LemeA.10252.a

threonyl-tRNA synthetase, putative

CENTER ID: LemeA.10252.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.10252.a.B2.GE43801 TEdwards Stma structure domain 284 787
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.10252.a.B2.PW38952 TEdwards Stma structure domain 284 787

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.34.1410
UniProt: E9B620

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGKKATEAT VNLATLAEPD FWRQRVEIFE KLWQQQQNRY ESMKAPIKVS LPDGKVMDAE SWVTCPRDIA RRLSNSLPDK VIVARVNEAL WDLTRPFEAD CQLELLDWDD KDAREVFWHS SSHVLGYALE RIFDTKLSVG PALEEGGFFY EGLTNRPVSE SDYKAIESAM QELVKQKMPY QRLEVSKEDA LRLFGYTEFK SKILASKVPD GGSCTVYRCG MLIDPCRGPH LPDTGRVKAF AVTKNSSSYF EGKAENEVLQ RVYGISFPKN PMLTEWKMIQ EEAAKRDHRV IGRQQNLFNF HEVSPGSAFW LPHGARIYNT LVEFLRKKYR CCGFQEVISP NMYSSKLWMV SGHWEKYADN MFCTKCEKED FGLKPMNCPG HCIMFASQPH SYKELPIRYA DFGVLHRNEL SGALTGLTRV RRFQQDDAHI FCRMDQITDE MEGQMQFLSD VYGVLGFKFY FYHSTRPANK LGSDALWDQS ESYLRTALNR FCGIPEELPD PLHPDQRFHY DGKPDSVKKM KMLMKKAERS EDPNAWKGPT NGSEGWIENA GDGAFYGPKI DIVVEDALRR RHQCATIQLD FNLPSRFGLK YTLPTAEKDE ATVGPTEKRR HADPASTASS PAVAEGESKP KPGTYEAAVR DLGIDLELDA NQARPVMIHR AILGSLERSI AILCEHFGGD WPFWLSPRQV MVVPVSASNY EYSQQVRDTM HDAGFHADVD NGAATLDKKI RNAEKARYNF ILVVGQQEQD ATGVNIRARG ERRLGNKSLE EALQWFKELA DTHSREY
NT Sequence
atgtctggca agaaggcgac ggaggcgacc gtgaacttgg ccaccctggc ggagccagac ttctggcggc aacgcgtcga aatcttcgag aagctatggc agcagcagca gaaccgctat gagtcgatga aggccccgat caaggtgagc ctaccggatg ggaaggtgat ggacgccgag agctgggtta cctgcccgcg ggacatcgca aggcgcctgt ccaactcgct gccagacaag gtcattgtgg cccgcgtgaa cgaggccctg tgggacttga cgcggccgtt cgaggcggac tgccaactgg agctgctgga ctgggacgac aaggatgcac gtgaggtgtt ctggcacagc tcctcgcacg tgctcggcta cgcgctcgag cgcatcttcg acacgaagct ctccgtcggc ccggcgctgg aggagggcgg cttcttttac gagggcctca ctaatcgccc ggtgtcggag tccgactaca aggcgatcga gtcagccatg caggagctgg tgaagcagaa gatgccatac cagcgtctcg aagtgtcgaa ggaggacgcc ctgaggctct tcggctacac ggagttcaag agcaagatcc tggcaagcaa ggtgccggac gggggctcgt gcaccgtgta ccggtgcggt atgctgattg acccgtgccg tggtccccac ctcccggaca cgggccgcgt caaggccttt gctgtgacca agaactcttc ctcttacttt gagggcaagg ccgagaacga ggtgctgcag cgcgtgtacg gcatttcgtt tccgaagaac cccatgctga cggagtggaa gatgatccag gaggaggctg cgaagcgtga ccaccgcgtg attggccgcc aacagaacct cttcaacttc cacgaggtct cgcctggtag cgccttctgg ctgccgcacg gcgctcgcat ctacaacacc ctcgtagagt tcctgcgcaa gaagtatcgc tgctgcggct tccaggaggt catctcgccg aacatgtact cctccaaact ttggatggtg tcgggccact gggagaagta cgccgacaac atgttctgca ccaagtgcga aaaggaggac tttggtctga agccgatgaa ctgcccgggc cactgcatca tgttcgcgtc gcagccgcac tcgtacaagg agctgccgat tcgctacgcc gactttggcg tgctgcaccg gaacgagctg agcggcgcct tgacggggct gacccgcgtg cgccgcttcc agcaggacga tgcacacatc ttctgccgca tggaccagat cacagatgag atggagggcc agatgcagtt cctcagtgac gtttacggcg tgctgggctt caagttctat ttttaccact ccacccgccc cgcgaacaag ctcggctccg acgcgctctg ggaccagtcc gagtcgtatc tgcgcaccgc gctcaaccgg ttctgcggca ttccggagga actgcccgat ccgctgcacc cggaccagcg ctttcactac gacggcaagc cggactcggt caagaagatg aagatgctca tgaagaaggc ggagcgcagc gaggacccga acgcgtggaa gggcccaacc aacggcagcg agggctggat cgagaacgct ggcgatggcg ccttctacgg ccccaagatc gacatcgtgg tcgaggacgc gctgcgccgc cgccaccagt gcgccacgat ccagttggac ttcaacctgc cgagtcgctt tggcctgaag tacacccttc ccaccgcaga gaaggacgag gcgacggtgg gcccaacgga gaagcggagg catgccgacc ctgcctcgac cgcgtcgtcg ccggccgtgg cagagggcga gagcaaaccg aagcccggca cgtacgaggc tgctgtgcgt gaccttggca tcgacctcga gctggacgcc aaccaggccc gccccgtcat gatccaccgc gccattctcg gttccttgga gcgctcgatc gccattctgt gcgagcactt tggcggcgac tggccgttct ggctgagccc ccgccaagtg atggtggtgc ctgtttcggc ctccaactac gagtattcac agcaggtgcg cgacacgatg cacgatgccg gcttccacgc ggacgtcgac aacggtgccg ccacgctgga caagaagatc cgcaacgcgg agaaggcgcg ctacaacttc atccttgtcg tgggtcagca ggagcaggac gcgaccggcg tcaacatccg cgcgcgcggg gagaggagac tgggtaacaa gtcactcgag gaggcgttgc agtggttcaa ggagctggcg gacacacata gccgggagta ctag
Details for LemeA.10252.a.B2.GE43801
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 27
PERCENT COVERAGE: 28
Validated AA Sequence
MAIERSKEPR MARWIMTGRA WLASSSRSMP RSRTAASYVP GFGLLSPSAT AGDDAVEAGS ACLRFSVGPT VASSFSAVGR VYFXXKRLGX XSPTGSWRTG GGGAARPRPR CRSWGRRXRH RQRSRSSPRX RWLXPSTRSG PRCAPPSS
Validated NT Sequence
ctnttgtnnn cagccggatc ctatcttact cacttagtac tcccggctat gtgtgtccnn nagctccttg aaccactgca acgcctcctc gagtgacttg ttacccagtc tcctctcccc gcgcgcgcgg atgttgacgc cggtcgcgtc ctgctcctgc tgacccacga caaggatgaa gttgtagcgc gccttctccg cgttgcggat cttcttgtcc agcgtggcgg caccgttgtc gacgtccgcg tggaagccgg catcgtgcat cgtgtcgcgc acctgctgtg aatactcgta gttggaggcc gaaacaggca ccaccatcac ttggcggggg ctcagccaga acggccagtc gccgccaaag tgctcgcaca gaatggcgat cgagcgctcc aaggaaccga gaatggcgcg gtggatcatg acggggcggg cctggttggc gtccagctcg aggtcgatgc caaggtcacg cacagcagcc tcgtacgtgc cgggcttcgg tttgctctcg ccctctgcca cggccggcga cgacgcggtc gaggcagggt cggcatgcct ccgcttctcc gttgggccca ccgtcgcctc gtccttctct gcggtgggaa gggtgtactt cangncaaag cgactcggca gnnnaagtcc aactggatcg tggcgcactg gtggcggcgg cgcagcgcgt cctcgaccac gatgtcgatc ttggggccgt agaangcgcc atcgccagcg ttctcgatcc agccctcgct gncgttggtt ggncccttcc acgcgttcgg gtcctcgctg cgctccgcct tcttcatgag catcttcatc ttcttgaccg annccggctt gccgtcgtaa nngaaagcnc tgnncgggnn cagcgnncng gcannnntcc nggaatgccg cagaacccgg ttgagcgcgg ngnnnnnann cgactcgnnn tgnnccnnan cgntcnnanc cnancttgtt ncnngnnnng nnnnnnaaaa nnnaacttga accnnnnnnn nnnnaacgtn nctgannntg nnnnnnnnnn ccntnnccnt tggnnnngnc nnnnnnnnna angngnnnnn nnnnnntnnn nanggcnnnn nnnnncnnnc
Expected Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGA RIYNTLVEFL RKKYRCCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSDA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKMLMK KAERSEDPNA WKGPTNGSEG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPT AEKDEATVGP TEKRRHADPA STASSPAVAE GESKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QQEQDATGVN IRARGERRLG NKSLEEALQW FKELADTHSR EY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgaagc gtgaccaccg cgtgattggc cgccaacaga acctcttcaa cttccacgag gtctcgcctg gtagcgcctt ctggctgccg cacggcgctc gcatctacaa caccctcgta gagttcctgc gcaagaagta tcgctgctgc ggcttccagg aggtcatctc gccgaacatg tactcctcca aactttggat ggtgtcgggc cactgggaga agtacgccga caacatgttc tgcaccaagt gcgaaaagga ggactttggt ctgaagccga tgaactgccc gggccactgc atcatgttcg cgtcgcagcc gcactcgtac aaggagctgc cgattcgcta cgccgacttt ggcgtgctgc accggaacga gctgagcggc gccttgacgg ggctgacccg cgtgcgccgc ttccagcagg acgatgcaca catcttctgc cgcatggacc agatcacaga tgagatggag ggccagatgc agttcctcag tgacgtttac ggcgtgctgg gcttcaagtt ctatttttac cactccaccc gccccgcgaa caagctcggc tccgacgcgc tctgggacca gtccgagtcg tatctgcgca ccgcgctcaa ccggttctgc ggcattccgg aggaactgcc cgatccgctg cacccggacc agcgctttca ctacgacggc aagccggact cggtcaagaa gatgaagatg ctcatgaaga aggcggagcg cagcgaggac ccgaacgcgt ggaagggccc aaccaacggc agcgagggct ggatcgagaa cgctggcgat ggcgccttct acggccccaa gatcgacatc gtggtcgagg acgcgctgcg ccgccgccac cagtgcgcca cgatccagtt ggacttcaac ctgccgagtc gctttggcct gaagtacacc cttcccaccg cagagaagga cgaggcgacg gtgggcccaa cggagaagcg gaggcatgcc gaccctgcct cgaccgcgtc gtcgccggcc gtggcagagg gcgagagcaa accgaagccc ggcacgtacg aggctgctgt gcgtgacctt ggcatcgacc tcgagctgga cgccaaccag gcccgccccg tcatgatcca ccgcgccatt ctcggttcct tggagcgctc gatcgccatt ctgtgcgagc actttggcgg cgactggccg ttctggctga gcccccgcca agtgatggtg gtgcctgttt cggcctccaa ctacgagtat tcacagcagg tgcgcgacac gatgcacgat gccggcttcc acgcggacgt cgacaacggt gccgccacgc tggacaagaa gatccgcaac gcggagaagg cgcgctacaa cttcatcctt gtcgtgggtc agcaggagca ggacgcgacc ggcgtcaaca tccgcgcgcg cggggagagg agactgggta acaagtcact cgaggaggcg ttgcagtggt tcaaggagct ggcggacaca catagccggg agtactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LemeA.10252.a.B2.PW38952
PURIFICATION DATe: 3/3/2021
CONCENTRATION: 6mg/ml
OBSERVED MW: 58kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 24
VIAL VOLUME: 32µl
PERCENT IDENTITY: 27
PERCENT COVERAGE: 28
Protocol Notes
difficult to concentrate
Validated AA Sequence
MAIERSKEPR MARWIMTGRA WLASSSRSMP RSRTAASYVP GFGLLSPSAT AGDDAVEAGS ACLRFSVGPT VASSFSAVGR VYFXXKRLGX XSPTGSWRTG GGGAARPRPR CRSWGRRXRH RQRSRSSPRX RWLXPSTRSG PRCAPPSS
Validated NT Sequence
ctnttgtnnn cagccggatc ctatcttact cacttagtac tcccggctat gtgtgtccnn nagctccttg aaccactgca acgcctcctc gagtgacttg ttacccagtc tcctctcccc gcgcgcgcgg atgttgacgc cggtcgcgtc ctgctcctgc tgacccacga caaggatgaa gttgtagcgc gccttctccg cgttgcggat cttcttgtcc agcgtggcgg caccgttgtc gacgtccgcg tggaagccgg catcgtgcat cgtgtcgcgc acctgctgtg aatactcgta gttggaggcc gaaacaggca ccaccatcac ttggcggggg ctcagccaga acggccagtc gccgccaaag tgctcgcaca gaatggcgat cgagcgctcc aaggaaccga gaatggcgcg gtggatcatg acggggcggg cctggttggc gtccagctcg aggtcgatgc caaggtcacg cacagcagcc tcgtacgtgc cgggcttcgg tttgctctcg ccctctgcca cggccggcga cgacgcggtc gaggcagggt cggcatgcct ccgcttctcc gttgggccca ccgtcgcctc gtccttctct gcggtgggaa gggtgtactt cangncaaag cgactcggca gnnnaagtcc aactggatcg tggcgcactg gtggcggcgg cgcagcgcgt cctcgaccac gatgtcgatc ttggggccgt agaangcgcc atcgccagcg ttctcgatcc agccctcgct gncgttggtt ggncccttcc acgcgttcgg gtcctcgctg cgctccgcct tcttcatgag catcttcatc ttcttgaccg annccggctt gccgtcgtaa nngaaagcnc tgnncgggnn cagcgnncng gcannnntcc nggaatgccg cagaacccgg ttgagcgcgg ngnnnnnann cgactcgnnn tgnnccnnan cgntcnnanc cnancttgtt ncnngnnnng nnnnnnaaaa nnnaacttga accnnnnnnn nnnnaacgtn nctgannntg nnnnnnnnnn ccntnnccnt tggnnnngnc nnnnnnnnna angngnnnnn nnnnnntnnn nanggcnnnn nnnnncnnnc
Expressed Protein Sequence
MAHHHHHHAK RDHRVIGRQQ NLFNFHEVSP GSAFWLPHGA RIYNTLVEFL RKKYRCCGFQ EVISPNMYSS KLWMVSGHWE KYADNMFCTK CEKEDFGLKP MNCPGHCIMF ASQPHSYKEL PIRYADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRMD QITDEMEGQM QFLSDVYGVL GFKFYFYHST RPANKLGSDA LWDQSESYLR TALNRFCGIP EELPDPLHPD QRFHYDGKPD SVKKMKMLMK KAERSEDPNA WKGPTNGSEG WIENAGDGAF YGPKIDIVVE DALRRRHQCA TIQLDFNLPS RFGLKYTLPT AEKDEATVGP TEKRRHADPA STASSPAVAE GESKPKPGTY EAAVRDLGID LELDANQARP VMIHRAILGS LERSIAILCE HFGGDWPFWL SPRQVMVVPV SASNYEYSQQ VRDTMHDAGF HADVDNGAAT LDKKIRNAEK ARYNFILVVG QQEQDATGVN IRARGERRLG NKSLEEALQW FKELADTHSR EY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCGAAGC GTGACCACCG CGTGATTGGC CGCCAACAGA ACCTCTTCAA CTTCCACGAG GTCTCGCCTG GTAGCGCCTT CTGGCTGCCG CACGGCGCTC GCATCTACAA CACCCTCGTA GAGTTCCTGC GCAAGAAGTA TCGCTGCTGC GGCTTCCAGG AGGTCATCTC GCCGAACATG TACTCCTCCA AACTTTGGAT GGTGTCGGGC CACTGGGAGA AGTACGCCGA CAACATGTTC TGCACCAAGT GCGAAAAGGA GGACTTTGGT CTGAAGCCGA TGAACTGCCC GGGCCACTGC ATCATGTTCG CGTCGCAGCC GCACTCGTAC AAGGAGCTGC CGATTCGCTA CGCCGACTTT GGCGTGCTGC ACCGGAACGA GCTGAGCGGC GCCTTGACGG GGCTGACCCG CGTGCGCCGC TTCCAGCAGG ACGATGCACA CATCTTCTGC CGCATGGACC AGATCACAGA TGAGATGGAG GGCCAGATGC AGTTCCTCAG TGACGTTTAC GGCGTGCTGG GCTTCAAGTT CTATTTTTAC CACTCCACCC GCCCCGCGAA CAAGCTCGGC TCCGACGCGC TCTGGGACCA GTCCGAGTCG TATCTGCGCA CCGCGCTCAA CCGGTTCTGC GGCATTCCGG AGGAACTGCC CGATCCGCTG CACCCGGACC AGCGCTTTCA CTACGACGGC AAGCCGGACT CGGTCAAGAA GATGAAGATG CTCATGAAGA AGGCGGAGCG CAGCGAGGAC CCGAACGCGT GGAAGGGCCC AACCAACGGC AGCGAGGGCT GGATCGAGAA CGCTGGCGAT GGCGCCTTCT ACGGCCCCAA GATCGACATC GTGGTCGAGG ACGCGCTGCG CCGCCGCCAC CAGTGCGCCA CGATCCAGTT GGACTTCAAC CTGCCGAGTC GCTTTGGCCT GAAGTACACC CTTCCCACCG CAGAGAAGGA CGAGGCGACG GTGGGCCCAA CGGAGAAGCG GAGGCATGCC GACCCTGCCT CGACCGCGTC GTCGCCGGCC GTGGCAGAGG GCGAGAGCAA ACCGAAGCCC GGCACGTACG AGGCTGCTGT GCGTGACCTT GGCATCGACC TCGAGCTGGA CGCCAACCAG GCCCGCCCCG TCATGATCCA CCGCGCCATT CTCGGTTCCT TGGAGCGCTC GATCGCCATT CTGTGCGAGC ACTTTGGCGG CGACTGGCCG TTCTGGCTGA GCCCCCGCCA AGTGATGGTG GTGCCTGTTT CGGCCTCCAA CTACGAGTAT TCACAGCAGG TGCGCGACAC GATGCACGAT GCCGGCTTCC ACGCGGACGT CGACAACGGT GCCGCCACGC TGGACAAGAA GATCCGCAAC GCGGAGAAGG CGCGCTACAA CTTCATCCTT GTCGTGGGTC AGCAGGAGCA GGACGCGACC GGCGTCAACA TCCGCGCGCG CGGGGAGAGG AGACTGGGTA ACAAGTCACT CGAGGAGGCG TTGCAGTGGT TCAAGGAGCT GGCGGACACA CATAGCCGGG AGTACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT