LemeA.20384.a

Protein kinase domain-containing protein

CENTER ID: LemeA.20384.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.20384.a.HZ2.GE43730 kinase domain (Jensen) 1 511
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.21.0270
UniProt: E9AUZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVDTVKGR YSSYKLLNEV GRGGSALVYR AQDVATRKDV AVKQLFGNRP QDMEDWLREV DALHTLNNHH CPHVVKYLDH MQQHDRLFLV EEFTEHGSLL RRLKENGKLP EDTACRYIYQ VLTALCHMAP WGVVHGDLKA SNILLFDGDV VKVTDFALRS HGEDDHEDAE RRTTAVSTPS VEAPAHMKGS RAGRGAGEEC SGSALSEFRG SAYWAAPEVL AGASKATAAS DIWSVGCLAV ELLTGAPPHF ERPIHNAIHH ILKSYYEVLC ESEASRVGAG GTSRASTNPA TPSAATGSGD DEGPRGPRRK KNGAKDACGA ASQKTDVASA RRATATTSTH NPPKEPVDVA KTAASGGAVH ASDQTEVATP LCEAALLPPL PEDLHLSDEC LSFLRMCFRP RGMDRPSAGE LLHEPWFLDC TVPQLLRAAR EGRPMNGTTG DESVSAGTSS GTRFGVIEQW VKRNLTCDNE SRCEAWLNSN ALPLLVPALT PRIMTPKYIG NVMWCFSHFA ESRSALATLF VNRLGSTELW GVEELTSACD ADHLATLFRR CCATQDAQVP VYTPTDPRAL RFVLGLEKEK VLACVRALHS RLVLEPTAAA MAEAVSSLTT TTDDTAVDLN DCSASSPDPA APAHTRAPDC APVSLQEQHE QQRRARERLL SDGGAAVLCQ CVEAQCKAAF LSNTTPMMEW STMNLLFDVL CTIEPLAGGQ ALLWGLGADF SGGHTSPSST VGAGGGAAVV KTLTVVVNPC GGRGGGELNS TCDKPSPATG APLLVSPISV SPQESILTTA APAGGGGGPL SSLGSTMGFG CHTVSGLPPE SVQWTTSMSW LLAVQEAARH LCEAAVRLLT RYIPVARRCR AEYLEKAGVS LTATLVLVGS SEVVSTEVRC AAVEGLPQLQ ASSLRAARYL RDPVRCIALL ALTLKRSYGV PALTSCLLTA ITAMTSEKQM LAACTGCPWV WEALVSLLRE VDAADKAAAA ADKRTSSLPH SAASTDAGAA EGADAPPALA AASPTSVKSP SASSSNGGGG SNGAAAAFAD IVVLLSRWFA EVTPTALLSS PVTAAAAASE PIPSLGHPAA SLSLPVLLQT LRRQLVALSQ NGRVCHGDLM PDVAKVLRHL APLEAAAAAA STSTAMVA
NT Sequence
atggccaccg tagacaccgt caaggggcgt tactcgtcct acaagctgct caacgaagtt ggccgtggtg gcagtgcgct cgtgtaccgc gcgcaggatg tggccacacg aaaagatgtt gccgtgaagc agctctttgg caaccggccg caggatatgg aagactggct gcgcgaggtg gacgctctac acacactcaa caaccatcac tgcccgcatg tggtgaagta cctggaccac atgcagcagc acgatcgact cttcctcgtg gaggagttca ccgagcatgg atcgctgctg cgacgtctga aggaaaacgg caagcttccg gaggacaccg cttgccggta tatctaccag gtgctgacgg ccctgtgtca catggccccg tggggcgtcg tgcacggtga cttgaaggcg tccaacattc ttctcttcga cggcgatgtc gtgaaagtga ccgactttgc actgcgttcg catggcgagg acgatcacga ggatgccgag cggcgcacca ccgcggtctc cacgccttct gtcgaagcac cggcccacat gaagggcagc cgcgccggga ggggcgctgg tgaagagtgc tctggctccg ccctctcgga gttccgcggc tccgcttact gggcagcgcc ggaagtcctc gccggtgcct cgaaggcaac ggcggcgagc gacatctggt cggtgggctg tctcgcggtg gagctgctca cgggcgcccc cccgcacttt gagcgcccca tccacaacgc cattcatcac atcctcaaga gctactacga agtgctctgt gagagcgagg ccagccgtgt aggcgcaggt ggcacctcgc gtgcgtccac caaccccgca accccaagcg cggcaacagg ctctggcgat gatgaagggc cgcgtgggcc ccggaggaag aaaaacggag cgaaagacgc gtgtggtgca gcgtctcaaa agacagacgt ggcgtccgcg aggagggcga cggcaacgac gagcactcac aatccgccga aggagccggt cgatgtggcc aagacagccg catctggcgg cgctgtgcat gcatcagacc agactgaagt ggccacgccc ctgtgcgagg cggcgctgct cccgccgctt cccgaggacc tgcacctgag cgatgagtgc ctgtcgtttc tacggatgtg cttccgcccc cgcgggatgg accggccgtc ggccggagag ctgctccacg agccgtggtt cctggactgc accgtaccgc agcttctgcg ggcggcgcgc gagggtcggc ctatgaacgg gacgacaggc gacgagagcg tcagcgccgg caccagcagt ggcacccgct ttggggtgat tgagcaatgg gtgaagcgta acctgacatg tgacaacgag agccggtgcg aggcgtggct gaacagcaac gcactgccgc tgctggtgcc ggcgctgacg ccgcgtatta tgacacccaa gtacatagga aacgtgatgt ggtgtttttc ccactttgcc gagagccgct cagccttggc gacgctcttt gtgaaccggt tggggtcgac agagctctgg ggggtggagg agctgacgtc ggcgtgcgac gcggaccacc tcgccacact ctttcggcgg tgctgtgcaa cgcaggacgc acaagtcccc gtgtacaccc cgacagaccc gcgtgcgctg cgctttgtct tgggcctgga gaaggaaaag gttctggcat gcgtgcgcgc gttgcactcg cgtctcgtgc tcgagccaac ggccgcggcg atggcggagg ctgtgagctc gctgaccacc accacagacg acaccgcagt ggatctgaac gactgctccg cctcctcgcc tgaccccgcg gcgcccgcgc atacgcgcgc gccggattgc gcaccagtat cccttcaaga gcaacatgaa caacagcggc gtgcccgtga gcggctactc agcgacggcg gcgcggcggt gctgtgccag tgcgtggagg cgcagtgcaa ggccgctttc ctgagcaaca caacgcccat gatggagtgg agcacgatga acctgctgtt tgacgtgctc tgcaccatcg agccgctggc gggtgggcag gcgctgctgt ggggcctcgg cgctgacttc agcggtggcc acacctctcc gagctctaca gtgggcgcag gcggtggagc ggcggttgtg aagacgctga cggttgtcgt caacccgtgc gggggacgag ggggcggcga gttgaacagc acctgtgaca aaccctcgcc agcgacgggt gccccgcttt tggtgtcacc gatctccgtg agcccgcagg agtcgatcct gacgacagcg gcaccggcgg gtggcggtgg cggcccgctg agctccctag gcagcactat gggcttcggg tgccacaccg tcagtgggct tccaccggag tcagtgcagt ggaccacatc catgtcatgg ctgctcgcag tgcaagaggc ggcgcgccac ctgtgcgaag cagcggtgcg cctgctcacc cgctacatcc cagtcgccag aaggtgcagg gccgagtacc tcgaaaaggc cggcgttagt ctgaccgcaa cgctggtcct cgtgggctcg agcgaggtgg tcagcaccga ggtgcgatgc gcggctgtcg aaggcctgcc gcagctacag gcgagctcgc tccgtgcggc ccggtacctg cgcgacccgg tgcgctgcat tgccctgctg gcgctcaccc tgaagcggtc ctacggtgtc cctgcgctga catcgtgcct gctcacagcc atcaccgcca tgacgtccga gaagcagatg ctggcggcct gcaccggctg cccgtgggtg tgggaagcac tggtgtccct gctaagagaa gtggacgcag ctgacaaagc cgccgccgct gctgacaagc gcaccagctc gctcccccac agcgccgcca gcaccgacgc tggcgcggcc gagggggccg acgccccacc cgccttggcg gcggcgagtc ccacatccgt caaatcgcct agcgccagca gcagcaacgg cggtggtggc agcaacggcg ccgctgctgc ctttgcagac atcgttgttc tgctctcgcg ttggttcgcg gaggtgacac cgacagcgct gctgagcagt ccagtaaccg cagcagcagc ggcatcggag ccaatacctt ccctcggtca tccagcagcg tccctatccc tcccggtatt gctgcagaca cttcgccgcc agctcgtagc gctgtcgcag aacggtcgcg tctgccacgg ggatctgatg ccggacgtgg ccaaggtgct gcggcatttg gcccctctcg aagccgccgc cgccgccgcg tcaacgtcga cggccatggt ggcgtga
Details for LemeA.20384.a.HZ2.GE43730
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMA TVDTVKGRYS SYKLLNEVGR GGSALVYRAQ DVATRKDVAV KQLFGNRPQD MEDWLREVDA LHTLNNHHCP HVVKYLDHMQ QHDRLFLVEE FTEHGSLLRR LKENGKLPED TACRYIYQVL TALCHMAPWG VVHGDLKASN ILLFDGDVVK VTDFALRSHG EDDHEDAERR TTAVSTPSVE APAHMKGSRA GRGAGEECSG SALSEFRGSA YWAAPEVLAG ASKATAASDI WSVGCLAVEL LTGAPPHFER PIHNAIHHIL KSYYEVLCES EASRVGAGGT SRASTNPATP SAATGSGDDE GPRGPRRKKN GAKDACGAAS QKTDVASARR ATATTSTHNP PKEPVDVAKT AASGGAVHAS DQTEVATPLC EAALLPPLPE DLHLSDECLS FLRMCFRPRG MDRPSAGELL HEPWFLDCTV PQLLRAAREG RPMNGTTGDE SVSAGTSSGT RFGVIEQWVK RNLTCDNESR CEAWLNSNAL PLLVPALTPR IMTPKYIGNV MWCFSHFAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggccaccgt agacaccgtc aaggggcgtt actcgtccta caagctgctc aacgaagttg gccgtggtgg cagtgcgctc gtgtaccgcg cgcaggatgt ggccacacga aaagatgttg ccgtgaagca gctctttggc aaccggccgc aggatatgga agactggctg cgcgaggtgg acgctctaca cacactcaac aaccatcact gcccgcatgt ggtgaagtac ctggaccaca tgcagcagca cgatcgactc ttcctcgtgg aggagttcac cgagcatgga tcgctgctgc gacgtctgaa ggaaaacggc aagcttccgg aggacaccgc ttgccggtat atctaccagg tgctgacggc cctgtgtcac atggccccgt ggggcgtcgt gcacggtgac ttgaaggcgt ccaacattct tctcttcgac ggcgatgtcg tgaaagtgac cgactttgca ctgcgttcgc atggcgagga cgatcacgag gatgccgagc ggcgcaccac cgcggtctcc acgccttctg tcgaagcacc ggcccacatg aagggcagcc gcgccgggag gggcgctggt gaagagtgct ctggctccgc cctctcggag ttccgcggct ccgcttactg ggcagcgccg gaagtcctcg ccggtgcctc gaaggcaacg gcggcgagcg acatctggtc ggtgggctgt ctcgcggtgg agctgctcac gggcgccccc ccgcactttg agcgccccat ccacaacgcc attcatcaca tcctcaagag ctactacgaa gtgctctgtg agagcgaggc cagccgtgta ggcgcaggtg gcacctcgcg tgcgtccacc aaccccgcaa ccccaagcgc ggcaacaggc tctggcgatg atgaagggcc gcgtgggccc cggaggaaga aaaacggagc gaaagacgcg tgtggtgcag cgtctcaaaa gacagacgtg gcgtccgcga ggagggcgac ggcaacgacg agcactcaca atccgccgaa ggagccggtc gatgtggcca agacagccgc atctggcggc gctgtgcatg catcagacca gactgaagtg gccacgcccc tgtgcgaggc ggcgctgctc ccgccgcttc ccgaggacct gcacctgagc gatgagtgcc tgtcgtttct acggatgtgc ttccgccccc gcgggatgga ccggccgtcg gccggagagc tgctccacga gccgtggttc ctggactgca ccgtaccgca gcttctgcgg gcggcgcgcg agggtcggcc tatgaacggg acgacaggcg acgagagcgt cagcgccggc accagcagtg gcacccgctt tggggtgatt gagcaatggg tgaagcgtaa cctgacatgt gacaacgaga gccggtgcga ggcgtggctg aacagcaacg cactgccgct gctggtgccg gcgctgacgc cgcgtattat gacacccaag tacataggaa acgtgatgtg gtgtttttcc cactttgccg agtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa