGATATT GTTTCTGGCG GAACTGATAA TCATCTTTTG CTAGTGGATT TGATAAATAA AGGAATTACA GGTAAAGAAG CTGACGCTGC TGTAGGCAGA GCTAACATAA CAGTCAATAA AAATTCGGTT CCCAATGATC CTCGCTCGCC TTTTGTAACG AGTGGTTTAC GTTTGGGTAC GCCTGCTGCA ACCACAAGAG GCTTTAAAGA AAGAGAGATT ACTTTGTTAT CTAACTGGGT AGCGGATGTC CTTGATAATG TTCATGATGA AACTAATATT TCAAGAGTGA AAACCCAGGT GTTGCTACTC TGTCGTGAAT TTCCGGTTTA TGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT