LemeA.01593.a

Protein kinase domain-containing protein

CENTER ID: LemeA.01593.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.01593.a.HZ2.GE43727 kinase domain (Jensen) 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.27.0100
UniProt: E9AYR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKYEILAQI GDGTFGSVAK AVSKKTGQLV AIKKMKQKFY TWEECVKLPE VDVVRRIHGH PNVVKLREVI RENNELFFVF EYMDCDLLGV IKKAKQQGGR PASTPSTAPL IPYPLVKNYM RQMLQALVYI HKRGYFHRDM KPENLLIRKE ASGDEVLKLA DFGLVKEIRA RPPFTDYVST RWYRAPELLL QDRFYGAAVD VWAAGCIMAE LITMRPLFPG TNEVDQLFKI MSVLGSPTEE VWAGGLRLAK KIRYVFPKVA GSGLAQALPS HIPLPALDLL RQMLVYDPKV RLTAEQCLQH PFFNVGIDEC NAPSAAALDQ LAVMAKRMLP GSKTAPPALK SPTMDQVAAL KAARASTDAS LSDTSSRKFY LLGPLKDPSL SLPKLPVALS TSPASLDTLP NISSPLRGRG ALSPAGIPVL ANGHQGPSLT TTLAKGKRRS QETADNLLAK KLGALKNVPT GKGKLPVSYA LPAASAVHSP RAAPNLKVSA SVARPYPLAP TANNRDAVAS PRRHNPYKEA KTTSPTAASA AAKVEIDLDE LMEEFASEMT SMGVLAQRHG SGASAELKSE SYVSQPALPP DPVATLLNNS RYRRKSATSA DPPLKRNNSP RTTHQLDARK VDDAASKSDL PRIDSSAPKG MSPSLKALLA KHKTSGLAFP
NT Sequence
atggacaaat atgaaattct cgctcaaatc ggagatggca cgttcggctc cgtcgcaaag gccgtgagca agaaaaccgg ccagcttgta gccatcaaga agatgaagca gaaattctac acctgggagg agtgcgtcaa gcttcccgag gtggacgttg tgcggcgcat tcacggccac cccaacgtgg tgaagctgcg tgaggtaatt cgagagaaca acgagctgtt cttcgtcttc gagtacatgg actgcgattt gctcggcgtc atcaaaaagg ccaagcagca gggcgggcgg ccagctagta caccctctac tgcaccgttg attccgtacc ctcttgtaaa gaactacatg cgccaaatgc tgcaggcact cgtgtacatc cacaaacgcg gctacttcca ccgagacatg aagccggaaa accttctcat tcggaaagaa gcgagcggcg acgaggtgct gaagctggca gacttcggac tcgtcaagga gatccgtgct cgccccccgt tcaccgacta cgtgtcgacg cggtggtacc gcgcaccaga gctgctcctg caggatcgct tctacggcgc tgcggtggat gtgtgggctg ccgggtgtat tatggcggaa ctcatcacca tgcggccact ttttccaggc acaaacgagg tagatcagct cttcaagata atgtcggtgc ttgggtcccc cactgaggag gtgtgggccg gtggacttcg cttggccaaa aaaatccgct acgtctttcc taaagtagcc ggctccggcc ttgctcaagc gctgccgtcg cacatcccgt tgccggcgct ggacttgctg cgtcagatgc tcgtctacga cccgaaagtg agactgacgg cggagcaatg cctgcagcac ccgttcttca acgtcggcat tgacgagtgc aacgctccat ccgctgccgc tctggaccag ctggcggtca tggcaaagcg gatgctgcca gggtcgaaaa cagcaccgcc cgcgctgaag agccccacca tggaccaggt ggcggcgcta aaggcggcta gggcctccac cgacgcatcg ctctcggaca cctcgtcacg caagttctac ctgctcggcc ccctaaagga cccttccctg tcgttgccaa agctccctgt tgcgttgtcc acctcgccag cgtcccttga cacgctgccg aacatttcgt cacctctgcg tgggcgcggg gctctctcac cagcggggat accagtgctc gccaatggcc accagggccc cagcctgacg acgacactgg ctaagggaaa gcgccgctcg caggaaacag cagacaactt gcttgcgaag aagctaggag ccttgaaaaa cgtcccgacg ggaaaaggga aactgccggt gtcttacgca ctgccggctg ccagcgctgt gcactcacct agagcggcac ccaacctgaa ggtctccgca tccgtggcaa ggccataccc cctggcgccc accgccaaca atcgcgacgc cgtggcgtcc ccgcggcgcc ataacccata caaggaagcc aagactactt ctccgacggc ggcgtccgcg gcggcgaagg tagagattga cttggacgag ctcatggagg agtttgcgtc ggagatgact tccatggggg tgctggcgca gcgacacggc tcgggggcct cggcggaatt gaaaagcgaa agctacgttt cccagccagc gcttccgccc gatcctgtgg cgacgctgct gaacaacagc cggtatagaa ggaaatcggc cacgagcgcc gacccaccgt tgaagaggaa caactcgcct cgcaccacac accagctgga tgcaaggaaa gtggacgatg ctgcctcaaa gagtgacctt cctcgaatag actcctcggc cccgaaagga atgtcaccat cgctgaaggc gctgctagca aagcacaaga cgagtggtct ggcgttcccg tag
Details for LemeA.01593.a.HZ2.GE43727
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 40
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD KYEILXXIGD GTFGSVAKXX SKKTGQLVXX XKMKXKFYTW XXXVXXSRXG RCAAHSRXTP TW
Validated NT Sequence
gggcggtgct gttttcgacc ctggcagcat ccgctttgcc atgaccgcca gctggtccag agcggcagcg gatggagcgt tgcactcgtc aatgccgacg ttgaagaacg ggtgctgcag gcattgctcc gccgtcagtc tcactttcgg gtcgtagacg agcatctgac gcagcaagtc cagcgccggc aacgggatgt gcgacggcag cgcttgagca aggccggagc cggctacttt aggaaagacg tagcggattt ttttggccaa gcgaagtcca ccggcccaca cctcctcagt gggggaccca agcaccgaca ttatcttgaa gagctgatct acctcgtttg tgcctggaaa aagtggccgc atggtgatga gttccgccat aatacacccg gcagcccaca catccaccgc agcgccgtag aagcgatcct gcaggagcag ctctggtgcg cggtaccacc gcgtcgacac gtagtcggtg aacggggggc gagcacggat ctccttgacg agtccgaagt ctgccagctt cagcacctcg tcgccgctcg cttctttccn aatgagaagg ttttccggct tcatgtctcg gtggaagtag ccgcgtttgt ggangtacac gagtgcctgc agcatttggc gcatgtagtt ctttacaaga gggtacggaa tcaacggtgc agtagagggt gtactagctg gccgcccgcc ctgctgcttg gnctttttga tgacgccgag caaatcgcag tccatgtact cgaagacgaa gaacagctcg ttgttctctc gaattncctc acgcagcttc accacgttgg ggtggnncgt gaatgcgccg cacaacgtcc acntcgggaa gnntngacnc antcntccca ggtgtagaat ttntgcttca tcttnnngnn nnntacaagc tgnccggttt tcttgctcnc gncctttgcg acggagccga acgtgccatc tccgatntga gngagaattt catatttgtc catcgaacca ggaccctggg tctgagcttc cagggtacca ccaatctgtt ctctgtgagc ctcaataata tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg agtccgactt catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD KYEILAQIGD GTFGSVAKAV SKKTGQLVAI KKMKQKFYTW EECVKLPEVD VVRRIHGHPN VVKLREVIRE NNELFFVFEY MDCDLLGVIK KAKQQGGRPA STPSTAPLIP YPLVKNYMRQ MLQALVYIHK RGYFHRDMKP ENLLIRKEAS GDEVLKLADF GLVKEIRARP PFTDYVSTRW YRAPELLLQD RFYGAAVDVW AAGCIMAELI TMRPLFPGTN EVDQLFKIMS VLGSPTEEVW AGGLRLAKKI RYVFPKVAGS GLAQALPSHI PLPALDLLRQ MLVYDPKVRL TAEQCLQHPF FNVGIDECNA PSAAALDQLA VMAKRMLPGS KTAPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggacaaata tgaaattctc gctcaaatcg gagatggcac gttcggctcc gtcgcaaagg ccgtgagcaa gaaaaccggc cagcttgtag ccatcaagaa gatgaagcag aaattctaca cctgggagga gtgcgtcaag cttcccgagg tggacgttgt gcggcgcatt cacggccacc ccaacgtggt gaagctgcgt gaggtaattc gagagaacaa cgagctgttc ttcgtcttcg agtacatgga ctgcgatttg ctcggcgtca tcaaaaaggc caagcagcag ggcgggcggc cagctagtac accctctact gcaccgttga ttccgtaccc tcttgtaaag aactacatgc gccaaatgct gcaggcactc gtgtacatcc acaaacgcgg ctacttccac cgagacatga agccggaaaa ccttctcatt cggaaagaag cgagcggcga cgaggtgctg aagctggcag acttcggact cgtcaaggag atccgtgctc gccccccgtt caccgactac gtgtcgacgc ggtggtaccg cgcaccagag ctgctcctgc aggatcgctt ctacggcgct gcggtggatg tgtgggctgc cgggtgtatt atggcggaac tcatcaccat gcggccactt tttccaggca caaacgaggt agatcagctc ttcaagataa tgtcggtgct tgggtccccc actgaggagg tgtgggccgg tggacttcgc ttggccaaaa aaatccgcta cgtctttcct aaagtagccg gctccggcct tgctcaagcg ctgccgtcgc acatcccgtt gccggcgctg gacttgctgc gtcagatgct cgtctacgac ccgaaagtga gactgacggc ggagcaatgc ctgcagcacc cgttcttcaa cgtcggcatt gacgagtgca acgctccatc cgctgccgct ctggaccagc tggcggtcat ggcaaagcgg atgctgccag ggtcgaaaac agcaccgccc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa