LemeA.20226.a

Repressor of differentiation kinase 1, putative (RDK1)

CENTER ID: LemeA.20226.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.20226.a.B2.GE42816 PHoranyi kinase domain truncation 1101 1366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.31.0810.1
UniProt: E9B2K5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRNATIPAY IQPSPNHSND HNMSLSLSLQ KQKDANADDE DYADGRRILM HNPLKGHLFS STSDSSLHSS IGQPEMIAAA SSSVQKGSSD PGNRLDTALG LSGEETTQGL VTENVEGPCD EKRVNLIEVH VIDSDDDPSS LEEQEPQVYC TIPSLPAVAF IMCFGAIIVG LLGFLPFYFV GVNSNARTTE YLLSEAMKGV ASITQNSLAM LPAFVHIVTF NYIKRHNTTL EETNLPKDPD QLLVSLLTVL TRFNEAISYL RFVYEGGLYT NAGYTDPKPS DANQTRKIYG GHSRTLNTVP MLEMDNESVT AVEPPNVLGC FDFHREVTLP EGAMNQIVKA WVADPQNSTR WLLASDNTTP TYFNFVMPFI VNGSLGYFEA GSSSDKIIEE TTTLVSFLRK NGRIFLVDAT RNILVGNSWN QSVQSRTDDN AIGATIYTPT RIYEITEPII LAAMQVVNES GSLTEVLKDK DTALITFWYK GNNAFLNLTK VKDSYGLDLV LGVVVVRADF EKAFSTARTA AFTVTVLVVV IAAIVAVVIA WFVVREMKRL IPQLIRASNL EVSRGDGKDH RVQGWLAYIT EIRGIHEAFV RVERSLREMR TFVPAAVMTL APSDDSHSDA DLADGKTRRV RTRFNANIVK DNTNEFSRVD VAMVLVDVHR AMDATEACFI MDIISQRAEE YTGYIESVSS SSFFVNFGTQ SQNPLVVSKI CRFALEVYQS VPDFMRHRVV CFAVRMPFLV GTCGARHSKA RVVFDTQRML GISKVLWDIG CHVASTTDTL SHFATSSAQI PWYRIDCVRF PDEVSQVTLC ELLDPCSSLQ DNENMPKRMG DGLSKMCKGE YKEALMIFDS AHSENVQLKR LRKICIDRIA SGDTSKYIHL IQDVYVLDST PSNHEDMAIG GGAAMYDCDA DNPIGVATNS GEVCDRSNEP PFFACNSFGN GAGMPAPVLS QPAAHASSPV ARHPAPTCNS KTSPTPSVTT GGEAAEPSHL TERAVTAAMG PREFPPCGAL QVAGASGAGP GLPPFHPRTP GATTTPASMP GSSPPCGARD DSASEAPSFL MNAFVFDSSM ASAASADAVG ATVQAADEPQ VLRDVNQHEW QVSLYPIGSG AFSFIYLGMS DDGVQVAIKR IPRLHRGIKE EEMVSEVCTC AKLRHPNIVP YISCCVTQSY LAIIMEYMPG GSLHDIIDNF GKLPRTVVRR FMLDIVNGLA YLHESTTHGD VKPHNILLGV DGVCKLSDFG SASDRLTEAC CVNEDRLMRG TAVYISPEGA RNLPLTSASD IYSLGISFLE MVLGRLPWRW ADPERTDALP LRHDHDFVQC LTANAIAVDI PDDLDNDVRE LALASCAENP EKRPTAQELL SFAFLI
NT Sequence
atgatccgga acgccacgat cccggcgtac atccagccca gcccgaacca cagcaacgac cacaacatga gcctgtcgct gagcctgcag aagcagaagg acgcgaacgc tgatgatgag gactacgccg acggccgacg catcttgatg cacaacccgc tcaagggcca tctcttcagc agcacctcgg actcctcgct tcacagctcc atcggccagc cggagatgat cgcggcggct tccagcagcg tgcagaaggg gagcagcgac cctggcaacc gtctcgacac agcgctcggc ttgtccggag aggagacgac gcagggactg gttacggaga acgtcgaggg cccgtgtgat gagaagcgcg tgaacctgat tgaggtgcac gtcatcgact cggacgacga tccatcctcg cttgaggagc aggagccaca agtttactgc accattccca gccttcccgc cgttgctttt atcatgtgct ttggcgccat cattgtgggg ctgttgggct tccttccctt ctacttcgtc ggcgtcaact ccaacgcgcg cacaacagag tatctgctga gtgaggccat gaagggcgtc gcctctatca cgcagaacag cctggcgatg cttcccgcgt ttgtgcacat tgtgacgttc aactacatca agcgtcataa cacgacactg gaggagacga acttgcccaa ggacccggat cagctgctgg tatcgctcct cacggttctc actcgcttca acgaggccat ctcgtatttg cggttcgtct acgaaggcgg tctctacacg aatgctggct acacagaccc gaagccaagc gatgcgaatc agacccgcaa gatctacggc ggtcacagtc gcacgctcaa cacggtcccc atgctcgaaa tggacaatga gagcgtcacc gctgttgagc cgccaaacgt cttgggttgc ttcgacttcc accgcgaagt caccctccct gaaggtgcca tgaatcagat tgtgaaagcg tgggtggcgg acccgcagaa cagcacgcgc tggctgctgg cctccgataa caccactccg acgtacttca actttgtgat gcctttcatc gtcaacggct ccctcggcta cttcgaggct ggctcctcat cggacaagat catcgaagaa acaaccaccc tcgtaagctt tctgcgcaag aacggtcgca tctttctcgt ggatgccaca cgcaacattc ttgtcggtaa cagctggaac caatcagtgc agagcagaac ggatgacaac gcgatcggcg caaccatcta caccccgact cgcatctacg aaatcaccga gcccatcatt ctcgctgcca tgcaggtggt taacgagagc ggtagcttga cggaggtgtt gaaggacaag gacacagccc tcatcacctt ttggtacaag gggaacaacg cattcctgaa cctaacaaag gtgaaggact cgtacgggct ggacctcgtt cttggcgtcg tggtcgtgcg ggctgacttc gagaaggcct tctccactgc gcgcacggcg gccttcacgg tgacggtctt ggtggttgtg atcgccgcta tcgtcgctgt tgtcatcgcg tggtttgtcg tgcgggagat gaagcggctc atcccgcagc tcatccgcgc gtcaaacctt gaggtgtccc gcggcgacgg caaggatcac cgcgtgcagg gctggctggc gtacattacg gagattcgcg gcattcacga ggccttcgtg cgcgtcgagc ggagtttgcg agagatgcgc acgtttgtgc cggcggccgt catgactctg gcgccgtccg acgactctca cagcgacgcg gatcttgctg acggcaagac gcgccgtgtg cgtacgcgct ttaacgctaa catagtcaag gacaacacga acgagttctc acgagtggat gtggcgatgg tactggtgga cgtgcaccgc gcaatggacg cgactgaggc atgctttatt atggatatca tctcgcagcg cgccgaggag tacaccggct acatcgagtc cgtcagttct agcagcttct tcgtcaattt cggcacccag tcgcagaacc cgctcgtggt gtcgaagatt tgccgcttcg cgctcgaggt gtaccagtcc gtgccggact tcatgcgcca ccgcgtcgtc tgcttcgctg tccgcatgcc gtttcttgtt ggcacgtgcg gcgcacggca cagcaaggcc cgcgtcgtct tcgacacgca gcggatgctg ggcatttcaa aggtgctgtg ggacatcggc tgccacgtcg cctctacgac agacacactc tcgcacttcg ccacgagcag cgcgcagatc ccgtggtacc ggattgactg cgtgcgcttc ccagacgagg tttcgcaggt gacactgtgc gagctgctgg acccgtgctc gtcgctgcag gataatgaaa acatgccaaa gaggatgggc gacggtctct cgaaaatgtg caagggtgag tacaaggagg cgcttatgat ctttgactcg gcgcacagcg agaacgtgca gctgaagcgg ctgcgcaaga tctgcattga ccgcatcgcc tctggcgaca caagcaagta cattcatctc attcaggacg tctacgtgct ggactccacc cccagcaacc acgaagacat ggcgattggc ggcggtgctg cgatgtacga ctgcgacgcc gacaacccca tcggcgtggc gacgaacagc ggcgaggtgt gcgatcgatc caacgagcca ccgttctttg catgcaactc cttcgggaat ggtgcgggga tgccggcacc agtgctgtcg cagcccgcag cacacgcctc gtctccggtt gcgcgtcacc ccgcaccaac gtgcaactcg aagacgtcgc cgacgccgtc cgtgacgact ggcggtgagg cggccgagcc ctcgcacctc accgagcggg ctgtgacggc cgccatgggt ccccgcgagt tccctccatg cggagcgctg caggtggctg gagccagcgg cgcgggtcca ggtctccctc cctttcatcc acgaacccct ggcgctacga cgacgccagc cagcatgcct ggaagctccc cgccctgtgg cgcacgcgac gacagcgcga gtgaggcgcc gtcgtttctc atgaacgcct tcgtcttcga ctcctccatg gcttccgcgg cctccgcgga cgctgttggt gcgaccgtgc aggcggcaga tgagccccag gtgttgcggg acgtgaacca gcacgagtgg caggtctccc tctacccaat cggcagcggc gccttcagct tcatctatct gggcatgtca gacgacggcg tgcaggtggc gatcaagcgc attccccggc tgcaccgcgg catcaaggag gaggagatgg tgtccgaggt atgcacgtgc gccaaactgc gacatcccaa cattgttccc tacatctcct gctgtgtaac ccagtcgtac ctggcaatca tcatggaata catgccaggg ggctcgctgc acgacatcat cgacaacttt ggcaagctgc ctcgcaccgt cgtgcgccgg ttcatgctcg acattgtgaa cggcctagcg tacctgcatg agtccaccac gcacggtgac gtgaagccgc acaacattct gcttggcgtg gacggtgtct gcaagctctc cgactttggc tccgcctcgg acaggctcac ggaggcgtgc tgcgtcaacg aggaccgcct gatgcgcggc acggccgtgt acatctcacc cgagggtgcg cgcaaccttc cactgacctc cgcctctgac atctactcgc tcggtatctc gttcttggag atggtgctgg gccgcctgcc gtggaggtgg gccgatccgg agagaacaga cgcgctcccg ctgcgtcacg accacgattt tgtgcagtgc ctcaccgcca atgccattgc tgtcgacatt cccgacgacc tggacaacga cgtgcgcgag ttggcgctgg cgagctgcgc tgagaaccca gaaaaacgac cgacagccca agagctgctt tccttcgcct tcctgatttg a
Details for LemeA.20226.a.B2.GE42816
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMQ VSLYPIGSGA FSFIYLGMXX DGVQVAIKRI PRLHRGIKEE EMVSEVCTCA KLRHPNIVPY ISCCVTQSYL AIIMEYMPGG SLHDIIDXXG KLPRTVVRRF MLDIVNGLAY LHESTTHGDV KPHSILLGVD GVCKLSDFGS ASDRLTEACC VNEDXXMRGT AVYISPEGAR NLPLTSASDI YSLGISFLEM VLGRLPWRWA DPERTDALPL RHDHDFVQCL TANAIAVDIP DDLDNDVRXX ALASCAENPE KRPTA
Validated NT Sequence
tgggctgtcg gtcgtttttc tgggttctca gcgcagctcg ccagcgccan nncgcgcacg tcgttgtcca ggtcgtcggg aatgtcgaca gcaatggcat tggcggtgag gcactgcaca aaatcgtggt cgtgacgcag cgggagcgcg tctgttctct ccggatcggc ccacctccac ggcaggcggc ccagcaccat ctccaagaac gagataccga gcgagtagat gtcagaggcg gaggtcagtg gaaggttgcg cgcaccctcg ggtgagatgt acacggccgt gccgcgcatc nnnnngtcct cgttgacgca gcacgcctcc gtgagcctgt ccgaggcgga gccaaagtcg gagagcttgc agacaccgtc cacgccaagc agaatgctgt gcggcttcac gtcaccgtgc gtggtggact catgcaggta cgctaggccg ttcacaatgt cgagcatgaa ccggcgcacg acggtgcgag gcagcttgcc aannttgtcg atgatgtcgt gcagcgagcc ccctggcatg tattccatga tgattgccag gtacgactgg gttacacagc aggagatgta gggaacaatg ttgggatgtc gcagtttggc gcacgtgcat acctcggaca ccatctcctc ctccttgatg ccgcggtgca gccggggaat gcgcttgatc gccacctgca cgccgtcgtn nnacatgccc agatagatga agctgaaggc gccgctgccg attgggtaga gggagacctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQV SLYPIGSGAF SFIYLGMSDD GVQVAIKRIP RLHRGIKEEE MVSEVCTCAK LRHPNIVPYI SCCVTQSYLA IIMEYMPGGS LHDIIDNFGK LPRTVVRRFM LDIVNGLAYL HESTTHGDVK PHNILLGVDG VCKLSDFGSA SDRLTEACCV NEDRLMRGTA VYISPEGARN LPLTSASDIY SLGISFLEMV LGRLPWRWAD PERTDALPLR HDHDFVQCLT ANAIAVDIPD DLDNDVRELA LASCAENPEK RPTAQELLSF AFLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaggtct ccctctaccc aatcggcagc ggcgccttca gcttcatcta tctgggcatg tcagacgacg gcgtgcaggt ggcgatcaag cgcattcccc ggctgcaccg cggcatcaag gaggaggaga tggtgtccga ggtatgcacg tgcgccaaac tgcgacatcc caacattgtt ccctacatct cctgctgtgt aacccagtcg tacctggcaa tcatcatgga atacatgcca gggggctcgc tgcacgacat catcgacaac tttggcaagc tgcctcgcac cgtcgtgcgc cggttcatgc tcgacattgt gaacggccta gcgtacctgc atgagtccac cacgcacggt gacgtgaagc cgcacaacat tctgcttggc gtggacggtg tctgcaagct ctccgacttt ggctccgcct cggacaggct cacggaggcg tgctgcgtca acgaggaccg cctgatgcgc ggcacggccg tgtacatctc acccgagggt gcgcgcaacc ttccactgac ctccgcctct gacatctact cgctcggtat ctcgttcttg gagatggtgc tgggccgcct gccgtggagg tgggccgatc cggagagaac agacgcgctc ccgctgcgtc acgaccacga ttttgtgcag tgcctcaccg ccaatgccat tgctgtcgac attcccgacg acctggacaa cgacgtgcgc gagttggcgc tggcgagctg cgctgagaac ccagaaaaac gaccgacagc ccaagagctg ctttccttcg ccttcctgat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat