LemeA.19501.a

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID:	LemeA.19501.a
ORGANISM:	Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemeA.19501.a.B2.GE40701	C-term domain		373	650
LemeA.19501.a.B4.GE41128	N-extended J-binding domain		84	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79392

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LmxM.36.0390
UniProt:	E9ASF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSKRTEAMT ADEEPRRTSA VSNGSSSVSS TNSDEASSSV SDSSSSSGSG SSSGSESSDS ERHSFPPQRQ NSATTERTTS TQQPQQQRHV QLNPELRFER PDKEYIPLPP LRDMQDMAKV LFLLSTDKKR YPDGRHRTLD YFRVAVEMFV TEVRQEYQRQ YQQAQRSGRA MQRFTWKNSG ELAICFACCC DNVKLLYDSL QPGPLKPLWD AFVGQLAPML IVQSRVPETM LSFQTYHTKY MDWVKGGTRY GGEQSTANVR FPSVADRRVK VETYLQSGAG YKIEAVQVGS TSAAAPRPSS AAAAAPVQAL ETRAPFAGRL PGTGSVKVPV APGAGGPRPP VKVPMKHVGG GAAHGSVTAA AAPPLASTSV KQMPPEMKHL FWLSVLAHVK GGDASQAFPD DRLEALPGLL RLCEMTEHTE EMCILMDVLA ASSSGVQTVF ERLGGLLSFR RFASHFIQSE EAEPLMQLVE QLARWRVAAW GNTSQQAWQT DMKQRRTRDL MWLRTLSLIP EGKRAAWGAL LLVMEQRYIF KAPRPDDTTT TDNPLKRRRP EVELGDLHAV LSDNVDMQRW VVPRVNVFSA VRLPVELRVP PLCTKLEEDT VRFLNSKREY AEKAQVTWRE RLEKILSAQP RVPGAAVAVP LWYDPYALLS VASPQP

NT Sequence

atgtcctcaa aacgcaccga ggccatgact gctgacgagg agccgcggcg gacgtcggcc gtgtctaacg gctcgtcgag cgtcagcagt accaactctg acgaagccag cagcagtgtc tccgacagca gcagcagcag cggcagcggg agctcctccg gctccgaatc gtccgatagc gagcgccact cgttccctcc gcagcgccaa aattccgcta caacagagag aaccacgtcc acgcagcagc cacaacagca gcggcatgtg cagctgaatc ccgagctgcg ctttgagcgg ccagacaagg agtacatccc gctgccaccc ctcagagaca tgcaggacat ggccaaggtt ctgtttctct tgagcaccga taagaaacgc tatccggatg ggcgtcaccg cacactggac tacttccgtg tcgcggtgga gatgtttgtg acggaggtgc gccaggagta ccaacggcag tatcagcagg cgcagcgcag cgggcgcgcg atgcagcggt ttacgtggaa gaactcgggc gagctggcga tttgttttgc ctgctgctgc gacaatgtga agcttctgta cgacagcctc caacctggcc ccctcaagcc actctgggac gcctttgtcg gccagctcgc gccgatgctc atcgttcaga gtcgcgtgcc ggaaacgatg ctctcattcc aaacgtacca caccaagtac atggactggg tgaagggcgg cacacgttac ggtggtgagc agtcaaccgc gaatgtccgc ttcccctccg tggcagaccg tcgcgtaaag gttgagacat acctccagag cggcgcgggg tacaagatcg aggctgtgca ggtggggtcg acgtccgccg cggcgcctcg cccttcttcg gctgctgctg cggctccggt gcaggcttta gagactcggg cgcctttcgc ggggcggctg cccggcacag gttcggtgaa ggtgccggtg gctcccggtg ccggtggacc tcgcccacca gtgaaggttc ccatgaagca cgtcggtggc ggtgctgcgc acggcagtgt cacggccgcc gccgctcctc cgcttgcctc cacgagcgtg aagcagatgc cgccggagat gaagcatctg ttttggttgt ccgtgctggc gcacgtaaag ggcggggatg cgtcccaggc gtttccagat gaccgactgg aggcgcttcc cgggctgctg cggctgtgcg agatgacaga gcacacggag gagatgtgca tcttgatgga tgtgctggcg gcctcgagca gcggcgttca gacagtcttc gagcgacttg ggggccttct gtcattccgc cggtttgcct cccacttcat ccagagtgag gaagcagagc cgctcatgca actcgttgag cagctcgcga ggtggcgcgt cgcggcatgg ggcaatacct cacagcaggc gtggcagacc gatatgaaac aacggcgcac acgcgatctg atgtggctcc gaaccctgtc cctcatcccg gagggaaagc gggcggcgtg gggggcgctg ctgctggtga tggagcaacg ctatattttc aaggccccgc gccctgatga cacaaccacc acggacaatc cactgaagcg gcggcgaccc gaggtcgagc tcggggacct gcacgctgtg ctgagtgaca acgtcgatat gcaaagatgg gtggtgcctc gggtcaatgt gttttccgct gtccgcctac cagtggagct gcgcgtgccg ccactgtgca caaagttgga ggaggacact gtgcgcttcc tcaactccaa acgcgagtac gccgagaagg cgcaggtgac gtggcgcgag aggctagaga agattctgag cgcgcagcct cgtgtcccgg gtgccgccgt ggcggtgcct ctctggtatg acccgtacgc gctgctgagc gtggcgtctc cgcagccgtg a

Details for LemeA.19501.a.B2.GE40701

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP PEMKHLFWLS VLAHVKGGDA SQAFPDDRLE ALPGLLRLCE MTEHTEEMCI LMDVLAASSS GVQTVFERLG GLLSFRRFAS HFIQSEEAEP LMQLVEQLAR WRVAAWGNTS QQAWQTDMKQ RRTRDLMWLR TLSLIPEGKR AAWGALLLVM EQRYIFKAPR PDDTTTTDNP LKRRRPEVEL GDLHAVLSDN VDMQRWVVPR VNVFSAVRLP VELRVPPLCT KLEEDTVRFL NSKREYAEKA QVTWRERLEK ILSAQPRVPG AAVAVPLWYD PYALLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc cggagatgaa gcatctgttt tggttgtccg tgctggcgca cgtaaagggc ggggatgcgt cccaggcgtt tccagatgac cgactggagg cgcttcccgg gctgctgcgg ctgtgcgaga tgacagagca cacggaggag atgtgcatct tgatggatgt gctggcggcc tcgagcagcg gcgttcagac agtcttcgag cgacttgggg gccttctgtc attccgccgg tttgcctccc acttcatcca gagtgaggaa gcagagccgc tcatgcaact cgttgagcag ctcgcgaggt ggcgcgtcgc ggcatggggc aatacctcac agcaggcgtg gcagaccgat atgaaacaac ggcgcacacg cgatctgatg tggctccgaa ccctgtccct catcccggag ggaaagcggg cggcgtgggg ggcgctgctg ctggtgatgg agcaacgcta tattttcaag gccccgcgcc ctgatgacac aaccaccacg gacaatccac tgaagcggcg gcgacccgag gtcgagctcg gggacctgca cgctgtgctg agtgacaacg tcgatatgca aagatgggtg gtgcctcggg tcaatgtgtt ttccgctgtc cgcctaccag tggagctgcg cgtgccgcca ctgtgcacaa agttggagga ggacactgtg cgcttcctca actccaaacg cgagtacgcc gagaaggcgc aggtgacgtg gcgcgagagg ctagagaaga ttctgagcgc gcagcctcgt gtcccgggtg ccgccgtggc ggtgcctctc tggtatgacc cgtacgcgct gctgagctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for LemeA.19501.a.B4.GE41128

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHPQ QQRHVQLNPE LRFERPDKEY IPLPPLRDMQ DMAKVLFLLS TDKKRYPDGR HRTLDYFRVA VEMFVTEVRQ EYQRQYQQAQ RSGRAMQRFT WKNSGELAIC FACCCDNVKL LYDSLQPGPL KPLWDAFVGQ LAPMLIVQSR VPETMLSFQT YHTKYMDWVK GGTRYGGEQS TANVRFPSVA DRRVKVETYL QSGAGYKIEA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccacaac agcagcggca tgtgcagctg aatcccgagc tgcgctttga gcggccagac aaggagtaca tcccgctgcc acccctcaga gacatgcagg acatggccaa ggttctgttt ctcttgagca ccgataagaa acgctatccg gatgggcgtc accgcacact ggactacttc cgtgtcgcgg tggagatgtt tgtgacggag gtgcgccagg agtaccaacg gcagtatcag caggcgcagc gcagcgggcg cgcgatgcag cggtttacgt ggaagaactc gggcgagctg gcgatttgtt ttgcctgctg ctgcgacaat gtgaagcttc tgtacgacag cctccaacct ggccccctca agccactctg ggacgccttt gtcggccagc tcgcgccgat gctcatcgtt cagagtcgcg tgccggaaac gatgctctca ttccaaacgt accacaccaa gtacatggac tgggtgaagg gcggcacacg ttacggtggt gagcagtcaa ccgcgaatgt ccgcttcccc tccgtggcag accgtcgcgt aaaggttgag acatacctcc agagcggcgc ggggtacaag atcgaggctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat