LemeA.20227.a

protein kinase, putative (OPH8)

CENTER ID: LemeA.20227.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.20227.a.B2.GE42817 PHoranyi kinase domain truncation 450 753
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.25.1520.1
UniProt: E9AXN2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSSKRHAY EPLPQQQRPT NLPPADQHRR VSTGTGRESP ALPPLSPNSM PAVTNIAEPS SGPVLISTSA ASSTLVPIFQ GRAGSAAASR GGGDGNSEVH PRPAEVSQTY PGAFTTAPQS MALAVVPFTG TSGGGMGSDS VGVSAPPRCD PRVGSPDEEG KITGGGDDVG YYSGPSPYGK LAPLSLSTER VCPHCLRPYD VFDDGEGVFD PATFFCVGAM ARPSSMQPSP SHPKLLPPPS QEGNEQQNQK PSTSPRLYTD GAGDGGGAAG QLLYSRSTTA AGLEDSTSDG ANRSGNYITA VHPPAAAFTS HYFRALPPPS FIIAAGARGP SAPLAIEDYH PSPLLDSPRG EEEEVRQSFA LAPAPASDSG DGSGHSSGTL VCKVAAVPHY SYVWPITCTQ MRSQVQLLQQ DDGSEKTAAE VEGGKEESPS CESSSPAFSP TAGAPSPNNG YYRHYFKELR QLGRGTYGGV YLCRHIMCGV NLGEFALKKI PVGDKVTYLQ SVLREVRILE EVRRHPNVVE YKHSWVEEAQ LADFGPPVRC LFILMEYASA GSLDAYLERY GNNLSTLAVW YFFLSSVAGT AHLHEKHILH RDLKPQNLLL AAAKDRPPRV LVSDFGTAAL LDDILYDRSG GTGTLEYMAP ELLETTASPR GTNEQYVNHH TMASDVWSLG MVLHYLAFDG TLPERHEDGS VNLDKARHSA NARPPEMLRL LEAMLQLDPA KRPRCSDILG SPMMQSIMRI FNKDDHTQWD LSIQRQQQLS ATSSSAILPL ERPPRQPVPA PASRSTPLNV LEAPSIDDSR SPCGVPSRNT FAPISTSSQG RGVRANRTNM EDKSNALQSS RVELLSASTL VDLSTAMARA GSSSSSNGSP FTIKLPPPRP PSTAVSQHSR QQQQRAHVVA SLPQTLASFN TSQFSDSPSP RRVNVGVQTK PVVIVEEQQR HT
NT Sequence
atgaatccgt cgtcgaagcg gcacgcgtac gagccgctgc cgcagcagca gcgccccaca aacctgccgc ctgctgatca gcatcgccga gtaagcacag gcactggtcg tgagagccct gcccttccac cgctctcgcc gaactcgatg cctgcggtga ccaacatcgc cgagccctcc tcaggtcccg tcctcatctc cacctcggca gcgtcgtcga ctctggtgcc catttttcaa gggcgggcgg gttctgcagc cgcttcaaga ggtggcggtg acggcaattc tgaagtgcac cctcgtcccg cagaggtaag ccaaacctac cccggggcgt tcaccacggc cccgcagtcc atggcgctgg cggtggtgcc cttcacgggc actagcggtg gtgggatggg cagtgacagc gtcggcgtca gtgcaccgcc acgttgtgac ccgagagtgg gcagtcccga cgaggaaggg aagatcacgg gtggtggcga tgacgttggc tactactctg ggccctcccc gtatggcaag ctggccccgt tgtcgctgag cacggagcga gtgtgtccgc actgccttcg gccatacgat gtctttgacg acggcgaagg cgtcttcgat ccggcgacat tcttctgcgt gggtgcgatg gcgaggccat cgtcgatgca gccctcacca agccatccga aactcctccc gccgccgtcg caggagggga atgagcagca gaaccagaaa ccatcgacgt ccccgcgctt atacactgac ggcgcagggg atggtggcgg tgctgctggg cagctgttgt acagcaggag caccacggcc gctgggctcg aggatagcac cagtgatggc gccaaccgca gcggcaacta catcacagcc gtacatccac cagctgctgc gttcacctca cattacttcc gtgcgctccc cccaccctcg ttcatcatag cggcgggagc ccgcggccca tcggcaccgc ttgccatcga ggactaccac ccatcaccgc tgctcgatag cccgagaggc gaggaggagg aagtgagaca gtcgttcgca ctggctcccg cgcctgccag tgacagcggt gatggaagtg gtcactcaag tgggacgctg gtgtgcaaag tagctgccgt gccgcactac tcctacgtgt ggcccatcac ctgcacacag atgcgctcgc aggtgcaact gctgcagcag gatgacggca gcgagaagac ggcggcagag gtggagggcg ggaaagagga gtcgccgtcg tgcgaaagca gttcgccagc cttctctccc accgctgggg cgccgtcgcc gaacaatggc tactaccgtc attacttcaa ggagctccgt cagctgggcc gcggcaccta cggaggggtc tacctctgtc gccacattat gtgtggcgtt aacctcggcg agttcgctct taagaagatt ccagtcggtg acaaggtcac ctacctgcag tcggtgttgc gtgaggtgcg cattctcgag gaggtgcggc gacaccccaa cgtggtcgag tacaagcaca gctgggtgga ggaggcgcag ctcgccgact ttggcccgcc tgtgcgctgt ctcttcatct tgatggagta cgcttccgct ggctcgctcg acgcgtatct ggagcgctac ggcaacaatc tctccaccct cgccgtgtgg tacttcttcc tcagctccgt ggcaggcacg gctcaccttc acgagaagca cattttgcac cgcgacttaa agcctcagaa tctgttgctg gccgcggcga aggaccgtcc gccacgggtg ctggtcagcg actttggcac ggcggcgctg ctggatgaca tcttgtatga ccggtccggt ggcacgggca ccctcgagta catggcgccg gagctcctcg agacaaccgc ctctccacgc ggcaccaacg agcagtacgt aaaccatcac acaatggcct ccgacgtgtg gtcgctgggg atggtgctgc actacctcgc cttcgatggc acgctgccgg agcgacacga ggacggcagt gtcaacctcg acaaggcacg ccactcggcg aacgccagac cccctgaaat gctcagactg ctggaggcca tgttgcaact cgatcccgca aagcggccgc gctgcagcga catcctcggc tccccaatga tgcagtccat catgcgcatc ttcaacaagg atgaccatac ccagtgggac ctctccatcc agcggcagca gcagctctcg gctacctcgt cgtccgcgat tctcccgttg gagcgccccc ctcgccaacc ggtgccggca ccagcgtcca ggagtacacc actcaatgtt ctagaggccc ccagcatcga tgacagcaga tcgccctgcg gcgttcccag ccgcaacact tttgcgccca tctccacctc ttcacaggga cgtggcgtgc gggccaaccg cacaaatatg gaggacaaga gcaacgcact tcaaagcagc cgtgtcgagc tcctctcagc gtcaacgttg gtggatctgt caacggcgat ggcgcgtgcc gggagcagca gcagcagcaa cggtagcccc ttcacaataa agttgcctcc gccgcgccct ccatctactg cggtctccca gcatagccga cagcagcaac aacgcgcgca tgtagtggcc agtttacctc agaccctagc ctccttcaac acctcccagt tttcagactc accatcgcca cgcagggtca acgtcggcgt gcaaaccaaa ccggtggtga tagtggagga gcagcaacgg cacacataa
Details for LemeA.20227.a.B2.GE42817
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG YYRHYFKELR QLGRGTYGGV YLCRHIMCGV NLGEFALKKI PVGDKVTYLQ SVLREVRILE EVRRHPNVVE YKHSWVEEAQ LADFGPPVRC LFILMEYASA GSLDAYLERY GNNLSTLAVW YFFLSSVAGT AHLHEKHILH RDLKPQNLLL AAAKDRPPRV LVSDFGTAAL LDDILYDRSG GTGTLEYMAP ELLETTASPR GTNEQYVNHH TMASDVWSLG MVLHYLAFDG TLPERHEDGS VNLDKARHSA NARPPEMLRL LEAMLQLDPA KRPRCSDILG SPMMQSIMRI FNKDXXXXWD LSX
Validated NT Sequence
ttnnntggag aggtcccann nnnnnnnnnn atccttgttg aagatgcgca tgatggactg catcattggg gagccgagga tgtcgctgca gcgcggccgc tttgcgggat cgagttgcaa catggcctcc agcagtctga gcatttcagg gggtctggcg ttcgccgagt ggcgtgcctt gtcgaggttg acactgccgt cctcgtgtcg ctccggcagc gtgccatcga aggcgaggta gtgcagcacc atccccagcg accacacgtc ggaggccatt gtgtgatggt ttacgtactg ctcgttggtg ccgcgtggag aggcggttgt ctcgaggagc tccggcgcca tgtactcgag ggtgcccgtg ccaccggacc ggtcatacaa gatgtcatcc agcagcgccg ccgtgccaaa gtcgctgacc agcacccgtg gcggacggtc cttcgccgcg gccagcaaca gattctgagg ctttaagtcg cggtgcaaaa tgtgcttctc gtgaaggtga gccgtgcctg ccacggagct gaggaagaag taccacacgg cgagggtgga gagattgttg ccgtagcgct ccagatacgc gtcgagcgag ccagcggaag cgtactccat caagatgaag agacagcgca caggcgggcc aaagtcggcg agctgcgcct cctccaccca gctgtgcttg tactcgacca cgttggggtg tcgccgcacc tcctcgagaa tgcgcacctc acgcaacacc gactgcaggt aggtgacctt gtcaccgact ggaatcttct taagagcgaa ctcgccgagg ttaacgccac acataatgtg gcgacagagg tagacccctc cgtaggtgcc gcggcccagc tgacggagct ccttgaagta atgacggtag tagcccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHGY YRHYFKELRQ LGRGTYGGVY LCRHIMCGVN LGEFALKKIP VGDKVTYLQS VLREVRILEE VRRHPNVVEY KHSWVEEAQL ADFGPPVRCL FILMEYASAG SLDAYLERYG NNLSTLAVWY FFLSSVAGTA HLHEKHILHR DLKPQNLLLA AAKDRPPRVL VSDFGTAALL DDILYDRSGG TGTLEYMAPE LLETTASPRG TNEQYVNHHT MASDVWSLGM VLHYLAFDGT LPERHEDGSV NLDKARHSAN ARPPEMLRLL EAMLQLDPAK RPRCSDILGS PMMQSIMRIF NKDDHTQWDL SI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctact accgtcatta cttcaaggag ctccgtcagc tgggccgcgg cacctacgga ggggtctacc tctgtcgcca cattatgtgt ggcgttaacc tcggcgagtt cgctcttaag aagattccag tcggtgacaa ggtcacctac ctgcagtcgg tgttgcgtga ggtgcgcatt ctcgaggagg tgcggcgaca ccccaacgtg gtcgagtaca agcacagctg ggtggaggag gcgcagctcg ccgactttgg cccgcctgtg cgctgtctct tcatcttgat ggagtacgct tccgctggct cgctcgacgc gtatctggag cgctacggca acaatctctc caccctcgcc gtgtggtact tcttcctcag ctccgtggca ggcacggctc accttcacga gaagcacatt ttgcaccgcg acttaaagcc tcagaatctg ttgctggccg cggcgaagga ccgtccgcca cgggtgctgg tcagcgactt tggcacggcg gcgctgctgg atgacatctt gtatgaccgg tccggtggca cgggcaccct cgagtacatg gcgccggagc tcctcgagac aaccgcctct ccacgcggca ccaacgagca gtacgtaaac catcacacaa tggcctccga cgtgtggtcg ctggggatgg tgctgcacta cctcgccttc gatggcacgc tgccggagcg acacgaggac ggcagtgtca acctcgacaa ggcacgccac tcggcgaacg ccagaccccc tgaaatgctc agactgctgg aggccatgtt gcaactcgat cccgcaaagc ggccgcgctg cagcgacatc ctcggctccc caatgatgca gtccatcatg cgcatcttca acaaggatga ccatacccag tgggacctct ccatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat