LemeA.18709.b

Mitogen-activated protein kinase kinase 3, putative

CENTER ID: LemeA.18709.b
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.18709.b.HZ2.GE43728 kinase domain (Jensen) 22 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.17.0060
UniProt: E9ARC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFYALQDSL LRNSKSGGSS DTVDVESRYV LIEKIGSGSY GDVWSATRRT GAKDVYAVKR IDKRHAGIKG LRSVMGEVET MSLLSHPNIV KLEETYQDEA CLSIVMEYLP GGSLQHRIDR QNVSELETRR FITQLLMAVE YIHEKGIVHR DLKPSNCLLS QNDLVVKISD FGLSVFAGNK QCLTTCCGTL HFMAPEILLE KNYGKPVDMW AMGVMAHVMF FGRYPFQART EAALTKDICR GYLPREEGGG GLPCPSLLQD FISQLLLYDP HRRMSAKDAL KHPWIKEGLD ANRRTSLYPS QAPSVTAMKP SARWRAAVLA VIGVQRLLYL QRIQRLVRLG YDGFPILRDY RYLVTGKYVP ASTSVECSHM FHARPMALLE LISMINTCPF LKHVDLSWNN IHSLSVIQSL LKVVTRHPSL QSIDLSHNPI PAVAGRSIVR LIRNPLSQVT SINVGDTGIS ADTIGQINSF LKEKLTPAAL SYSASTGDMQ SRLTQGSCDD ALMTSSSTTV SSLQQKRSIG SGPLKQPAKK ESRGVRLPPI SRPPAARRHG KMP
NT Sequence
atggacttct acgctttgca agacagtctc ttgcgcaatt ccaagagcgg aggcagctct gacaccgtcg acgtcgagtc taggtacgtc ttgatagaga aaatcggctc tggctcttat ggcgatgtct ggagcgcgac tcggcgcaca ggggcgaagg acgtgtacgc agtgaagcgc atcgacaagc gtcacgcggg catcaaggga ctgcggagcg tcatgggcga agtggagacg atgagccttc tttctcatcc caatatcgtc aagctcgagg agacgtatca agacgaagct tgcctatcaa ttgtgatgga gtacctccct ggcggttccc ttcagcaccg catagaccgg caaaacgtgt ctgaactgga gacgcggcgc ttcatcacgc agctgctgat ggctgtcgag tacattcatg aaaagggtat cgtccaccgc gatttgaagc cgagcaactg cctactatct caaaatgacc tcgtcgtaaa gatatcagac ttcgggctat ccgtgtttgc cggcaataag caatgcttga cgacgtgctg tggcacgctg cacttcatgg cacccgagat tctgctagag aagaactacg gtaagccggt ggacatgtgg gccatgggcg tcatggcaca cgtgatgttt ttcggtcgtt acccatttca ggcccgtaca gaggcggcgc tgacgaagga catctgccgc gggtatcttc cgcgggagga gggtggtggt gggctgccat gtccatcgct gctacaagac tttatcagtc aacttctcct gtacgaccca caccgtcgaa tgagcgccaa agatgctctc aagcacccat ggatcaagga aggtttagac gctaatcggc ggacgtccct ctacccgtca caagcacctt cggtgacagc gatgaagcca tctgcgcgct ggcgggcggc ggtgcttgca gtcataggcg tacagcggct cctctacctg cagagaattc agcggctagt gcggctcggt tatgacgggt ttccaatttt gcgtgactac cgctacctcg taaccggcaa gtatgtacct gcaagcacct ctgtagagtg cagccacatg tttcacgcgc gaccgatggc gctgctcgag ttgatctcca tgataaatac gtgcccgttc ttgaaacatg tagatcttag ctggaataac atccacagcc tatctgtaat ccagtcgctg ctcaaggtgg tgacacgcca cccttcgctg caatctattg acctgtctca caaccccatc ccggccgtgg cagggcgttc aatcgtgcga ctgatccgaa atccgctttc acaagtgaca tccatcaacg tcggcgacac cggaatcagt gctgacacca ttgggcagat caactctttt ttgaaggaga agctgacccc tgccgcgctg tcgtacagcg cctccacagg agacatgcaa agccgactca ctcaaggcag ctgcgatgac gcgctcatga cgtcatcgtc cactacggtg agctccttgc agcagaagcg tagcattggt agtgggccac tcaagcagcc cgcgaagaaa gagtcgcgcg gcgtgcggct ccccccaatt tcacgtccac cagcggctag gcgccatggg aagatgcctt ag
Details for LemeA.18709.b.HZ2.GE43728
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 73
PERCENT COVERAGE: 53
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFXIKK TTPLRRLMEA FAKRQGKEMD SLRFLXXGIX IQADQTPEDL DMEDNDIIEA XXEQIGGTLE XXTXGPGSMT VXVXSRYXLI XKIGSGSYGX VWSATXXTGA KDVYAVKRID XRHAGIKGTA ERXGRSGDDX PSFSSQYRQA RXRRIXDEAC LSIVMEYLPG XFPSAPHRPA XRXLNWRRGA SSRSC
Validated NT Sequence
gnaaacccgt cataaccgag ccgcactagc cgctgaattc tctgcaggta gaggagccgc tgtacgccta tgactgcaag caccgccgcc cgccagcgcg cagatggctt catcgctgtc accgaaggtg cttgtgacgg gtagagggac gtccgccgat tagcgtctaa accttccttg atccatgggt gcttgagagc atctttggcg ctcattcgac ggtgtgggtc gtacaggaga agttgactga taaagtcttg tagcagcgat ggacatggca gcccaccacc accctcctcc cgcggaagat acccgcggca gatgtccttc gtcagcgccg cctctgtacg ggcctgaaat gnnnanngac cgaaaaacat cacgtgtgcc atgacgccca tggcccacat gtccaccggn ttaccgtagt tcttcnnnnn nanaatctcg ggtgccatga anngnnncgt gccacagcac nncnncaagc attgcttatt gccggcaaac acggatagcc cgaagtctga tatctttacg acgaggtcat tttgagatag taggcagttg ctcggcttca aatcgcggtg gacgannncc ttttcatgaa tgtactcgac agccatcagc agctgcgnga tgaagcgccg cgtctccagt tcagnncncg tnttgccggt ctatgcggng ctgangggaa ccnnccaggg aggtactcca tcacaattga taggcaagct tcgtcntnga tacgtctccn tcgagcttga cgatattggg atgagaaaga aggntcatcg tctccacttc gcccntgacg ctccgcagtc cccttgatgc ccgcgtgacg ctngtcgatg cgcttcactg cgtacacgtc cttcgcccct gtgcnnngag tcgcgctcca gacntcgcca taagagccag agccgatttt ntctatcaag angtacctag actngacgtn gacggtcatn gaaccaggac cntgggtntg agnttccagg gtaccaccaa tctgttctnn nnnagcctca ataatatcgt tatcctccat gtccaaatct tcaggggtct gatcagcttg aattntaata ccgtngnnca agaatcttaa ggagtccatt tccttnccct gtcttttagc gaacgcttcc atcagccttc ttaaaggagt ggtctttttg atcnngaaga agatctctga agatccatcg gacaccttta aattgatgtg agtctcaggc ttgacttctg gcttgacctc tggcttagct tcttgattga cttctgagtc cgacttcata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSTV DVESRYVLIE KIGSGSYGDV WSATRRTGAK DVYAVKRIDK RHAGIKGLRS VMGEVETMSL LSHPNIVKLE ETYQDEACLS IVMEYLPGGS LQHRIDRQNV SELETRRFIT QLLMAVEYIH EKGIVHRDLK PSNCLLSQND LVVKISDFGL SVFAGNKQCL TTCCGTLHFM APEILLEKNY GKPVDMWAMG VMAHVMFFGR YPFQARTEAA LTKDICRGYL PREEGGGGLP CPSLLQDFIS QLLLYDPHRR MSAKDALKHP WIKEGLDANR RTSLYPSQAP SVTAMKPSAR WRAAVLAVIG VQRLLYLQRI QRLVRLGYDG FP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga ccgtcgacgt cgagtctagg tacgtcttga tagagaaaat cggctctggc tcttatggcg atgtctggag cgcgactcgg cgcacagggg cgaaggacgt gtacgcagtg aagcgcatcg acaagcgtca cgcgggcatc aagggactgc ggagcgtcat gggcgaagtg gagacgatga gccttctttc tcatcccaat atcgtcaagc tcgaggagac gtatcaagac gaagcttgcc tatcaattgt gatggagtac ctccctggcg gttcccttca gcaccgcata gaccggcaaa acgtgtctga actggagacg cggcgcttca tcacgcagct gctgatggct gtcgagtaca ttcatgaaaa gggtatcgtc caccgcgatt tgaagccgag caactgccta ctatctcaaa atgacctcgt cgtaaagata tcagacttcg ggctatccgt gtttgccggc aataagcaat gcttgacgac gtgctgtggc acgctgcact tcatggcacc cgagattctg ctagagaaga actacggtaa gccggtggac atgtgggcca tgggcgtcat ggcacacgtg atgtttttcg gtcgttaccc atttcaggcc cgtacagagg cggcgctgac gaaggacatc tgccgcgggt atcttccgcg ggaggagggt ggtggtgggc tgccatgtcc atcgctgcta caagacttta tcagtcaact tctcctgtac gacccacacc gtcgaatgag cgccaaagat gctctcaagc acccatggat caaggaaggt ttagacgcta atcggcggac gtccctctac ccgtcacaag caccttcggt gacagcgatg aagccatctg cgcgctggcg ggcggcggtg cttgcagtca taggcgtaca gcggctcctc tacctgcaga gaattcagcg gctagtgcgg ctcggttatg acgggtttcc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa