LemeA.01391.a

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID: LemeA.01391.a
ORGANISM: Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE: Leishmaniasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LemeA.01391.a.HZ3.GE43725 kinase domain #2 (Jensen) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LmxM.36.0720
UniProt: E9ASJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAEIESHIL KKYEIQTQLG QGAYGIVWRA LERKHNRVVA LKKIYDAFQN STDAQRTFRE IMFLHRLHHP NIIRLLHVHR AFNDRDIYLV FEYMETDLHV VIRANILEGI HKQFIIYQLL KTMKFLHSAE ILHRDMKPSN LLVNSDCTMK VADFGLARSI LSLEGEQASR PVLTDYIATR WYRPPEILLG STRYTKGVDM WSVGCILGEL MLGKPMFPGR STTNQLELIC SVTGMPSAAD VAATNSQFAN AMLRDIHCAH RRTFAELLPS ASADALNLIE RLMCFNPNRR LSAAEALEHP YVAAFHRPDD EPVAPEPITV SLPDSQRLPL AKYRDAIYEQ IASLRRISTS TDHRHRAERH GAGGTASRKT SASGGGGAAG GSRGGTGTSG ATRPATSCSS STAARAAPQR SVVKPTSTSG VNESSSSKAY GRPGFRSVAS TSSGLESRPV EREAAVRK
NT Sequence
atgtcggccg aaatcgagtc gcatattctg aagaagtatg agattcaaac acagctaggc cagggtgcct acggcattgt gtggcgcgcc ctggagcgca agcacaaccg cgtcgttgcg ctcaagaaaa tttacgatgc cttccaaaac tcgaccgatg cgcagcgcac cttccgcgag atcatgttcc tgcaccggct gcaccacccg aacatcatcc ggctgctgca tgtccatcgc gccttcaacg atcgcgacat atacctggtc ttcgaataca tggagaccga ccttcatgtg gtgattaggg caaatatctt ggagggaatc cacaaacagt ttatcattta ccagttgctc aagacgatga agtttctgca ttctgccgag attcttcacc gtgacatgaa gccaagcaat ctgctcgtaa acagcgactg cacgatgaag gtggctgact tcggtctcgc gcgctctatc ctgtcccttg agggcgagca ggcgtcgcgg ccggtgctaa cggactacat tgcgacacgg tggtaccgcc cgccggagat tttgctcggc tcgacacgat acactaaggg cgtggatatg tggtcagtgg gctgcattct tggagagttg atgctgggca agcccatgtt tcccggccgc tccaccacga accagctgga gctgatttgc agcgtgacgg gcatgccctc cgcggcggat gtggcagcca ccaactcgca gttcgccaac gcgatgcttc gcgacattca ctgcgcacac cgacgcacgt tcgctgagct gctgccaagc gcttccgcgg atgcgctgaa cctaattgag cgccttatgt gtttcaaccc aaaccgtcgc ttgtctgccg ccgaggctct ggaacacccc tacgtcgctg cctttcaccg tcctgacgac gaaccggtgg cacccgaacc catcaccgtt tctctccctg acagccagcg tttgcccttg gccaagtaca gagacgccat ctacgagcaa atcgcctcgc tgcgccgtat ctccacatcc accgatcatc ggcatcgcgc ggagcgacat ggcgccggcg gcaccgctag ccgcaagacg tccgccagcg gcggcggagg cgctgctggc ggctctcgag gtggcaccgg gacatctggg gccacgcgac ccgccacgag ctgcagcagc agcaccgccg cacgggcggc tccgcagcgc agtgttgtaa agcccacctc gactagtgga gtcaacgaat catcatcttc gaaggcgtac gggcgtcccg gcttccgctc ggtagcgtct accagctctg gtctggaaag cagacccgtc gagcgtgagg cagcggtgcg caaatga
Details for LemeA.01391.a.HZ3.GE43725
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 85
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS AEIESHILXK YEIQTQLGQG AYGIVWRALE RKHNRVVALX KIYDAFXNST DAQRTFREIM FLHXLHXPNI IRLLHVHRAF XDRDXYXXFX XXXXXXSCGX XXQISWRXSX NSLSFTXCSR R
Validated NT Sequence
tccncgcgat gccnatgatc ggtggatgtg nanatacngn gcannnnngn gatttgctcg tanatggcgt ctctgtactt ggccnagggc aaacgctggc tgtcagggag agaaacggtg atgggttcgg gtgccaccgg ntcnncnncn ngacggtgaa aggcancgac gtangggtgt tccanagcct cggcggnnga caagcgacgg tttgggttga aacacataag gcgctcnatt angntcagcg catccnnnga agcgcttggc agcnnntcng cgaacgngcg ncngngtgcn cantgaatgt cnnnnnnnnt cnnnnnnnnn aantgcnant tggtggctgc cncntccgcc gcgnagggca tgcccgtcac nctgcnnntn nnntccnnct ggttcntgnn gnancgncnn nnaaacatgg gcttgcccnn catcaactct ccnnnanngc ancccactgn ncnnntatcc ncnnnnnnan ngnnnnnngn cnagccganc nnntctccng cnnnngnnnn nnccgtgnnn nantgtannc cgttagcacc gnccgcgacn cctgctcnnn nncaagggac angatngnnc gcgcgaganc naagtcagcc acnttcntcn tgcagtcgct gtttacgagc anattgcttg gcttcatgtn ncgnnnaana ntctcggcag aanngcagaa acttcatcgt cttgagcann tggtaaatga taaactgttt gnggatnccc tccaagatat ttgccnnnan tcaccacatg annnntcgnt nnccannnat tngaagnccn ngtatangtc gcgatcgtng aaggcgcgat ggacatgcag cagccggatg atgttcgggt ngtgcagnng gtgcaggaac atgatctcgc ggaaggtgcg ctgcgcatcg gtcgagtttn ggaaggcatc gtaaattttc tngagcgcaa cgacgcggtt gtgcttgcgc tccagggcgc gccacacaat gccgtaggca ccctggccta gctgtgtttg aatctcatac ttnttcagaa tatgcgactc gatttcggcc gacatcgaac caggaccctg ggtctgagct tccagggtac caccaatctg ttctctgtga gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggtctgatc agcttgaatt ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagcgaac gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc ttagcttctt gattgacttc tgagtccgac ttcatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS AEIESHILKK YEIQTQLGQG AYGIVWRALE RKHNRVVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IRLLHVHRAF NDRDIYLVFE YMETDLHVVI RANILEGIHK QFIIYQLLKT MKFLHSAEIL HRDMKPSNLL VNSDCTMKVA DFGLARSILS LEGEQASRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILGELML GKPMFPGRST TNQLELICSV TGMPSAADVA ATNSQFANAM LRDIHCAHRR TFAELLPSAS ADALNLIERL MCFNPNRRLS AAEALEHPYV AAFHRPDDEP VAPEPITVSL PDSQRLPLAK YRDAIYEQIA SLRRISTSTD HRHRAER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtcggccga aatcgagtcg catattctga agaagtatga gattcaaaca cagctaggcc agggtgccta cggcattgtg tggcgcgccc tggagcgcaa gcacaaccgc gtcgttgcgc tcaagaaaat ttacgatgcc ttccaaaact cgaccgatgc gcagcgcacc ttccgcgaga tcatgttcct gcaccggctg caccacccga acatcatccg gctgctgcat gtccatcgcg ccttcaacga tcgcgacata tacctggtct tcgaatacat ggagaccgac cttcatgtgg tgattagggc aaatatcttg gagggaatcc acaaacagtt tatcatttac cagttgctca agacgatgaa gtttctgcat tctgccgaga ttcttcaccg tgacatgaag ccaagcaatc tgctcgtaaa cagcgactgc acgatgaagg tggctgactt cggtctcgcg cgctctatcc tgtcccttga gggcgagcag gcgtcgcggc cggtgctaac ggactacatt gcgacacggt ggtaccgccc gccggagatt ttgctcggct cgacacgata cactaagggc gtggatatgt ggtcagtggg ctgcattctt ggagagttga tgctgggcaa gcccatgttt cccggccgct ccaccacgaa ccagctggag ctgatttgca gcgtgacggg catgccctcc gcggcggatg tggcagccac caactcgcag ttcgccaacg cgatgcttcg cgacattcac tgcgcacacc gacgcacgtt cgctgagctg ctgccaagcg cttccgcgga tgcgctgaac ctaattgagc gccttatgtg tttcaaccca aaccgtcgct tgtctgccgc cgaggctctg gaacacccct acgtcgctgc ctttcaccgt cctgacgacg aaccggtggc acccgaaccc atcaccgttt ctctccctga cagccagcgt ttgcccttgg ccaagtacag agacgccatc tacgagcaaa tcgcctcgct gcgccgtatc tccacatcca ccgatcatcg gcatcgcgcg gagcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa