SSGCID: Target Page

LemeA.00425.a

Elongation factor 1-alpha

CENTER ID:	LemeA.00425.a
ORGANISM:	Leishmania mexicana MHOM/GT/2001/U1103
ASSOCIATED DISEASE:	Leishmaniasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LemeA.00425.a.B1.GE35992	full length		1	449

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:LmxM.17.0080
RefSeq:	XP_003873865.1
RefSeq:	XP_003873866.1
RefSeq:	XP_003873867.1
UniProt:	E9ARD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGKDKVHMNL VVVGHVDAGK STATGHLIYK CGGIDKRTIE KFEKEAAEIG KASFKYAWVL DKLKAERERG ITIDIALWKF ESPKSVFTII DAPGHRDFIK NMITGTSQAD AAILMIDSTH GGFEAGISKD GQTREHALLA FTLGVKQMVV CCNKMDDKTV MYAQSRYDEI SKEVSAYLKR VGYNPEKVRF IPISGWQGDN MIDKSDNMPW YKGPTLLDAL DMLEPPVRPV DKPLRLPLQD VYKIGGIGTV PVGRVETGIM KPGDVVTFAP ANVTTEVKSI EMHHEQLAEA QPGDNVGFNV KNVSVKDIRR GNVCGNSKND PPKEAADFTA QVIVLNHPGQ ISNGYAPVLD CHTSHIACRF AEIESKIDRR SGKELEKNPK AIKSGDAAIV KMVPQKPMCV EVFNDYAPLG RFAVRDMRQT VAVGIIKGVN KKESSGGKVT KAATKAAKK

NT Sequence

atgggcaagg ataaggtgca catgaacctt gtggtcgtcg gccatgtcga cgccggcaag tccaccgcca ctggccactt gatctacaag tgcggtggca tcgacaagcg cacgatcgag aagttcgaga aggaggccgc cgagatcggc aaggcgtcct tcaagtacgc gtgggtgctc gacaagctga aggcggagcg cgagcgcggc atcacgatcg acattgcgct gtggaagttc gagtcgccga agtccgtgtt cacgatcatc gatgcgcccg gccaccgcga cttcatcaag aacatgatca cgggcacgtc gcaggcggac gcggccatcc tgatgatcga ctcgacgcat ggtggcttcg aggctggcat ctcgaaggac ggccagaccc gcgagcacgc gctgcttgcc ttcactcttg gcgtgaagca gatggtggtg tgctgcaaca agatggacga caagacggtg atgtacgcgc agtcgcgcta cgatgagatc agcaaggagg tgagcgcgta cctgaagcgc gtgggctaca acccggagaa ggtgcgcttc atcccgatct cggggtggca gggcgacaac atgatcgaca agtcggacaa catgccgtgg tacaagggtc ccacgctgct ggacgcgctc gacatgctgg agccgccggt gcgcccggtg gacaagccgc tgcgcctgcc cctgcaggac gtgtacaaga tcggcggtat cgggacggtg cccgtgggcc gcgtggagac cgggatcatg aagccgggcg acgtggtgac gttcgcgccc gccaacgtga cgactgaggt gaagtcgatc gagatgcacc acgagcagct ggcggaggcg cagcccggcg acaacgtcgg cttcaacgtg aagaacgtgt cggtgaagga catccgccgt ggtaacgtgt gcggcaactc gaagaacgac ccgccgaagg aggcggccga cttcacggcg caggtgatcg tgctgaacca ccccggccag atcagcaacg gctacgcgcc ggtgctggac tgccacacga gccacatcgc gtgccgcttc gcggagatcg agtccaagat cgaccgccgc tccggcaagg agctggagaa gaaccccaag gcgatcaagt ctggcgacgc cgcgatcgtg aagatggtgc cgcagaagcc gatgtgcgtg gaggtgttca acgactacgc gccgctgggc cgctttgccg tgcgcgacat gcggcagacg gtcgccgtgg gcatcatcaa gggcgtgaac aagaaggaga gcagcggcgg gaaggtgacc aaggcggcca cgaaggctgc gaagaagtaa

Details for LemeA.00425.a.B1.GE35992

HARVESTED ON:	8/28/2012
SEQUENCED ON:	1/7/2013
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	65

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatgggc aaggataagg tgcacatgaa ccttgtggtc gtcggccatg tcgacgccgg caagtccacc gccactggcc acttgatcta caagtgcggt ggcatcgaca agcgcacgat cgagaagttc gagaaggagg ccgccgagat cggcaaggcg tccttcaagt acgcgtgggt gctcgacaag ctgaaggcgg agcgcgagcg cggcatcacg atcgacattg cgctgtggaa gttcgagtcg ccgaagtccg tgttcacgat catcgatgcg cccggccacc gcgacttcat caagaacatg atcacgggca cgtcgcaggc ggacgcggcc atcctgatga tcgactcgac gcatggtggc ttcgaggctg gcatctcgaa ggacggccag acccgcgagc acgcgctgct tgccttcact cttggcgtga agcagatggt ggtgtgctgc aacaagatgg acgacaagac ggtgatgtac gcgcagtcgc gctacgatga gatcagcaag gaggtgagcg cgtacctgaa gcgcgtgggc tacaacccgg agaaggtgcg cttcatcccg atctcggggt ggcagggcga caacatgatc gacaagtcgg acaacatgcc gtggtacaag ggtcccacgc tgctggacgc gctcgacatg ctggagccgc cggtgcgccc ggtggacaag ccgctgcgcc tgcccctgca ggacgtgtac aagatcggcg gtatcgggac ggtgcccgtg ggccgcgtgg agaccgggat catgaagccn ggcgacgtgg tgacgttcgc gcccgccaac gtgacgactg angtgaagtc natcgagatg caccacgagc agctggcgga gnnncagccc ggcna

Expected Protein Sequence

MAHHHHHHMG KDKVHMNLVV VGHVDAGKST ATGHLIYKCG GIDKRTIEKF EKEAAEIGKA SFKYAWVLDK LKAERERGIT IDIALWKFES PKSVFTIIDA PGHRDFIKNM ITGTSQADAA ILMIDSTHGG FEAGISKDGQ TREHALLAFT LGVKQMVVCC NKMDDKTVMY AQSRYDEISK EVSAYLKRVG YNPEKVRFIP ISGWQGDNMI DKSDNMPWYK GPTLLDALDM LEPPVRPVDK PLRLPLQDVY KIGGIGTVPV GRVETGIMKP GDVVTFAPAN VTTEVKSIEM HHEQLAEAQP GDNVGFNVKN VSVKDIRRGN VCGNSKNDPP KEAADFTAQV IVLNHPGQIS NGYAPVLDCH TSHIACRFAE IESKIDRRSG KELEKNPKAI KSGDAAIVKM VPQKPMCVEV FNDYAPLGRF AVRDMRQTVA VGIIKGVNKK ESSGGKVTKA ATKAAKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca aggataaggt gcacatgaac cttgtggtcg tcggccatgt cgacgccggc aagtccaccg ccactggcca cttgatctac aagtgcggtg gcatcgacaa gcgcacgatc gagaagttcg agaaggaggc cgccgagatc ggcaaggcgt ccttcaagta cgcgtgggtg ctcgacaagc tgaaggcgga gcgcgagcgc ggcatcacga tcgacattgc gctgtggaag ttcgagtcgc cgaagtccgt gttcacgatc atcgatgcgc ccggccaccg cgacttcatc aagaacatga tcacgggcac gtcgcaggcg gacgcggcca tcctgatgat cgactcgacg catggtggct tcgaggctgg catctcgaag gacggccaga cccgcgagca cgcgctgctt gccttcactc ttggcgtgaa gcagatggtg gtgtgctgca acaagatgga cgacaagacg gtgatgtacg cgcagtcgcg ctacgatgag atcagcaagg aggtgagcgc gtacctgaag cgcgtgggct acaacccgga gaaggtgcgc ttcatcccga tctcggggtg gcagggcgac aacatgatcg acaagtcgga caacatgccg tggtacaagg gtcccacgct gctggacgcg ctcgacatgc tggagccgcc ggtgcgcccg gtggacaagc cgctgcgcct gcccctgcag gacgtgtaca agatcggcgg tatcgggacg gtgcccgtgg gccgcgtgga gaccgggatc atgaagccgg gcgacgtggt gacgttcgcg cccgccaacg tgacgactga ggtgaagtcg atcgagatgc accacgagca gctggcggag gcgcagcccg gcgacaacgt cggcttcaac gtgaagaacg tgtcggtgaa ggacatccgc cgtggtaacg tgtgcggcaa ctcgaagaac gacccgccga aggaggcggc cgacttcacg gcgcaggtga tcgtgctgaa ccaccccggc cagatcagca acggctacgc gccggtgctg gactgccaca cgagccacat cgcgtgccgc ttcgcggaga tcgagtccaa gatcgaccgc cgctccggca aggagctgga gaagaacccc aaggcgatca agtctggcga cgccgcgatc gtgaagatgg tgccgcagaa gccgatgtgc gtggaggtgt tcaacgacta cgcgccgctg ggccgctttg ccgtgcgcga catgcggcag acggtcgccg tgggcatcat caagggcgtg aacaagaagg agagcagcgg cgggaaggtg accaaggcgg ccacgaaggc tgcgaagaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat